Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 12 de 12
Filtrar
1.
J Food Prot ; 85(2): 336-354, 2022 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-34762732

RESUMO

ABSTRACT: Extraintestinal pathogenic Escherichia coli (ExPEC) include several serotypes that have been associated with colibacillosis in poultry and with urinary tract infections (UTIs) and newborn meningitis in humans. In this study, 57 antimicrobial-resistant E. coli from apparently healthy broiler chickens were characterized for their health and safety risks. These isolates belonged to 12 serotypes, and isolates of the same serotype were clonal based on single nucleotide variant analysis. Most of the isolates harbored plasmids; IncC and IncFIA were frequently detected. The majority of the resistant isolates harbored plasmid-mediated resistance genes, including aph(3″)-Ib, aph(6)-Id, blaCMY-2, floR, sul1, sul2, tet(A), and tet(B), in agreement with their resistant phenotypes. The class 1 integron was detected in all E. coli serotypes except O124:H25 and O7:H6. Of the 57 broiler E. coli isolates, 27 were avian pathogenic, among which 18 were also uropathogenic E. coli and the remainder were other ExPEC. The two isolates of serotype O161:H4 (ST117) were genetically related to the control avian pathogenic strains and a clinical isolate associated with UTIs. A strain of serotype O159:H45 (ST101) also was closely related to a UTI isolate. The detected virulence factors included adhesins, invasins, siderophores, type III secretion systems, and toxins in combination with other virulence determinants. A broiler isolate of serotype O7:H18 (ST38) carried the ibeA gene encoding a protein involved in invasion of brain endothelium on a 102-kbp genetic island. This isolate moderately adhered and invaded Caco-2 cells and induced mortality (42.5%) in a day-old-chick infection model. The results of this study suggest that multiple antimicrobial-resistant E. coli isolates recovered from apparent healthy broilers can be pathogenic and act as reservoirs for antimicrobial resistance genes, highlighting the necessity of their assessment in a "One-Heath" context.


Assuntos
Galinhas , Farmacorresistência Bacteriana Múltipla , Escherichia coli , Animais , Antibacterianos/farmacologia , Células CACO-2 , Galinhas/microbiologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/patogenicidade , Genótipo , Humanos , Fenótipo , Virulência/genética
2.
J Food Prot ; 79(6): 929-38, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27296596

RESUMO

The objective of this study was to investigate the antibiotic resistance of Escherichia fergusonii isolated from commercial broiler chicken farms. A total of 245 isolates from cloacal and cecal samples of 28- to 36-day-old chickens were collected from 32 farms. Isolates were identified using PCR, and their susceptibility to 16 antibiotics was determined by disk diffusion assay. All isolates were susceptible to meropenem, amikacin, and ciprofloxacin. The most common resistances were against ampicillin (75.1%), streptomycin (62.9%), and tetracycline (57.1%). Of the 184 ampicillin-resistant isolates, 127 were investigated using a DNA microarray carrying 75 probes for antibiotic resistance genetic determinants. Of these 127 isolates, the ß-lactamase blaCMY2, blaTEM, blaACT, blaSHV, and blaCTX-M-15 genes were detected in 120 (94.5%), 31 (24.4%), 8 (6.3%), 6 (4.7%), and 4 (3.2%) isolates, respectively. Other detected genes included those conferring resistance to aminoglycosides (aadA1, strA, strB), trimethoprims (dfrV, dfrA1), tetracyclines (tetA, tetB, tetC, tetE), and sulfonamides (sul1, sul2). Class 1 integron was found in 35 (27.6%) of the ampicillin-resistant isolates. However, our data showed that the tested E. fergusonii did not carry any carbapenemase blaOXA genes. Pulsed-field gel electrophoresis revealed that the selected ampicillin-resistant E. fergusonii isolates were genetically diverse. The present study indicates that the monitoring of antimicrobial-resistant bacteria should include enteric bacteria such as E. fergusonii, which could be a reservoir of antibiotic resistance genes. The detection of isolates harboring extended-spectrum ß-lactamase genes, particularly blaCTX-M-15, in this work suggests that further investigations on the occurrence of such genes in broilers are warranted.


