Effects of different branched-chain amino acids supplementation protocols on the inflammatory response of LPS-stimulated RAW 264.7 macrophages.

Although branched-chain amino acids (BCAA) are commonly used as a strategy to recover nutritional status of critically ill patients, recent findings on their role as immunonutrients have been associated with unfavorable outcomes, especially in obese patients. The present study aimed to explore the effects of different BCAA supplementation protocols in the inflammatory response of LPS-stimulated RAW 264.7 macrophages. Cell cultures were divided into five groups, with and without BCAA supplementation, (2 mmol/L of each amino acid). Then, cell cultures followed three different treatment protocols, consisting of a pretreatment (PT), an acute treatment (AT), and a chronic treatment (CT) with BCAA and LPS stimulation (1 µg/mL). Cell viability was analyzed by MTT assay, NO production was assessed by the Griess reaction and IL-6, IL-10, TNF-α and PGE2 synthesis, was evaluated by ELISA. BCAA significantly increased cell viability in AT and CT protocols, and NO and IL-10 synthesis in all treatment protocols. IL-6 synthesis was only increased in PT and CT protocols. TNF-α and PGE2 synthesis were not altered in any of the protocols and groups. BCAA supplementation was able to increase both pro and anti-inflammatory mediators synthesis by RAW 264.7 macrophages, which was influenced by the protocol applied. Moreover, these parameters were significantly increased by isoleucine supplementation, highlighting a potential research field for future studies.

Development of a Food Frequency Questionnaire for Brazilian athletes.

AIM: Until recently most dietary survey methods have been developed for non-athletic populations. In this study, we aimed to develop a Food Frequency Questionnaire (FFQ) to assess the regular dietary intake of Brazilian athletes. METHODS: We interviewed 141 athletes (23.36 ± 7.77 years old) for their dietary intake using 24-hour recalls. After grouping conceptually similar food items into 113 food items, percentage contribution analysis and stepwise regression models were used to highlight foods contributing to at least 90% of the between-person variability of key nutrients. RESULTS: The developed FFQ contained 59 foods which were important predictors of the variance in nutrient intake. Vitamin C and pyridoxine had the lowest number of selected foods. Conversely, 13 food items were required to explain the between-person variation for energy, carbohydrate and magnesium intake. CONCLUSIONS: Using a multiple regression analysis we developed a 59-food item questionnaire, which includes culturally specific food items and may represent an important dietary tool to analyse athletic populations. Reproducibility and validity of this FFQ will be verified in future research.

Effects of glutamine and alanine supplementation on muscle fatigue parameters of rats submitted to resistance training.

OBJECTIVE: Although glutamine and alanine have properties that could delay fatigue, recent evidence showed that these amino acids impaired central fatigue markers. Nevertheless, the effect of this intervention on muscle fatigue is unknown. The aim of this study was to investigate the effects of glutamine and alanine supplementation on muscle fatigue parameters in rats submitted to resistance training (RT). METHODS: Wistar rats were distributed into the following groups: sedentary (SED), exercised (CON), exercised and supplemented with alanine (ALA), glutamine and alanine in their free form (G+A) or l-alanyl-l-glutamine (DIP). Trained groups underwent a ladder-climbing exercise for 8 wk. In the last 3 wk of RT, supplementations were offered in water with a 4% concentration. RESULTS: G+A and DIP supplementation increased the muscle content of glutamine and glutamate. DIP administration increased glycogen and lactate dehydrogenase (LDH) concentrations in muscle, whereas ALA and G+A supplementation reduced plasma LDH and creatine kinase levels. All trained groups presented higher levels of muscle glutathione (GSH) than SED. There was no difference between groups in lactate, xanthine, hypoxanthine, thiobarbituric acid reactive substances, 8-isoprostane and GSH in plasma; adenosine monophosphate deaminase, citrate synthase and monocarboxylate transporters 1 and 4 in muscle; and glycogen and GSH in the liver. Moreover, physical performance did not differ between groups. CONCLUSION: Glutamine and alanine supplementation improved muscle fatigue markers without affecting exercise performance.

Probiotics for inflammatory bowel diseases: a promising adjuvant treatment.

