Investigating flocculation mechanisms and ecological safety of cationic guar gum for rapid harvesting of microalgal cells.

Addressing the drawbacks of traditional flocculants on microalgae biomass harvesting is crucial for large-scale industrial applications of microalgae production. In this study, cationic bioflocculant was successfully prepared by introducing cationic groups into the side chain of guar gum, achieving in-situ algae flocculation efficiency of 83.5 % with the dosage of 18.0 mg/L under pH = 10.0. Through a harmonious integration of predictive modelling and practical experimentation, a superior cell flocculation capacity of 23.5 g/g was achieved. In addition, the environmental safety and biocompatibility of cationic guar gum was assessed, using the typical suspension quantitative bacteriostatic method and the fluorescent double-staining technique. The results showed that the inhibition efficiency of Staphylococcus aureus in the system containing 60.0 mg/L cationic guar gum was only 12.0 % and there was no inhibition against Escherichia coli colonies. These findings provide a safe and green flocculant for efficient microalgae harvesting and spent medium treatment.

Performance evaluation of typical flocculants for efficient harvesting of Chlorella sorokiniana under different carbon application modes.

In this study, the growth characteristics of microalgae cultured with different carbon sources were analyzed, and the flocculation characteristics under the influence of carbon sources were evaluated using three typical flocculants. The results showed that the organic carbon sources could significantly increase the content of extracellular proteins in microalgae. Specifically, the extracellular protein concentrations of microalgae cultured with pure BG-11, ethanol, sodium acetate and glucose were 18.2 29.2, 97.3, and 34.7 mg/g, respectively. During the flocculation process, microalgae cultured with sodium acetate exhibited a weak response to the flocculant because of excessive extracellular proteins inhibited flocculation. In addition, the flocculation efficiency was also less than 50.0% cultured with sodium acetate in all pH test ranges when alum and chitosan were used as flocculants. It could be inferred that the flocculant initially happened to charge neutralization with the negatively charged proteins in the solution and then bridged the charges with the microalgae. These findings provide insights into the effects of different carbon sources on microalgal flocculation, promising organic integration of microalgae wastewater treatment and harvesting.

Urea addition as an enhanced strategy for degradation of petroleum contaminants during co-composting of straw and pig manure: Evidences from microbial community and enzyme activity evaluation.

Alterations in microbial community succession patterns and enzyme activities by petroleum pollutants during co-composting of straw and swine manure with the supplementary nitrogen source are unclear. In this study, urea was added into co-composting systems, and the removal performance of petroleum, microbial enzyme activity and community changes were investigated. Results showed that the polyphenol oxidase and catalase activities which were both related to the degradation of petroleum contaminants were accordingly increased from 20.65 to 30.31 U/g and from 171.87 to 231.86 U/g due to urea addition. The removal efficiency of petroleum contaminants in composting with urea increased from 45.06% to 82.29%. The addition of urea increased the diversity and abundance of petroleum-degrading microorganisms, and enhanced microbial linkages. This study provides a novel strategy for the degradation of petroleum hydrocarbon as well as a new insight into the effect of urea on both microbial processes and composting phases.

Responses of microalgae under different physiological phases to struvite as a buffering nutrient source for biomass and lipid production.

Microalgae cultivation for biodiesel production is promising, but the high demand for nutrients, such as nitrogen and phosphorus, remains a limiting factor. This study investigated effects of struvite, a low-cost nutrient source, on microalgae production under different physiological phases. Changes in element concentrations were determined to characterize the controllable nutrient release properties of struvite. Results showed that nutrient elements could be effectively supplemented by struvite. However, responses of microalgae under different growth stages to struvite varied obviously, achieving the highest biomass (0.53 g/L) and the lowest (0.32 g/L). Moreover, the microalgal lipid production was obviously increased by adding struvite during the growth phase, providing the first evidence that struvite could serve as an alternative buffering nutrient source to culture microalgae. The integration of microalgae cultivation with struvite as a buffering nutrient source provides a novel strategy for high ammonia nitrogen wastewater treatment with microalgae for biodiesel production.