Assuntos
Galinhas/microbiologia , Farmacorresistência Bacteriana Múltipla/genética , Animais , Antibacterianos/farmacologia , Anti-Infecciosos , Proteínas de Bactérias , Farmacorresistência Bacteriana/genética , Escherichia coli/isolamento & purificação , Testes de Sensibilidade Microbiana , beta-Lactamases/genética
3.
Int J Food Microbiol ; 177: 49-56, 2014 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-24607423

RESUMO

Extraintestinal Pathogenic Escherichia coli (ExPEC) have the potential to spread through fecal waste resulting in the contamination of both farm workers and retail poultry meat in the processing plants or environment. The objective of this study was to characterize ExPEC from retail poultry meats purchased from Alberta, Canada and to compare them with 12 human ExPEC representatives from major ExPEC lineages. Fifty-four virulence genes were screened by a set of multiplex PCRs in 700 E. coli from retail poultry meat samples. ExPEC was defined as the detection of at least two of the following virulence genes: papA/papC, sfa, kpsMT II and iutA. Genetic relationships between isolates were determined using pulsed field gel electrophoresis (PFGE). Fifty-nine (8.4%) of the 700 poultry meat isolates were identified as ExPEC and were equally distributed among the phylogenetic groups A, B1, B2 and D. Isolates of phylogenetic group A possessed up to 12 virulence genes compared to 24 and 18 genes in phylogenetic groups B2 and D, respectively. E. coli identified as ExPEC and recovered from poultry harbored as many virulence genes as those of human isolates. In addition to the iutA gene, siderophore-related iroN and fyuA were detected in combination with other virulence genes including those genes encoding for adhesion, protectin and toxin while the fimH, ompT, traT, uidA and vat were commonly detected in poultry ExPEC. The hemF, iss and cvaC genes were found in 40% of poultry ExPEC. All human ExPEC isolates harbored concnf (cytotoxic necrotizing factor 1 altering cytoskeleton and causing necrosis) and hlyD (hemolysin transport) genes which were not found in poultry ExPEC. PFGE analysis showed that a few poultry ExPEC isolates clustered with human ExPEC isolates at 55-70% similarity level. Comparing ExPEC isolated from retail poultry meats provides insight into their virulence potential and suggests that poultry associated ExPEC may be important for retail meat safety. Investigations into the ability of our poultry ExPEC to cause human infections are warranted.


Assuntos
Escherichia coli/fisiologia , Microbiologia de Alimentos , Carne/microbiologia , Alberta , Animais , Técnicas de Tipagem Bacteriana , Eletroforese em Gel de Campo Pulsado , Escherichia coli/classificação , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Genes Bacterianos/genética , Humanos , Filogenia , Aves Domésticas , Sorotipagem , Fatores de Virulência/genética
4.
J Food Prot ; 77(1): 40-9, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24405997

RESUMO

The objective of this study was to analyze the antibiotic resistance phenotype and genotype of Salmonella isolated from broiler production facilities. A total of 193 Salmonella isolates recovered from commercial farms in British Columbia, Canada, were evaluated. Susceptibility to antibiotics was determined with the Sensititre system. Virulence and antibiotic resistance genes were detected by PCR assay. Genetic diversity was determined by pulse-field gel electrophoresis (PFGE) typing. Seventeen serovars of Salmonella were identified. The most prevalent Salmonella serovars were Kentucky (29.0% of isolates), Typhimurium (23.8%), Enteritidis (13.5%), and Hadar (11.9%); serovars Heidelberg, Brandenburg, and Thompson were identified in 7.7, 4.1, and 3.6% of isolates, respectively. More than 43% of the isolates were simultaneously resistant to ampicillin, amoxicillin-clavulanic acid, ceftiofur, cefoxitim, and ceftriaxone. This ß-lactam resistance pattern was observed in 33 (58.9%) of the Salmonella Kentucky isolates; 2 of these isolates were also resistant to chloramphenicol, streptomycin, sulfisoxazole, and tetracycline. Genes associated with resistance to aminoglycosides (aadA1, aadA2, and strA), ß-lactams (blaCMY-2, blaSHV, and blaTEM), tetracycline (tetA and tetB), and sulfonamide (sul1) were detected among corresponding resistant isolates. The invasin gene (invA) and the Salmonella plasmid virulence gene (spvC) were found in 97.9 and 25.9% of the isolates, respectively, with 33 (71.7%) of the 46 Salmonella Typhimurium isolates and 17 (65.4%) of the 26 Salmonella Enteritidis isolates carrying both invA and spvC. PGFE typing revealed that the antibiotic-resistant serovars were genetically diverse. These data confirm that broiler chickens can be colonized by genetically diverse antibiotic-resistant Salmonella isolates harboring virulence determinants. The presence of such strains is highly relevant to food safety and public health.