Inflammatory bowel diseases (IBD) encompass ulcerative colitis (UC), Crohn's disease (CD) and indeterminate colitis (IC), characterising chronic inflammation in the gastrointestinal tract, associated with changes in the immune system and in the intestinal microbiota. Thus, probiotics may offer an alternative or adjuvant approach to conventional therapy. The present review aims to summarise the mechanisms of action of probiotics in IBD and their therapeutic effects. Most of the studies suggest that probiotics are effective in the treatment of UC, especially when several strains are concomitantly administered. Species of Lactobacillus and Bifidobacterium genres are the most commonly used, and some studies even indicate that it is possible to replace medical therapy with probiotic supplementation. Regarding CD, the results of clinical trials are controversial and do not support the use of probiotics in this disease. In conclusion, probiotic supplementation is a promising adjuvant treatment in UC, but not in CD.

Effects of Glutamine and Alanine Supplementation on Adiposity, Plasma Lipid Profile, and Adipokines of Rats Submitted to Resistance Training.

Glutamine and alanine are lipogenic and could prevent the effects of resistance training (RT) in reducing adiposity and modulating lipid profile. Thus, we aimed to investigate the effects of RT and glutamine and alanine supplementation, in their free or conjugated form, on relative epididymal adipose tissue (EAT) and brown adipose tissue (BAT) weight, plasma lipid profile, and adipokines in EAT. Thirty Wistar rats, aged two months, were distributed into five groups: control (CTRL), trained (TRN), trained and supplemented with alanine (ALA), glutamine and alanine in their free form (GLN+ALA), or L-alanyl-L-glutamine (DIP). Trained groups underwent a ladder-climbing exercise for eight weeks, with progressive load increase. Supplementations were offered in a solution with a concentration of 4% in the last 21 days of training. Food consumption and body weight gain were decreased in the TRN group compared with CTRL. RT also reduced relative EAT and BAT weight, while supplementations, especially with ALA, increased adipose tissue mass. RT reduced total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-c) (TRN vs. CTRL), whereas glutamine and alanine supplementation increased TC and LDL-c, impairing lipid profile modulation by physical exercise. RT did not affect the concentrations of adipokines in EAT, but DIP supplementation increased interleukin- (IL-) 6 and IL-10. In conclusion, RT reduced adiposity and modulated lipid profile, whereas glutamine and alanine supplementation increased adiposity and impaired lipid profile but increased the concentration of the anti-inflammatory cytokines IL-6 and IL-10 in EAT.

Probiotic supplementation in dental caries: is it possible to replace conventional treatment?

BACKGROUND: Probiotic supplementation alters oral microbiota composition and could reduce the risk or treat oral cavity diseases, such as dental caries, which are considered a public health problem. Aim: To summarize the therapeutic effects of probiotics in caries and to verify whether this intervention is capable of replacing conventional treatment in human beings. METHODS: he search of the studies was carried out in the PubMed database in October 2017, without limiting the publication period. The keyword combination used was "Probiotics" and "Dental caries." Forty-two original articles that evaluated the effect of probiotic supplementation on caries treatment in humans were included in the study. RESULTS: Most of the studies evaluated bacteria of the genus Lactobacillus. The main therapeutic effects are related to the reduction of the Streptococcus mutans oral count, increased Lactobacillus oral count, and reduction in the incidence of caries. Evidence on the therapeutic effects of the Bifidobacterium and Streptococcus genres is scarce and conflicting, making it difficult to recommend them for use in clinical practice. Only a few studies administered probiotics without conventional treatments, such as fluoride. Although probiotic supplementation presented interesting properties, the therapeutic effects are more pronounced when probiotic and fluoride are applied together. CONCLUSION: Probiotics, especially of the Lactobacillus genus, can be used as adjuvants, but cannot replace the conventional treatments of caries.

Effects of Glutamine and Alanine Supplementation on Central Fatigue Markers in Rats Submitted to Resistance Training.

Recent evidence suggests that increased brain serotonin synthesis impairs performance in high-intensity intermittent exercise and specific amino acids may modulate this condition, delaying fatigue. This study investigated the effects of glutamine and alanine supplementation on central fatigue markers in rats submitted to resistance training (RT). Wistar rats were distributed in: sedentary (SED), trained (CON), trained and supplemented with alanine (ALA), glutamine and alanine in their free form (G + A), or as dipeptide (DIP). Trained groups underwent a ladder-climbing exercise for eight weeks, with progressive loads. In the last 21 days, supplementations were offered in water with a 4% concentration. Albeit without statistically significance difference, RT decreased liver glycogen, and enhanced the concentrations of plasma glucose, free fatty acids (FFA), hypothalamic serotonin, and ammonia in muscle and the liver. Amino acids affected fatigue parameters depending on the supplementation form. G + A prevented the muscle ammonia increase by RT, whereas ALA and DIP augmented ammonia and glycogen concentrations in muscle. DIP also increased liver ammonia. ALA and G + A reduced plasma FFA, whereas DIP increased this parameter, free tryptophan/total tryptophan ratio, hypothalamic serotonin, and the serotonin/dopamine ratio. The supplementations did not affect physical performance. In conclusion, glutamine and alanine may improve or impair central fatigue markers depending on their supplementation form.

