RESUMO
The association of ESR1 PvuII polymorphism (rs2334693) with impaired ovarian reserve was studied by genotyping this polymorphism using PCR-RFLP in patients and two control groups from Ukraine. Statistically significant differences in the prevalence of p-allele frequency (-397T) was seen in the group of patients with impaired ovarian reserve (0.597) compared to control groups I under 35years (0.480) and II over 35years (0.453), both p<0.05. The data suggest that PvuII polymorphism of ESR1 is associated with diminished ovarian reserve.
Assuntos
Receptor alfa de Estrogênio/genética , Insuficiência Ovariana Primária/genética , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Oogênese , Polimorfismo Genético , UcrâniaRESUMO
PURPOSE: To evaluate the association between phenotype and follicle stimulating hormone receptor (FSHR) genotype in women with ovarian dysfunction and patients with "poor response" to gonadotropin stimulation of ovulation. METHODS: FSHR gene SNPs were analyzed by PCR and RFLP. "Poor responders" (ovarian dysfunction) group and "good responders" group constituted the study group. Normo-ovulatory women who gave birth to naturally conceived children formed control groups: under 35 years of age (control I) and over 35 years of age (control II). RESULTS: The frequency of Ala307-Ser680/Ala307-Ser680 genotype was significantly more prevalent in the ovarian dysfunction group (26%) compared to the control I (7.7%) (P < 0.001) and a "good responders" group (12.5%) (P < 0.05); and in a "poor responders" group (33.3%) compared to a "good responders" group (P < 0.05), control I (P < 0.001) and control II (17.5%) (P < 0.05). CONCLUSIONS: Our data shows the prevalence of the Ala307-Ser680/ Ala307-Ser680 genotype in the both groups of patients. The finding should have impact on the delineation of stimulation protocols.