RESUMO
A 21-year-old female California kingsnake (Lampropeltis californiae) was presented to the University of Veterinary Medicine, Vienna, Austria, with a space-occupying mass in the caudal abdomen. Following clinical, radiological and sonographical evaluation the mass was removed surgically. Histopathology and transmission electron microscopy confirmed the diagnosis of a granular cell tumour, but immunohistochemical labelling for a range of markers was negative. This lesion is rare in mammals and birds, but has not been reported previously in a reptile.
Assuntos
Tumor de Células Granulares/veterinária , Abdome/patologia , Animais , Colubridae , FemininoRESUMO
CD30 is a novel therapeutic target in human mast cell (MC) neoplasms. In this 'comparative oncology' study, we examined CD30 expression and regulation in neoplastic canine MC using a panel of immunomodulatory cytokines [interleukin-2 (IL-2), IL-4, IL-5, IL-6, IL-13 and stem cell factor (SCF)] and the canine mastocytoma cell lines NI-1 and C2. Of all cytokines tested IL-4 was found to downregulate expression of CD30 in NI-1 and C2 cells. We also found that the CD30-targeting antibody-conjugate brentuximab vedotin induces growth inhibition and apoptosis in both MC lines. Next, we asked whether IL-4-induced downregulation of CD30 interferes with brentuximab vedotin-effects. Indeed, pre-incubation of NI-1 cells with IL-4 decreased responsiveness towards brentuximab vedotin. To overcome IL-4-mediated resistance, we applied drug combinations and found that brentuximab vedotin synergizes with the Kit-targeting drugs masitinib and PKC412 in inhibiting growth of NI-1 and C2 cells. In summary, CD30 is a new marker and IL-4-regulated target in neoplastic canine MC.
Assuntos
Doenças do Cão/metabolismo , Interleucina-4/fisiologia , Antígeno Ki-1/metabolismo , Mastocitose/veterinária , Animais , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Benzamidas , Brentuximab Vedotin , Linhagem Celular Tumoral , Citocinas/metabolismo , Cães , Regulação para Baixo , Sinergismo Farmacológico , Feminino , Imunoconjugados/farmacologia , Masculino , Mastocitose/metabolismo , Piperidinas , Piridinas , Estaurosporina/análogos & derivados , Estaurosporina/farmacologia , Tiazóis/farmacologiaRESUMO
One ferret (Mustela putorius furo) from Finland and two ferrets from Austria, aged 1-4.5 years and of both genders, were presented with pyogranulomatous subcutaneous inflammation affecting the inguinal, preputial and femoral regions, respectively. Histologically, microorganisms were detected within the lesions. The organisms had a capsule that stained positively by the periodic acid-Schiff reaction. Pseudomonas spp. were cultured from the lesions in two cases. In the third case, electron microscopy revealed a prokaryotic organism surrounded by an electron lucent matrix. 16S rRNA gene sequencing showed highest sequence homology to Pseudomonas luteola in all three cases. In combination with recent reports of pleuropneumonia in ferrets due to P. luteola infection, these cases might indicate a predisposition of ferrets for infection by these bacteria.
Assuntos
Paniculite/patologia , Paniculite/veterinária , Infecções por Pseudomonas/patologia , Infecções por Pseudomonas/veterinária , Animais , Feminino , Furões , MasculinoRESUMO
Although ovarian tumour in the koi (Cyprinus carpio) does not appear to be an uncommon condition, its occurrence and therapy has rarely been reported. In the present case, the decision for surgery was based on clinical and sonographic findings of an intracoelomic mass. We used tricaine methansulfonate for the anaesthesia. Laparotomy was performed by ventral access and an ovarian tumour of 12-cm diameter was removed. The wound was sutured in two layers using Vicryl®. In addition to the application of an analgesic, an antibiotic and vitamins, the postoperative conditions the patient was kept under were adapted to support wound healing. The fish recovered uneventfully and was clinically healthy during the 16-month observation period. Based on the histological findings, the tumour was diagnosed as a thecoma. Investigations using antibodies against vimentin, cytokeratin, S 100 and glial fibrillary acidic protein (GFAP) failed to provide reliable results.
