RESUMO
A distinct feature of the nervous system is the intricate network of synaptic connections among neurons of diverse phenotypes. Although initial connections are formed largely through molecular mechanisms that depend on intrinsic developmental programs, spontaneous and experience-driven electrical activities in the developing brain exert critical epigenetic influence on synaptic maturation and refinement of neural circuits. Selective findings discussed here illustrate some of our current understanding of the effects of electrical activity on circuit development and highlight areas that await further study.
Assuntos
Potenciais de Ação/genética , Padronização Corporal/genética , Comunicação Celular/genética , Sistema Nervoso Central/embriologia , Vias Neurais/embriologia , Sinapses/genética , Animais , Sistema Nervoso Central/citologia , Sistema Nervoso Central/metabolismo , Cones de Crescimento/metabolismo , Cones de Crescimento/ultraestrutura , Humanos , Fatores de Crescimento Neural/genética , Vias Neurais/citologia , Vias Neurais/metabolismo , Sinapses/metabolismo , Sinapses/ultraestruturaRESUMO
The role of neurotrophins as regulatory factors that mediate the differentiation and survival of neurons has been well described. More recent evidence indicates that neurotrophins may also act as synaptic modulators. Here, I review the evidence that synaptic activity regulates the synthesis, secretion and action of neurotrophins, which can in turn induce immediate changes in synaptic efficacy and morphology. By this account, neurotrophins may participate in activity-dependent synaptic plasticity, linking synaptic activity with long-term functional and structural modification of synaptic connections.
Assuntos
Fatores de Crescimento Neural/fisiologia , Neurônios/fisiologia , Sinapses/fisiologia , Animais , Humanos , Plasticidade Neuronal , Neurônios/citologia , Transmissão Sináptica/fisiologiaRESUMO
Activity-induced synaptic modification is essential for the development and plasticity of the nervous system. Repetitive correlated activation of pre- and postsynaptic neurons can induce persistent enhancement or decrement of synaptic efficacy, commonly referred to as long-term potentiation or depression (LTP or LTD). An important unresolved issue is whether and to what extent LTP and LTD are restricted to the activated synapses. Here we show that, in the CA1 region of the hippocampus, reduction of postsynaptic calcium influx by partial blockade of NMDA (N-methyl-D-aspartate) receptors results in a conversion of LTP to LTD and a loss of input specificity normally associated with LTP, with LTD appearing at heterosynaptic inputs. The induction of LTD at homo- and heterosynaptic sites requires functional ryanodine receptors and inositol triphosphate (InsP3) receptors, respectively. Functional blockade or genetic deletion of type 1 InsP3 receptors led to a conversion of LTD to LTP and elimination of heterosynaptic LTD, whereas blocking ryanodine receptors eliminated only homosynaptic LTD. Thus, postsynaptic Ca2+, deriving from Ca2+ influx and differential release of Ca2+ from internal stores through ryanodine and InsP3 receptors, regulates both the polarity and input specificity of activity-induced synaptic modification.
Assuntos
Cálcio/fisiologia , Sinapses/fisiologia , Animais , Canais de Cálcio/fisiologia , Potenciais Pós-Sinápticos Excitadores , Hipocampo/fisiologia , Técnicas In Vitro , Inositol 1,4,5-Trifosfato/fisiologia , Receptores de Inositol 1,4,5-Trifosfato , Potenciação de Longa Duração/fisiologia , Masculino , Modelos Neurológicos , N-Metilaspartato/antagonistas & inibidores , N-Metilaspartato/fisiologia , Neurônios/fisiologia , Ratos , Receptores Citoplasmáticos e Nucleares/fisiologiaRESUMO
We describe a novel bioassay system that uses Xenopus embryonic myocytes (myoballs) to detect the release of acetylcholine from Drosophila CNS neurons. When a voltage-clamped Xenopus myoball was manipulated into contact with cultured Drosophila "giant" neurons, spontaneous synaptic current-like events were registered. These events were observed within seconds after contact and were blocked by curare and alpha-bungarotoxin, but not by TTX and Cd(2+), suggesting that they are caused by the spontaneous quantal release of acetylcholine (ACh). The secretion occurred not only at the growth cone, but also along the neurite and at the soma, with significantly different release parameters among various regions. The amplitude of these currents displayed a skewed distribution. These features are distinct from synaptic transmission at more mature synapses or autapses formed in this culture system and are reminiscent of the transmitter release process during early development in other preparations. The usefulness of this coculture system in studying presynaptic secretion mechanisms is illustrated by a series of studies on the cAMP pathway mutations, dunce (dnc) and PKA-RI, which disrupt a cAMP-specific phosphodiesterase and the regulatory subunit of cAMP-dependent protein kinase A, respectively. We found that these mutations affected the ACh current kinetics, but not the quantal ACh packet, and that the release frequency was greatly enhanced by repetitive neuronal activity in dnc, but not wild-type, growth cones. These results suggest that the cAMP pathway plays an important role in the activity-dependent regulation of transmitter release not only in mature synapses as previously shown, but also in developing nerve terminals before synaptogenesis.
