In vivo study of bovine hydroxyapatite-gelatin-hydroxypropyl methylcellulose with alendronate as injectable bone substitute composite in osteoporotic animal model.

The injectable bone substitute (IBS) is a self-setting local drug delivery system that adjusts the shape of the bone gap in the fracture. This study aimed to examine the effectiveness of IBS composites of bovine hydroxyapatite (BHA) and alendronate (Ale) in accelerating bone growth in osteoporotic rats. IBS was made by mixing BHA with gelatin 5%, hydroxypropyl methylcellulose (HPMC) 2%, and Ale 10%. The physical properties of IBS were viscosity, injectability, and density tests. Twenty-four female Wistar rats were divided into four groups. After 8 weeks, 2 mm gap was made in the right femur of all rats and filled with IBS. The healing process was observed after 6 weeks with X-ray imaging and H and E staining. The obtained results showed viscosity, injectability, and density value of IBS from 30.4 to 39.4 dPa.s, 98.22%-98.64%, and 0.6325-0.8409 g/cm3, respectively. X-ray imaging and histology results proved the condition of osteoporosis in rats with ovariectomy. The addition of BHA-Gel-HPMC-Ale significantly affected the number of osteoblasts, osteocytes, and osteoclasts (P < 0.05). After 45 days of observation, the addition of BHA-Gel-HPMC-Ale showed the highest mean number of osteoblasts, osteocytes, and osteoclasts, which were 25.00 ± 3.00, 64.33 ± 11.15, and 5.67 ± 0.58 compared to BHA-Gel-HPMC and positive control groups. The BHA-Gel-HPMC-Ale IBS has the potential to reverse osteoporosis. Nevertheless, the underlying potential of these biomaterials to reverse osteoporosis needs further research.

Electrodermal Activity for Measuring Cognitive and Emotional Stress Level.

Stress can lead to harmful conditions in the body, such as anxiety disorders and depression. One of the promising noninvasive methods, which has been widely used in detecting stress and emotion, is electrodermal activity (EDA). EDA has a tonic and phasic component called skin conductance level and skin conductance response (SCR). However, the components of the EDA cannot be directly extracted and need to be deconvolved to obtain it. The EDA signals were collected from 18 healthy subjects that underwent three sessions - Stroop test with increasing stress levels. The EDA signals were then deconvoluted by using continuous deconvolution analysis (CDA) and convex optimization approach to electrodermal activity (cvxEDA). Four features from the result of the deconvolution process were collected, namely sample average, standard deviation, first absolute difference, and normalized first absolute difference. Those features were used as the input of the classification process using the extreme learning machine (ELM). The output of classification was the stress level; mild, moderate, and severe. The visual of the phasic component using cvxEDA is more precise or smoother than the CDA's result. However, both methods could separate SCR from the original skin conductivity raw and indicate the small peaks from the SCR. The classification process results showed that both CDA and cvxEDA methods with 50 hidden layers in ELM had a high accuracy in classifying the stress level, which was 95.56% and 94.45%, respectively. This study developed a stress level classification method using ELM and the statistical features of SCR. The result showed that EDA could classify the stress level with over 94% accuracy. This system could help people monitor their mental health during overworking, leading to anxiety and depression because of untreated stress.

The combined effect of magnetic and electric fields using on/off infrared light on the blood sugar level and the diameter of Langerhans islets of diabetic mice.

BACKGROUND AND AIM: At present, diabetes is treated with oral antidiabetic medicines, such as sulfonylureas and thiazolidine, as well as insulin injection. However, these methods have several shortcomings. Therefore, alternatives for treating diabetes mellitus (DM) are needed. This study aims to determine the combined effect of magnetic and electric fields on blood sugar levels and the diameter of Langerhans islets of diabetic mice. MATERIALS AND METHODS: Induction of DM in mice was carried out by administering lard for 2 weeks and continued with an intraperitoneal injection of streptozotocin, dissolved in a 4.5 pH citrate buffer, and administered in a dose of 30 mg/kg bodyweight for 5 days. Treatments were used in combination with magnetic and electric fields using on/off infrared light. Blood samples were pipetted through the tip of mice's tails to establish the blood sugar level for each individual mouse. Histology preparation of the pancreas organ was affected using the histology standard as well as hematoxylin and eosin staining methods. Langerhans islet diameter data were analyzed using analysis of variance followed by Duncan's multiple range test. Data analysis was performed at ssssssss=0.05. RESULTS: The results showed that the combined treatment of permanent magnetic and unidirectional electric fields (PS) caused changes in blood sugar levels that were not significantly different from the normal control group. The PS treatment improved the diameter of the Langerhans islets but not to a significant degree compared to other treatments. CONCLUSION: The use of PS treatment is effective for reducing the blood sugar levels of diabetic mice and improving the diameter of their Langerhans islets.

