Innovative enhancement of electron tunneling synergy in carbon-doped Ta2O5CuO photocatalyst with nematic liquid crystal for safe drinking water.

This study focuses on enhancing the photocatalytic properties of carbon-doped Ta2O5CuO (C-Ta2O5CuO) nanocomposites for drinking water purification. The nanocomposites were fabricated by depositing C-Ta2O5CuO onto Nematic Liquid Crystal Polaroid (NLCP) obtained from a discarded laptop monitor, employing the sputter deposition method. The X-ray diffraction (XRD) and High-resolution transmission electron microscopy (HRTEM) determined the nanocomposite thin films' crystallinity and structural properties. The EDX and XPS analyses confirmed the elemental composition and reality of the Cu-incorporated Ta2O5 nanocomposites, respectively. The combination of electron tunneling enhancement provided by the NLCP and graphitic carbon led to exceptional photocatalytic performance. This was particularly evident in the efficient degradation of P-Rosaniline Hydrochloride (PRH) dye in an aqueous medium. C-Ta2O5CuO catalytic activities were estimated at various dye concentrations, repeatability, reusability with time, and kinetics. Coating's stability and long-term activity in photocatalysis reactions were also tested. Additionally, Cu present in the C-Ta2O5CuO and ˙OH radicals exhibited remarkable bactericidal activity. They displayed significant antibacterial efficacy against both gram-positive Escherichia coli (E. coli) and gram-negative Staphylococcus aureus (S. aureus) bacteria. These findings have significant implications for the development of advanced materials with potent photocatalytic and antibacterial properties, holding promise for improving drinking water quality and addressing environmental and health challenges.

PDZD8 promotes autophagy at ER-Lysosome contact sites to regulate synaptogenesis.

Building synaptic connections, which are often far from the soma, requires coordinating a host of cellular activities from transcription to protein turnover, placing a high demand on intracellular communication. Membrane contact sites (MCSs) formed between cellular organelles have emerged as key signaling hubs for coordinating an array of cellular activities. We have found that the endoplasmic reticulum (ER) MCS tethering protein PDZD8 is required for activity-dependent synaptogenesis. PDZD8 is sufficient to drive ectopic synaptic bouton formation through an autophagy-dependent mechanism and required for basal synapse formation when autophagy biogenesis is limited. PDZD8 functions at ER-late endosome/lysosome (LEL) MCSs to promote lysosome maturation and accelerate autophagic flux. Mutational analysis of PDZD8's SMP domain further suggests a role for lipid transfer at ER-LEL MCSs. We propose that PDZD8-dependent lipid transfer from ER to LELs promotes lysosome maturation to increase autophagic flux during periods of high demand, including activity-dependent synapse formation.

Active and productive ageing in India: evidence from the time use pattern of ageing adults.

BACKGROUND: With the increasing proportion of older adults in India, it becomes essential to get an insight into the various influencing factors of successful ageing. However, the literature on successful ageing is minuscule in the Indian context. The present study attempted to understand successful ageing in terms of active and productive ageing by exploring their determining factors. METHODS: The data were extracted from the Longitudinal Ageing Study in India (LASI) Wave-1 (2017-2018). We utilized self-reported time use information from the experimental module of the LASI. A total of 7837 ageing adults were included in the study. We employed descriptive statistics, bivariate analysis and a multinominal logistic regression model to examine the prevalence and the determinants of active and productive ageing. RESULTS: The prevalence of inactive ageing was higher among the Indian ageing population (57.47%), followed by active ageing (29.59%) and productive ageing (12.94%). Poor sleep quality and the prevalence of morbidity and disability limited the ageing population from attaining active and productive ageing. Engagement in physical activity was significantly associated with active and productive ageing (ß = 0.83, 99% CI: -0.72-0.94 and ß = 0.82, 99% CI: 0.66-0.98), respectively. Rural ageing adults were more likely to attain active ageing and less likely to attain productive ageing. CONCLUSION: Engagement in physical activities among the ageing population shall be promoted to attain active and productive ageing. Since the rural ageing population were less likely to attain productive ageing than their urban counterparts, opportunities to participate in more formal economic activities in rural areas could be promoted for the wellbeing of the second demographic dividend.