Assuntos
Acetilcolina/metabolismo , AMP Cíclico/fisiologia , Mutação , Neurônios/metabolismo , Animais , Bungarotoxinas/farmacologia , Cádmio/farmacologia , Células Cultivadas , Curare/farmacologia , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Drosophila melanogaster , Estimulação Elétrica , Fenótipo , Transmissão Sináptica , Tetrodotoxina/farmacologia , Xenopus laevisRESUMO
Recent studies have implicated brain-derived neurotrophic factor (BDNF) in use-dependent modification of hippocampal synapses. BDNF can rapidly potentiate synaptic transmission at glutamatergic synapses by enhancing transmitter release. Using simultaneous perforated patch recording from pairs and triplets of glutamatergic hippocampal neurons, we have examined how the initial state of the glutamatergic synapse determines its susceptibility to synaptic modification by BDNF. We found that the degree of synaptic potentiation by BDNF depends on the initial reliability and strength of the synapse: Relatively weak connections were strongly potentiated, whereas the effect was markedly reduced at stronger synapses. The degree of BDNF-induced potentiation strongly correlated with the initial coefficient of variation (CV) of the amplitude of excitatory postsynaptic currents (EPSCs) and inversely correlated with the initial paired-pulse facilitation, suggesting that synapses with lower release probability (Pr) are more susceptible to the action of BDNF. To determine whether saturation of Pr could have masked the potentiation effect of BDNF in the stronger synapses, we lowered the initial Pr either by reducing the extracellular Ca2+ concentration ([Ca2+]o) or by bath application of adenosine. Synapses that were initially strong remained unaffected by BDNF under these conditions of reduced Pr. Thus, the lack of BDNF effect on synaptic efficacy cannot simply be accounted for by saturation of Pr, but rather may be due to intrinsic changes associated with synaptic maturation that might covary with Pr. Finally, the dependence on initial synaptic strength was also found for divergent outputs of the same presynaptic neuron, suggesting that synaptic terminals with different degrees of responsiveness to BDNF can coexist within in the same neuron.
Assuntos
Fator Neurotrófico Derivado do Encéfalo/farmacologia , Plasticidade Neuronal/fisiologia , Sinapses/efeitos dos fármacos , Sinapses/fisiologia , Transmissão Sináptica/fisiologia , Animais , Células Cultivadas , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Ácido Glutâmico/fisiologia , Hipocampo/citologia , Hipocampo/efeitos dos fármacos , Humanos , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Plasticidade Neuronal/efeitos dos fármacos , Técnicas de Patch-Clamp , Ratos , Proteínas Recombinantes/farmacologia , Transmissão Sináptica/efeitos dos fármacos , Ácido gama-Aminobutírico/fisiologiaRESUMO
Netrins are bifunctional: they attract some axons and repel others. Netrin receptors of the Deleted in Colorectal Cancer (DCC) family are implicated in attraction and those of the UNC5 family in repulsion, but genetic evidence also suggests involvement of the DCC protein UNC-40 in some cases of repulsion. To test whether these proteins form a receptor complex for repulsion, we studied the attractive responses of Xenopus spinal axons to netrin-1, which are mediated by DCC. We show that attraction is converted to repulsion by expression of UNC5 proteins in these cells, that this repulsion requires DCC function, that the UNC5 cytoplasmic domain is sufficient to effect the conversion, and that repulsion can be initiated by netrin-1 binding to either UNC5 or DCC. The isolated cytoplasmic domains of DCC and UNC5 proteins interact directly, but this interaction is repressed in the context of the full-length proteins. We provide evidence that netrin-1 triggers the formation of a receptor complex of DCC and UNC5 proteins and simultaneously derepresses the interaction between their cytoplasmic domains, thereby converting DCC-mediated attraction to UNC5/DCC-mediated repulsion.