Effects of supplementation with L-glutamine and L-alanine in the body composition of rats submitted to resistance exercise / Efeitos da suplementação com L-glutamina e L-alanina na composição corporal de ratos submetidos ao exercício resistido / Efectos de la suplementación con L-glutamina y L-alanina en la composición corporal de ratones sometidos a ejercicio de resistencia

Abstract We investigated the effects of glutamine and alanine supplementation on body composition of rats submitted to resistance exercise. Wistar rats were submitted to eight-week of resistance exercise, which consisted of climbing a ladder with progressive loads (25-100% of body weight). In the last 21 days of training, animals were supplemented with L-glutamine and L-alanine, as a dipeptide or in their free form (DIP, GLN + ALA and ALA groups), or water (SED and CTRL groups). RE attenuated body weight gain and lipid contents of CTRL group (p < 0.05 vs. SED) and DIP supplementation promoted an increase in tibialis muscle weight, as well as in protein content (p < 0.05 vs. CTRL). Taken together, our data indicated that resistance exercise improves body composition and dipeptide potentiated the muscle hypertrophic effect.

Resumo Foram investigados os efeitos da suplementação com glutamina e alanina na composição corporal de ratos submetidos a exercício resistido. Ratos Wistar foram submetidos, durante oito semanas, ao exercício resistido, que consistia em subir uma escada com cargas progressivas (25 a 100% do peso corporal). Nos últimos 21 dias de treinamento, os animais foram suplementados com L-glutamina e L-alanina, como dipeptídeo ou em sua forma livre (DIP, GLN + ALA ALA e grupos) ou água (grupos SED e CTRL). Exercício resistido atenuou o ganho de peso corporal e conteúdo lipídico do CTRL (p < 0,05 vs. SED) e o DIP promoveu aumento no peso do músculo tibial, bem como no teor de proteína (p < 0,05 vs. CTRL). Os nossos dados indicam que o exercício resistido, melhora a composição corporal e dipeptídeo potencializa o efeito hipertrófico muscular.

Resumen Se investigaron los efectos de la glutamina y la alanina en la composición corporal de ratones sometidos a ejercicio de resistencia. Algunos ratones Wistar fueron sometidos a 8 semanas de ejercicio de resistencia, que consistía en subir una escalera con cargas progresivas (del 25 al 100% de la masa corporal). En los últimos 21 días, los animales recibieron un suplemento de L-glutamina y L-alanina, en forma de dipéptido o en su forma libre (grupos DIP, GLN + ALA y ALA) o agua (grupos SED y CTRL). El ejercicio de resistencia redujo el aumento de masa corporal y la concentración de lípidos del CTRL (p <0,05 vs. SED). La suplementación con DIP promovió un aumento de peso del músculo tibial, así como en el contenido de proteína (p < 0,05 frente a CTRL). Nuestros resultados indican que el ejercicio de resistencia mejora la composición corporal y el DIP potencia el efecto hipertrófico.

Pre-season dietary intake of professional soccer players.

BACKGROUND: Despite the well-documented importance of nutrition in optimizing performance and health, the dietary intake of soccer players has attracted little attention. AIM: We aimed to assess the pre-season dietary intake of professional soccer players and its adequacy in macro and micronutrients. METHODS: The pre-season dietary intake of 19 male athletes was assessed using a semi-structured 3-day food record. To determine dietary adequacy and excess, energy and macronutrient intake were compared with the Brazilian dietary reference values for athletes, and micronutrients were compared with the Estimated Average Requirement - EAR (minimum recommendation) and Tolerable Upper Intake Level - UL (maximum recommendation). RESULTS: Mean daily energy intake (40.74±12.81 kcal/kg) was adequate. However, there was a low carbohydrate intake (5.44±1.86 g/kg/day) and a high amount of protein and fat (1.91±0.75 and 1.27±0.50 g/kg/day, respectively). Sodium intake (3141.77±939.76 mg/day) was higher than UL (2300 mg/day), while the majority of players showed daily intake of vitamin A (74%), vitamin D (100%), folate (58%), calcium and magnesium (68%) below the EAR (625, 10 and 320 µg/day, 800 and 330 mg/day, respectively). CONCLUSION: The dietary intake of professional soccer players was adequate in energy, but inadequate in macro and micronutrients, which suggests the need to improve nutritional practices to sustain the physical demands of soccer during pre-season.

