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This paper proposes a full-automatic high-efficiency Mueller matrix microscopic imaging (MMMI) system based on the tissue microarray (TMA) for cancer inspection for the first time. By performing a polar decomposition on the sample's Mueller matrix (MM) obtained by a transmissive MMMI system we established, the linear phase retardance equivalent waveplate fast-axis azimuth and the linear phase retardance are obtained for distinguishing the cancerous tissues from the normal ones based on the differences in their polarization characteristics, where three analyses methods including statistical analysis, the gray-level co-occurrence matrix analysis (GLCM) and the Tamura image processing method (TIPM) are used. Previous MMMI medical diagnostics typically utilized discrete slices for inspection under a high-magnification objective (20×-50×) with a small field of view, while we use the TMA under a low-magnification objective (5×) with a large field of view. Experimental results indicate that MMMI based on TMA can effectively analyze the pathological variations in biological tissues, inspect cancerous cervical tissues, and thus contribute to the diagnosis of postoperative cancer biopsies. Such an inspection method, using a large number of samples within a TMA, is beneficial for obtaining consistent findings and good reproducibility.
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Processamento de Imagem Assistida por Computador , Análise Serial de Tecidos , Humanos , Análise Serial de Tecidos/métodos , Processamento de Imagem Assistida por Computador/métodos , Microscopia/métodos , Feminino , Reprodutibilidade dos Testes , Algoritmos , Neoplasias/patologia , Neoplasias do Colo do Útero/patologia , Neoplasias do Colo do Útero/diagnósticoRESUMO
Simultaneous selective determination of Fe (â ¡) and Fe (â ¢) is of great significance to the study of iron ion tracking and release of iron supplement in gastric fluid. In this paper, a composite material (N-CQDs/AgNPs/ß-CD) was prepared by a one-pot method. The various characterizations confirmed the silver nanoparticles (AgNPs) grew in situ on the surface of nitrogen-doped carbon quantum dots (N-CQDs), and the ß-cyclodextrin (ß-CD) and AgNPs linked together by Ag-O bonds finally presented gourd-like nanoparticles on the surface of N-CQDs. Then, N-CQDs/AgNPs/ß-CD modified glassy carbon electrode (GCE) was applied to detect Fe(II) and Fe(III) simultaneously. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) results confirmed that N-CQDs/AgNPs/ß-CD enhanced electrode performances because of the synergistic effect between N-CQDs, AgNPs and ß-CD. The sensor was successfully applied for the determination by differential pulse voltammetry (DPV) of Fe(II) and Fe(III) released from four iron supplementations in simulated gastric fluid (SGF).
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Nanopartículas Metálicas , beta-Ciclodextrinas , Carbono , Nitrogênio , Compostos Férricos , Prata , Ferro , Compostos FerrososRESUMO
Based on the dual-resonance principle around the dispersion turning point, a scheme of chiral long-period fiber gratings (CLPGs) formed by twisting a high-birefringence (Hi-Bi) fiber is herein proposed to realise ultra-broadband flat-top circular polarizers. The coupling bandwidth is approximately seven times larger than that of traditional CLPGs. In addition, by introducing chirp characteristics in these CLPGs, an ultra-broadband flat-top circular polarizer with â¼200â nm@3â dB was conveniently achieved. Subsequently, by optimising the chirped CLPGs, a circular polarizer with a bandwidth extinction ratio of approximately 30â dB and a high level of â¼100â nm at 1â dB was realised. It was shown that the mode-controlling performances of the CLPGs can be remarkably improved, which has significant applications in light-field regulation. Finally, for the first time, it was proved that the CLPG cannot generate a vortex beam.
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Imaging in visible and short-wave infrared (SWIR) wavebands is essential in most remote sensing applications. However, compared to visible imaging cameras, SWIR cameras typically have lower spatial resolution, which limits the detailed information shown in SWIR images. We propose a method to reconstruct high-resolution polarization SWIR images with the help of color images using the deep learning method. The training dataset is constructed from color images, and the trained model is well suited for SWIR image reconstruction. The experimental results show the effectiveness of the proposed method in enhancing the quality of the polarized SWIR images with much better spatial resolution. Some buried spatial and polarized information may be recovered in the reconstructed SWIR images.
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Processamento de Imagem Assistida por Computador , Redes Neurais de Computação , Processamento de Imagem Assistida por Computador/métodos , Análise EspectralRESUMO
Polarimetric dehazing method is very promising in enhancing the quality of images captured in the scattering media. However, it is found that the dehazing results calculated by hazy images are very sensitive to the noise, which may cause the method unstable or even invalid. To overcome this drawback and enhance the capability and stability of the polarimetric dehazing method, digital image processing algorithms or bias parameters need to be added into the method, however, they will make the algorithm complex and time consuming. In this paper, using low pass filter to suppress the noise of the hazy images, a novel polarimetric dehazing method is proposed to enhance the visibility of hazy images, especially for dense haze removal. Experimental results demonstrate that this method is totally automatic and very effective in dense haze processing. This method may have great potential usage in many applications, such as optical surveillance, underwater imaging, and bio-tissue imaging, etc.