The effect of electric field, magnetic field, and infrared ray combination to reduce HOMA-IR index and GLUT 4 in diabetic model of Mus musculus.

Diabetes mellitus (DM) is a metabolic disorder characterized by high blood glucose level (hyperglycemia). Type 2 diabetes mellitus is mainly featured by low cell sensitivity towards insulin stimulation, caused by ectopic fat storage. Insulin resistance can be quantified from high number of HOMA-IR index and observed from glucose transporter 4 (GLUT-4) translocation on membrane of skeletal muscle cells. Combined treatment of electric field, magnetic field, and infrared ray have potential to reduce insulin resistance due to improving blood circulation and increasing intracellular Ca2+ level. The aim of study was to determine the effect of electric field, magnetic field, and infrared ray combination to lower insulin resistance in the type II diabetic model of Mus musculus. This study used 30 adult male mice strain BALB/c. Diabetes was induced using high-fat diet/streptozotocin method until random blood glucose level reached > 200 mg/dL. Diabetic mice were then exposed to electrical field (static and dynamic), magnetic field (static and induce), and infrared ray (with or without infrared ray) combination therapy 15 min daily for 28 days. Fasting blood glucose level, plasma insulin level, HOMA-IR index, and membrane GLUT-4 density after treatment were analyzed statistically at α = 0.05. Result showed that exposure combination of electrical field, magnetic field, and infrared were found to be able to lower fasting blood glucose level and HOMA-IR index significantly, but plasma insulin level and GLUT-4 density were not found to be significantly different compared to diabetic control. Based on current study result, the best combination for reducing insulin resistance in diabetic mice is BsEsI (combination of static magnetic field (Bs), static electric field (Es), with infrared (I)), indicated by lowest HOMA-IR compared to other groups. Exposure to combination of magnetic field, electrical field, and infrared resulted in lowering fasting blood glucose level and HOMA-IR index in diabetic mice, indicating reduced insulin resistance.

The effects of laser diode treatment on liver dysfunction of Mus musculus due to carbofuran exposure: An in vivo study.

OBJECTIVE: The aim of this study is to determine the effect of laser diode as an alternative treatment on liver dysfunction (in vivo study) that is caused by carbofuran using male mice (Mus musculus) strain Balb/C. MATERIALS AND METHODS: The samples were divided into three groups, namely, Group C-L- (control group, no treatment), Group C+L- (only treated by carbofuran treatment), and Group C+L+ (treatment group, treated by carbofuran and laser-puncture) with five replications each. After being treated, each liver slice of samples was observed using microscope to get the histology result and then scored. RESULTS: Carbofuran contamination can lead to inflammation of cells and necrosis. The histology results and the scoring test showed that the liver cells repair with the energy dose of laser diode at 0.5 and 1.0 Joule. CONCLUSION: The optimum energy dose in this study was 1.0 Joule which had the closest score of inflammatory cells and necrosis to normal liver cells.

Gas Sensor Array System Properties for Detecting Bacterial Biofilms.

BACKGROUND: Gas sensor array system is a device that mimics the work of how the nose smells using the gas sensors that could give response toward specific odors. It is used for characterizing the different blended gas that is suited with the biological working nose principle. Thus, it could be used to detect the dental and oral diseases. Periodontitis is one of the diseases caused by the damage on the teeth due to the chronic infection on the gingival structure marked with bacterial plaque and calculus. This study aims to develop an electric nose for odor detection application on the periodontal bacterial biofilm as early detection device for dental and oral disease. METHODS: This device is designed as a portable device to ease the data acquisition. The measured data were stored at a database system connected to a real-time computer. A gas array sensor system with six gas sensors (TGS 826, TGS 2602, TGS 2600, TGS 2611, TGS 2612, and TGS 2620) has been assembled for the early detection application for dental and oral disease excreted by the bacterial biofilm that caused dental and oral disease, including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus mutans, and Enterococcus faecalis. RESULTS: TGS 826 and TGS 2602 sensor had the best response showed by the high ADC delta value. CONCLUSION: GS 826 and TGS 2602 sensor could be used as a candidate for early detection device for dental and oral disease.