Assuntos
Antibacterianos/farmacologia , Galinhas/microbiologia , Farmacorresistência Bacteriana Múltipla/genética , Salmonella enterica , Fatores de Virulência/genética , Animais , Colúmbia Britânica , Canadá , Eletroforese em Gel de Campo Pulsado , Microbiologia de Alimentos , Inocuidade dos Alimentos , Variação Genética , Genótipo , Plasmídeos/efeitos dos fármacos , Salmonella enterica/classificação , Salmonella enterica/efeitos dos fármacos , Salmonella enterica/genética
5.
Appl Environ Microbiol ; 80(6): 1941-8, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24441160

RESUMO

Escherichia fergusonii is an emerging pathogen that has been isolated from a wide range of infections in animals and humans. Primers targeting specific genes, including yliE (encoding a conserved hypothetical protein of the cellulose synthase and regulator of cellulose synthase island), EFER_1569 (encoding a hypothetical protein, putative transcriptional activator for multiple antibiotic resistance), and EFER_3126 (encoding a putative triphosphoribosyl-dephospho-coenzyme A [CoA]), were designed for the detection of E. fergusonii by conventional and real-time PCR methods. Primers were screened by in silico PCR against 489 bacterial genomic sequences and by both PCR methods on 55 reference and field strains. Both methods were specific and sensitive for E. fergusonii, showing amplification only for this bacterium. Conventional PCR required a minimum bacterial concentration of approximately 10(2) CFU/ml, while real-time PCR required a minimum of 0.3 pg of DNA for consistent detection. Standard curves showed an efficiency of 98.5%, with an R(2) value of 0.99 for the real-time PCR assay. Cecal and cloacal contents from 580 chickens were sampled from broiler farms located in the Fraser Valley (British Columbia, Canada). Presumptive E. fergusonii isolates were recovered by enrichment and plating on differential and selective media. Of 301 total presumptive isolates, 140 (46.5%) were identified as E. fergusonii by biochemical profiling with the API 20E system and 268 (89.0%) using PCR methods. E. fergusonii detection directly from cecal and cloacal samples without preenrichment was achieved with both PCR methods. Hence, the PCR methods developed in this work significantly improve the detection of E. fergusonii.


Assuntos
Técnicas Bacteriológicas/métodos , Galinhas/microbiologia , Escherichia/classificação , Escherichia/isolamento & purificação , Técnicas de Diagnóstico Molecular/métodos , Reação em Cadeia da Polimerase Multiplex/métodos , Animais , Colúmbia Britânica , Ceco/microbiologia , Cloaca/microbiologia , Primers do DNA/genética , Escherichia/genética , Proteínas de Escherichia coli/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos
6.
Poult Sci ; 92(10): 2644-50, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24046411