Assuntos
Carpas , Doenças dos Peixes/diagnóstico , Doenças dos Peixes/cirurgia , Neoplasias Ovarianas/veterinária , Tumor da Célula Tecal/veterinária , Animais , Feminino , Doenças dos Peixes/patologia , Neoplasias Ovarianas/diagnóstico , Neoplasias Ovarianas/patologia , Neoplasias Ovarianas/cirurgia , Tumor da Célula Tecal/diagnóstico , Tumor da Célula Tecal/patologia , Tumor da Célula Tecal/cirurgiaRESUMO
Hyperthyroidism was diagnosed in four guinea pigs by demonstration of an increased serum total thyroxine concentration. The main clinical signs were comparable with those observed in feline hyperthyroidism and included weight loss despite maintenance of appetite and a palpable mass in the ventral cervical region. Three animals were treated successfully with methimazole for between 13 and 28 months. Clinical signs and regular measurement of circulating total thyroxine concentrations appear to be convenient parameters for monitoring response to medical therapy.
Assuntos
Hipertireoidismo/veterinária , Doenças dos Roedores/diagnóstico , Animais , Antitireóideos/uso terapêutico , Feminino , Cobaias , Hipertireoidismo/diagnóstico , Hipertireoidismo/tratamento farmacológico , Masculino , Metimazol/uso terapêutico , Doenças dos Roedores/tratamento farmacológico , Tiroxina/sangue , Redução de PesoRESUMO
The Pax5 gene encodes the B-cell specific activator protein (BSAP), a member of the highly conserved paired box (PAX)-domain family of transcription factors and a key regulator in the development and differentiation of B-cells. Pax5 serves as a valuable B-cell marker in the classification of human lymphoma patients as it is restricted to lymphomas of B-cell lineage. In dogs, detection of Pax5 protein in lymphoma tissue has not been reported. Therefore, we have investigated the expression and detection of BSAP using a monoclonal anti-Pax5 antibody (anti-BSAP, clone 24) in canine lymphoma tissue samples to evaluate its diagnostic relevance as a B-cell marker. A series of 25 lymph nodes from 23 canine non-Hodgkin lymphoma patients, a reactive canine lymph node, and a normal non-reactive canine lymph node, were evaluated. All B-cell non-Hodgkin lymphomas (15) were found to express Pax5 protein. In addition, there was a strong correlation between Pax5 and CD79a expression. Three CD3 positive and five CD3 and CD79a positive lymphomas were immunophenotypically negative for anti-Pax5, indicating a T-cell lineage. In conclusion, anti-Pax5 antibody may offer an excellent B-cell marker in canine lymphomas.
Assuntos
Linfócitos B/imunologia , Doenças do Cão/imunologia , Linfoma não Hodgkin/veterinária , Fator de Transcrição PAX5/biossíntese , Animais , Linfócitos B/patologia , Doenças do Cão/diagnóstico , Doenças do Cão/patologia , Cães , Feminino , Imuno-Histoquímica/veterinária , Imunofenotipagem/veterinária , Linfonodos/imunologia , Linfonodos/patologia , Linfoma não Hodgkin/diagnóstico , Linfoma não Hodgkin/imunologia , Linfoma não Hodgkin/patologia , Masculino , Fator de Transcrição PAX5/análise , Fator de Transcrição PAX5/imunologia , Inclusão em Parafina/veterináriaRESUMO
A case of endocarditis of the aortic valve in a 11 year old thoroughbred is presented. Diagnostic approach, the value of echocardiography, and the various symptoms, complications and prognosis are discussed. The horse with ruptured aortic valves due to endocarditis with severe aortic and mitral regurgitation causing congestive heart failure, was euthanised due to bad prognosis.