Nutritional status of older adults in India: An exploration of the role of oral health and food insecurity factors.

Background: In this study, we attempted to generate insights into the determinants of nutritional status among older adults in India by exploring the role of factors including oral health, food insecurity, socioeconomic, demographic and health-related variables. The study also examined the moderating role of tooth loss with the association between chewing ability and nutritional status. Methods: The data was obtained from Longitudinal Ageing Study India (LASI) - Wave 1 (2017-18). The sample consisted of 27,411 older adults (Male = 13, 232; Female = 14, 179) aged 60 years and above. In addition to descriptive and bivariate analysis, we employed multinominal logistic regression analysis. Result: There was a significant association between the ability to chew solid food and tooth loss in the nutritional status of older adults. It was further revealed that tooth loss acted as a moderator (aRRR = 1.50, 95 % CI: 1.08-2.08) between the chewing ability and the risk of being underweight. The weight loss due to food insecurity increased the relative risk ratio of being underweight (aRRR = 1.58, 95 % CI: 1.25-2.00). Further evidence showed that self-rated health (SRH), Instrumental Activities of Daily Living (IADL), morbidity status and socioeconomic factors had a significant role in determining the nutritional status among older adults. Conclusion: Chewing ability, tooth loss, and food insecurity determine nutritional status among older adults. It is suggested to care for oral and general physical health as these factors increase the risk of nutritional deficits. Also, policy measures should be strengthened to address the specific health and food security needs of older adults from vulnerable socioeconomic circumstances.

Expanded tRNA methyltransferase family member TRMT9B regulates synaptic growth and function.

Nervous system function rests on the formation of functional synapses between neurons. We have identified TRMT9B as a new regulator of synapse formation and function in Drosophila. TRMT9B has been studied for its role as a tumor suppressor and is one of two metazoan homologs of yeast tRNA methyltransferase 9 (Trm9), which methylates tRNA wobble uridines. Whereas Trm9 homolog ALKBH8 is ubiquitously expressed, TRMT9B is enriched in the nervous system. However, in the absence of animal models, TRMT9B's role in the nervous system has remained unstudied. Here, we generate null alleles of TRMT9B and find it acts postsynaptically to regulate synaptogenesis and promote neurotransmission. Through liquid chromatography-mass spectrometry, we find that ALKBH8 catalyzes canonical tRNA wobble uridine methylation, raising the question of whether TRMT9B is a methyltransferase. Structural modeling studies suggest TRMT9B retains methyltransferase function and, in vivo, disruption of key methyltransferase residues blocks TRMT9B's ability to rescue synaptic overgrowth, but not neurotransmitter release. These findings reveal distinct roles for TRMT9B in the nervous system and highlight the significance of tRNA methyltransferase family diversification in metazoans.

Nutritional Status Predicts Injurious Falls Among Community-Dwelling Older Adults: Does Sex Matter?

The association of poor nutritional status with falls-related injuries other than fractures is unclear. Although there are sex differences in poor nutritional status and the rate of falls-related injuries, whether the impacts of poor nutritional status on falls-related injuries differ by sex is unclear. We investigated whether baseline poor nutritional status predicted injurious falls, fall-related minor injuries, and fractures at 3-year follow-up and whether these relationships differed by sex among community-dwelling older adults (N = 3257). We found that being at risk of malnutrition at baseline significantly predicted injurious falls but not minor injuries and fractures at follow-up. Compared to older males at risk of malnutrition at baseline, females at risk of malnutrition were significantly more likely to have injurious falls and minor injuries later. Being at risk of malnutrition predicted injurious falls, especially among older females. Regular nutritional screenings among older females should be implemented to provide prompt interventions against falls.

Haemostatic efficacy and inflammatory response of a novel beta-chitin patch in a cerebral small vessel injury model - A pilot study.