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Pectin is a kind of natural and complex carbohydrates which is extensively used in food, chemical, cosmetic, and pharmaceutical industries. Fresh sunflower (Helianthus annuus L.) heads were utilized as a novel source of pectin extracted by ammonium oxalate. The conditions of the extraction process were optimized implementing the response surface methodology. Under optimal extraction parameters (extraction time 1.34 h, liquid-solid ratio 15:1 mL/g, ammonium oxalate concentration 0.76% (w/v)), the maximum experimental yield was 7.36%. The effect of spray-drying and freeze-drying on the physiochemical properties, structural characteristics, and antioxidant activities was investigated by FT-IR spectroscopy, high performance size exclusion chromatography, and X-ray diffraction. The results showed freeze-drying lead to decrease in galacturonic acid (GalA) content (76.2%), molecular weight (Mw 316 kDa), and crystallinity. The antioxidant activities of pectin were investigated utilizing the in-vitro DPPH and ABTS radical-scavenging systems. This study provided a novel and efficient extraction method of sunflower pectin, and confirmed that different drying processes had an effect on the structure and properties of pectin.
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A novel method to control the parameters of a chiral fiber grating structure is proposed. Mode couplings are controlled in real time during the twisting fabrication process. This chiral grating structure can satisfy the phase-matching condition for generating high-quality orbital angular momentum (OAM) beams, with an order mode of conversion efficiency over 99.9%. Both theoretical analysis and experimental results of this OAM mode conversion have been investigated, with good agreement. The results demonstrate a dual-OAM beam converter with a charge of ±1 for the right- and left-handed CLPGs, respectively. The high-quality OAM beam generated in this twisted single-mode fiber process may find excellent applications in optical communications.
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Polarimetric dehazing methods can significantly enhance the quality of hazy images. However, current methods are not robust enough under different imaging conditions. In this paper, we propose a generalized polarimetric dehazing method based on low-pass filtering in the frequency domain. This method can accurately estimate the polarized state of the scattering light automatically without adjusting bias parameters. Experimental results show the effectiveness and robustness of our proposed method in different hazy weather and scattering underwater environments with different densities. Furthermore, computational efficiency is enhanced more than 70% compared to the polarimetric dehazing method we proposed previously.
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Based on a parallel phase compensation scheme, we propose an efficient wavefront shaping method using a spatial light modulator (SLM) for quickly generating a series of focused spots through a multimode fiber (MMF). The compensated phase mask obtained by a two-step phase-shifting technique is loaded to the SLM for generating a focused spot at an arbitrary target position out of the fiber facet. Furthermore, the parallel algorithm we present makes it possible to obtain a series of compensated phase masks, which could be used to generate a series of focused spots at different locations. We experimentally obtained 100 tightly focused spots, with an average focused efficiency of 21.60% and an average focused diameter of 1.9240 µm, and only one-time parallel-compensated phase retrieval is required without multiple iteration optimization.
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In this Letter, we propose a simple, low-cost, reconfigurable snapshot polarimetric imaging technique for a color camera to measure polarization properties with spectral-polarization filters. Experimental results demonstrate the unique capabilities, such as obtaining circular or elliptical polarized information in a snapshot, that are not available from commercial polarization cameras and other polarization imaging techniques.
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In this paper, a two-step optical design method is proposed to build a superior Fresnel-based photovoltaic concentrator for enhancing light conversion efficiency with the multi-junction solar cell. In the first step, we orthogonally segment a traditional Fresnel concentrator and remove the two normal stripe bands around the horizontal and vertical axes, as well as recombine the resultant four quadrants again. By using such a specific Fresnel concentrator design, a square light pattern can be constructed owing to the off-axis non-rotational symmetric superposition of light. In the second step, as for the response wavelengths of a specific triple-junction solar cell, we further carry out a triple-wavelength and multi-focus design of the above Fresnel concentrator for improving the uniformity of light distribution on the solar cell. To show the validity of this novel design method, a solar concentrator, with typical design parameters including the geometrical concentration ratio of 800× and the F-number of 0.775, is designed and simulated. As a result, theoretical irradiance uniformity up to 87% is obtained. In addition, considering the fact that no second optical element is involved in the concentrator system, our design method inherently has the advantages of good compactness, high efficiency, low cost, and ease for mass-production. We believe that such a concentrator is of great significance to solar cells for high conversion efficiency of light in the concentrator photovoltaic system.
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Shortwave infrared (SWIR) polarimetric imaging has been found very effective in various applications. However, the low resolution of the SWIR camera severely limits the capacity of this technique. Image reconstruction methods have been developed to improve the spatial resolution, but these methods typically do not consider the polarized information that the images may contain. In this paper, we propose a high-resolution reconstruction method for SWIR images based on the spatial information of visible images without losing polarized information in the SWIR image. Experimental results demonstrate that this method is feasible to reconstruct high-resolution polarized SWIR images. We have also demonstrated its potential application in image fusion.
