Molecular spectra and docking simulations investigated the binding mechanisms of tetracycline onto E. coli extracellular polymeric substances.

Extracellular polymeric substances (EPS), which were an important fraction of natural organic matter (NOM), played an important role in various environmental processes. However, the heterogeneity, complexity, and dynamics of EPS make their interactions with antibiotics elusive. Using advanced multispectral technology, this study examined how EPS interacts with different concentrations of tetracycline (TC) in the soil system. Our results demonstrated that protein-like (C1), fulvic-like (C2), and humic-like (C3) fractions were identified from EPS. Two-dimensional synchronous correlation spectroscopy (2D-SF-COS) indicated that the protein-like fraction gave faster responses than the fulvic-like fraction during the TC binding process. The sequence of structural changes in EPS due to TC binding was revealed by two-dimensional Fourier Transformation Infrared correlation spectroscopy (2D-FTIR-COS) as follows: 1550 > 1660 > 1395 > 1240 > 1087 cm-1. It is noteworthy that the sensitivity of the amide group to TC has been preserved, with its intensity gradually increasing to become the primary binding site for TC. The integration of hetero-2DCOS maps with moving window 2D correlation spectroscopy (MW2DCOS) provided a unique insight into understanding the correlation between EPS fractions and functional groups during the TC binding process. Moreover, molecular docking (MD) discovered that the extracellular proteins would provide plenty of binding sites with TC through salt bridges, hydrogen bonds, and π-π base-stacking forces. With these results, systematic investigations of the dynamic changes in EPS components under different concentrations of antibiotic exposure demonstrated the advanced capabilities of multispectral technology in examining intricate interactions with EPS in the soil environment.

Vacancy Engineering in 2D Transition Metal Chalcogenide Photocatalyst: Structure Modulation, Function and Synergy Application.

Transition metal chalcogenides (TMCs) are widely used in photocatalytic fields such as hydrogen evolution, nitrogen fixation, and pollutant degradation due to their suitable bandgaps, tunable electronic and optical properties, and strong reducing ability. The unique 2D malleability structure provides a pre-designed platform for customizable structures. The introduction of vacancy engineering makes up for the shortcomings of photocorrosion and limited light response and provides the greatest support for TMCs in terms of kinetics and thermodynamics in photocatalysis. This work reviews the effect of vacancy engineering on photocatalytic performance based on 2D semiconductor TMCs. The characteristics of vacancy introduction strategies are summarized, and the development of photocatalysis of vacancy engineering TMCs materials in energy conversion, degradation, and biological applications is reviewed. The contribution of vacancies in the optical range and charge transfer kinetics is also discussed from the perspective of structure manipulation. Vacancy engineering not only controls and optimizes the structure of the TMCs, but also improves the optical properties, charge transfer, and surface properties. The synergies between TMCs vacancy engineering and atomic doping, other vacancies, and heterojunction composite techniques are discussed in detail, followed by a summary of current trends and potential for expansion.

Feature engineering for improved machine-learning-aided studying heavy metal adsorption on biochar.

Due to the broad interest in using biochar from biomass pyrolysis for the adsorption of heavy metals (HMs) in wastewater, machine learning (ML) has recently been adopted by many researchers to predict the adsorption capacity (η) of HMs on biochar. However, previous studies focused mainly on developing different ML algorithms to increase predictive performance, and no study shed light on engineering features to enhance predictive performance and improve model interpretability and generalizability. Here, based on a dataset widely used in previous ML studies, features of biochar were engineered-elemental compositions of biochar were calculated on mole basis-to improve predictive performance, achieving test R2 of 0.997 for the gradient boosting regression (GBR) model. The elemental ratio feature (H-O-2N)/C, representing the H site links to C (non-active site to HMs), was proposed for the first time to help interpret the GBR model. The (H-O-2N)/C and pH of biochar played essential roles in replacing cation exchange capacity (CEC) for predicting η. Moreover, expanding the coverages of variables by adding cases from references improved the generalizability of the model, and further validation using cases without CEC and specific surface area (R2 0.78) and adsorption experimental results (R2 0.72) proved the ML model desirable. Future studies in this area may take into account algorithm innovation, better description of variables, and higher coverage of variables to further increase the model's generalizability.

