RESUMO
AIM: To identify the association between the expression of lncRNA NEAT1 and clinicopathological characteristics of patients with HCC, and to explore the prognostic significance of lncRNA NEAT1 in predicting prognosis of HCC. METHODS: We retrospectively reviewed 86 patients with HCC (35 female, 51 male) managed in our institution between 2009 and 2014. The expression level of lncRNA NEAT1 was detected by real-time PCR. Prognostic factors were evaluated using Kaplan-Meier curves and Cox proportional hazards models. RESULTS: For the entire cohort of 86 patients, we showed that the expression level of NEAT1 was significantly higher in HCC tissues compared with non-tumorous tissues and NEAT1 was increased obviously in the HCC cell lines including SMMC-7721, Huh-7 and Hep3B (P < 0.001). MTT assay showed that si-NEAT1 remarkably inhibited the cell proliferation in three HCC cell lines. Moreover, over-expression of lncRNA NEAT1 was closely related to liver cirrhosis (P = 0.026), microvascular invasion (MVI) (P = 0.023), and TNM stage (P = 0.017). After adjusting for competing risk factors, we identified that expression level of lncRNA NEAT1 was an independently risk factor associated with the prognosis of patients with HCC (P = 0.031). CONCLUSIONS: In this study, we found NEAT1 expressed significantly higher in HCC tissues compared with non-tumorous tissues. Overexpression of lncRNA NEAT1 was an independently risk factor associated with the prognosis of patients with HCC.
Assuntos
Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/cirurgia , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/cirurgia , RNA Longo não Codificante/genética , Adulto , Idoso , Vasos Sanguíneos/patologia , Linhagem Celular Tumoral , Proliferação de Células/genética , China , Feminino , Expressão Gênica , Hepatectomia , Humanos , Cirrose Hepática/genética , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Estadiamento de Neoplasias , Prognóstico , RNA Interferente Pequeno/genética , Estudos Retrospectivos , Fatores de Risco , Taxa de SobrevidaRESUMO
The effect and experience in treating 200 patients having gallbladder stones with extracorporeal shock wave lithotripsy (ESWL) were reported. Traditional Chinese medicine was used in combination in these patients. The overall effective lithotriptic rate was 91%. In a follow-up study of 137 of the cases, the rate of complete disappearance of gallbladder stones was 16.8%, 25.5% and 39.4%, respectively 1, 3, and 6 months after lithotripsy. During treatment, the patients had no remarkable discomfort. The authors consider that the lithotriptic effect is related to the nature, size, morphology and structure of the stones and ESWL is promising non-operative treatment. However, improved instrumentation is necessary for better efficacy of lithotripsy.
Assuntos
Colelitíase/terapia , Litotripsia/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Colelitíase/diagnóstico por imagem , Terapia Combinada , Medicamentos de Ervas Chinesas/uso terapêutico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , UltrassonografiaRESUMO
52 patients with chronic gastritis infected by campylobacter pyloridis (CP) were selected for random observation. The course of treatment was 4 weeks. In the group treated with granule-gastritis, the effective rate for CP was 81.3% (26/32), and the improvement rates of the symptoms and histopathological lesion were 87.5% (28/32) and 40.6% (13/32) respectively. In the group treated with Wei-Mei-Su as the control group, the figures were 25%, 40% and 10% respectively. These differences were statistically significant (P less than 0.01-0.000). The results showed that the effect of granule-gastritis on chronic gastritis infected with CP was better than that of Wei-Mei-Su.