OBJECTIVE: Rapid and efficacious haemostasis is paramount in neurosurgery. Assessing the efficacy and short- and long-term safety of haemostatic agents utilised within cerebral tissue is essential. This pilot study investigates the haemostatic efficacy and long-term safety of a novel beta-chitin patch against traditionally used agents, bipolar and Floseal, within cerebral tissue. METHODS: Eighteen Merino sheep underwent standardised distal cortical vessel injury via temporal craniotomy. Sheep were randomised to receive 2 mls Floseal, 2 cm novel beta-chitin patch, or bipolar cautery to manage bleeding. All sheep underwent cerebral magnetic resonance imaging (MRI) at three months, before euthanasia and brain harvesting for histological assessment. RESULTS: Beta-chitin demonstrated a trend towards a faster mean time to haemostasis (TTH) compared to Floseal (223.3 ± 199 s v. 259.8 ± 186.4 s), albeit non-significant (p = 0.234). Radiologically, cerebrocortical necrosis (p = 0.842) and oedema (p = 0.368) were noted slightly more frequently in the beta-chitin group. Histologically, severe fibrotic (p = 0.017) and granulomatous changes at the craniotomy sites were only present in the beta-chitin group (p = 0.002). Neuronal degeneration was seen in all with Floseal, but beta-chitin showed a trend towards more severe reaction when present. Bipolar use predominantly showed an inflammatory cortical reaction with substantial microvascular proliferation, and Floseal showed worse severity and depth of subpial oedema, however no statistical significance was reached. CONCLUSION: All haemostats controlled bleeding, with beta-chitin demonstrating a non-inferior TTH compared to Floseal. However, it resulted in intense granulomatous and fibrotic changes, including degenerative neuronal reactions. More extensive studies are needed to assess these trends, to make further clinical inferences.

Sputter -coated N-enriched mixed metal oxides (Ta2O5-Nb2O5-N) composite: A resilient solar driven photocatalyst for water purification.

This study demonstrated the formation of N-enriched mixed metal oxides (Ta2O5-Nb2O5-N and Ta2O5-Nb2O5) thin film composites used as photocatalysts to degrade P-Rosaniline Hydrochloride (PRH-Dye) dye under solar radiation. By controlling the N gas flow rate during the sputtering process, the N concentration in the Ta2O5-Nb2O5-N composite is significantly included, and demonstrated by XPS and HRTEM analysis. With the help of XPS and HRTEM investigations, it was determined that the addition of N to Ta2O5-Nb2O5-N significantly enhances the active sites. The Ta-O-N bond (N 1 s and Ta 4p3/2 spectra) was verified by the XPS spectra. Ta2O5-Nb2O5 was found to have a lattice interplanar distance (d-spacing) of 2.52, whereas Ta2O5-Nb2O5-N showed the 2.5 (620 planes). A sputter-coated Ta2O5-Nb2O5and Ta2O5-Nb2O5-N photocatalysts were prepared, and their photocatalytic activity was evaluated using PRH-Dye as a model pollutant under solar radiation by adding H2O2 (0.01 mol). The photocatalytic activity of the Ta2O5-Nb2O5-N composite was compared with TiO2 (P-25) and Ta2O5-Nb2O5. Ta2O5-Nb2O5-N showed very high photocatalytic activity compared to Degussa P-25 TiO2 and Ta2O5-Nb2O5 under solar radiation and confirmed the presence of N in Ta2O5-Nb2O5-N significantly increased the generation of ˙OH radicals (in pH 3, 7 and 9). With the use of LC/MS, the stable intermediates or metabolite created during the photooxidation of PRH-Dye were assessed. The results of this study will provide useful insights on how Ta2O5-Nb2O5-N influences the efficiency of water pollution remediation.

Mechanical Properties, Corrosion Behavior, and In Vitro Cell Studies of the New Ti-25Ta-25Nb-5Sn Alloy.