Assuntos
Valva Aórtica , Endocardite/veterinária , Doenças das Valvas Cardíacas/veterinária , Doenças dos Cavalos/diagnóstico , Animais , Ecocardiografia/métodos , Ecocardiografia/veterinária , Endocardite/complicações , Endocardite/diagnóstico por imagem , Eutanásia Animal , Evolução Fatal , Insuficiência Cardíaca/etiologia , Insuficiência Cardíaca/veterinária , Doenças das Valvas Cardíacas/diagnóstico por imagem , Doenças das Valvas Cardíacas/etiologia , Doenças dos Cavalos/diagnóstico por imagem , Doenças dos Cavalos/etiologia , Cavalos , Prognóstico , Ruptura Espontânea/diagnóstico por imagem , Ruptura Espontânea/etiologia , Ruptura Espontânea/veterináriaAssuntos
Adenocarcinoma/veterinária , Doenças do Gato/genética , Regulação Neoplásica da Expressão Gênica , Genes ras/genética , Mutação , Neoplasias Pancreáticas/veterinária , Adenocarcinoma/genética , Animais , Gatos , Primers do DNA , Feminino , Masculino , Neoplasias Pancreáticas/genética , Reação em Cadeia da Polimerase/veterináriaAssuntos
Adenocarcinoma/veterinária , Doenças do Cão/genética , Genes ras/genética , Neoplasias Pancreáticas/veterinária , Adenocarcinoma/genética , Animais , Primers do DNA , DNA de Neoplasias/análise , Cães , Feminino , Masculino , Mutação , Neoplasias Pancreáticas/genética , Reação em Cadeia da Polimerase/veterináriaRESUMO
Lymphomas of dogs were investigated by molecular genetic methods. Regions of exon 1 and 2 of the N-ras gene, which harbours the mutation hot spots (codons 12, 13 and 61) were screened. A GGT [symbol: see text] GAT (glycine [symbol: see text] aspartic acid) mutation in codon 13 was present in a multicentric-type lymphoma of a 1-year-old male dog.
Assuntos
Doenças do Cão/genética , Genes ras/genética , Linfoma/veterinária , Animais , Primers do DNA , Cães , Feminino , Linfoma/genética , Masculino , Mutação Puntual , Reação em Cadeia da Polimerase/veterináriaRESUMO
Haemangiosarcomas of dogs were analysed by molecular genetic techniques. Regions of the tumour suppressor gene p53, including the well-known tumour hot spots (codons 175, 245, 248, 249, 273 and 282) were screened. A 24 bp deletion was detected in exon 5 of the gene.
Assuntos
DNA de Neoplasias/genética , Doenças do Cão/genética , Genes p53/genética , Hemangiossarcoma/veterinária , Neoplasias Esplênicas/veterinária , Animais , Primers do DNA , Cães , Neoplasias Cardíacas/genética , Neoplasias Cardíacas/veterinária , Hemangiossarcoma/genética , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/veterinária , Masculino , Mutação , Reação em Cadeia da Polimerase/veterinária , Neoplasias Cutâneas/genética , Neoplasias Cutâneas/veterinária , Neoplasias Esplênicas/genéticaAssuntos
Doenças do Gato/genética , Doenças dos Bovinos/genética , Doenças do Cão/genética , Genes ras/genética , Linfoma/veterinária , Animais , Sequência de Bases , Gatos , Bovinos , Análise Mutacional de DNA , Primers do DNA , Cães , Éxons/genética , Linfoma/genética , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/veterináriaRESUMO
Tumours localised in the large bowel of dogs were subjected to molecular genetic studies. Highly conserved regions of the tumour suppressor gene p53, including typical tumour hot spots (codons 175, 245, 248, 249, 273 and 282), were analysed. A mutation CGG-->TGG (arginine-->tryptophan) was present in codon 249 in an anaplastic carcinoma in the caecum.