Towards advanced nutrient removal by microalgae-bacteria symbiosis system for wastewater treatment.

In this study, the microalgae-bacteria symbiosis (ABS) system by co-culturing Chlorella sorokiniana with activated sludge was constructed for pollutants removal, and the according interaction mechanism was investigated. The results showed that the ABS system could almost completely remove ammonia nitrogen, and the removal efficiency of total nitrogen and total phosphorus could accordingly reach up to 65.3 % and 42.6 %. Brevundimonas greatly promoted microalgal biomass growth (maximum chlorophyll-a concentration of 9.4 mg/L), and microalgae contributed to the increase in the abundance of Dokdonella and Thermomonas in ABS system, thus facilitating nitrogen removal. The extended Derjaguin-Landau-Verwey-Overbeek theory indicated a repulsive potential barrier of 561.7 KT, while tryptophan-like proteins and tyrosine-like proteins were key extracellular polymeric substances for the formation of flocs by microalgae and activated sludge. These findings provide an in-depth understanding of interaction mechanism between microalgae and activated sludge for the removal of contaminants from wastewater.

Nutrient removal and lipid production by the co-cultivation of Chlorella vulgaris and Scenedesmus dimorphus in landfill leachate diluted with recycled harvesting water.

Applying microalgae for landfill leachate (LL) treatment is promising. However, LL usually needs to be diluted with much fresh water, aggravating water shortage. In this study, mono- and co-culturing microalgae (Chlorella vulgaris and Scenedesmus dimorphus) were used to treat LL diluted with recycled harvesting water, to investigate nutrient removal and lipid production. The results showed that microalgae in co-culture treatment had more biomass and stronger superoxide dismutase activity, which might be related to humic acids contained in recycled harvesting water, according to dissolved organic matters (DOMs) analysis. In addition, the lipid content and yield of co-cultured microalgae reached 27.60 % and 66.87 mg·L-1, respectively, higher than those of mono-culture, proving the potential of co-culture for the improvement of lipid production. This study provided a freshwater-saving dilution method for LL treatment with recycled harvesting water as well as a strategy for the increase of biomass and lipid accumulation by microalgae co-cultivation.

Toxic responses of freshwater microalgae Chlorella sorokiniana due to exposure of flame retardants.

Flame retardants, such as Tetrabromobisphenol A (TBBPA), Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) and tributyl phosphate (TBP), are frequently detected in surface water. However, the effects of FRs exposure on aquatic organisms especially freshwater microalgae are still unclear. In this study, the toxicities of TBBPA, TDCPP and TBP to microalgae Chlorella sorokiniana, in terms of growth inhibition, photosynthetic activity inhibition and oxidative damage, were investigated, and according ecological risks were assessed. The results showed that TBBPA, TDCPP and TBP had inhibitory effects on C. sorokiniana, with 96 h EC50 (concentration for 50% of maximal effect) values of 7.606, 41.794 and 49.996 mg/L, respectively. Fv/Fm decreased as the increase of exposure time under 15 mg/L TBBPA. Under 50 mg/L TDCPP and 80 mg/L TBP exposure, Fv/Fm decreased significantly after 24 h. However, Fv/Fm rose after 96 h, indicating that the damaged photosynthetic activity was reversible. The content of chlorophyll a decreased, as the increase of TBBPA concentration from 3 to 15 mg/L. However, chlorophyll a increased first and then decreased, as the increase of TDCPP and TBP concentrations from 0 to 50 mg/L and 0-80 mg/L, respectively. Results indicated that C. sorokiniana could use the phosphorus of TDCPP and TBP to ensure the production of chlorophyll a. The risen content of reactive oxygen species, malondialdehyde as well as superoxide dismutase activity indicated that exposure to FRs induced oxidative stress. Additionally, the risk quotients showed that tested FRs had ecological risks in natural waters or wastewaters. This study provides insights into the toxicological mechanisms of different FRs toward freshwater microalgae for better understanding of according environmental risks.