Sonochemically assisted the synthesis and catalytic application of bismuth-based photocatalyst: A mini review.

Recently, bismuth (Bi)-based photocatalysts have been a well-deserved hotspot in the field of photocatalysis owning to their photoelectrochemical properties driven by the distortion of the Bi 6 s orbital, while their narrow band gap and poor quantum efficiency still restrict their application. With the development of ultrasonic technology, it is expected to become a broom to clear the application obstacles of Bi-based photocatalysts. The special forces and environmental conditions brought by ultrasonic irradiation play beneficial roles in the preparation, modification and performance releasement of Bi-based photocatalysts. In this review, the role and influencing factors of ultrasound in the preparation and modification of Bi-based photocatalysts were introduced. Crucially, the mechanism of the improving the performance for various types of Bi-based photocatalysts by ultrasound in the whole process of photocatalysis was deeply analyzed. Then, the application of ultrasonic synergistic Bi-based photocatalysts in contaminants treatment and energy conversion was briefly introduced. Finally, based on an unambiguous understanding of ultrasonic technology in assisting Bi-based photocatalysts, the future directions and possibilities for ultrasonic synergistic Bi-based photocatalysts are explored.

Effect of the Combination of Phosphate-Solubilizing Bacteria with Orange Residue-Based Activator on the Phytoremediation of Cadmium by Ryegrass.

Amendments with activators or microorganisms to enhance phytoremediation in toxic-metal-polluted soils have been widely studied. In this research, the production of indoleacetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase by phosphate-solubilizing bacteria was investigated during a pure culture experiment. Pot experiments were performed using Cd-polluted soil with the following treatments: control (CK, only ultrapure water), orange-peel-based activator (OG), and a combination of phosphate-solubilizing bacteria (Acinetobacter pitti) and OG (APOG). Ryegrass plant height and fresh weight, Cd content in ryegrass, total and available Cd soil content, soil enzyme activity, and soil bacterial diversity were determined in this work. The findings showed that the height of ryegrass in OG and APOG increased by 14.78% and 21.23%. In the APOG group, a decreased ratio of Cd was 3.37 times that of CK, and the bioconcentration factor was 1.28 times that of CK. The neutral phosphatase activity of APOG was 1.33 times that of CK and catalase activity was 1.95 times that of CK. The activity of urease was increased by 35.48%. APOG increased the abundance of beneficial bacteria and Proteobacteria was the dominant bacterium, accounting for 57.38% in APOG. Redundancy analysis (RDA) showed that nutrient elements were conducive to the propagation of the dominant bacteria, the secretion of enzymes, and the extraction rate of Cd in the soil. The possible enhancement mechanism of phytoremediation of cadmium by A. pitti combined with OG was that, on the one hand, APOG increased soil nutrient elements and enzyme activities promoted the growth of ryegrass. On the other hand, APOG activated Cd and boosted the movement of Cd from soil to ryegrass. This research offers insight for the combination of phosphate-solubilizing bacteria with an orange-peel-based activator to improve phytoremediation of Cd-contaminated soils and also provides a new way for the resource utilization of fruit residue.

Design strategies and mechanisms of g-C3N4-based photoanodes for photoelectrocatalytic degradation of organic pollutants in water.

Emerging photoelectrocatalytic (PEC) systems integrate the advantages of photocatalysis and electrocatalysis and are considered as a promising technology for solving the global organic pollution problem in water environments. Among the photoelectrocatalytic materials applied for organic pollutant degradation, graphitic carbon nitride (CN) has the combined advantages of environmental compatibility, stability, low cost, and visible light response. However, pristine CN has disadvantages such as low specific surface area, low electrical conductivity, and high charge complexation rate, and how to improve the degradation efficiency of PEC reaction and the mineralization rate of organic matter is the main problem faced in this field. Therefore, this paper reviews the progress of various functionalized CN used for PEC reaction in recent years, and the degradation efficiency of these CN-based materials is critically evaluated. First, the basic principles of PEC degradation of organic pollutants are outlined. Then, engineering strategies to enhance the PEC activity of CN (including morphology control, elemental doping, and heterojunction construction) are focused on, and the structure-activity relationships between these engineering strategies and PEC activity are discussed. In addition, the important role of influencing factors on the PEC system is summarized in terms of mechanism, to provide guidance for the subsequent research. Finally, suggestions and perspectives are provided for the preparation of efficient and stable CN-based photoelectrocatalysts for practical wastewater treatment applications.