Assuntos
Carcinoma/genética , Carcinoma/veterinária , Doenças do Cão/genética , Genes p53/genética , Neoplasias Intestinais/genética , Neoplasias Intestinais/veterinária , Animais , Cães , Mutação/genética , Reação em Cadeia da PolimeraseRESUMO
Regions of the promoter and exons 5-8 of the tumour suppressor gene p53 were analysed in 25 cases of sporadic bovine leucosis. The study included 17 cases of juvenile leucosis, five cases of adult leucosis and three cases of skin leucosis. Exon 2 of tumour suppressor gene p16 was also investigated in the same samples. No sequence variations were present in the analysed areas of the genes. In p53, this fact represents a clear difference in comparison with enzootic bovine leucosis. In p16, no comparative data are available.
Assuntos
Leucose Enzoótica Bovina/genética , Genes p16/genética , Genes p53/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Bovinos , Infecções por Deltaretrovirus/genética , Infecções por Deltaretrovirus/veterinária , Éxons/genética , Dados de Sequência Molecular , Mutação , Regiões Promotoras Genéticas/genéticaRESUMO
OBJECTIVE: To evaluate changes of glycoconjugate in uterine glands of endometrial tissues obtained from mares. ANIMALS: adult mares. PROCEDURE: Uterine biopsy samples were collected during the breeding season and analyzed histologically for signs of chronic endometrial degeneration. Stage of the estrous cycle was established, using clinical examination and determination of hormonal status. Uterine tissue samples were analyzed, using lectin histochemical and immunohistochemical techniques (estrogen and progesterone receptors). Connective tissues were stained to determine alterations of ground substance in periglandular fibrosis. RESULTS: Of 50 mares, 30 (60%) were classified as normal or having modest alterations, and 20 (40%) were classified as having moderate or severe endometrial degeneration. In normal equine endometrium, several lectins (Helix pomatia agglutinin, Lotus tetragonolobus agglutinin, Ricinus communis I agglutinin, Ulex europaeus agglutinin, and wheat germ agglutinin) bound to glycoconjugates of the luminal epithelium and openings of uterine glands. Lectin binding patterns of cystic dilated glands or fibrotic glands in endometrial samples were remarkably strong, whereas normal surrounding cells remained unstained. Lotus tetragonolobus lectin was not suitable for detecting endometrial alterations. Connective tissues stained with Alcian blue and results of Hale colloidal-iron binding revealed acidic ground substance in periglandular fibrosis. Estrogen and progesterone receptors were evenly distributed in healthy and affected endometrial samples. CONCLUSIONS AND CLINICAL RELEVANCE: Glycoconjugate patterns of uterine glands were altered in mares with chronic endometrial degeneration. Therefore, uterine secretions are likely to be altered. These changes are not induced by changes in content of estrogen and progesterone receptors in endometrial tissues.
Assuntos
Endométrio/patologia , Doenças dos Cavalos/metabolismo , Lectinas/metabolismo , Lectinas de Plantas , Doenças Uterinas/veterinária , Animais , Biópsia/veterinária , Doença Crônica , Endométrio/metabolismo , Estrogênios/sangue , Estro , Feminino , Doenças dos Cavalos/patologia , Cavalos , Imuno-Histoquímica/veterinária , Progesterona/sangue , Receptores de Estrogênio/metabolismo , Receptores de Progesterona/metabolismo , Doenças Uterinas/metabolismo , Doenças Uterinas/patologiaRESUMO
Periglandular fibrosis and cystic dilation of uterine glands are associated with equine endometriosis. The presence of extracellular matrix proteins (collagen type I, III and IV, laminin and fibronectin) in healthy and endometriotic specimens was demonstrated by immunohistochemistry. The distribution of collagen I, but not collagen III, was dependent on the stage of the oestrous cycle. The arrangement of collagen I and collagen III in endometriotic specimens was similar to that in normal endometrium. In periglandular fibrosis, collagen IV, laminin and fibronectin deposition outside the basement membrane was observed. In these regions, stromal cells were characterized immunohistochemically as myofibroblasts because of their expression of a-smooth muscle actin, and occasionally tropomyosin and desmin. Periglandular differentiation of contractile cells could be interpreted as a reaction to support the extrusion of secretions in cystic dilated glands. Moreover, the changes of extracellular matrix proteins are characteristic for neoplastic lesions, although further development of endometriosis to benign or malignant tumours is not known in horses. Knowledge of the factors responsible for these fibroblastic modulations may be the key to explaining the pathogenesis of endometriosis.