This study aims to characterize a new Ti-25Ta-25Nb-5Sn alloy for biomedical application. Microstructure, phase formation, mechanical and corrosion properties, along with the cell culture study of the Ti-25Ta-25Nb alloy with Sn content 5 mass% are presented in this article. The experimental alloy was processed in an arc melting furnace, cold worked, and heat treated. For characterization, optical microscopy, X-ray diffraction, microhardness, and Young's modulus measurements were employed. Corrosion behavior was also evaluated using open-circuit potential (OCP) and potentiodynamic polarization. In vitro studies with human ADSCs were performed to investigate cell viability, adhesion, proliferation, and differentiation. Comparison among the mechanical properties observed in other metal alloy systems, including CP Ti, Ti-25Ta-25Nb, and Ti-25Ta-25-Nb-3Sn showed an increase in microhardness and a decrease in the Young's modulus when compared to CP Ti. The potentiodynamic polarization tests indicated that the corrosion resistance of the Ti-25Ta-25Nb-5Sn alloy was similar to CP Ti and the experiments in vitro demonstrated great interactions between the alloy surface and cells in terms of adhesion, proliferation, and differentiation. Therefore, this alloy presents potential for biomedical applications with properties required for good performance.

Robotic-Assisted Surgery-a Highly Effective Modality for Vesicovaginal Fistula Repairs.

PURPOSE OF REVIEW: To summarize recent experience with robotic vesicovaginal fistula repair and describe the contemporary technique. RECENT FINDINGS: Robotic vesicovaginal repair has been tested at multiple centers, with similar outcomes to open surgery. It has benefits including decreased hospital stay, less blood loss, and similar long-term outcomes. As long as the surgeon is experienced in robotic surgery, this technique appears to be promising. Robotic vesicovaginal repair results in similar outcomes to open surgery with decreased morbidity.

A comprehensive review of properties of the biocompatible thin films on biodegradable Mg alloys.

Magnesium (Mg) and its alloys have attracted attention as biodegradable materials for biomedical applications owing to their mechanical properties being comparable to that of bone. Mg is a vital trace element in many enzymes and thus forms one of the essential factors for human metabolism. However, before being used in biomedical applications, the early stage or fast degradation of Mg and its alloys in the physiological environment should be controlled. The degradation of Mg alloys is a critical criterion that can be controlled by a surface modification which is an effective process for conserving their desired properties. Different coating methods have been employed to modify Mg surfaces to provide good corrosion resistance and biocompatibility. This review aims to provide information on different coatings and discuss their physical and biological properties. Finally, the current withstanding challenges have been highlighted and discussed, followed by shedding some light on future perspectives.

Hepatoprotective Constituents of Macrocybe gigantea (Agaricomycetes) from India.

Non-alcoholic fatty liver disease (NAFLD) is one of the most frequent, chronic liver diseases worldwide and currently has no specific therapy. Our previous study indicated the anti-NAFLD effect of Macrocybe gigantea (Massee) Pegler & Lodge in high-fat diet-fed animals. This study aimed to isolate and identify the active hepatoprotective constituents from M. gigantea using fatty acid induced steatotic HepG2 cells as in vitro model. The effect of the test materials on the viability of HepG2 cells was analyzed using MTT assay. The HepG2 cells were treated with a mixture of palmitate-oleate to induce steatosis; after 24 h of treatment with the test materials, the intracellular lipid content was estimated using Oil Red O staining. The levels of transaminases were also estimated in the spent media. Bioassay-guided isolation of hepatoprotective constituents from M. gigantea yielded two compounds viz., ergosterol and linoleic acid; their structures were confirmed using spectroscopic data. Among these two compounds, ergosterol significantly lowered the levels of intracellular triglyceride content of fatty acid induced HepG2 cells; it also lowered the leakage of transaminases. The reductions caused by linoleic acid were not statistically significant at the tested concentrations. Detailed investigations on efficacy and safety of these compounds and M. gigantea might yield some useful leads for the management of NAFLD.

Distress return migration amid COVID-19: Kerala's response.

Emigrants from Kerala, India, were among the international migrants affected by the displacing consequences of COVID-19 - job losses, decreasing wages, inadequate social protection systems, xenophobia and overall uncertainty - which led to large-scale return migration to India. Returning home due to exogenous shocks calls into question the voluntary nature of return, the ability of returnees to reintegrate and the sustainability of re-embedding in the home country. The role of return migrants in the development of their societies of origin is also unclear. In this commentary, we explore the circumstances of return migration since the beginning of the COVID-19 pandemic by focusing on a case study of Kerala and provide insights on the future of emigration from this corridor along with policy suggestions. The role of return migrants in the development of their societies of origin requires further research and policy interventions.

pH-sensitive nanomedicine of novel tubulin polymerization inhibitor for lung metastatic melanoma.