Winter to summer change in vitamin D status reduces systemic inflammation and bioenergetic activity of human peripheral blood mononuclear cells.

BACKGROUND: Vitamin D status [25(OH)D] has recently been reported to be associated with altered cellular bioenergetic profiles of peripheral blood mononuclear cells (PBMCs). No study has tracked the seasonal variation of 25(OH)D and its putative influence on whole body energy metabolism, cellular bioenergetic profiles, inflammatory markers and clinical chemistry. MATERIAL AND METHODS: Whole body energy metabolism and substrate utilisation were measured by indirect calorimetry. PBMCs obtained from the same subjects were isolated from whole blood, counted and freshly seeded. Bioenergetic analysis (mitochondrial stress test and glycolysis stress test) was performed using the Seahorse XFe96 flux analyser. 25(OH)D was assessed using the Architect immunoassay method. RESULTS: 25(OH)D increased by a median (IQR) of 14.40 (20.13)nmol/L (p<0.001) from winter to summer and was accompanied by significant improvements in indices of insulin sensitivity, McAuley's index (p=0.019) and quantitative insulin sensitivity check index (p=0.028). PBMC mitochondrial parameters basal respiration, non-mitochondrial respiration, ATP production, proton leak, and maximal respiration decreased in summer compared to winter. Similarly, PBMC glycolytic parameters glycolytic activity, glucose response, and glycolytic capacity were all reduced in summer compared to winter. There was also a trend for absolute resting metabolic rate (RMR) to decrease (p=0.066). Markers of systemic inflammation MCP-1, IL-6, IL-8, IL-10, and IL-12p70 decreased significantly in summer compared to winter. Participants who entered winter with a low 25(OH)D (<50nmol/L), had the greatest alteration in bioenergetic parameters in summer, relative to those with winter 25(OH)D concentrations of 50-75nmol/L or >75nmol/L. The absolute change in 25(OH)D was not associated with altered bioenergetics. CONCLUSION: Seasonal improvements in 25(OH)D was associated with reduced systemic inflammation, PBMC bioenergetic profiles and whole body energy metabolism. These observational changes in PBMC bioenergetics were most pronounced in those who had insufficient 25(OH)D in winter. The data warrants confirmation through cause and effect study designs.

Efeito da suplementação com L-glutamina e L-alanina, livres ou como dipeptídeo, sobre a lesão, inflamação e citoproteção em modelos de estresse in vivo e in vitro / Effects of supplementation with L-glutamine and L-alanine, in their free form or as dipeptide, on muscle damage, inflammation and cytoprotection of in vivo and in vitro stress models

Subprojeto 1: Determinação do efeito anti-inflamatório e citoprotetor da suplementação com L-glutamina e L-alanina, ou com L-alanil-L-glutamina (DIP) em ratos submetidos a treinamento resistido. Exercícios intensos reduzem a disponibilidade de glutamina, comprometendo a função imune e a recuperação de atletas. O objetivo do estudo foi avaliar os efeitos da suplementação oral crônica com L-glutamina e L-alanina, nas formas livres ou como dipeptídeo (DIP), sobre parâmetros de lesão, inflamação e citoproteção em ratos Wistar adultos submetidos a treinamento resistido (TR). Neste estudo, o TR reduziu a concentração de glutamina no plasma e no músculo EDL. No entanto, este efeito foi atenuado pelos suplementos contendo L-glutamina, os quais aumentaram os conteúdos da proteína de resposta ao estresse (HSP70) em células do sistema imune (PBMC) e no EDL, concomitantemente à redução da ativação do NF-kB e a da concentração de citocinas no EDL. O efeito protetor das suplementações também foi evidenciado pela atenuação de marcadores de lesão (CK e LDH) e inflamação (TNF-α e IL-1ß), bem como pelo aumento nas concentrações de marcadores anti-inflamatórios (IL-6, IL-10 e MCP-1) no plasma. Nossos resultados sugerem que a suplementação oral crônica com L-glutamina (administrada com L-alanina livre ou como DIP) promoveu efeitos citoprotetores mediados pela HSP70 em resposta à lesão e inflamação induzidas pelo TR. Subprojeto 2: Efeitos da L-alanil-L-glutamina sobre as vias de sinalização da insulina e da mTOR/S6K, e citoproteção em células musculoesqueléticas C2C12. O dipeptídeo L-alanil-L-glutamina é conhecido por modular o metabolismo e a viabilidade celular. Contudo, os efeitos sobre os componentes clássicos das vias de sinalização da insulina e da mTOR/S6K, bem como o efeito citoprotetor em células musculares, são pouco esclarecidos. O objetivo deste estudo foi investigar o efeito do DIP sobre as vias de sinalização da insulina e da mTOR/S6K em miotubos C2C12, em condições normais ou resistentes à insulina. A exposição crônica à insulina (24h) promoveu resistência à insulina, reduzindo os conteúdos totais do receptor beta (IR-ß) e do substrato do receptor de insulina (IRS-1), e diminuindo a fosforilação de IRS-1, AKT e P44/42 MAPK. Adicionalmente, houve redução na expressão do transportador de glicose (GLUT4) e HSP70, redução da viabilidade celular e menor fosforilação de p70S6k e S6, proteínas relacionadas à síntese proteica. Em contraste, a suplementação com DIP aumentou os conteúdos totais de IR-ß e IRS-1 e a fosforilação de IRS-1 e AKT. A glicólise anaeróbia e a capacidade glicolítica, além da fosforilação de p70S6k e S6, foram aumentadas pelo DIP em condições normais e na resistência à insulina. Nestas condições experimentais, nossos resultados sugerem que a suplementação com DIP melhorou as vias de sinalizações da insulina e da mTOR/S6K, aumentou a captação e metabolização da glicose, independente da estimulação com insulina e, finalmente, promoveu citoproteção resgatando parcialmente as células de um estado resistente à insulina, por meio do aumento de HSP70 e ativação das etapas finais da via mTOR/S6K.