Microalgal cultivation for the upgraded biogas by removing CO2, coupled with the treatment of slurry from anaerobic digestion: A review.

Biogas is the gaseous by product generated from anaerobic digestion (AD), which is mainly composed of methane and CO2. Numerous independent studies have suggested that microalgae cultivation could achieve high efficiency for nutrient uptake or CO2 capture from AD, respectively. However, there is no comprehensive review on the purifying slurry from AD and simultaneously upgrading biogas via microalgal cultivation technology. This paper aims to fill this gap by presenting and discussing an information integration system based on microalgal technology. Furthermore, the review elaborates the mechanisms, configurations, and influencing factors of integrated system and analyzes the possible challenges for practical engineering applications and provides some feasibility suggestions eventually. There is hope that this review will offer a worthwhile and practical guideline to researchers, authorities and potential stakeholders, to promote this industry for sustainable development.

Biochar amendment of aerobic composting for the effective biodegradation of heavy oil and succession of bacterial community.

Soil pollution caused by petroleum pollutants from production trade activities in petroleum-related factories contributes serious threat to the environment and human health. Composting is technically-feasible and cost-effective in the biodegradation of heavy oil pollutants. This composting experiment was developed with four rice husk biochar (RHB) concentrations of 0 wt% (CK), 5 wt% (S1), 10 wt% (S2) and 15 wt% (S3) for the degradation of heavy oil. The results showed that RHB amendment could strengthen the degradation performance of heavy oil, and the degradation efficiencies for CK, S1, S2 and S3 were 59.67%, 65.00%, 73.29% and 74.82%, respectively. Microbial community succession process was investigated through high-throughput sequencing technology, and the RHB addition regulated bacterial community succession and further effectively facilitated the biodegradation of heavy oil in composting. This study substantiated that biochar materials-amended aerobic composting would be a promising strategy for the biodegradation of petroleum pollutants.

Evaluation of key microbial community succession and enzyme activities of nitrogen transformation in pig manure composting process through multi angle analysis.

This experiment aimed to investigate changes in enzyme activity, microbial succession, and nitrogen conversion caused by different initial carbon-to-nitrogen ratios of 25:1, 35:1 and 20:1 (namely CK, T1 and T2) during pig manure composting. The results showed that the lower carbon-to-nitrogen ratio (T2) after composting retained 19.64 g/kg of TN which was more than 16.74 and 17.32 g/kg in treatments of CK and T1, respectively, but excessive conversion of ammonium nitrogen to ammonia gas resulted in nitrogen loss. Additional straw in T1 could play the role as a bulking agent. After composting, TN in T1 retained the most, and TN contents were 63.51 %, 67.34 % and 56.24 % in CK, T1 and T2, respectively. Network analysis indicated that many types of microorganisms functioned as a whole community at various stages of nitrogen cycle. This study suggests that microbial community structure modification might be a good strategy to reduce ammonium nitrogen loss.

Nanoplastic pollution inhibits stream leaf decomposition through modulating microbial metabolic activity and fungal community structure.

Many studies have proved the impacts of nanoplastic pollution in freshwaters on aquatic organisms and ecosystems. To explore toxic mechanisms of nanoplastics on stream functioning, we conducted a microcosm experiment to investigate the effects of polystyrene nanoparticles (PS NPs, 1-100 µg L-1) on the process of leaf litter decomposition mediated by the microbial community. The chronic exposure to PS NPs at 1 and 100 µg L-1 caused significant decreases in leaf litter decomposition and nutrient (carbon and nitrogen) releases. During the ecological process, some extracellular enzymes (i.e., ß-glucosidase, glycine-aminopeptidase, and phenol oxidase) rather than fungal biomass were suppressed. Besides, decreases in the relative abundance of Anguillospora and Setophaeosphaeria weakened their functions in leaf litter decomposition. Thus, the microcosm experiment showed that PS NPs inhibited stream leaf decomposition by modulating the microbial metabolic activity and fungal community structure. Overall, the results of this study provide evidence for the consequences of nanoplastic pollution on freshwater microbial community and stream ecosystem functioning, which is conducive to evaluate the potential risks of nanoplastics in aquatic environments.

