RESUMO
Digital holographic microscopy is an imaging process that encodes the 3D information of a sample into a single 2D hologram. The holographic reconstruction that decodes the hologram is conventionally based on the diffraction formula and involves various iterative steps in order to recover the lost phase information of the hologram. In the past few years, the deep-learning-based model has shown great potential to perform holographic reconstruction directly on a single hologram. However, preparing a large and high-quality dataset to train the models remains a challenge, especially when the holographic reconstruction images that serve as ground truth are difficult to obtain and can have a deteriorated quality due to various interferences of the imaging device. A cycle generative adversarial network is first trained with unpaired brightfield microscope images to restore the visual quality of the holographic reconstructions. The enhanced holographic reconstructions then serve as ground truth for the supervised learning of a U-Net that performs the holographic reconstruction on a single hologram. The proposed method was evaluated on plankton images and could also be applied to achieve super-resolution or colorization of the holographic reconstructions.
RESUMO
In seawater, the application of a cathodic current in a metallic structure induces the formation of a calcareous deposit formed by co-precipitation of CaCO3 and Mg(OH)2 on the metal surface. A previous study proved that this electrochemical technique is convincing as a remediation tool for dissolved nickel in seawater and that it is trapped as nickel hydroxide in the deposit. Here, the precipitation of a carbonate form with lead is studied. Pb2+ precipitation in calcareous deposit was investigated with a galvanized steel electrode by doping artificial seawater with PbCl2. Results show for the first time the presence of Pb incorporated in its carbonate form in the calcareous deposit. Trapped Pb content increased with initial Pb content in seawater. Simultaneous doping with Ni and Pb revealed that Ni trapping was favoured by higher current densities while Pb trapping was favoured by lower current densities. Finally, preliminary in situ experiments were performed in an industrial bay and validated the incorporation in real conditions of contaminants by precipitation with the calcareous deposit The present work demonstrates that co-precipitation of contaminants under their hydroxide or carbonate form in a calcareous deposit is a promising clean-up device for remediation of contaminated seawater.
RESUMO
A simple new device for dry separation of fine particulate matter from bulk soil samples is presented here. It consists of a stainless steel tube along which a nitrogen flow is imposed, resulting in the displacement of particles. Taking into account particle transport, fluid mechanics, and soil sample composition, a tube 6-m long, with a 0.04-m diameter, was found best adapted for PM10 separation. The device rapidly produced several milligrams of particulate matter, on which chemical extractions with EDTA were subsequently performed to study the kinetic parameters of extractable metals. New Caledonian mining soils were chosen here, as a case-study. Although the easily extracted metal pool represents only 0.5-6.4 % of the total metal content for the elements studied (Ni, Co, Mn), the total concentrations are extremely high. This pool is therefore far from negligible, and can be troublesome in the environment. This dry technique for fine particle separation from bulk parent soil eliminates the metal-leaching risks inherent in wet filtration and should therefore ensure safe assessment of environmental quality in fine-textured, metal-contaminated soils.