Subproject 1: Determination of the anti-inflammatory and cytoprotective effects of supplementation with L-glutamine and L-alanine, or with L-alanyl-L-glutamine in rats submitted to resistance training. Intense exercise reduces glutamine availability, compromising immune function and recovery of athletes. The objective of the study was to evaluate the effects of chronic oral supplementation with L-glutamine and L-alanine, in their free form or as dipeptide (DIP), on muscle damage, inflammation and cytoprotection in adult Wistar rats submitted to resistance training (RT). In this study, RT reduced glutamine concentration in plasma and EDL muscle. However, this effect was attenuated by supplements containing L-glutamine, which increased the contents of the stress response protein (HSP70) in immune system cells (PBMC) and EDL, concomitantly with the reduction of NF-kB activation and the concentration of cytokines in EDL. The protective effect of supplementation was also evidenced by attenuation of lesion markers (CK and LDH) and inflammation (TNF-α and IL-1ß), as well as by the increase in anti-inflammatory plasma markers (IL-6, IL-10 and MCP-1). Our results suggest that chronic oral supplementation with L-glutamine (administered along with free L-alanine or as DIP) promoted HSP70-mediated cytoprotective effects in response to RT-induced injury and inflammation. Subproject 2: Effects of L-alanyl-L-glutamine on the components of insulin and mTOR/ S6K signaling pathways and cytoprotection in C2C12 musculoskeletal cells. The dipeptide L-alanyl-L-glutamine is known to modulate metabolism and cell viability. However, the effects on the classical components of insulin and mTOR/ S6K signaling pathways, as well as the cytoprotective effect on muscle cells, are poorly understood. The aim of this study was to investigate the effect of DIP on insulin and mTOR/ S6K signaling pathways in C2C12 myotubes, under normal or insulin resistant conditions. Chronic insulin exposure (24h) promoted insulin resistance, reducing the total contents of the insulin receptor (IR-ß) and the insulin receptor substrate (IRS-1), and decreasing the phosphorylation of IRS-1, AKT and P44/ 42 MAPK. In addition, there was a reduction in the expression of glucose transporter (GLUT4) and HSP70, reduction of cell viability and defective phosphorylation of p70S6k and S6, which are related to protein synthesis. On the other hand, DIP supplementation increased the total contents of IR-ß and IRS-1 and the phosphorylation of IRS-1 and AKT. Anaerobic glycolysis and glycolytic capacity, in addition to phosphorylation of p70S6k and S6, were increased by DIP under normal conditions and in insulin resistance. In our experimental conditions, our results suggest that DIP supplementation improved the signaling pathways of insulin and mTOR/ S6K, increased glucose uptake and metabolism, independent of insulin stimulation, and finally promoted cytoprotection by partially rescuing the cells of an insulin resistant state, by increasing HSP70 and activating the final stages of the mTOR/ S6K pathway.