Assuntos
Axônios/fisiologia , Proteínas de Caenorhabditis elegans , Moléculas de Adesão Celular/metabolismo , Proteínas de Helminto/metabolismo , Proteínas de Membrana/metabolismo , Receptores de Superfície Celular/metabolismo , Receptores de Fatores de Crescimento/metabolismo , Proteínas Supressoras de Tumor , Sequência de Aminoácidos , Animais , Sítios de Ligação , Células COS , Linhagem Celular , Citoplasma/metabolismo , Receptor DCC , Cones de Crescimento/fisiologia , Proteínas de Helminto/genética , Ligantes , Proteínas de Membrana/genética , Camundongos , Dados de Sequência Molecular , Fatores de Crescimento Neural/metabolismo , Receptores de Netrina , Netrina-1 , Ratos , Receptores de Superfície Celular/genética , Receptores de Fatores de Crescimento/genética , Xenopus/embriologiaRESUMO
Many diffusible axon guidance cues and their receptors have been identified recently. These cues are often found to be bifunctional, acting as attractants or repellents under different circumstances. Studies of cytoplasmic signaling mechanisms have led to the notion that the response of a growth cone to a particular guidance cue depends on the internal state of the neuron, which, in turn, is under the influence of other coincident signals received by the neuron. Furthermore, many diffusible guidance cues appear to share common cytoplasmic signaling pathways.
Assuntos
Cones de Crescimento/fisiologia , Fatores de Crescimento Neural/metabolismo , Transdução de Sinais/fisiologia , Animais , Cálcio/metabolismo , Citoplasma/metabolismo , Difusão , Proteínas da Matriz Extracelular/metabolismoRESUMO
Neurotrophins have been implicated in activity-dependent synaptic plasticity, but the underlying intracellular mechanisms remain largely unknown. Synaptic potentiation induced by brain-derived neurotrophic factor (BDNF), but not neurotrophin 3, was prevented by blockers of adenosine 3',5'-monophosphate (cAMP) signaling. Activators of cAMP signaling alone were ineffective in modifying synaptic efficacy but greatly enhanced the potentiation effect of BDNF. Blocking cAMP signaling abolished the facilitation of BDNF-induced potentiation by presynaptic activity. Thus synaptic actions of BDNF are gated by cAMP. Activity and other coincident signals that modulate cAMP concentrations may specify the action of secreted neurotrophins on developing nerve terminals.
Assuntos
Fator Neurotrófico Derivado do Encéfalo/farmacologia , Carbazóis , AMP Cíclico/fisiologia , Potenciais Pós-Sinápticos Excitadores , Sinapses/fisiologia , Transmissão Sináptica , Animais , Células Cultivadas , AMP Cíclico/análogos & derivados , AMP Cíclico/farmacologia , Cicloleucina/análogos & derivados , Cicloleucina/farmacologia , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Indóis/farmacologia , Fatores de Crescimento Neural/farmacologia , Plasticidade Neuronal , Neurônios/citologia , Neurônios/fisiologia , Neurotrofina 3 , Ácido Okadáico/farmacologia , Técnicas de Patch-Clamp , Pirróis/farmacologia , Transdução de Sinais , Sinapses/efeitos dos fármacos , Transmissão Sináptica/efeitos dos fármacos , Tionucleotídeos/farmacologia , XenopusRESUMO
Neurotrophins have been proposed to participate in activity-dependent modifications of neuronal connectivity and synaptic efficacy. Preferential strengthening of active inputs requires restriction of putative neurotrophin-mediated synaptic potentiation to active synapses. Here we report that potentiation of synaptic efficacy by brain-derived neurotrophic factor (BDNF) is greatly facilitated by presynaptic depolarization at developing neuromuscular synapses. Brief depolarization in the presence of low-level BDNF results in a marked potentiation of both evoked and spontaneous synaptic transmission, whereas exposure to either BDNF or depolarization alone is without effect. This potentiation depends on the relative timing of depolarization and reflects an enhancement of transmitter secretion from the presynaptic neuron. Thus synapses made by active inputs may be selectively strengthened by secreted neurotrophins as part of activity-dependent refinement of developing connections or of mature synapses.