RESUMO

In an effort to explore strategies to control Clostridium perfringens, we investigated the synergistic effect of a ubiquitous bacterial second messenger 3',5'-cyclic diguanylic acid (c-di-GMP) with penicillin G in a broiler challenge model. All chicks were inoculated in the crop by gavage on d 14, 15, and 16 with a mixture of 4 C. perfringens strains. Birds were treated with saline (control group) or 20 nmol of c-di-GMP by gavage or intramuscularly (IM) on d 24, all in conjunction with penicillin G in water for 5 d. Weekly samplings of ceca and ileum were performed on d 21 to 35 for C. perfringens and Lactobacillus enumeration. On d 35 of age, the IM treatment significantly (P < 0.05) reduced C. perfringens in the ceca, suggesting possible synergistic activity between penicillin G and c-di-GMP against C. perfringens in broiler ceca. Moreover, analysis of ceca DNA for the presence of a series of C. perfringens virulence genes showed a prevalence of 30% for the Clostridium perfringens alpha-toxin gene (cpa) from d 21 to 35 in the IM-treated group, whereas the occurrence of the cpa gene increased from 10 to 60% in the other 2 groups (control and gavage) from d 21 to 35. Detection of ß-lactamase genes (blaCMY-2, blaSHV, and blaTEM) indicative of gram-negative bacteria in the same samples from d 21 to 35 did not show significant treatment effects. Amplified fragment-length polymorphism showed a predominant 92% similarity between the ceca of 21-d-old control birds and the 35-d-old IM-treated c-di-GMP group. This suggests that c-di-GMP IM treatment might be effective at restoring the normal microflora of the host on d 35 after being challenged by C. perfringens. Our results suggest that c-di-GMP can reduce the colonization of C. perfringens in the gut without increasing the selection pressure for some ß-lactamase genes or altering the commensal bacterial population.


Assuntos
Adjuvantes Imunológicos/farmacologia , Galinhas , Infecções por Clostridium/veterinária , Clostridium perfringens/efeitos dos fármacos , GMP Cíclico/análogos & derivados , Enterite/veterinária , Doenças das Aves Domésticas/imunologia , Adjuvantes Imunológicos/administração & dosagem , Ração Animal/análise , Animais , Antibacterianos/administração & dosagem , Antibacterianos/farmacologia , Toxinas Bacterianas/genética , Toxinas Bacterianas/metabolismo , Ceco/microbiologia , Infecções por Clostridium/tratamento farmacológico , Infecções por Clostridium/imunologia , Clostridium perfringens/patogenicidade , Contagem de Colônia Microbiana/veterinária , GMP Cíclico/administração & dosagem , GMP Cíclico/farmacologia , Nutrição Enteral/veterinária , Enterite/tratamento farmacológico , Enterite/imunologia , Injeções Intramusculares/veterinária , Masculino , Penicilina G/administração & dosagem , Penicilina G/farmacologia , Reação em Cadeia da Polimerase/veterinária , Doenças das Aves Domésticas/tratamento farmacológico
7.
BMC Complement Altern Med ; 13: 90, 2013 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-23622254

RESUMO

BACKGROUND: Cranberry fruits possess many biological activities partly due to their various phenolic compounds; however the underlying modes of action are poorly understood. We studied the effect of cranberry fruit extracts on the gene expression of Staphylococcus aureus to identify specific cellular processes involved in the antibacterial action. METHODS: Transcriptional profiles of four S. aureus strains grown in broth supplemented or not with 2 mg/ml of a commercial cranberry preparation (Nutricran®90) were compared using DNA arrays to reveal gene modulations serving as markers for biological activity. Ethanol extracted pressed cakes from fresh fruits also produced various fractions and their effects on marker genes were demonstrated by qPCR. Minimal inhibitory concentrations (MICs) of the most effective cranberry fraction (FC111) were determined against multiple S. aureus strains and drug interactions with ß-lactam antibiotics were also evaluated. Incorporation assays with [(3)H]-radiolabeled precursors were performed to evaluate the effect of FC111 on DNA, RNA, peptidoglycan (PG) and protein biosynthesis. RESULTS: Treatment of S. aureus with Nutricran®90 or FC111 revealed a transcriptional signature typical of PG-acting antibiotics (up-regulation of genes vraR/S, murZ, lytM, pbp2, sgtB, fmt). The effect of FC111 on PG was confirmed by the marked inhibition of incorporation of D-[(3)H]alanine. The combination of ß-lactams and FC111 in checkerboard assays revealed a synergistic activity against S. aureus including strain MRSA COL, which showed a 512-fold drop of amoxicillin MIC in the presence of FC111 at MIC/8. Finally, a therapeutic proof of concept was established in a mouse mastitis model of infection. S. aureus-infected mammary glands were treated with amoxicillin, FC111 or a combination of both; only the combination significantly reduced bacterial counts from infected glands (P<0.05) compared to the untreated mice. CONCLUSIONS: The cranberry fraction FC111 affects PG synthesis of S. aureus and acts in synergy with ß-lactam antibiotics. Such a fraction easily obtained from poorly exploited press-cake residues, may find interesting applications in the agri-food sector and help reduce antibiotic usage in animal food production.