Potential threats of zeolitic imidazolate framework-8 nanoparticles to aquatic fungi associated with leaf decomposition.

Synthesis of zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs) and evaluation of their potential threats on ecosystem functioning has been reported in this work. A 45-day indoor experiment was conducted to explore the effects of ZIF-8 NPs at three different concentrations (10, 100, and 1000 µg L-1) on the aquatic fungal community associated with Populus nigra L. leaf litter decomposition. After chronic exposure, ZIF-8 NPs at 1000 µg L-1 significantly inhibited fungal biomass and extracellular enzyme activities as a result of inhibition on carbon and nitrogen loss of leaves. Besides, ZIF-8 NPs at 10 µg L-1 increased the percentage of Anguillospora in the fungal community and led Monographella cucumerina and Mycosphaerella tassiana to become the hub species, which eventually significantly promoted the decomposition of leaf litter. In conclusion, our study provides a reference for the possible ecotoxicity of ZIF-8 NPs on aquatic fungi, confirms the influence of ZIF-8 NPs on nutrient cycling in streams, and also emphasizes the importance of fungal community structure and hub species in the process of leaf litter decomposition.

Different phototoxicities of ZnO nanoparticle on stream functioning.

To explore the chronic phototoxicity of ZnO nanoparticles (NPs) on stream ecosystems, a microcosm experiment was conducted on Populus nigra L. leaf decomposition with ZnO NPs under different light components (visible and ultraviolet (UV) light) with a natural photoperiod. Light components significantly affected the transformation dynamic of ZnO NPs. After chronic exposure (day 15 to 30), ZnO NPs under light irradiation caused significant decrease in the microbial biomass, but significant increase in the fungal biomass. Compared to visible light, UV light led to lower microbial biomass and metabolic activity but higher antioxidant activity when ZnO NP concentrations were 10 and 20 mg L-1, eventually causing significant reductions in decomposition rates. Pleosporales sp., Montagnulaceae sp., and Volutella citronella responded sensitively to ZnO NPs. However, higher decomposition efficiency of leaf nitrogen was achieved under UV light when ZnO NPs concentrations were 10 mg L-1, suggesting that microbial nitrogen-related enzymes and ZnO nanoparticle photocatalytic properties contribute to leaf degradation. In conclusion, the results of this study provide compelling evidence that light components strongly affect ZnO NPs transformation, which impacts microbial communities with consequences for ecological processes in stream ecosystems.

Simulated sulfuric and nitric acid rain inhibits leaf breakdown in streams: A microcosm study with artificial reconstituted fresh water.

Acid rain containing SO42- and NO3- in China has been a public concern for decades. However, a decrease of SO2 has been recorded since the government enacted a series of policies to control its emission. To comprehensively evaluate the consequence of realistic and future acid deposition scenarios, this study explored the effects of mixed acid rain with different molar ratios of SO42- and NO3- (0:1, 1:0, 2:1, 1:1, and 1:2) on stream leaf breakdown through a microcosm experiment. A significant inhibition of leaf breakdown rate was observed when the ratio was 1:2 with reduced microcosm pH, fungal biomass, enzyme activities as well as the frequencies of hub general in the fungal community. In conclusion, the ratio of SO42- and NO3- in acid rain was an important factor that could have a profound impact on leaf breakdown, even on ecosystem structure and functioning of streams.

Insight into the potentiality of big biochar particle as an amendment in aerobic composting of sewage sludge.