[Adolescent health risk behaviors and Family Health Strategy educational activities in Cuiabá, Mato Grosso State, Brazil, 2011]. / Comportamentos de risco à saúde de adolescentes e atividades educativas da Estratégia Saúde da Família em Cuiabá, Mato Grosso, 2011.

OBJECTIVE: to analyze health risk behaviors in adolescents and Family Health Strategy (FHS) educational activities in Cuiabá, Mato Grosso State, Brazil. METHODS: a cross-sectional study was conducted using data from a survey with adolescents registered with FHS in 2011/2012; Poisson regression was used to estimate prevalence ratios (PR) and 95% confidence interval (CI95%). RESULTS: there was low participation in educational activities on alcohol (23%), food (10%) and physical activity (9%); insufficient physical activity was higher in girls (PR=0.76 - 95%CI 0.68;0.85), alcohol consumption was associated with the 15-19 age range (PR=1.48 - 95%CI 1.29;1.71) and low fruit and vegetable intake was associated with income of ≤2 minimum wages (PR=1.17 - 95%CI 1.02;1.33). CONCLUSION: there was no significant association between adolescent risk behaviors and their participation in FHS educational activities.

l-glutamine and l-alanine supplementation increase glutamine-glutathione axis and muscle HSP-27 in rats trained using a progressive high-intensity resistance exercise.

In this study we investigated the chronic effects of oral l-glutamine and l-alanine supplementation, either in their free or dipeptide form, on glutamine-glutathione (GLN-GSH) axis and cytoprotection mediated by HSP-27 in rats submitted to resistance exercise (RE). Forty Wistar rats were distributed into 5 groups: sedentary; trained (CTRL); and trained supplemented with l-alanyl-l-glutamine, l-glutamine and l-alanine in their free form (GLN+ALA), or free l-alanine (ALA). All trained animals were submitted to a 6-week ladder-climbing protocol. Supplementations were offered in a 4% drinking water solution for 21 days prior to euthanasia. Plasma glutamine, creatine kinase (CK), myoglobin (MYO), and erythrocyte concentration of reduced GSH and glutathione disulfide (GSSG) were measured. In tibialis anterior skeletal muscle, GLN-GSH axis, thiobarbituric acid reactive substances (TBARS), and the expression of heat shock factor 1 (HSF-1), 27-kDa heat shock protein (HSP-27), and glutamine synthetase were determined. In CRTL animals, high-intensity RE reduced muscle glutamine levels and increased GSSG/GSH rate and TBARS, as well as augmented plasma CK and MYO levels. Conversely, l-glutamine-supplemented animals showed an increase in plasma and muscle levels of glutamine, with a reduction in GSSG/GSH rate, TBARS, and CK. Free l-alanine administration increased plasma glutamine concentration and lowered muscle TBARS. HSF-1 and HSP-27 were high in all supplemented groups when compared with CTRL (p < 0.05). The results presented herein demonstrate that l-glutamine supplemented with l-alanine, in both a free or dipeptide form, improve the GLN-GSH axis and promote cytoprotective effects in rats submitted to high-intensity RE training.

Comportamentos de risco à saúde de adolescentes e atividades educativas da Estratégia Saúde da Família em Cuiabá, Mato Grosso, 2011 / Adolescent health risk behaviors and Family Health Strategy educational activities in Cuiabá, Mato Grosso State, Brazil, 2011 / Comportamientos de riesgo para la salud de adolescentes e actividades educativas de la Estrategia de Salud de la Familia en Cuiabá, Brasil, 2011

OBJETIVO: analisar comportamentos de risco à saúde de adolescentes e ações educativas da Estratégia Saúde da Família (ESF) em Cuiabá, Mato Grosso, Brasil. MÉTODOS: estudo transversal com dados de inquérito realizado com adolescentes cadastrados em unidades da ESF em 2011-2012; a regressão de Poisson foi empregada para estimar razões de prevalência (RP) e intervalos de confiança de 95% (IC95%). RESULTADOS: houve baixa participação em ações educativas sobre álcool (23%), alimentação (10%) e atividade física (9%); meninas apresentaram maior prevalência de atividade física insuficiente (RP=0,76 - IC95% 0,68;0,85); o consumo de álcool foi associado à idade de 15 a 19 anos (RP=1,48 - IC95% 1,29;1,71); e o baixo consumo de frutas e hortaliças associou-se à renda ≤2 salários mínimos (RP=1,17 - IC95% 1,02;1,33). CONCLUSÃO: não houve associação significativa entre os comportamentos de risco à saúde e a participação dos adolescentes em ações educativas promovidas pela ESF.

