RESUMO
BACKGROUND: Prominent inflammatory infiltrates of macrophages and T-lymphocytes are found in both aortic occlusive disease (AOD) and abdominal aortic aneurysms (AAA). These cells secrete different cytokines that might affect matrix turnover through modulation of matrix metalloproteinase expression. A different cytokine pattern might account for the evolution of AOD vs AAA. MATERIALS AND METHODS: Six different cytokines were examined to determine whether AOD and AAA could be characterized by unique cytokine patterns. AOD (n = 8) and AAA (n = 8) tissues were collected and serially treated with salt, dimethyl sulfoxide, and urea buffers to extract the soluble matrix or cell-bound cytokines. Levels of IL-1 beta, TNF-alpha, IL-10, IL-12, and IFN-gamma were measured by immunoenzymatic methods. Additionally, RNA levels of IL-12 and IFN-gamma were measured. RESULTS: AAA tissue contained higher levels of IL-10 compared to AOD tissue (P < 0.05). Higher levels of the proinflammatory cytokines IL-1 beta, TNF-alpha, and IL-6 were found in AOD (P < 0.05). mRNA levels of IL-12 and IFN-gamma did not differ between the diseases. Aortic tissues contained large amounts of matrix or cell-bound cytokines. CONCLUSIONS: AAA is characterized by greater levels of IL-10 while IL-1 beta, TNF-alpha, and IL-6 are higher in AOD. Targeted deletion of these cytokines in animal models might help in identifying their role in the progression of AAA.
Assuntos
Aneurisma da Aorta Abdominal/imunologia , Doenças da Aorta/imunologia , Arteriopatias Oclusivas/imunologia , Citocinas/análise , Citocinas/genética , Feminino , Humanos , Interleucina-1/análise , Interleucina-1/genética , Interleucina-10/análise , Interleucina-10/genética , Interleucina-6/análise , Interleucina-6/genética , Masculino , RNA Mensageiro/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fator de Necrose Tumoral alfa/análise , Fator de Necrose Tumoral alfa/genéticaRESUMO
It is shown that unithiol protects mice from death in intoxication by S. typhimurium and S. Sonnei lysates and in infection caused by S. typhimurium. Magnesium sulfate strengthens the protective effect of unithiol. It is suggested that the protective effect of unithiol is due to its antioxidant action.
Assuntos
Endotoxinas/intoxicação , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Sulfato de Magnésio/uso terapêutico , Unitiol/uso terapêutico , Animais , Quimioterapia Combinada , Sulfato de Magnésio/administração & dosagem , Masculino , Camundongos , Camundongos Endogâmicos CBA , Unitiol/administração & dosagemRESUMO
The experiments were carried out on mice of CBA strain. The animals received lysate of S. typhimurium in the dose of LD50. The injection of lysate was followed by an increase in the level of malonic dialdehyde (MDA) suggesting the lipid peroxidation (LPO) activation in the liver. At the same time the concentration of cyclic adenosine monophosphate (cAMP) in the liver and the lungs was decreasing, which was indicative of the decrease in the activity of prostaglandins. The injections of unithiol prevented both the activation of LPO and the decrease in the concentration of cAMP, that effect having been caused by the antioxidant action of unithiol. The repeated injection of unithiol protected the animals from death in intoxication by lysate of S. typhimurium and Shigella sonnei; the protection manifested itself in the 4.2 and 3.7-fold increase in LD50 respectively. Magnesium sulphate enhanced the protective action of unithiol.