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In this paper, we report a nonscanning optical superresolution imaging method based on a hybrid phase-amplitude superoscillation element. Using the Chebyshev polynomials as a basis set on the superoscillation waveform, the optimal combination of these, representing the optimal focal -spot in the local field of view, is found by genetic algorithm. Our numerical calculations demonstrate that a subwavelength focal spot with a full width at half-maximum as small as 253 nm is realized, which has more than 30 times improvement in sidelobe suppression ratio, and crucially, a greatly extended needle with continuously shrunken focal spot is yielded, which allows a large imaging tolerance in the axial displacement of the object. We then present our simulated results of the superresolution imaging on sparse point objects and continuous objects, where the practicality and effectiveness of this method are analyzed and discussed in detail.
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Suffering from the limited number aperture, a resolution higher than 0.5λ cannot be obtained in conventional far-field optical imaging systems. Recently, research of the optical super-oscillation lens (SOL) has been demonstrated to realize sub-diffractive focusing in both theory and practice successfully. Nevertheless, these systems usually have characteristics of a localized field of view and low-intensity focal spot surrounded by huge sidelobes, which profoundly restricts their application for super-resolution imaging. In this paper, we artificially segment the SOL into two simple-fabrication portions to generate the super-oscillation optical field and realize off-axis far-field imaging. Meanwhile, the point spread function (PSF) feature extraction algorithm is proposed to break the limitation of low core intensity of the PSF and then effectively extract the sub-diffractive structure annihilated by huge sidelobes. Simulations demonstrate its feasibility and reliability in sub-diffraction information recovery, and targets with a resolution of 250 nm are well recovered in the super-oscillation area.
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In this paper, we propose a microfiber-plane-grating composite optical waveguide (MPGCOW), which is formed by immobilizing a tapered microfiber on the surface of a plane grating with one defect, for gas refractive index (RI) sensing. Its optical properties and gas RI sensing properties are investigated by the finite difference time domain method. Results show that the MPGCOW has a photonic stop band and is very sensitive to the ambient gas RI variation. The largest gas RI sensing sensitivity of 486.67 nm/RIU and detection limit of 2×10-6 are obtained by immersing the structure in the mixture gas of N2 and He with various mixture ratios.
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A promising technology for fabricating chiral long-period gratings (CLPGs) is demonstrated using a commercial fusion splicer. The key aspect of this technology is the incorporation of a fully automatic program we designed for the fusion splicer. High-quality CLPGs are successfully fabricated from single-mode fibers, which have very flat surfaces and low insertion loss. We also investigate the tuning characteristics of the transmission spectrum with the mechanical twist rate in CLPGs for torsion sensing application. The torsion sensitivity is improved and the shift in resonance wavelength versus the mechanical twist rate shows an almost perfect linear relationship. In addition, by choosing appropriate fabrication parameters, the fabricated CLPGs can be used as tunable single-band-rejection filters in a broad wavelength range.
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Polarimetric dehazing methods have been proven to be effective in enhancing the quality of images acquired in turbid media. We report a new full-Stokes polarimetric camera, which is based on the division of aperture structure. We design a kind of automatic polarimetric dehazing algorithm and load it into the field programmable gate array (FPGA) modules of our designed polarimetric camera, achieving a real-time image haze removal with an output rate of 25 fps. We demonstrate that the image quality can be significantly improved together with a good color restoration. This technique might be attractive in a range of real-time outdoor imaging applications, such as navigation, monitoring, and remote sensing.
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We present a novel online fabrication scheme of helical long-period fiber gratings (H-LPFGs) by directly twisting a standard single-mode fiber (SMF) in a microheater. This is done by taking advantage of the inherent core-cladding eccentricity in SMF. We adopt a fiber optic rotary joint to eliminate the accompanying twisting spiral for real-time spectral monitoring and a stepping mechanical system to accurately control the twisting length in fabrication. As a consequence, low-cost and high-quality H-LPFGs can be readily fabricated. Meanwhile, by using this kind of H-LPFG, we design a simple and low-cost wavelength-interrogated liquid-level sensor with a high sensitivity of 0.1 nm/mm.
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Polarimetric dehazing methods have proven effective in enhancing the quality of chromatic hazy images. Considering that the infrared radiance has a better capacity for traveling through the haze, in this paper we propose a polarimetric dehazing method based on visible and infrared image fusion to improve the visibility of hazy images, especially for dense haze conditions. Experimental results demonstrate that the visibility of hazy images can be effectively enhanced, and the color information can be finely maintained. The visibility of dehazed images can be promoted at least 100%. This kind of dehazing method can be used widely in many dehazing applications.
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Based on the theoretical model of a microfiber double-knot resonator with a parallel structure, numerical simulations on the transmission spectrum, the phase, and the group time delay of the resonator as a function of wavelengths are given. We find that with this kind of resonator both slow light and fast light can be obtained at different resonant wavelengths. Experimentally, such a kind of microfiber resonator was fabricated successfully. The transmission spectrum of the fabricated resonator is well consistent with the theoretical simulation. A slow-light delay of about 38 ps and a fast-light advance of about 40 ps are demonstrated at different wavelengths, which might benefit the resonator to the applications in data delay lines, optical buffers, and optical memories.