Micro- and nano-plastics pollution and its potential remediation pathway by phytoremediation.

MAIN CONCLUSION: This review proposed that phytoremediation could be applied for the decontamination of MPs/NPs. Micro- and nano-plastics (MPs < 5 mm; NPs < 100 nm) are emerging contaminants. Much of the recent concerns have focused on the investigation of their pollution and their potential eco-toxicity. Yet little review was available on the decontamination of MPs/NPs. Recently, the uptake of MPs/NPs by plants has been confirmed. Here, in view of the current knowledge, this review introduces MPs/NPs pollution and highlights the updated information about the interaction between MPs/NPs and plants. This review proposed that phytoremediation could be a potential possible way for the in situ remediation of MPs/NPs-contaminated environment. The possible mechanisms, influencing factors, and existing problems are summarized, and further research needs are proposed. This review herein provides new insights into the development of plant-based process for emerging pollutants decontamination, as well as the alleviation of MPs/NPs-induced toxicity to the ecosystem.

Effect of different amounts of fruit peel-based activator combined with phosphate-solubilizing bacteria on enhancing phytoextraction of Cd from farmland soil by ryegrass.

To improve the uptake of heavy metals by plants and increase the effectiveness of phytoextraction, chelating agents are employed to change the speciation of heavy metals in soil and increase their bioavailability. However, the effect of a single activator is limited. In recent years, compound activators have been applied widely to improve phytoextraction efficiency. In this study, a fruit peel-based activator (OG) was prepared, containing a mixture of orange peel extracts and tetrasodium glutamate diacetate (GLDA) (1.6% v/v) in a ratio of 1:1 (v/v). The pot experiment was used to investigate the effects of different amounts of OG combined with phosphate-solubilizing bacteria (Acinetobacter pitti, AP) on the extraction of Cd from farmland soil by ryegrass (Lolium perenne L). The results indicated that the addition of OG and AP increased the pH and EC of the soil and improved the content of nutrient elements in the soil. The optimal combination of the application rates of OG and AP improved the growth of ryegrass and enhanced the phytoextraction of Cd. Redundancy analysis (RDA) showed that total soil nitrogen had the greatest influence on phytoextraction, with a contribution rate of 85.3%, followed by pH, with a contribution rate of 7.7%. Total nitrogen, pH, available phosphorus, alkaline nitrogen, and total organic matter were correlated positively with plant Cd, soil Cd decrease ratio, and the bioaccumulation factor but negatively with total Cd and available Cd. Based on the findings of this study, it is feasible to apply the fruit peel-based activator (amended with GLDA) and phosphate-solubilizing bacteria to enhance phytoextraction of Cd, which will provide a valuable reference for the treatment of heavy metal-contaminated soils and the reutilization of fruit peel waste. When applying the compound activator, it is recommended to consider the influence of the additional amount of compound activator on the extraction efficiency.

Expanding the valorization of waste mushroom substrates in agricultural production: progress and challenges.

Waste mushroom substrate (WMS) generated in large quantities from mushroom production process has caused severe environmental pollution. As a sustainable resource, the valorization of WMS in the agricultural field has attracted attention due to the abundant active components. A comprehensive review of valorization of WMS in agricultural production is meaningful to promote the further utilization of this resource. This paper provided an overview of the valorization in sustainable agricultural production using WMS, including animal and crop farming improvement, and agricultural environmental restoration. Moreover, the limitations and the possible development directions of WMS in agricultural production were discussed. Different sustainable cycle models for WMS in agricultural production were proposed. The aim of this review is to provide a feasible solution for the favorable treatment of WMS and improvement of agricultural production quality.

Extraction and utilization of active substances from edible fungi substrate and residue: A review.

The expansion of the edible fungi industry has resulted in the production of large amounts of edible fungus residues, causing great pressure on environmental protection.Therefore, research on edible fungus residue utilization has become a controversial issue. Thus far, numerous efforts have been devoted to separate active substances from edible fungus substrates and residues for high application value utilization. Building upon this, the main methods for extracting active substances from edible mushroom residues are reviewed, and the mechanisms, influencing factors, and trade-offs of the various methods are analysed. Furthermore, the existing and possible directions of utilization of the extracted active substances are reviewed and discussed. Finally, challenges and prospects for the extraction and utilization of different substances in edible fungus residues are proposed. This review provides an effective strategy for protecting the ecological environment and promoting the sustainable development of human society.

