RESUMO
The rice disease resistance gene Xa21, which encodes a receptor-like kinase, is a member of a multigene family. Based on comparisons of genomic sequences of seven family members, seventeen transposon-like elements were identified in the 5' and 3' flanking regions and introns of these genes. Sequence characterization revealed that these elements are diverse, showing similarity to maize Ds, CACTA and miniature inverted repeat-like elements, as well as novel elements. Only two elements were located in presumed coding regions, indicating that integration of transposable elements at the Xa21 disease resistance locus occurred preferentially in noncoding regions.
Assuntos
Elementos de DNA Transponíveis/genética , Oryza/genética , Doenças das Plantas/genética , Proteínas de Plantas/genética , Proteínas Serina-Treonina Quinases/genética , Sequência de Aminoácidos , Sequência de Bases , Íntrons/genética , Dados de Sequência Molecular , Família Multigênica/genética , Sequências Repetitivas de Ácido Nucleico/genética , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido NucleicoRESUMO
The rice Xa21 gene confers resistance to Xanthomonas oryzae pv oryzae in a race-specific manner. Analysis of the inheritance patterns and resistance spectra of transgenic plants carrying six Xa21 gene family members indicated that one member, designated Xa21D, displayed a resistance spectrum identical to that observed for Xa21 but conferred only partial resistance. Xa21D encodes a receptor-like protein carrying leucine-rich repeat (LRR) motifs in the presumed extracellular domain. The Xa21D transcript terminates shortly after the stop codon introduced by the retrotransposon Retrofit. Comparison of nucleotide substitutions in the LRR coding regions of Xa21 and Xa21D provided evidence of adaptive selection. Both functional and evolutionary evidence indicates that the Xa21D LRR domain controls race-specific pathogen recognition.
Assuntos
Evolução Biológica , Família Multigênica , Oryza/genética , Proteínas de Plantas/biossíntese , Proteínas de Plantas/genética , Proteínas Serina-Treonina Quinases/biossíntese , Proteínas Serina-Treonina Quinases/genética , Xanthomonas/patogenicidade , Adaptação Fisiológica , Sequência de Aminoácidos , Dimerização , Suscetibilidade a Doenças , Evolução Molecular , Leucina , Dados de Sequência Molecular , Oryza/microbiologia , Oryza/fisiologia , Doenças das Plantas , Proteínas de Plantas/química , Plantas Geneticamente Modificadas , Reação em Cadeia da Polimerase , Proteínas Serina-Treonina Quinases/química , Receptores de Superfície Celular/biossíntese , Receptores de Superfície Celular/genética , Retroelementos , Alinhamento de Sequência , Xanthomonas/genéticaRESUMO
The rice disease resistance gene Xa21, encoding a receptor-like kinase, is a member of a multigene family. Sequence analysis of seven family members revealed two distinct classes of genes. One member from each class encodes a receptor kinase-like open reading frame. The other five members encode truncated open reading frames of the predicted receptor kinase. A highly conserved 233-bp sequence (HC) was also identified among the seven family members. Recombination at the HC region between family members apparently resulted in the precise swapping of promoter regions. Large sequence duplications were generated by a presumed unequal crossover event in intergenic regions. Insertions of transposon-like sequences truncated two of the predicted open reading frames. A model for amplification and diversification of the Xa21 gene family is presented.
Assuntos
Evolução Molecular , Genes de Plantas , Família Multigênica , Oryza/genética , Proteínas de Plantas/genética , Proteínas Serina-Treonina Quinases/genética , Sequência de Aminoácidos , Composição de Bases , Sequência de Bases , Clonagem Molecular , Sequência Conservada , Elementos de DNA Transponíveis , DNA de Plantas/química , DNA de Plantas/genética , Modelos Genéticos , Dados de Sequência Molecular , Oryza/enzimologia , Recombinação GenéticaRESUMO
The rice Xa21 gene, which confers resistance to Xanthomonas oryzae pv. oryzae race 6, was isolated by positional cloning. Fifty transgenic rice plants carrying the cloned Xa21 gene display high levels of resistance to the pathogen. The sequence of the predicted protein, which carries both a leucine-rich repeat motif and a serine-threonine kinase-like domain, suggests a role in cell surface recognition of a pathogen ligand and subsequent activation of an intracellular defense response. Characterization of Xa21 should facilitate understanding of plant disease resistance and lead to engineered resistance in rice.