OBJECTIVE: to analyze health risk behaviors in adolescents and Family Health Strategy (FHS) educational activities in Cuiabá, Mato Grosso State, Brazil. METHODS: a cross-sectional study was conducted using data from a survey with adolescents registered with FHS in 2011/2012; Poisson regression was used to estimate prevalence ratios (PR) and 95% confidence interval (CI95%). RESULTS: there was low participation in educational activities on alcohol (23%), food (10%) and physical activity (9%); insufficient physical activity was higher in girls (PR=0.76 - 95%CI 0.68;0.85), alcohol consumption was associated with the 15-19 age range (PR=1.48 - 95%CI 1.29;1.71) and low fruit and vegetable intake was associated with income of ≤2 minimum wages (PR=1.17 - 95%CI 1.02;1.33). CONCLUSION: there was no significant association between adolescent risk behaviors and their participation in FHS educational activities.

OBJETIVO: analizar comportamientos de riesgo para la salud de adolescentes y las actividades educativas de la Estrategia de Salud de la Familia (ESF). MÉTODOS: estudio transversal con datos de las encuestas realizadas en adolescentes de Cuiabá-MT, Brasil (2011/2012); se utilizó regresión de Poisson para estimar razones de prevalencia (RP) e intervalos de confianza al 95% (IC95%). RESULTADOS: hubo baja participación en acciones educativas sobre alcohol (23%), alimentación (10%) y actividad física (9%); la inactividad física fue mayor en niñas (RP=0,76 - IC95% 0,68;0,85), el consumo de alcohol se asoció con la edad de 15 a 19 años (RP=1,48 - IC95% 1,29;1,71) y el bajo consumo de frutas y verduras con ingresos de ≤2 salarios mínimos (RP=1,17 - IC95% 1,02;1,33). CONCLUSIÓN: no hubo asociación significativa entre los comportamientos de riesgo para la salud y la participación de adolescentes en acciones promovidas por la ESF.

Determination of the anti-inflammatory and cytoprotective effects of l-glutamine and l-alanine, or dipeptide, supplementation in rats submitted to resistance exercise.

We evaluated the effects of chronic oral supplementation with l-glutamine and l-alanine in their free form or as the dipeptide l-alanyl-l-glutamine (DIP) on muscle damage, inflammation and cytoprotection, in rats submitted to progressive resistance exercise (RE). Wistar rats (n 8/group) were submitted to 8-week RE, which consisted of climbing a ladder with progressive loads. In the final 21 d before euthanasia, supplements were delivered in a 4 % solution in drinking water. Glutamine, creatine kinase (CK), lactate dehydrogenase (LDH), TNF-α, specific IL (IL-1ß, IL-6 and IL-10) and monocyte chemoattractant protein-1 (MCP-1) levels were evaluated in plasma. The concentrations of glutamine, TNF-α, IL-6 and IL-10, as well as NF-κB activation, were determined in extensor digitorum longus (EDL) skeletal muscle. HSP70 level was assayed in EDL and peripheral blood mononuclear cells (PBMC). RE reduced glutamine concentration in plasma and EDL (P<0·05 v. sedentary group). However, l-glutamine supplements (l-alanine plus l-glutamine (GLN+ALA) and DIP groups) restored glutamine levels in plasma (by 40 and 58 %, respectively) and muscle (by 93 and 105 %, respectively). GLN+ALA and DIP groups also exhibited increased level of HSP70 in EDL and PBMC, consistent with the reduction of NF-κB p65 activation and cytokines in EDL. Muscle protection was also indicated by attenuation in plasma levels of CK, LDH, TNF-α and IL-1ß, as well as an increase in IL-6, IL-10 and MCP-1. Our study demonstrates that chronic oral l-glutamine treatment (given with l-alanine or as dipeptide) following progressive RE induces cyprotective effects mediated by HSP70-associated responses to muscle damage and inflammation.