Introduction of acid mine drainage in the direct production of 5-hydroxymethylfurfural from raw biomass and expanding the use of biomass conversion residue.

Direct production of 5-hydroxymethylfurfural (HMF) through biomass always needs the addition of exogenous catalysts and causes extra costs. Herein, acid mine drainage (AMD), one of the traditional wastewaters, was introduced as a natural catalyst to produce HMF directly from lignocellulosic biomass. Key factors in the biomass conversion were optimized and investigated by the response surface methodology (RSM), and the HMF yield reached 13.51 wt% under optimal conditions. The metal elements and the acidic environment in AMD activated the Fenton reaction to effectively destroy the lignocellulose structure and synergistically promote the formation of HMF. Furthermore, the biomass substrate in the biomass conversion was indirectly modified by the AMD during this process. The biomass conversion residue could be prepared by pyrolysis to obtain a functional metal-loaded carbon material with good adsorption of thiamethoxam (THX), which provides a sustainable solution for the disposal of biomass conversion residue.

Mercury accumulation response of rice plant (Oryza sativa L.) to elevated atmospheric mercury and carbon dioxide.

New observations and updated models now suggest terrestrial ecosystems are net sink of atmospheric mercury (Hg), and the critical constrained process to identify the strengths of terrestrial sink is whether the large amount of Hg stored in vegetation originates from the soil as well as from the atmosphere. In this study, field open top chambers (OTCs) experiments reveal that rice plant can assimilate gaseous elemental mercury (GEM, Hg0) from the atmosphere through stomata, and Hg concentrations in rice leaves, upper and bottom stalks and grains increased with Hg0 levels in air, showing significantly quadratic linear relationships. Coupling field stable isotope soil amendment experiments, atmospheric source of Hg in rice plant is quantified with more than 90% of Hg accumulation in rice aboveground biomass from air and approximately 80% of rice root Hg from soil. Furthermore, elevated atmospheric carbon dioxide (CO2) exposure led to lower Hg concentration in rice tissues through reduction stomatal conductance of rice leaf, and subsequently impact the capacity of Hg storage in rice aboveground parts from the atmosphere. The findings from experiments provide a foundation for future quantification of atmospheric sink of crops in local and larger scales and comprehensive evaluation atmosphere - terrestrial processes and exposure risks in the global Hg cycling.

Hydrogen sulfide and calcium effects on cadmium removal and resistance in the white-rot fungus Phanerochaete chrysosporium.

Hydrogen sulfide (H2S), an emerging gas transmitter, has been shown to be involved in multiple intracellular physiological and biochemical processes. In this study, the effects of hydrogen sulfide coupled with calcium on cadmium removal and resistance in Phanerochaete chrysosporium were examined. The results revealed that H2S enhanced the uptake of calcium by P. chrysosporium to resist cadmium stress. The removal and accumulation of cadmium by the mycelium was reduced by H2S and Ca2+ pretreatment. Moreover, oxidative damage and membrane integrity were alleviated by H2S and Ca2+. Corresponding antioxidative enzyme activities and glutathione were also found to positively respond to H2S and Ca2+, which played an important role in the resistance to cadmium-induced oxidative stress. The effects of hydroxylamine (HA; a hydrogen sulfide inhibitor) and ethylene glycol-bis-(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA; a calcium chelator) toward H2S and Ca2+ and their cross-interactions confirmed the positive roles and the potential crosstalk of H2S and Ca2+ in cadmium stress resistance. These findings imply that the protective effects of H2S in P. chrysosporium under cadmium stress may occur through a reduction in the accumulation of cadmium and promotion of the antioxidant system, and the H2S-regulated pathway may be associated with the intracellular calcium signaling system.Key points• Altered monoterpenoid tolerance mainly related to altered activity of efflux pumps.• Increased tolerance to geranic acid surprisingly caused by decreased export activity.• Reduction of export activity can be beneficial for biotechnological conversions.