Assuntos
Endometriose/veterinária , Proteínas da Matriz Extracelular/metabolismo , Fibroblastos/patologia , Doenças dos Cavalos/etiologia , Cavalos , Músculos/patologia , Animais , Membrana Basal/metabolismo , Diferenciação Celular , Colágeno/análise , Colágeno/metabolismo , Endometriose/metabolismo , Endometriose/patologia , Proteínas da Matriz Extracelular/análise , Feminino , Fibronectinas/análise , Fibronectinas/metabolismo , Doenças dos Cavalos/metabolismo , Doenças dos Cavalos/patologia , Imuno-Histoquímica , Laminina/análise , Laminina/metabolismo , Células Estromais/químicaRESUMO
PURPOSE: Public traffic safety campaigns in Germany have focussed on the changing risk perception of young drivers. While there is some consensus that perceptions of risk affect driving, less is understood about the relationship and interaction of alcohol consumption and risk perception on the decision to drive. We examined the influence of light alcohol consumption on risk perception and decision to drive, and the interaction of alcohol consumption and cognitive feedback on the handicapping effect of alcohol on risk perception and decision to drive. METHOD: In a double-blind block-randomized experimental study of 104 young drivers between 19 and 24 years of age, with two experimentally manipulated independent factors of alcohol consumption (three levels: 0% BAC, 0.015% BAC, 0.03% BAC) and feedback (positive or negative), we assessed three dependent variables: perception of traffic accident risk, subjective judgement about driving-relevant cognitive performance, decision to drive a car. Analyses of variance and covariance were used to analyze differences between levels of experimental factors. RESULTS: We found that persons with 0.015 BAC performed better than persons in both other alcohol conditions on the standardized risk perception task. Perceived handicap of driving was significantly more pronounced for negative feedback compared to positive feedback with no influence of the level of alcohol consumption. No significant influence on decision to drive was found of either level of alcohol consumption, feedback or sex. IMPLICATIONS: Decision to drive in young drivers could not be influenced by feedback or light consumption. Public health approaches have to find better determining factors.
Assuntos
Acidentes de Trânsito/psicologia , Intoxicação Alcoólica/psicologia , Atitude , Assunção de Riscos , Adulto , Tomada de Decisões , Método Duplo-Cego , Retroalimentação , Feminino , Humanos , MasculinoRESUMO
Ninety canine and 101 feline tumours of various types were investigated for gene mutations in the coding regions of the metastasis-associated gene S100A4 (mts1). No gene mutations were present in the analysed genomic area. A widespread histidine/tyrosine polymorphism was detected in codon 17 of S100A4.
Assuntos
Doenças do Gato/genética , Inibidor p16 de Quinase Dependente de Ciclina/genética , Doenças do Cão/genética , Neoplasias/veterinária , Sequência de Aminoácidos , Animais , Sequência de Bases , Gatos , Inibidor p16 de Quinase Dependente de Ciclina/química , DNA de Neoplasias/química , DNA de Neoplasias/isolamento & purificação , Cães , Eletroforese em Gel de Ágar/veterinária , Dados de Sequência Molecular , Neoplasias/genética , Reação em Cadeia da Polimerase/veterinária , Polimorfismo Genético/genética , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência do Ácido NucleicoRESUMO
The coding regions of tumour suppressor and cell cycle regulatory genes p21 WAF1 and p27 Kip1 were investigated in 101 feline tumours of various types. No damaging mutations were present in the analysed areas of the genes.