Assuntos
Antibacterianos/farmacologia , Extratos Vegetais/farmacologia , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Vaccinium macrocarpon/química , beta-Lactamas/farmacologia , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Sinergismo Farmacológico , Feminino , Humanos , Masculino , Camundongos , Extratos Vegetais/análise , Extratos Vegetais/isolamento & purificação , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus aureus/genética , Staphylococcus aureus/metabolismo
8.
Poult Sci ; 91(12): 3003-12, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23155006

RESUMO

In this study we isolated and characterized Staphylococcus xylosus, a coagulase-negative staphylococcal species considered as commensal and one of the prevalent staphylococcal species found in poultry bioaerosol. Isolates were obtained using air samplers and selective phenylethyl alcohol agar for gram-positive bacteria during 35-d periods at different times of the day. A total of 200 colonies were recovered and after basic biochemical tests were performed, presumptive staphylococci were subsequently identified by API Staph strips. A total of 153 (76.5%) staphylococci were found, among which 84 were S. xylosus (46 and 38 isolated inside and outside, respectively). Biofilm formation was observed in 86.9% of S. xylosus isolates, whereas 79.8% of them showed hemolytic activity. There was a strong correlation (92.5%) between biofilm formation and hemolytic activity. All 84 S. xylosus isolates were susceptible to amikacin, ampicillin/sulbactam, chloramphenicol, ciprofloxacin, gentamycin, kanamycin, linezolid, trimethoprim/sulfamethoxazole, and vancomycin. Resistance to nalidixic acid (86.9%), novobiocin (85.7%), penicillin (70.2%), lincomycin (46.4%), oxacillin (42.9%), ampicillin (27.4%), tetracycline (21.4%), erythromycin (11.9%), bacitracin (10.7%), and streptomycin (2.4%) was observed among the isolates. Resistance to tetracycline, lincomycin, erythromycin, and ß-lactam antibiotics was occasionally linked to the tetK, linA, ermB, and blaZ genes, respectively. Random amplification of polymorphic DNA results showed similarity of 15 to 99% between isolates collected outside and inside the barn, indicating genetic diversity of these isolates. Our study indicates that characterization of poultry bioaerosol coagulase-negative staphylococcal species such as S. xylosus is necessary for assessing their safety status for both poultry and humans.


Assuntos
Microbiologia do Ar , Galinhas , Abrigo para Animais , Staphylococcus/genética , Staphylococcus/isolamento & purificação , Aerossóis , Animais , Antibacterianos/farmacologia , DNA Bacteriano/genética , Farmacorresistência Bacteriana , Polimorfismo Genético , Especificidade da Espécie , Staphylococcus/classificação , Staphylococcus/efeitos dos fármacos , Transcriptoma
9.
Can J Microbiol ; 58(9): 1084-98, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22906311

RESUMO

The objective of this study was to characterize antimicrobial resistance and virulence determinants of Escherichia coli from soil amended with litter from 36-day-old broiler chickens ( Gallus gallus domesticus ) fed with diets supplemented with a variety of antimicrobial agents. Soil samples were collected from plots before and periodically after litter application in August to measure E. coli numbers. A total of 295 E. coli were isolated from fertilized soil samples between August and March. Antibiotic susceptibility was determined by Sensititre, and polymerase chain reaction was performed to detect the presence of resistance and virulence genes. The results confirmed that E. coli survived and could be quantified by direct plate count for at least 7 months in soil following litter application in August. The effects of feed supplementation were observed on E. coli numbers in November and January. Among the 295 E. coli, the highest antibiotic resistance level was observed against tetracycline and ß-lactams associated mainly with the resistance genes tetB and bla(CMY-2), respectively. Significant treatment effects were observed for phylogenetic groups, antibiotic resistance profiles, and virulence gene frequencies. Serotyping, phylogenetic grouping, and pulsed-field gel electrophoresis confirmed that multiple-antibiotic-resistant and potentially pathogenic E. coli can survive in soil fertilized with litter for several months regardless of antimicrobials used in the feed.