Direct production of 2, 5-Furandicarboxylicacid from raw biomass by manganese dioxide catalysis cooperated with ultrasonic-assisted diluted acid pretreatment.

In recent years, 2, 5-furandicarboxylic acid (FDCA) has attracted much attention as the precursor of bio-polyester materials. A coupled process of ultrasonic-assisted dilute acid pretreatment and MnO2 was designed in this study to directly produce FDCA from lignocellulosic biomass, which is different from the traditional preparation process. Moreover, the critical parameters in the process were analyzed and optimized by the response surface method. The yield of FDCA could reach 52.1% under the optimal conditions. The reaction mechanism indicated that heavy metal elements in lignocellulosic biomass could play the role of the Lewis acid catalyst to promote the formation of FDCA to a certain extent. With the increase of temperature, the heavy metals transfer in biomass from the solid phase to the liquid phase increased, but most of them remain in the former. Therefore, further purification and treatment measures are worthy of attention.

Single and simultaneous adsorption of pefloxacin and Cu(II) ions from aqueous solutions by oxidized multiwalled carbon nanotube.

In this study, the oxidized multiwalled carbon nanotube (O-MWCNTs) was obtained by a simple method, and investigated by various techniques (SEM, TEM, FT-IR, XPS and zeta potential) for the removal of pefloxacin and Cu(II). The mutual effects of their adsorption onto O-MWCNTs were comprehensively clarified with sole and binary systems with adsorption kinetics, sorption thermodynamic and sorption isotherm models. The results indicated that there are site enhancement and competition of pefloxacin and Cu(II) on O-MWCNTs. According to mechanism investigation on the adsorption of pefloxacin and Cu(II) by XPS analysis, pH impact study, electrostatic interaction and π-π interactions, the low concentration of Cu(II)/pefloxacin could act as a bridge between pefloxacin/Cu(II) and O-MWCNTs, which significantly enhances the adsorption of pefloxacin/Cu(II). This study provided effective method and valuable reference for the elimination of pefloxacin/Cu(II) from aquatic environments.

Concentrations and emissions of particulate matter and ammonia from extensive livestock farm in South China.

Atmospheric particulate matter (PM) and ammonia pollution from livestock feeding have gradually become the environmental concerns due to the spring up of livestock farms in worldwide. However, researches about the formation of atmospheric particulate matter related to ammonia are still limited. Therefore, a study to survey the total suspended particles (TSP), PM with the diameter less than 10 µm (PM10), PM4, PM2.5, PM1, and ammonia was conducted at four types of hog houses distinguished by its building design as well as manure handling methods in South China. Four hog houses were monitored during three fattening periods from 2016 to 2017. The emissions of NH3 per hog house averaged 210.42 µg s-1. The emissions of PM per hog house averaged 2.017 µg h-1 for PM1, 2.149 µg h-1 for PM2.5, 2.305 µg h-1 for PM4, 3.950 µg h-1 for PM10, and 9.317 µg h-1 for TSP. The emissions of PM per hog house average 2.017 µg h-1, 2.149 µg h-1, 2.305 µg h-1, 3.950 µg h-1, and 9.317 µg h-1, respectively for PM1, PM2.5, PM4, PM10, and PM10. In each hog house, while the quantity of manure determined the concentration of NH3, biological fermentation bed was able to control the ammonia volatilization compared with other three manure handling methods. The largest percentage of fine PM (< 10 µm) is produced by the manual waterless method for manure handling. When it came to the manual waterless method, largest amount of fine PM (< 10 µm) was founded to form. Among various contributions of secondary inorganic PM to PM1, the NH3 was a dominant factor. Based on our experiment, the absolute concentration of NH3 was inversely proportional to the concentration of PM1 when the background influence was removed.

Population characteristics and influential factors of nitrogen cycling functional genes in heavy metal contaminated soil remediated by biochar and compost.

