Effects of WeChat-Based Mindfulness-Based Stress Reduction on Psychological Resilience and Quality of Life Among Patients Undergoing Loose Body Removal Surgery.

Background: The knee joint, a structurally intricate component, is highly susceptible to injuries, profoundly impacting the afflicted patients' life quality. Among these injuries, loose body removal stands out, historically managed through conventional open surgical interventions. However, such procedures entail complications, including infection and extended recovery periods, fostering postoperative psychological distress like anxiety and depression. Objective: This study aims to assess the impact of WeChat-based mindfulness-based stress reduction (MBSR) on psychological resilience and quality of life in patients undergoing loose body removal. Methods: A cohort of 87 patients undergoing surgical treatment for loose body removal in our hospital between November 2018 and August 2019 was enlisted. They were randomly allocated to either receive routine postoperative nursing (control group) or participate in WeChat-based MBSR (observation group) using a random number table method. Assessment parameters encompassed anxiety, depression, mindfulness levels, quality of life, personality traits, and psychological resilience. Results: After treatment, patients in the observation group exhibited significantly reduced levels of depression, anxiety, and heightened mindfulness compared to the control group (P < .05). Moreover, the observation group reported superior quality of life and personality traits compared to the control group (P < .05). Additionally, patients in the observation group demonstrated enhanced psychological resilience compared to the control group (P < .05). Conclusions: WeChat-based MBSR emerges as a promising intervention, significantly alleviating depression and anxiety while enhancing the quality of life and psychological resilience in patients undergoing loose body removal surgery, suggesting its potential for widespread clinical application.

[Constructing the recombinant of pET28a-TAT-LacZ].

A synthesized double-stranded oligomeric nucleotide encoding 11-amino acid TAT protein transduction domain3 was inserted into pET28a vector after 6 histidine coding sequence, LacZ gene from pcDNA4/Myc-His/LacZ was digested by EcoR I and Hind III, then cloned into pET28a-TAT. The highly expressed TAT-beta-galactosidase was purified by affinity chromatography. TAT-beta-Gal fusion protein can across vascular smooth muscle cells in vitro. This result open new possibility for direct delivery of protein into tissues for therapy.