Assuntos
Fator Neurotrófico Derivado do Encéfalo/farmacologia , Junção Neuromuscular/efeitos dos fármacos , Transmissão Sináptica/fisiologia , Potenciais de Ação , Animais , Cálcio/fisiologia , Células Cultivadas , Potenciais Evocados/efeitos dos fármacos , Potenciais da Membrana/efeitos dos fármacos , Junção Neuromuscular/fisiologia , Técnicas de Patch-Clamp , Potássio/farmacologia , Transmissão Sináptica/efeitos dos fármacos , Xenopus laevisRESUMO
In cultures of dissociated rat hippocampal neurons, persistent potentiation and depression of glutamatergic synapses were induced by correlated spiking of presynaptic and postsynaptic neurons. The relative timing between the presynaptic and postsynaptic spiking determined the direction and the extent of synaptic changes. Repetitive postsynaptic spiking within a time window of 20 msec after presynaptic activation resulted in long-term potentiation (LTP), whereas postsynaptic spiking within a window of 20 msec before the repetitive presynaptic activation led to long-term depression (LTD). Significant LTP occurred only at synapses with relatively low initial strength, whereas the extent of LTD did not show obvious dependence on the initial synaptic strength. Both LTP and LTD depended on the activation of NMDA receptors and were absent in cases in which the postsynaptic neurons were GABAergic in nature. Blockade of L-type calcium channels with nimodipine abolished the induction of LTD and reduced the extent of LTP. These results underscore the importance of precise spike timing, synaptic strength, and postsynaptic cell type in the activity-induced modification of central synapses and suggest that Hebb's rule may need to incorporate a quantitative consideration of spike timing that reflects the narrow and asymmetric window for the induction of synaptic modification.
Assuntos
Potenciais de Ação/fisiologia , Hipocampo/fisiologia , Neurônios/fisiologia , Sinapses/fisiologia , 6-Ciano-7-nitroquinoxalina-2,3-diona/farmacologia , Potenciais de Ação/efeitos dos fármacos , Animais , Bicuculina/farmacologia , Canais de Cálcio/efeitos dos fármacos , Canais de Cálcio/fisiologia , Células Cultivadas , Embrião de Mamíferos , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Ácido Glutâmico/fisiologia , Técnicas In Vitro , Potenciação de Longa Duração , Neurônios/efeitos dos fármacos , Nimodipina/farmacologia , Técnicas de Patch-Clamp , Ratos , Sinapses/efeitos dos fármacos , Fatores de Tempo , Ácido gama-Aminobutírico/fisiologiaRESUMO
The level of synaptotagmin I or II in developing spinal neurons was increased by injection of synaptotagmin messenger RNA into early blastomeres of Xenopus embryos. The effect of overexpression of synaptotagmin on synaptic function was assayed in Xenopus nerve-muscle cultures within two days after injection. At neuromuscular synapses made by synaptotagmin-overexpressing neurons, the frequency of miniature postsynaptic currents was markedly reduced, while their mean amplitude was unchanged, as compared to those of control neurons in the same culture. The amplitude of evoked postsynaptic currents elicited by low-frequency test stimuli was not affected by overexpression. However, synapses made by synaptotagmin-overexpressing neurons exhibited significantly higher paired-pulse facilitation and reduced tetanus-induced depression of the synaptic response, and there was also an increased number of synaptic vesicles at regions 100-300 nm from the plasmalemma at such synapses. These results show that synaptotagmins can exert an inhibitory action on the spontaneous exocytosis of synaptic vesicles. The effects on short-term plasticity suggest that synaptotagmin may facilitate vesicular supply for the evoked release during higher frequency transmission.
Assuntos
Proteínas de Ligação ao Cálcio/biossíntese , Glicoproteínas de Membrana/biossíntese , Proteínas do Tecido Nervoso/biossíntese , Junção Neuromuscular/fisiologia , Plasticidade Neuronal/fisiologia , Medula Espinal/embriologia , Medula Espinal/metabolismo , Sinapses/fisiologia , Animais , Células Cultivadas , Eletrofisiologia , Potenciais Evocados/fisiologia , Humanos , Imuno-Histoquímica , Microscopia Eletrônica , Junção Neuromuscular/ultraestrutura , RNA Mensageiro/biossíntese , Medula Espinal/ultraestrutura , Sinapses/ultraestrutura , Sinaptotagmina I , Sinaptotagminas , Transcrição Gênica , Xenopus laevisRESUMO
Retrograde signaling from the postsynaptic cell to the presynaptic neuron is essential for the development, maintenance, and activity-dependent modification of synaptic connections. This review covers various forms of retrograde interactions at developing and mature synapses. First, we discuss evidence for early retrograde inductive events during synaptogenesis and how maturation of presynaptic structure and function is affected by signals from the postsynaptic cell. Second, we review the evidence that retrograde interactions are involved in activity-dependent synapse competition and elimination in developing nervous systems and in long-term potentiation and depression at mature synapses. Third, we review evidence for various forms of retrograde signaling via membrane-permeant factors, secreted factors, and membrane-bound factors. Finally, we discuss the evidence and physiological implications of the long-range propagation of retrograde signals to the cell body and other parts of the presynaptic neuron.
Assuntos
Neurônios/fisiologia , Transdução de Sinais , Sinapses/fisiologia , Animais , Transporte Axonal , Citoesqueleto/fisiologia , Citosol/fisiologia , Humanos , Terminações Nervosas/fisiologia , Sistemas do Segundo MensageiroRESUMO
The hypothesis that synaptic functions can be regulated by neurotrophins secreted from the postsynaptic cell was examined in Xenopus nerve-muscle cultures. Neuromuscular synapses formed on myocytes overexpressing neurotrophin-4 (M+ synapses) exhibited a higher level of spontaneous synaptic activity and enhanced evoked synaptic transmission as compared to those formed on normal control myocytes (M- synapses). The NT-4 effects involve a potentiation of presynaptic transmitter secretion as well as a lengthening of the mean burst duration of postsynaptic low conductance acetylcholine channels. Repetitive stimulation of either the presynaptic neuron or the postsynaptic myocyte led to a potentiation of synaptic transmission at M+ synapses. All potentiation effects of NT-4 overexpression were abolished by the extracellular presence of TrkB-IgG but not by the presence of TrkA-IgG, indicating that postsynaptic secretion of NT-4 was responsible for the synaptic modification.
Assuntos
Músculos/fisiologia , Fatores de Crescimento Neural/fisiologia , Junção Neuromuscular/fisiologia , Neurônios/fisiologia , Sinapses/fisiologia , Transcrição Gênica , Animais , Células Cultivadas , Estimulação Elétrica , Embrião não Mamífero/fisiologia , Potenciais Evocados , Feminino , Fertilização in vitro , Proteínas de Fluorescência Verde , Proteínas Luminescentes/biossíntese , Masculino , Fatores de Crescimento Neural/biossíntese , Técnicas de Patch-Clamp , Proteínas Proto-Oncogênicas/fisiologia , RNA Mensageiro/metabolismo , Receptores Proteína Tirosina Quinases/fisiologia , Receptor do Fator Neutrófico Ciliar , Receptor trkA , Receptores de Fator de Crescimento Neural/fisiologia , Proteínas Recombinantes de Fusão/biossíntese , Transmissão Sináptica , XenopusRESUMO
Triple whole-cell recordings from simple networks of cultured hippocampal neurons show that Induction of long-term depression at glutamatergic synapses is accompanied by a back propagation of depression to Input synapses on the dendrite of the presynaptic neuron. The depression also propagates laterally to divergent outputs of the presynaptic neuron and to convergent inputs on the postsynaptic neuron. There is no forward propagation of depression to the output of the postsynaptic neuron and no presynaptic propagation accompanying long-term depression at GABAergic synapses. Activity-induced synaptic modification is therefore not restricted to the activated synapse, but selectively propagates throughout the neural network.
Assuntos
Rede Nervosa/fisiologia , Plasticidade Neuronal/fisiologia , Sinapses/fisiologia , Animais , Células Cultivadas , Ácido Glutâmico/metabolismo , Hipocampo/citologia , Hipocampo/fisiologia , Potenciais da Membrana , Inibição Neural , Ratos , Ácido gama-Aminobutírico/metabolismoRESUMO
Development of the nervous system depends on the correct pathfinding and target recognition by the growing tip of an axon, the growth cone. Diffusible or substrate-bound molecules present in the environment may serve as either attractants or repellents to influence the direction of growth-cone extension. Here we report that differences in cyclic-AMP-dependent activity in a neuron may result in opposite turning of the growth cone in response to the same guidance cue. A gradient of brain-derived neurotrophic factor normally triggers an attractive turning response of the growth cone of Xenopus spinal neurons in culture, but the same gradient induces repulsive turning of these growth cones in the presence of a competitive analogue of cAMP or of a specific inhibitor of protein kinase A. This cAMP-dependent switch of the turning response was also found for turning induced by acetylcholine, but not for the turning induced by neurotrophin-3 (NT-3). Thus, in the presence of other factors that modulate neuronal cAMP-dependent activity, the same guidance cue may trigger opposite turning behaviours of the growth cone during its pathfinding in the nervous system.
Assuntos
Axônios/fisiologia , AMP Cíclico/fisiologia , Animais , Fator Neurotrófico Derivado do Encéfalo/fisiologia , Cálcio/fisiologia , Diferenciação Celular/fisiologia , Movimento Celular/fisiologia , Células Cultivadas , Transdução de Sinais , Medula Espinal/citologia , XenopusRESUMO
Netrin-1 promotes outgrowth of axons in vitro through the receptor Deleted in Colorectal Cancer (DCC) and elicits turning of axons within embryonic explants when presented as a point source. It is not known whether netrin-1 alone can elicit turning nor whether DCC mediates the turning response. We show that Xenopus retinal ganglion cell growth cones orient rapidly toward a pipette ejecting netrin-1, an effect blocked by antibodies to DCC. In vitro, netrin-1 induces a complex growth cone morphology reminiscent of that at the optic nerve head, a site of netrin-1 expression in vivo. These results demonstrate that netrin-1 can function alone to induce turning, implicate DCC in this response, and support the idea that netrin-1 contributes to steering axons out of the retina.
Assuntos
Moléculas de Adesão Celular/fisiologia , Embrião não Mamífero/fisiologia , Fatores de Crescimento Neural/farmacologia , Fatores de Crescimento Neural/fisiologia , Receptores de Superfície Celular/fisiologia , Retina/embriologia , Células Ganglionares da Retina/fisiologia , Proteínas Supressoras de Tumor , Sequência de Aminoácidos , Animais , Anticorpos/farmacologia , Polaridade Celular , Receptor DCC , Regulação da Expressão Gênica no Desenvolvimento , Humanos , Dados de Sequência Molecular , Fatores de Crescimento Neural/biossíntese , Receptores de Netrina , Netrina-1 , Neuritos/efeitos dos fármacos , Neuritos/fisiologia , Neuritos/ultraestrutura , Nervo Óptico/embriologia , Técnicas de Cultura de Órgãos , Reação em Cadeia da Polimerase , Receptores de Superfície Celular/imunologia , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/efeitos dos fármacos , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Transcrição Gênica , XenopusRESUMO
Netrin-1 is known to function as a chemoattractant for several classes of developing axons and as a chemorepellent for other classes of axons, apparently dependent on the receptor type expressed by responsive cells. In culture, growth cones of embryonic Xenopus spinal neurons exhibited chemoattractive turning toward the source of netrin-1 but showed chemorepulsive responses in the presence of a competitive analog of cAMP or an inhibitor of protein kinase A. Both attractive and repulsive responses were abolished by depleting extracellular calcium and by adding a blocking antibody against the netrin-1 receptor Deleted in Colorectal Cancer. Thus, nerve growth cones may respond to the same guidance cue with opposite turning behavior, dependent on other coincident signals that set the level of cytosolic cAMP.
Assuntos
Quimiotaxia/fisiologia , AMP Cíclico/fisiologia , Fatores de Crescimento Neural/farmacologia , Neurônios/fisiologia , Medula Espinal/embriologia , Proteínas Supressoras de Tumor , Animais , Anticorpos/farmacologia , Moléculas de Adesão Celular/fisiologia , Células Cultivadas , Quimiotaxia/efeitos dos fármacos , Galinhas , AMP Cíclico/análogos & derivados , AMP Cíclico/metabolismo , AMP Cíclico/farmacologia , Embrião não Mamífero , Receptores de Netrina , Netrina-1 , Neurônios/citologia , Neurônios/efeitos dos fármacos , Receptores de Superfície Celular/fisiologia , Proteínas Recombinantes/farmacologia , Tionucleotídeos/farmacologia , XenopusRESUMO
Synapsins are a family of neuron-specific phosphoproteins that are localized within the presynaptic terminals in adult brain. Previous work has demonstrated that introduction of exogenous synapsins I(a + b) or IIa into Xenopus spinal neurons promoted maturation of the neuromuscular synapse in a nerve-muscle co-culture system. We have now studied the expression of endogenous Xenopus synapsin I during synaptic maturation in vivo and in culture, using a polyclonal antibody raised against Xenopus synapsin I. Immunoprecipitation experiments indicated that synapsin I was not detectable during the early phase of synaptogenesis in vivo, and exhibited a marked increase during the period of synaptic maturation. In contrast, the expression of synaptophysin, another synaptic vesicle protein, was detected at the start of nervous system formation, and remained at a high level thereafter. Similar expression profiles for the two proteins were also observed in immunocytochemical studies of Xenopus spinal neurons in culture: intense staining of synaptophysin was found on the first day, while synapsin I was not detected until after three days in culture. The expression of synapsin I correlated very well with the appearance of a bell-shaped amplitude distribution of spontaneous synaptic currents, a physiological parameter which reflects functional maturation of the neuromuscular synapse. In one-day-old cultures grown in the absence of laminin, an extracellular matrix protein known to be present at the neuromuscular junction, the amplitude distribution of virtually all synapses was skewed towards smaller values. In contrast, when laminin was used as a culture substrate, many synapses exhibited a bell-shaped amplitude distribution. Laminin treatment also induced synapsin I expression in one-day-old cultures. These results suggest that the expression of endogenous synapsin I may regulate maturation at neuromuscular synapses.
Assuntos
Desenvolvimento Muscular , Músculo Esquelético/crescimento & desenvolvimento , Junção Neuromuscular/fisiologia , Sinapses/fisiologia , Sinapsinas/biossíntese , Animais , Western Blotting , Células Cultivadas , Técnicas de Cocultura , Eletrofisiologia , Imuno-Histoquímica , Laminina/farmacologia , Potenciais da Membrana/fisiologia , Músculo Esquelético/inervação , Músculo Esquelético/fisiologia , Técnicas de Patch-Clamp , Testes de Precipitina , Sinapses/metabolismo , Sinaptofisina/biossíntese , Xenopus laevisRESUMO
A diversity of neurotrophic factors are required for the differentiation and survival of neurons and for maintaining their phenotype. By virtue of the rapid time scale of signal transduction in the cytosol, many of these factors also acutely regulate neuronal functions as diverse as synaptic transmission and nerve growth. These fast actions greatly expand the regulatory role of neurotrophic factors, particularly in the synaptic plasticity of developing nervous systems.
Assuntos
Fatores de Crescimento Neural/fisiologia , Animais , Potenciação de Longa Duração , Fatores de Crescimento Neural/farmacologia , Neurônios/citologia , Neurônios/efeitos dos fármacos , Neurotransmissores/metabolismo , Terminações Pré-Sinápticas/fisiologia , Sinapses/fisiologia , Fatores de TempoRESUMO
Extracellular application of brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) to developing neuromuscular junctions in Xenopus nerve-muscle cultures resulted in an increase in the frequency of spontaneous synaptic currents (SSCs) and in the amplitude of nerve-evoked synaptic currents. Analyses of the amplitude and time course of the SSCs suggest that these effects are attributable to elevation of presynaptic transmitter release. The actions of these two factors on the transmitter secretion process, however, are distinctly different. Fura-2 Ca2+ imaging showed that an increase in presynaptic cytosolic Ca2+ ([Ca2+]i) accompanied the synaptic potentiation by BDNF, whereas no change in [Ca2+]i was observed during synaptic potentiation by CNTF. Removing external Ca2+ also abolished the potentiating effect of BDNF but did not influence the CNTF effect. Moreover, the two factors exerted different effects on the short-term synaptic plasticity. Paired-pulse facilitation normally found at these synapses was reduced by BDNF but unaffected by CNTF; CNTF, but not BDNF, reduced the extent of synaptic depression during high-frequency tetanic stimulation. Finally, the potentiation effect of BDNF and CNTF on spontaneous transmitter release was additive when both factors were applied together to the synapse at saturating concentrations (100 ng/ml) and was highly synergistic when low doses (1 and 10 ng/ml) of both factors were used. These results suggest that because of their differential effects on the secretory machinery, BDNF and CNTF may act cooperatively in modulating the development and functioning of synapses.