Sixteen treatments of soil contaminated by Cu, Pb, and Zn by the addition of a different percentage of biochar and compost were incubated for 120 days. The abundance of denitrifying genes such as narG, nirK, nirS and nosZ and the ammonia-oxidizing amoA genes of ammonia-oxidizing archaea/bacteria (AOA/AOB), soil nitrite reductase activity (S-NiR) and their shaping factors were also determined. The relationships between functional genes, S-NiR, and physico-chemical parameters were analyzed using the Pearson correlation method. The study found that the changes in physico-chemical parameters, including water-soluble organic carbon (WSC), nitrate (NO3-) and ammonium (NH4+), were predominant in different treatments. The abundance of nirK and narG genes is most sensitive to the changes in the properties of the soil sample. Bacterial 16S rDNA gene abundance was significantly affected by NO3- and S-NiR (P < 0.05). Nitrifying genes were mainly correlated to WSC and S-NiR, while denitrifying genes were associated with pH, electrical conductivity, NO3- and S-NiR. The systematic study for the relationship between the genes and the environmental parameters will help us to deep understand the biological mechanisms of nitrogen cycle in heavy metal contaminated soils remediated by biochar and compost.

Key environmental factors to variation of ammonia-oxidizing archaea community and potential ammonia oxidation rate during agricultural waste composting.

In this research, the abundance and structure of AOA amoA gene during agricultural waste composting were determined by quantitative PCR and sequencing techniques, respectively. Pairwise correlations between potential ammonia oxidation (PAO) rate, physicochemical parameters and the AOA abundance were evaluated using Pearson correlation coefficient. Relationships between these parameters, PAO rates and AOA community structure were evaluated by redundancy analysis. Results showed that 22 AOA gene OTUs were divided into the soil/sediment lineage by phylogenetic analyses. Significant positive correlations were obtained between AOA amoA gene abundance and moisture, ammonium, water soluble carbon (WSC) and organic matter (OM), respectively. Redundancy analysis showed OM, pH and nitrate significantly explained the AOA amoA gene structure. Pearson correlation revealed the PAO rate correlated positively to ammonium, AOA amoA gene abundance. These results indicated that AOA communities sense the fluctuations in surrounding environment, and ultimately react and influence the nitrogen transformation during agricultural waste composting.

Comparisons of three plant species in accumulating polycyclic aromatic hydrocarbons (PAHs) from the atmosphere: a review.

Plant leaves play a key role in the accumulation of PAHs, as they are able to capture PAHs from the air. In this paper, the mechanism, including absorption and adsorption, for plants to scavenge PAHs from the air was reviewed. Moreover, the differences of PAHs accumulating capability are mainly compared among three representative plant species, including pine needles, Holm oak leaves, and moss. On the whole, it is shown that oak leaves present the strongest PAHs accumulating capability for total PAHs among three plants species. Oak leaves and pine needles show higher accumulating tendency for light and medium molecular weight PAHs, whereas moss presents stronger accumulating tendency for heavy molecular weight PAHs. Environmental factors (i.e., temperature, seasonality, and photolysis) also account for the process of PAHs transferred from air to plants. With the temperature climbing, the concentration of PAHs in the air will increase. Due to the meteorological conditions and the human activities changed with seasons, it was shown that the PAHs were greatly accumulated in leaf surface in winter than in summer. Photolysis was also able to influence the PAHs on leaf surface, which are significant to this process. In conclusion, oak, pine, and moss can be used to filter PAHs when considering urban landscaping. Besides combining the traditional analytical methods with in situ determination, there might be able to provide a novel method to further study the specific absorption mechanisms. The accumulation of PAHs in crop leaf surface related to the application of surfactants is also worth studying.

[Transfer and Fate of Polybrominated Diphenyl Ethers in an Electrical Equipment Dismantling Area Using a Multimedia Fugacity Model].

The multimedia fugacity model (Ⅲ) was used to simulate the distribution, transfer and fate of typical polybrominated diphenyl ethers (PBDEs) in air, water, soil and sediment in an electrical equipment dismantling area in eastern China. The modeling data were compared with monitored values in air, soil and sediment for validation purpose. Moreover, the transfer fluxes between different compartments were analyzed in order to infer the main transfer process. Parameters of the model were tested and the key parameters were identified using sensitivity analysis method for BDE47 and BDE209.The results of sensitivity analysis indicated that vapor pressure, the lgKow value and half-life had significant influence on concentrations of PBDEs in different media. The results showed that when the system reached equilibrium, most of the PBDEs would be accumulated in soil and sediment. The air advection outflow and soil degradation were the major routes for PBDEs to disappear in the area. The results will provide the basis for the risk management of PBDEs contamination.