An atlas of B-cell clonal distribution in the human body.

B-cell responses result in clonal expansion, and can occur in a variety of tissues. To define how B-cell clones are distributed in the body, we sequenced 933,427 B-cell clonal lineages and mapped them to eight different anatomic compartments in six human organ donors. We show that large B-cell clones partition into two broad networks-one spans the blood, bone marrow, spleen and lung, while the other is restricted to tissues within the gastrointestinal (GI) tract (jejunum, ileum and colon). Notably, GI tract clones display extensive sharing of sequence variants among different portions of the tract and have higher frequencies of somatic hypermutation, suggesting extensive and serial rounds of clonal expansion and selection. Our findings provide an anatomic atlas of B-cell clonal lineages, their properties and tissue connections. This resource serves as a foundation for studies of tissue-based immunity, including vaccine responses, infections, autoimmunity and cancer.

Novel optical switch with a reconfigurable dielectric liquid droplet.

We demonstrated a novel optical switch with a reconfigurable dielectric liquid droplet. The device consists of a clear liquid droplet (glycerol) surrounded by a black liquid (dye-doped liquid crystal). In the voltage-off state, the incident light passing through the clear liquid droplet is absorbed by the black liquid, resulting in a dark state. In the voltage-on state, the dome of the clear liquid droplet is uplifted by the dielectric force to form a light pipe which in turn transmits the incident light. Upon removing the voltage, the droplet recovers to its original shape and the switch is closed. We also demonstrated a red color light switch with ~10:1 contrast ratio and ~300 ms response time. Devices based on such an operation mechanism will find attractive applications in light shutter, tunable iris, variable optical attenuators, and displays.

A new method for fabricating high density and large aperture ratio liquid microlens array.

Conventional liquid microlens arrays are facing bottlenecks in controlling droplet size and shape, and limited aperture ratio. We report a new liquid/solvent approach to overcome these obstacles for making uniform droplet array and achieving approximately 90% aperture ratio. The droplets are very stable due to the pinning effect of the polymer walls and substrate surfaces. Using the fabricated droplet array, we demonstrate a tunable-focus microlens array based on dielectrophoretic effect. The microlens array exhibits a large dynamic range and fast response time (tens of milliseconds). Besides liquid microlens arrays, this fabrication method also opens a new door for making other tunable photonic devices.