An integrated spatio-temporal view of riverine biodiversity using environmental DNA metabarcoding.

Anthropogenically forced changes in global freshwater biodiversity demand more efficient monitoring approaches. Consequently, environmental DNA (eDNA) analysis is enabling ecosystem-scale biodiversity assessment, yet the appropriate spatio-temporal resolution of robust biodiversity assessment remains ambiguous. Here, using intensive, spatio-temporal eDNA sampling across space (five rivers in Europe and North America, with an upper range of 20-35 km between samples), time (19 timepoints between 2017 and 2018) and environmental conditions (river flow, pH, conductivity, temperature and rainfall), we characterise the resolution at which information on diversity across the animal kingdom can be gathered from rivers using eDNA. In space, beta diversity was mainly dictated by turnover, on a scale of tens of kilometres, highlighting that diversity measures are not confounded by eDNA from upstream. Fish communities showed nested assemblages along some rivers, coinciding with habitat use. Across time, seasonal life history events, including salmon and eel migration, were detected. Finally, effects of environmental conditions were taxon-specific, reflecting habitat filtering of communities rather than effects on DNA molecules. We conclude that riverine eDNA metabarcoding can measure biodiversity at spatio-temporal scales relevant to species and community ecology, demonstrating its utility in delivering insights into river community ecology during a time of environmental change.

Cross-continental comparative experiences of wastewater surveillance and a vision for the 21st century.

The COVID-19 pandemic has brought the epidemiological value of monitoring wastewater into sharp focus. The challenges of implementing and optimising wastewater monitoring vary significantly from one region to another, often due to the array of different wastewater systems around the globe, as well as the availability of resources to undertake the required analyses (e.g. laboratory infrastructure and expertise). Here we reflect on the local and shared challenges of implementing a SARS-CoV-2 monitoring programme in two geographically and socio-economically distinct regions, São Paulo state (Brazil) and Wales (UK), focusing on design, laboratory methods and data analysis, and identifying potential guiding principles for wastewater surveillance fit for the 21st century. Our results highlight the historical nature of region-specific challenges to the implementation of wastewater surveillance, including previous experience of using wastewater surveillance, stakeholders involved, and nature of wastewater infrastructure. Building on those challenges, we then highlight what an ideal programme would look like if restrictions such as resource were not a constraint. Finally, we demonstrate the value of bringing multidisciplinary skills and international networks together for effective wastewater surveillance.

Addressing the challenges of combined sewer overflows.

Europe's ageing wastewater system often combines domestic sewage with surface runoff and industrial wastewaters. To reduce the associated risk of overloading wastewater treatment works during storms, and to prevent wastewater backing-up into properties, Combined Sewer Overflows (CSOs) are designed into wastewater networks to release excess discharge into rivers or coastal waters without treatment. In view of growing regulatory scrutiny and increasing public concern about their excessive discharge frequencies and potential impacts on environments and people, there is a need to better understand these impacts to allow prioritisation of cost-effective solutions.We review: i) the chemical, physical and biological composition of CSOs discharges; ii) spatio-temporal variations in the quantity, quality and load of overflows spilling into receiving waters; iii) the potential impacts on people, ecosystems and economies. Despite investigations illustrating the discharge frequency of CSOs, data on spill composition and loading of pollutants are too few to reach representative conclusions, particularly for emerging contaminants. Studies appraising impacts are also scarce, especially in contexts where there are multiple stressors affecting receiving waters. Given the costs of addressing CSOs problems, but also the likely long-term gains (e.g. economic stimulation as well as improvements to biodiversity, ecosystem services, public health and wellbeing), we highlight here the need to bolster these evidence gaps. We also advocate no-regrets options to alleviate CSO problems taking into consideration economic costs, carbon neutrality, ecosystem benefit and community well-being. Besides pragmatic, risk-based investment by utilities and local authorities to modernise wastewater systems, these include i) more systemic thinking, linking policy makers, consumers, utilities and regulators, to shift from local CSO issues to integrated catchment solutions with the aim of reducing contributions to wastewater from surface drainage and water consumption; ii) broader societal responsibilities for CSOs, for example through improved regulation, behavioural changes in water consumption and disposal of waste into wastewater networks, and iii) greater cost-sharing of wastewater use.

The environmental impact of keeping a tropical aquarium in Northern Europe.

Tropical fishkeeping is a popular practice in societies across the globe and involves recreating and sustaining an entire ecosystem in an aquarium within a domestic setting. The process invariably has an environmental impact, yet an assessment of this impact has previously been limited to the ecological consequences of harvesting fish from the wild or the release of non-native fish species. Provided here are the first estimates of carbon dioxide equivalent (CO2 eq) emissions produced from running a tropical aquarium across multiple countries in Northern Europe (France, Poland and the UK), along with water consumption. Estimates were produced in silico and are discussed in the context of freshwater and marine aquariums, calculated using example aquarium sizes of 50, 200 and 400 l. Using estimates from the UK, depending on size and running conditions, a tropical aquarium produces an estimated 85.3-635.2 kg of CO2 eq per year, equating to 1.6%-12.4% of the UK annual average household CO2 emissions, and uses 156-31,200 l of water per year, equating to 0.2%-30.1% of the UK annual average household water usage. Despite this, comparison with the CO2 eq of an average-size dog (127-1592 kg of CO2 eq per year) or cat (121-251 kg of CO2 eq per year), estimated from meat consumption alone, demonstrates that ornamental fishkeeping can be a more environmentally conscious pet choice. In addition, the majority of CO2 eq produced from tropical fishkeeping is generated from the energy consumption of aquarium equipment and as more national electricity grids begin to decarbonize, this estimate should decrease.