[Growth features of CHO cells in culture].

Dependence of growth characteristics of cell population and the monolayer formation on the initial plating concentrations of cells were studied on the established CHO cell line. The cells were cultivated under standard conditions on plastic substrate. Initial plating concentration was varied as: 1000, 2000, 3000, 4000, 5000, and 6000 cell/cm2. It was shown that growth of the cell culture can be formally described by standard S-shaped dependence. However, a more detailed analysis revealed a discrepancy between the experimental and expected data. Specifically the arrest of cell growth at the monolayer formation does not coincide with the time when the theoretical curves approach plateau. It has been concluded that cell proliferation and the formation of a monolayer are independent processes (at last in CHO cells). Both processes may be considered as analogues of proliferation and morphogenesis in metazoan. In addition, it has been shown that the arrest of cell division occurs not only by contact inhibition of proliferation during monolayer formation but also by reducing the size of cells to some limiting dimension and by increasing the polarization of cells.

[The technology for manufacturing antiparasitic preparations. 8. The preparation rilanid and its use for treating fascioliasis and intestinal nematodiases]. / Tekhnologiia proizvodstva protivoparazitarnykh preparatov. 8. Preparat rilanid i ego primenenie dlia lecheniia fastsioleza i kishechnykh nematodozov.

A procedure has been developed to prepare the anthelminthic Rilanide as a fine-dispersed formulation. The therapeutical dose of the agent in fascioliasis and intestinal nematodiasis is 60 mg/kg (for sheep), if given alone, and 100 mg/kg, if added to feed.

[The technology for manufacturing antiparasitic preparations. 7. Preparation G-1460 and its use for the treatment of monieziasis and intestinal nematodiases]. / Tekhnologiia proizvodstva protivoparazitarnykh preparatov. 7. Preparat G-1460 i ego primenenie dlia lecheniia moniezioza i kishechnykh nematodozov.

A procedure was developed for the synthesis of the anthelminthic G-1460. The therapeutical doses (20 and 25 mg/kg) of the agent were defined for the treatment of monieziasis and gastrointestinal nematodes on an individual basis.

[The search for new antiparasitic agents. 16. A study of the anti-Fasciola activity of N-[(chloro-naphthyloxy)phenyl]benzamides]. / Poisk novykh protivoparazitarnykh sredstv. 16. Izuchenie protivofastsioleznoi aktivnosti nekotorykh N-[(khlor-naftiloksi)fenil]benzamidov.

The study was undertaken to examine the fasciolacidal activity of the agents G-1411, G-1423, and G-1439. Tested, G-1439 was chosen for detailed investigations as a non-toxic fasciolacide.

[The technology for manufacturing antiparasitic preparations. 5. The development of a technology for producing the anthelmintic triclonate and the evaluation of its therapeutic efficacy in monieziasis]. / Tekhnologiia proizvodstva protivoparazitarnykh preparatov. 5. Razrabotka tekhnologii polucheniia antigel'mintika triklonata i otsenka ego lechebnoi éffektivnosti pri moniezioze.

A procedure has been developed to manufacture the anthelminthic Triclonate. The agent was tested for the treatment of sheep's natural monieziasis infection and it was found to have a high activity.

[The technology for manufacturing antiparasitic preparations. 4. The development of a technology for producing the anthelmintic bromoksan in finely dispersed form and the evaluation of its antifascioliasis activity]. / Tekhnologiia proizvodstva protivoparazitarnykh preparatov. 4. Razrabotka tekhnologii polucheniia antigel'mintika bromoksana v melkodispersnoi forme i otsenka ego protivofastsioleznoi aktivnosti.

A manufacturing procedure has been developed to prepare the anthelmintic bromoxane as a finely divided dosage form. The maximum toxic dose of the drug was 1 g per kg mice body weight. Its therapeutic dose in sheep fascioliasis was 20 mg/kg.

[The technology for manufacturing antiparasitic preparations. 1. The development of a technology for producing the anthelmintic triklazan in finely dispersed form and the evaluation of its antinematodal, anticestodal and antitrematodal activities]. / Tekhnologiia proizvodstva protivoparazitarnykh preparatov. Soobshchenie 1. Razrabotka tekhnologii polucheniia antigel'mintika triklazana v melkodispersnoi forme i otsenka ego protivonematodnoi, protivotsestodnoi i protivotrematodnoi aktivnosti.

The authors describe the technology of preparing a new anthelmintic agent triclazan, N-(3,4-dichlorophenyl)-2-[(benzo-2,1,3-thiadiazole-4-sulfonyl)amino]-5- chlorobenzamide, finely dispersed (the particles size 4-8 mu). Triclazan is highly effective not only in hymenolepiasis and trichocephaliasis, as was shown previously, but in fascioliasis, monieziasis and intestinal nematodiasis of sheep as well.