RESUMO
Enhancement of the environmentally responsive fluorescent properties of polydiacetylene (PDA) by combination with lipophilic fluorophores was demonstrated and properties of the PDA/fluorophore systems were explored. Liposomes containing PDA and fluorophores exhibited enhanced Stokes shift and increase in emission as a result of energy transfer from PDA to fluorophore. The effects of fluorophore variation, degree of PDA polymerization and diyne placement in the diacetylene lipid tails on the emission enhancement were studied. It was determined that signal generation was optimized at a relatively low extent of PDA polymerization with the optimal degree of polymerization dependent on the other parameters. Energy transfer was used as a tool to detect fluorophore exchange between polymerized and unpolymerized liposomes and to study the effects of fluorophore structure on exchange from unpolymerized to PDA liposomes. Fluorophores that locate at the aqueous interface with alkyl anchors were slow to transfer while fluorophores that partition into the alkyl regions of the liposomes transfer quickly.
Assuntos
Técnicas Biossensoriais , Compostos de Boro/química , Carbocianinas/química , Corantes Fluorescentes/química , Polímeros/química , Poli-Inos/química , Transferência de Energia , Lipossomos , Modelos Moleculares , Polímero Poliacetilênico , Espectrometria de FluorescênciaRESUMO
Polydiacetylene (PDA) materials are used as a platform for detection of biological analytes such as microorganisms, viruses and proteins. The environmentally responsive chromic and emissive properties of the polymer, combined with self-assembled material formats, make these materials particularly attractive for biosensing applications. A variety of approaches have been used in developing these materials and demonstrating their potential for biological detection. In this feature article we describe different PDA material formats, discuss the optical properties that are the basis for signal generation, and review the use of PDA for biosensing.
Assuntos
Técnicas Biossensoriais , Polímeros/química , Poli-Inos/química , Algoritmos , Bactérias/química , Coloides/química , Cor , Lipossomos , Proteínas de Membrana/química , Membranas Artificiais , Óptica e Fotônica , Polímero Poliacetilênico , Proteínas/química , Vírus/químicaRESUMO
The preparation and characterization of coatings made from polydiacetylene colloids on nano- and microporous membranes and their potential for the detection of microorganisms are presented.