1.
J Clin Microbiol
; 39(10): 3709-11, 2001 Oct.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11574598
RESUMO
Restriction fragment length polymorphism (RFLP) analysis of IS6110 is commonly used to DNA fingerprint Mycobacterium tuberculosis. However, low-copy (< or =5) IS6110 M. tuberculosis strains are poorly differentiated, requiring secondary typing. When spoligotyping was used as the secondary method, only 13% of Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered strains had epidemiologic linkages to another patient, compared to 48% of those with high-copy strains clustered by IS6110 alone (P < 0.01). Spoligotyping did not improve a population-based molecular epidemiologic study of recent TB transmission.
Assuntos
Impressões Digitais de DNA , Elementos de DNA Transponíveis/genética , Dosagem de Genes , Mycobacterium tuberculosis/classificação , Tuberculose Pulmonar/epidemiologia , Idoso , Técnicas de Tipagem Bacteriana , Humanos , Mycobacterium tuberculosis/genética , Oligonucleotídeos/análise , Polimorfismo de Fragmento de Restrição , Tuberculose Pulmonar/microbiologia
2.
J Clin Microbiol
; 38(11): 4300, 2000 Nov.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11185066
3.
Emerg Infect Dis
; 5(6): 838-9, 1999.
Artigo
em Inglês
| MEDLINE
| ID: mdl-10603227
4.
Clin Infect Dis
; 29(3): 700, 1999 Sep.
Artigo
em Inglês
| MEDLINE
| ID: mdl-10530482