Assuntos
Antibacterianos , Dieta/veterinária , Suplementos Nutricionais , Escherichia coli/genética , Escherichia coli/metabolismo , Microbiologia do Solo , Animais , Antibacterianos/farmacologia , Galinhas/fisiologia , Farmacorresistência Bacteriana/genética , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Esterco/microbiologia , Filogenia , Densidade Demográfica , Chuva , Sorotipagem , Temperatura , Fatores de Virulência/genética
10.
Appl Environ Microbiol ; 76(24): 8033-43, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20971861

RESUMO

Enterococci are now frequent causative agents of nosocomial infections. In this study, we analyzed the frequency and distribution of antibiotic resistance and virulence genotypes of Enterococcus isolates from broiler chickens. Fecal and cecal samples from nine commercial poultry farms were collected to quantify total enterococci. Sixty-nine presumptive enterococci were isolated and identified by API 20 Strep, and their susceptibilities to antibiotics were determined. Genotypes were assessed through the use of a novel DNA microarray carrying 70 taxonomic, 17 virulence, and 174 antibiotic resistance gene probes. Total enterococcal counts were different from farm to farm and between sample sources (P < 0.01). Fifty-one (74%) of the isolates were identified as E. faecium, whereas nine (13%), seven (10%), and two (3%) isolates were identified as E. hirae, E. faecalis, and E. gallinarum, respectively. Multiple-antibiotic resistance was evident in E. faecium and E. faecalis isolates. The most common multiple-antibiotic resistance phenotype was Bac Ery Tyl Lin Str Gen Tet Cip. Genes conferring resistance to aminoglycoside (aac, aacA-aphD, aadB, aphA, sat4), macrolide (ermA, ermB, ermAM, msrC), tetracycline (tetL, tetM, tetO), streptogramin (satG_vatE8), bacitracin (bcrR), and lincosamide (linB) antibiotics were detected in corresponding phenotypes. A range of 9 to 12 different virulence genes was found in E. faecalis, including ace, agg, agrB(Efs) (agrB gene of E. faecalis), cad1, the cAM373 and cCF10 genes, cob, cpd1, cylAB, efaA(Efs), and gelE. All seven E. faecalis isolates were found to carry the gelE gene and to hydrolize gelatin and bile salts. Results from this study showed the presence of enterococci of public and environmental health concerns in broiler chicken farms and demonstrated the utility of a microarray to quickly and reliably analyze resistance and virulence genotypes of Enterococcus spp.


Assuntos
Farmacorresistência Bacteriana , Enterococcus/efeitos dos fármacos , Enterococcus/patogenicidade , Genes Bacterianos , Infecções por Bactérias Gram-Positivas/veterinária , Fatores de Virulência/genética , Animais , Carga Bacteriana , Técnicas de Tipagem Bacteriana , Ceco/microbiologia , Galinhas , Enterococcus/genética , Enterococcus/isolamento & purificação , Fezes/microbiologia , Genótipo , Infecções por Bactérias Gram-Positivas/microbiologia , Análise em Microsséries , Testes de Sensibilidade Microbiana
11.
Foodborne Pathog Dis ; 7(3): 235-41, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19911933