To assess the potential of big biochar as composting amendment, the study was performed on aerobic composting of sewage sludge amended with litchi wood biochar (10% of fresh mixture weight, 20-40 mm) in a 400 L bioreactor system. Physicochemical properties and microbial activities were monitored during the first fermentation process of aerobic compost. Application of big biochar reduced peak temperature and shortened thermophilic phase, but increased the activities of aryl-sulfatase, ß-glucosidase, and dehydrogenase, which led to higher removal efficiency of total carbon, nitrogen, and sulphur. Big biochar inhibited degradation of organic matters in composting. Therefore, a comprehensive assessment including physicochemical and microbial properties can help to better understand the effect of big biochar on sewage sludge composting.

Insight into chronic exposure effects of nanosized titanium dioxide on Typha angustifolia leaf litter decomposition.

Advancement in nanotechnology has increased production of nanoparticles which initiates concerns for freshwater ecosystems. Nanosized TiO2 is one of the most used materials and its ecotoxicity has been extensively studied. Here, a freshwater microcosm experiment was performed to investigate the effects of nanosized (10, 60, and 100 nm) and bulk TiO2 at 1 g L-1 on the alteration in community structure of fungal decomposers and the consequences on litter decomposition of Typha angustifolia leaves. After 209 days of exposure, the decomposition rate was significantly higher in 100 nm TiO2 treatment compared to the control, which was caused by its promotion on fungal biomass and metabolic activity. Therefore, the study provides the multifaceted evidences for different effects of TiO2 with varied sizes on T. angustifolia leaf decomposition and highlights the importance of understanding the potential effects of varying sizes and long-term exposure in nanoparticle risk assessments.

The potential phototoxicity of nano-scale ZnO induced by visible light on freshwater ecosystems.

With the development of nanotechnology, nanomaterials have been widely applied in anti-bacterial coating, electronic device, and personal care products. NanoZnO is one of the most used materials and its ecotoxicity has been extensively studied. To explore the potential phototoxicity of nanoZnO induced by visible light, we conducted a long-term experiment on litter decomposition of Typha angustifolia leaves with assessment of fungal multifaceted natures. After 158 d exposure, the decomposition rate of leaf litter was decreased by nanoZnO but no additional effect by visible light. However, visible light enhanced the inhibitory effect of nanoZnO on fungal sporulation rate due to light-induced dissolution of nanoZnO. On the contrary, enzymes such as ß-glucosidase, cellobiohydrolase, and leucine-aminopeptidase were significantly increased by the interaction of nanoZnO and visible light, which led to high efficiency of leaf carbon decomposition. Furthermore, different treatments and exposure time separated fungal community associated with litter decomposition. Therefore, the study provided the evidence of the contribution of visible light to nanoparticle phototoxicity at the ecosystem level.

Can visible light impact litter decomposition under pollution of ZnO nanoparticles?

ZnO nanoparticles is one of the most used materials in a wide range including antibacterial coating, electronic device, and personal care products. With the development of nanotechnology, ecotoxicology of ZnO nanoparticles has been received increasing attention. To assess the phototoxicity of ZnO nanoparticles in aquatic ecosystem, microcosm experiments were conducted on Populus nigra L. leaf litter decomposition under combined effect of ZnO nanoparticles and visible light radiation. Litter decomposition rate, pH value, extracellular enzyme activity, as well as the relative contributions of fungal community to litter decomposition were studied. Results showed that long-term exposure to ZnO nanoparticles and visible light led to a significant decrease in litter decomposition rate (0.26 m-1 vs 0.45 m-1), and visible light would increase the inhibitory effect (0.24 m-1), which caused significant decrease in pH value of litter cultures, fungal sporulation rate, as well as most extracellular enzyme activities. The phototoxicity of ZnO nanoparticles also showed impacts on fungal community composition, especially on the genus of Varicosporium, whose abundance was significantly and positively related to decomposition rate. In conclusion, our study provides the evidence for negatively effects of ZnO NPs photocatalysis on ecological process of litter decomposition and highlights the contribution of visible light radiation to nanoparticles toxicity in freshwater ecosystems.