Perda hidrica e pratica de hidratacao em atletas de futebol / Water loss and hydration practices in soccer players / Perdida hidrica y practica de hidratacion en atletas de futbol

INTRODUÇÃO: Atletas de futebol sofrem grande estresse fisiológico durante os treinos. As características da atividade associadas às condições climáticas desfavoráveis podem provocar desidratação intensa resultando em redução de desempenho físico. OBJETIVO: Estimar o percentual de desidratação de atletas futebolistas durante sessões de treino na cidade de Cuiabá-MT, Brasil. MÉTODOS: Os dados foram coletados nas instalações do centro de treinamento da equipe. Participaram do estudo 17 atletas profissionais do sexo masculino (idade = 21,53 ± 1,19 anos; peso=71,99±7,66 kg; estatura=1,76±0,08m; IMC=23,31±1,69kg/m²). Foram analisadas as pesagens de pré e pós-treino em dois dias não consecutivos de treino (A e B) e pela perda de peso ocorrida, obteve-se a diferença de massa corporal (ΔMC); também se anotou toda a ingestão de líquidos. Os dados registrados foram aplicados às fórmulas específicas para análise. RESULTADOS: O ΔMC entre a medida inicial e a medida final do treino A foi de 1,08 ± 0,45 kg, já a do treino B foi de 0,85 ± 0,47kg, representando 1,5 ± 0,63% e 1,19 ± 0,59% de desidratação, respectivamente. O volume de líquido ingerido foi de 2.591 ± 440 ml no treino A e de 926 ± 356 ml no treino B. A prática do futebol reduziu a massa corporal dos atletas após o treino, indicando que houve desidratação. CONCLUSÃO: Apesar da oferta constante de água aos jogadores, observamos que a reposição hídrica ad libitum não foi suficiente para manter o estado eu-hidratado de todos os atletas. .

INTRODUCTION: Football players are subjected to great physiological stress during training. The characteristics of the activity, associated with unfavorable climatic conditions, can lead to intense dehydration resulting in a reduction in physical performance. OBJECTIVE: To estimate the percentage of dehydration in professional soccer players during training sessions in Cuiabá-MT, Brasil. METHODS: Data were collected in the team's training facility. The study included 17 male professional athletes (age = 21.53 ± 1.19 years; weight = 71.99 ± 7.66kg; stature = 1.76 ± 0.08m, BMI = 23.31 ± 1.69kg/m²). The pre- and post-training weighing in two non-consecutive days training (A and B) were obtained, and through the weight loss verified, we obtained the difference in body mass (ΔBM) and also noted all fluid intake. The recorded data were applied to the specific formulas for analysis. RESULTS: The ΔBM between the initial and final measurements was 1.08 ± 0.45kg in the training A, and training B was 0.85 ± 0.47kg, representing dehydration of 1.5 ± 0.63% and 1.19 ± 0.59%, respectively. The volume of fluid intake was 2,591 ± 440mL in the training A, and 926 ± 356mL in training B. The soccer practice reduced the body mass of athletes after training, indicating that there was dehydration. CONCLUSION: Despite the constant supply of water to the players, we found that ad libitum fluid replacement was not sufficient to maintain the hydration status of all athletes. .

INTRODUCCIÓN: Atletas del fútbol sufren mucho estrés fisiológico durante los entrenamientos. Las características de la actividad, sumadas a las condiciones climáticas desfavorables, pueden causar deshidratación intensa resultando en reducción del desempeño físico. OBJETIVO: Estimar el porcentaje de deshidratación de atletas futbolistas durante sesiones de entrenamiento en la ciudad de Cuiabá, Estado de Mato Grosso. MÉTODOS: Los datos fueron recolectados en las instalaciones del centro de entrenamiento del equipo. Participaron en el estudio 17 atletas profesionales de sexo masculino (edad = 21,53 ± 1,19 años; peso = 71,99 ± 7,66 kg; estatura = 1,76 ± 0,08m; IMC = 23,31 ± 1,69kg/m²). Se analizaron los pesos antes y después del entrenamiento, en dos días no consecutivos de entrenamiento (A y B) y según la pérdida de peso ocurrida, se obtuvo la diferencia de masa corporal (ΔMC); también se anotó toda la ingestión de líquidos. Los datos registrados fueron aplicados a las fórmulas específicas para análisis. RESULTADOS: La ΔMC entre la medida inicial y la final del entrenamiento A fue 1,08 ± 0,45 kg y la del entrenamiento B fue 0,85 ± 0,47kg, representando, respectivamente, 1,5 ± 0,63% y 1,19 ± 0,59% de deshidratación. El volumen de líquido ingerido fue 2,591 ± 440 ml en el entrenamiento A y 926 ± 356 ml en el entrenamiento B. La práctica del fútbol redujo la masa corporal de los atletas después del entrenamiento, indicando que hubo deshidratación. CONCLUSIÓN: Aun considerando el ofrecimiento constante de agua a los jugadores, observamos que la reposición hídrica ad libitum no fue suficiente para mantener el estado de hidratación propia de todos los atletas. .