RESUMO

The objective of this study was to characterize antimicrobial resistance in Enterococcus spp. recovered from a commercial beef processing plant. Samples were obtained from conveyers used for moving carcasses before the start of operation (CC), 2 h after operation has started (DC), and from ground beef (GB). Randomly selected isolates from each positive sample (13 from CC; 28 from DC; 26 from GB) were confirmed to genus and species levels using PCR and the API 20 Strep kit (BioMérieux Canada, Inc., St. Laurent, Canada). A total of 199 isolates comprising 39, 84, and 76 from CC, DC, and GB, respectively, were used for antimicrobial resistance testing, major resistance genes detection, and genetic analysis. Enterococcus faecalis (87%) was the most common species found followed by Enterococcus faecium (10%). The majority of enterococci were highly associated with DC samples. About 42% of E. faecium from DC samples were resistant to quinupristin-dalfopristin. Resistance to lincomycin was observed in >90% of E. faecalis from all the three sample sources. The tetracycline-resistant enterococci (52%) were significantly higher in DC samples. Intermediate resistance to erythromycin was significantly higher in enterococci from CC and DC samples. The tetracycline and quinupristin-dalfopristin resistance in enterococci was highly correlated with the presence of tet(M) and vat(E) genes. The erm(B) gene was found in about 50% of the E. faecium isolates from GB samples and was also present in >12% of the E. faecalis isolates from all the three sample sources. Enterococci from individual sample sources were genetically similar. A number of E. faecalis from CC, DC, and GB were clustered together at >85% similarity level. These findings suggest that antimicrobial-resistant Enterococcus spp. are prevalent during commercial beef processing and can transfer between various locations in the plant and that a pool of resistance genes can be found in these enterococci.


Assuntos
Farmacorresistência Bacteriana , Enterococcus/efeitos dos fármacos , Enterococcus/isolamento & purificação , Manipulação de Alimentos/instrumentação , Carne/microbiologia , Animais , Bovinos , Ciprofloxacina , Farmacorresistência Bacteriana/genética , Enterococcus/genética , Eritromicina , Lincomicina , Testes de Sensibilidade Microbiana , Técnica de Amplificação ao Acaso de DNA Polimórfico , Resistência a Tetraciclina , Virginiamicina
12.
J Food Prot ; 72(5): 1089-93, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19517739

RESUMO

The goal of this study was to assess the distribution of antimicrobial resistance (AMR) genes in Escherichia coli isolates recovered from a commercial beef processing plant. A total of 123 antimicrobial-resistant E. coli isolates were used: 34 from animal hides, 10 from washed carcasses, 27 from conveyers for moving carcasses and meat, 26 from beef trimmings, and 26 from ground meat. The AMR genes for beta-lactamase (bla(CMY), bla(SHV), and bla(TEM), tetracycline (tet(A), tet(B), and tet(C)), sulfonamides (sul1, sul2, and sul3), and aminoglycoside (strA and strB) were detected by PCR assay. The distribution of tet(B), tet(C), sul1, bla(TEM), strA, and strB genes was significantly different among sample sources. E. coli isolates positive for the tet(B) gene and for both strA and strB genes together were significantly associated with hide, washed carcass, and ground meat samples, whereas sull gene was associated with washed carcass and beef trimming samples. The bla(TEM) gene was significantly associated with ground meat samples. About 50% of tetracycline-resistant E. coli isolates were positive for tet(A) (14%), tet(B) (15%), or tet(C) (21%) genes or both tet(B) and tet(C) genes together (3%). The sul2 gene or both sul1 and sul2 genes were found in 23% of sulfisoxazole-resistant E. coli isolates, whereas the sul3 gene was not found in any of the E. coli isolates tested. The majority of streptomycin-resistant E. coli isolates (76%) were positive for the strA and strB genes together. The bla(CMY), bla(TEM), and bla(SHV) genes were found in 12, 56, and 4%, respectively, of ampicillin-resistant E. coli isolates. These data suggest that E. coli isolates harboring AMR genes are widely distributed in meat processing environments and can create a pool of transferable resistance genes for pathogens. The results of this study underscore the need for effective hygienic and sanitation procedures in meat plants to reduce the risks of contamination with antimicrobial-resistant bacteria.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana/genética , Escherichia coli/efeitos dos fármacos , Contaminação de Alimentos/análise , Indústria de Processamento de Alimentos , Carne/microbiologia , Animais , Bovinos , Contagem de Colônia Microbiana , Qualidade de Produtos para o Consumidor , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Relação Dose-Resposta a Droga , Farmacorresistência Bacteriana Múltipla/genética , Contaminação de Equipamentos , Escherichia coli/genética , Genes Bacterianos , Humanos , Higiene , Produtos da Carne/microbiologia , Testes de Sensibilidade Microbiana
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA