RESUMO
The LIM and SH3 protein 1 (LASP1) is a focal adhesion protein. Its expression is increased in many malignant tumors. However, little is known about the physiological role of the protein. In the present study, we investigated the expression and function of LASP1 in normal skin, melanocytic nevi and malignant melanoma. In normal skin, a distinct LASP1 expression is visible only in the basal epidermal layer while in nevi LASP1 protein is detected in all melanocytes. Melanoma exhibit no increase in LASP1 mRNA compared to normal skin. In melanocytes, the protein is bound to dynamin and mainly localized at late melanosomes along the edges and at the tips of the cell. Knockdown of LASP1 results in increased melanin concentration in the cells. Collectively, we identified LASP1 as a hitherto unknown protein in melanocytes and as novel partner of dynamin in the physiological process of membrane constriction and melanosome vesicle release.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Proteínas do Citoesqueleto/metabolismo , Proteínas com Domínio LIM/metabolismo , Melaninas/metabolismo , Melanócitos/metabolismo , Melanoma/metabolismo , Nevo Pigmentado/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/genética , Adesão Celular , Movimento Celular , Proliferação de Células , Células Cultivadas , Proteínas do Citoesqueleto/genética , Dinaminas/metabolismo , Técnicas de Silenciamento de Genes , Humanos , Proteínas com Domínio LIM/genética , Melanoma/genética , Nevo Pigmentado/genéticaRESUMO
Primary cutaneous T-cell lymphomas mostly occur in patients of middle and higher age. Their rarity and an oftentimes atypical clinical presentation in childhood as well as the reluctance of taking biopsies in children are reasons for a delayed diagnosis. We report the case of an 11-year-old boy with a 7-year history of slowly progressive CD8+CD56+ mycosis fungoides of the cytotoxic immunophenotype. His trunk and extremities were affected by extensive pale-erythematous patches and plaques with fine scaling. In addition, several poikilodermatous lesions were present on his thighs. Improvement was achieved by topical mometasone furoate treatment. On the basis of our observation, a brief review on cutaneous T-cell lymphomas in childhood and on CD8+ subtypes in particular is given. Clinicopathological correlation is crucial for establishing the correct diagnosis and for estimation of the prognosis.
Assuntos
Antígeno CD56/imunologia , Linfócitos T CD8-Positivos/imunologia , Micose Fungoide/patologia , Neoplasias Cutâneas/patologia , Biópsia , Criança , Fármacos Dermatológicos/uso terapêutico , Humanos , Imunofenotipagem , Linfoma Cutâneo de Células T/imunologia , Linfoma Cutâneo de Células T/patologia , Masculino , Furoato de Mometasona/uso terapêutico , Micose Fungoide/diagnóstico por imagem , Micose Fungoide/tratamento farmacológico , Micose Fungoide/imunologia , Neoplasias Cutâneas/tratamento farmacológico , Neoplasias Cutâneas/imunologia , UltrassonografiaAssuntos
Fissura Anal/etiologia , Histiocitose de Células de Langerhans/complicações , Histiocitose de Células de Langerhans/diagnóstico , Idoso , Anti-Inflamatórios/uso terapêutico , Antineoplásicos Fitogênicos/uso terapêutico , Histiocitose de Células de Langerhans/tratamento farmacológico , Humanos , Masculino , Pessoa de Meia-Idade , Prednisona/uso terapêutico , Vimblastina/uso terapêuticoRESUMO
BACKGROUND: Disseminated granuloma annulare (DGA) is a benign and usually asymptomatic skin disease. However, many patients feel aesthetically disfigured and ask for treatment. Until today, no standard therapy is recommended. OBJECTIVE: To evaluate the safety and efficacy of oral vitamin E treatment compared to the natural course of DGA. METHODS: This single-centre observational cohort study included 38 consecutive patients with histologically confirmed DGA. 21 patients underwent treatment with oral vitamin E, whereas 17 patients preferred a wait-and-see approach. RESULTS: Complete healing (40%) and improvement (30%) were frequently seen under oral vitamin E therapy. However, DGA also spontaneously disappeared in 31% and improved in 25% of untreated control patients. Vitamin E therapy was very well tolerated. CONCLUSIONS: Oral vitamin E treatment is a safe and probably effective therapy for DGA. As the natural course of DGA leads to complete healing or significant improvement in many cases, 'primum nil nocere' should be the maxim.
Assuntos
Granuloma Anular/tratamento farmacológico , Vitamina E/uso terapêutico , Vitaminas/uso terapêutico , Administração Oral , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Remissão Espontânea , Estudos Retrospectivos , Conduta Expectante , Adulto JovemRESUMO
We describe a woman presenting primarily with slowly progressing scarring alopecia. Course, symptoms, and clinical picture were highly suggestive for lichen planus. But mycological investigations revealed that cicatricial alopecia was caused by a specific infection with Trichophyton schoenleinii running a chronic course with minimal skin inflammation.
Assuntos
Alopecia/microbiologia , Líquen Plano , Tinha Favosa/diagnóstico , Trichophyton/isolamento & purificação , Adulto , Alopecia/tratamento farmacológico , Alopecia/patologia , Antifúngicos/uso terapêutico , Feminino , Doenças do Cabelo/diagnóstico , Doenças do Cabelo/tratamento farmacológico , Doenças do Cabelo/microbiologia , Folículo Piloso/microbiologia , Humanos , Esporos Fúngicos/isolamento & purificação , Tinha Favosa/tratamento farmacológico , Tinha Favosa/microbiologiaRESUMO
BACKGROUND: There are myriads of potentially irritant agents causing acute irritant contact dermatitis. In the large majority of cases, dermatitis is mild to moderate, and patients do not need hospitalization. However, some agents or special circumstances may cause severe dermatitis requiring more intensive therapy. OBJECTIVES: The aim of this study was to evaluate causative agents of severe acute irritant contact dermatitis requiring hospitalization. METHODS: In this single-centre observational cohort study, we included 54 consecutive patients presenting with signs and symptoms of acute irritant contact dermatitis for which hospitalization was necessary. The severity of dermatitis was graded (grade I-IV) according to intensity, and details related to the skin irritation (irritant agent, area of exposure, time interval to onset of symptoms, and duration of hospitalization) were determined. RESULTS: All cases with severe ulcerative dermatitis (grade IV) were caused by wet cement, owing to prolonged skin contact. These cement burns are clearly associated with amateur work, younger age, male preponderance, and leg localization. CONCLUSIONS: The study data provide clear-cut evidence that wet cement is a severely irritant substance that regularly causes the most severe form of acute irritant contact dermatitis. The main causative prerequisite for these cement burns is do-it-yourself work with poor protective measures.