Action of chitosan-based solutions against a model four-species biofilm formed on cobalt-chromium and acrylic resin surfaces.

OBJECTIVE: To evaluate the anti-biofilm action of chitosan, nanoparticulate chitosan, and denture cleanser Nitradine™ against biofilms comprising Candida albicans, Candida glabrata, Staphylococcus aureus, and Streptococcus mutans. BACKGROUND: Biofilm removal from removable partial dentures (RPD) is important for success in prosthetic rehabilitation. MATERIALS AND METHODS: The anti-biofilm action of the experimental chitosan-based solutions and Nitradine™ was evaluated on acrylic resin and cobalt-chromium alloy through assessing cell viability, cell metabolism, residual aggregated biofilm, and extracellular polymeric substance and biofilm morphology. RESULTS: Only chitosan reduced the viability of C. albicans on cobalt-chromium alloy surface, by 98% (a 1.7 log10 reduction in cfu). Chitosan-based solutions neither promoted substantial alteration of the metabolic activity of the four-species biofilm nor reduced the amount of the aggregated biofilm. After immersion in chitosan and nanoparticulate chitosan, viable microorganisms and extracellular polymeric substances distributed over the entire specimens' surfaces were observed. Nitradine™ reduced the viability and metabolic activity of biofilm grown on both surfaces, but it did not remove all aggregated biofilm and extracellular polymeric substances. After immersion in Nitradine™, approximately 35% of the specimens' surfaces remained covered by aggregated biofilm, mainly composed of dead cells. CONCLUSION: Although chitosan and Nitradine™ promoted changes in the viability of microorganisms, neither solution completely removed the four-species biofilm from the Co-Cr and acrylic resin surfaces. Thus, isolated use of hygiene solutions is not indicated for biofilm control on RPDs; this requires complementary mechanical removal.

Antimicrobial Activity of Experimental Chitosan Solutions on Acrylic Resin and Cobalt-Chromium Surfaces.

PURPOSE: To evaluate the application of chitosan as a cleanser in the control of biofilm formation on cobalt-chromium (Co-Cr) alloy and acrylic resin surfaces. MATERIALS AND METHODS: In total, 172 Co-Cr discs and 172 acrylic resin discs (14 mm x 3 mm) were contaminated with Streptococcus mutans, Staphylococcus aureus, Candida albicans, or Candida glabrata and incubated for 48 hours. Then, specimens were randomly divided into groups and immersed in the following solutions for 15 minutes: solution without chitosan (WC/control); chitosan solution (CH: 5 mg/mL); chitosan nanoparticle solu.on (CN: 3.8 mg/mL); and effervescent tablet (ET). Biofilm recovery rates (n = 9) were evaluated by counting the colony-forming units (CFU/mL). Biofilm morphology was evaluated using scanning electron microscopy (SEM). Data were compared using the Kruskal-Wallis or ANOVA tests followed by the Tukey post hoc test. RESULTS: For acrylic resin, ET showed the lowest number of CFU for S aureus and S mutans (P < .001). CH exhibited intermediate values for S mutans, S aureus, and C albicans; CN exhibited intermediate values for S mutans and S aureus. For C glabrata, there was no sta.s.cal difference between the solu.ons (P = .264). For Co-Cr, ET showed the highest level of antimicrobial action against all microorganisms (P < .001), and CH showed an intermediate level of action against S mutans and S aureus. Against C albicans and C glabrata, there was no significant difference among CH, CN, and WC. CONCLUSIONS: Although ET had a broader spectrum of antimicrobial action, CH showed promise as a denture cleanser. Int J Prosthodont 2023;36:e61-e73.

Effect of Denture Cleansers on Cobalt-Chromium Alloy Surface: A Simulated Period of 5 Years' Use.

PURPOSE: To compare the effect of solutions of effervescent tablets (ET), cetylpyridinium chloride (CPC), and experimental solutions of Ricinus communis on the surface of cobalt-chromium (Co-Cr) alloys. MATERIALS AND METHODS: Fifty-five specimens of Co-Cr were prepared by the lost-wax casting method using circular patterns (∅12 × 3 mm). The specimens were randomly divided into 5 groups: deionized water (control); 2% R. communis; 10% R. communis; ET, and CPC. The surface roughness of specimens (n = 10) was evaluated before immersion (baseline), and at simulated times of ½, 1, 2, 3, 4, and 5 years, by laser confocal microscope (Sa, µm) and profilometer (Ra, µm). The surface topography and chemical composition (n = 1) was qualitatively analyzed with scanning electron microscopy (SEM), and energy dispersive X-ray spectrometry (EDS). Data were subjected to Kruskal-Wallis followed by Dunn tests, and Friedman followed by Wilcoxon tests (α = 0.05). RESULTS: For Sa, there was no difference for the solution factor. For the time factor a significant difference was found with 2% R. communis solution among baseline and ½, 2, 3, and 5 years (p < 0.001) and with 10% R. communis solution between 1 and 2 years (p = 0.007), with decreasing roughness over time. For Ra, cetylpyridinium chloride exhibited less roughness than 10% R. communis solution in ½ (p = 0.048) and 5 years (p = 0.013). In the SEM and EDS analysis the solutions did not present deleterious effects or changes in the chemical composition on the surfaces. CONCLUSIONS: Although a significant difference was found for the roughness, the results, below 0.2 µm, are clinically acceptable. Thus, all solutions can be used safely in removable partial denture cleaning for a period of 5 years.

Effect of Alkaline Peroxides on the Surface of Cobalt Chrome Alloy: An In Vitro Study.

PURPOSE: Removable denture hygiene care is very important for the longevity of the rehabilitation treatment; however, it is necessary to analyze the effects that denture cleansers can cause on the surfaces of prostheses. Thus, this study evaluated the effect of alkaline peroxide-effervescent tablets on the surface of cobalt-chromium alloys (Co-Cr) used in removable partial dentures. MATERIALS AND METHODS: Circular metallic specimens (12 × 3 mm) were fabricated and were immersed (n = 16) in: control, Polident 3 Minute (P3M), Steradent (S), Efferdent (E), Polident for Partials (PFP), and Corega Tabs (CT). The surface roughness (µm) (n = 10) was measured before and after periods of cleanser immersion corresponding to 0.5, 1, 2, 3, 4, and 5 years. Ion release was analyzed (n = 5) for Co, Cr, and molybdenum (Mo). Scanning electron microscopy (SEM) analysis and an Energy-dispersive X-ray spectroscopy (EDS) were conducted in one specimen. The surface roughness data were statistically analyzed (α = 0.05) with the Kruskal-Wallis test to compare the solutions, and the Friedman test compared the immersion durations. Ion release analysis was performed using 2-way ANOVA and Tukey's test. RESULTS: There was no significant surface roughness difference when comparing the solutions (p > 0.05) and the immersion durations (p = 0.137). Regarding ion release (µg/L), CT, E, and control produced a greater release of Co ions than S (p < 0.05). CT produced a greater release of Cr ions than control, S, and P3M (p < 0.05). Finally, E caused the greatest release of Mo ions (p < 0.05). SEM confirmed that the solutions did not damage the surfaces and EDS confirmed that there were no signs of oxidation. CONCLUSION: The various solutions tested did not have any deleterious effects on the Co-Cr alloy surface. Steradent, however, presented the smallest ionic release.