Microtubule binding agents such as paclitaxel and vincristine have activity in metastatic melanoma. However, even responsive tumors develop resistance, highlighting the need to investigate new drug molecules. Here, we showed that a new compound, CH-2-102, developed by our group, has high anti-tumor efficacy in human and murine melanoma cells. We confirmed that CH-2-102 robustly suppresses the microtubule polymerization process by directly interacting with the colchicine binding site. Our results unveil that CH-2-102 suppresses microtubule polymerization and subsequently induces G2 phase cell arrest as one of the possible mechanisms. Notably, CH-2-102 maintains its efficacy even in the paclitaxel resistance melanoma cells due to different binding sites and a non-Pgp substrate. We developed a pH-responsive drug-polymer Schiff bases linker for high drug loading into nanoparticles (NPs). Our CH-2-102 conjugated NPs induced tumor regression more effectively than Abraxane® (Nab-paclitaxel, N-PTX), free drug, and non-sensitive NPs in B16-F10 cell-derived lung metastasis mouse model. Furthermore, our results suggest that the formulation has a high impact on the in vivo efficacy of the drug and warrants further investigation in other cancers, particularly taxane resistant. In conclusion, the microtubule polymerization inhibitor CH-2-102 conjugated pH-responsive NPs induce tumor regression in lung metastasis melanoma mice, suggesting it may be an effective strategy for treating metastatic melanoma.

BMI mediates the association of family medical history with self-reported hypertension and diabetes among older adults: Evidence from baseline wave of the longitudinal aging study in India.

Background: This study explored the association between family history of hypertension and diabetes with their diagnosis among older Indian adults. The study further examined the role of body mass index (BMI) as a potential mediator in these associations. Methods: Data from the Longitudinal Ageing Study in India (LASI, 2017-18), wave-1 were used. The sample for the study included 31,464 older adults aged 60 years and above. Descriptive statistics and bivariate analysis has been conducted to assess the prevalence of self-reported hypertension and diabetes. Further, multivariable logistic regression models were used to test the research hypotheses of this study. The Karlson-Holm-Breen (KHB) mediation analysis was conducted to recover the direct and indirect effects of BMI in the association of family medical history and diagnosis of hypertension and diabetes. Results: A proportion of 32.70% of older adults were diagnosed with hypertension and 14.23% of older adults were diagnosed with diabetes. A proportion of 19.48% and 14.69% of older adults had a family history of hypertension and diabetes, respectively. Also, 16.57% and 5.53% of older adults were overweight and obese, respectively in the current study. Older adults who had family history of hypertension had higher odds of being diagnosed with hypertension [aOR: 2.23, CI: 2.07-2.39] than those who had no such family history. This association was mediated by BMI (percent mediated: 6.31%). Similarly, older adults who had family history of diabetes had higher odds of being diagnosed with diabetes [aOR: 2.63, CI: 2.41-2.88] than those who had no such family history. This association was mediated by BMI (percent effect mediated: 6.66%). Conclusion: The study highlights the relevance of using family medical history data along with information on BMI as potential source for the control and management of hypertension and diabetes among older population.

Understanding the multifaceted impact of COVID-19 on migrants in Kerala, India.

Motivation: COVID-19 has disrupted the lives of millions of people worldwide. Migrants in developing economies have been among the most affected. This vulnerable population faces a threat to their livelihood and way of life. Hence, there is an urgent need to understand the impact of pandemic on their lives to be able to tackle subsequent waves of the pandemic or similar exogenous shocks in future. Purpose: We delve into the economic and social disruptions caused by the COVID-19 pandemic on employment, sources of income, and lives of different categories of migrant labourers in the Indian state of Kerala. Methods and approach: Using the livelihood portfolio theory, we dissect this impact in relation to a wide range of issues. This was corroborated by the in-depth semi-structured interviews with three categories of respondents. The interview data was analysed by using the directed qualitative content analysis method. We created themes from the data and juxtaposed them with the livelihood portfolio theory in addressing the research objectives. Findings: Results highlight the impact on livelihood, lifestyles, migration prospects and gender aspects. First, the households dependent on international migrants were more severely affected than those with family members who were internal migrants. Second, a considerable lifestyle change (more reliance on a plant-based diet) and borrowing patterns (more reliance on informal money lending) was reported. Third, opinions on future migration prospects were pessimistic, and a trend in favour of reverse migration was noted. We also captured the resilience measures for each of the themes. Policy implications: We find that blanket responses to mitigate migrants' hardships could be counterproductive. Policy-makers ought to implement tailor-made policies keeping in mind the migrants' classification and socio-economic demographics. Further, we recommend specific measures to address challenges that women face, to ease their workload and mitigate the loss of income. Specific measures aimed at initiating attitudinal change such as creating mental health awareness, curbing misinformation and providing counselling services could also add immense value in tackling the pandemic.

Biocompatibility and corrosion evaluation of niobium oxide coated AZ31B alloy for biodegradable implants.

Biodegradable magnesium (Mg) based implants have considerable interest in the biomedical field as their use nullifies the necessity for implant removal surgery and avoids the long-standing adverse reaction of permanent bioimplants. The degradation resistance and biocompatibility of the Mg alloys can be improved by coating them with a suitable thin film. Here, thin films of niobium and niobium oxide were developed on the AZ31B Mg alloy by sputtering technique and their biocompatibility and corrosion resistance was examined. X-ray diffraction (XRD) and Transmission electron microscope (TEM) techniques confirmed the crystallinity of the thin films. Subsequently, scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) techniques were employed to evaluate the morphology and chemical composition of the thin film surfaces, respectively. Thin-film coated Mg alloys revealed good corrosion resistance compared to their uncoated bare counterparts in simulated body fluid (SBF). The contact angle study was performed on the coated specimens to investigate their wettability which revealed their hydrophobic character. The cell viability studies on thin-film coated specimens exhibited significant cell proliferation, and cell morphological studies showed good cell attachment and growth. The in vitro MTT assay on mouse osteoblast precursor cells (MC3T3-E1) indicated that the Nb-based coatings are cytocompatible and promote cell proliferation.

Correlation of arterial PaCO2 to end tidal CO2 in children undergoing laparoscopic abdominal surgery: An observational study.

Background and Aims: The reliability of end tidal carbon dioxide (ETCO2) as a measure of arterial carbon dioxide (PaCO2) in pediatric laparoscopy is unclear. We evaluated the correlation of arterial to end tidal P(a-ET) CO2 during pediatric laparoscopy at two hours of pneumoperitoneum as the primary objective. We also compared P(a-ET) CO2 and alveolar to arterial oxygen gradient P(A-a) O2 and haemodynamics at fixed time points during surgery. Material and Methods: A cross-sectional study was conducted in 25 children undergoing laparoscopic abdominal surgery. Arterial blood gases were drawn at T0, baseline, T10: ten minutes, T1h: 1 hour, T2h: 2 hours of pnuemoperitoneum and T 10d: 10 mins after deflation. The P(a-ET) CO2, P(A-a) O2, were measured from the blood gas and ETCO2 and FiO2 values on the monitor. The Pearson's correlation coefficient, the Wilcoxon rank test and Chi square test were used for statistical analysis. Results: At T2h moderate correlation of P(a-ET) CO2 (r = 0.605, P = 0.001) with 40% children documenting accurate P(a-ET) CO2, -1 to +1 mm Hg was seen. Moderate correlation was also seen at T0, T10, T 10d but poor correlation at T 1h. The P(A-a) O2 increased progressively with surgery and did not correlate with P(a-ET) CO2. Heart rate was stable, but systolic blood pressures at T 10 and diastolic at T10, T 1h, T 2h were higher than baseline. Conclusion: Moderate correlation was seen between PaCO2 and ETCO2 at 2 h of pnuemoperitoneum and at T0, T 10, and T 10d. P(A-a) O2 increased with surgery but did not correlate with P(a-ET) CO2.