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1.
J Bodyw Mov Ther ; 35: 273-283, 2023 07.
Artigo em Inglês | MEDLINE | ID: mdl-37330781

RESUMO

BACKGROUND: This systematic review and meta-analysis examined the effects of foam roller or stick massage performed after exercise-induced muscle damage protocols on indirect markers of muscle damage compared to a non-intervention control group in healthy individuals. METHODS: PubMed, Biblioteca Virtual em Saúde, Scopus, Google Scholar, and Cochrane Library database were searched in August 2, 2020, with last update on February 21, 2021. Were included clinical trials involving healthy adult individuals who received foam roller/stick massage versus a non-intervention group and evaluated indirect markers of muscle damage. Risk of bias was assessed by the Cochrane Risk of Bias tools. Standardized mean differences with 95% confidence intervals were used to measure the foam roller/stick massage effect on muscle soreness. RESULTS: The five included studies investigated 151 participants (136 men). Overall, the studies presented a moderate/high risk of bias. A between-groups meta-analysis showed no significant difference between massage and non-intervention control groups on muscle soreness immediately after (0.26 [95%CI: 0.14; 0.65], p = 0.20), 24 h (-0.64 [95%CI: 1.34; 0.07], p = 0.08), 48 h (-0.35 [95%CI: 0.85; 0.15], p = 0.17), 72 h (-0.40 [95%CI: 0.92; 0.12], p = 0.13), and 96 h (0.05 [95%CI: 0.40; 0.50], p = 0.82) after an exercise-induced muscle damage protocol. Moreover, the qualitative synthesis showed that foam roller or stick massage had no significant effect on range of motion, muscle swelling, and maximal voluntary isometric contraction recovery. CONCLUSION: In conclusion, the current literature appears to not support the advantage of foam roller or stick massage to improve recovery of muscle damage indirect markers (muscle soreness, range of motion, muscle swelling, and maximal voluntary isometric contraction) compared to a non-intervention control group in healthy individuals. Furthermore, due to the heterogeneity of the methodological designs among the included studies, making it difficult to compare the results. In addition, there are not enough high-quality and well-designed studies on foam roller or stick massage to draw any definite conclusions. REVIEW PROTOCOL NUMBER: The study was pre-registered in the International Prospective Register of Systematic Review (PROSPERO) on August 2, 2020, with last update on February 21, 2021. Protocol number: CRD2017058559.


Assuntos
Músculo Esquelético , Mialgia , Masculino , Adulto , Humanos , Mialgia/etiologia , Mialgia/terapia , Músculo Esquelético/fisiologia , Amplitude de Movimento Articular/fisiologia , Contração Isométrica , Massagem/métodos
2.
J Microbiol Methods ; 205: 106674, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36641085

RESUMO

Isothermal microcalorimetry can provide a general analytical tool for the characterization of bacterial growth. Methodologies and equipment have been studied to expand the application and disseminate the use of the technique. The MCDSC is a microcalorimeter capable of measuring in the range of 0.2 µW that can operate at a temperature range of -20 to 140 °C or under isothermal conditions. Here, we present the first investigation of MCDSC for E. coli growth with the Baranyi and Roberts modeling application. This study presented the calorimetric E. coli fingerprint at MCDSC and compares it with the plate count technique, giving the data more biological meaning. The calorimeter was able to accurately detect growth metabolism and discriminate E. coli at different inoculum densities. Additionally, the MCDSC can offer a new point of view for evaluating microbial growth, such as the significant reduction in error due to dispersed data by the viable counting method.


Assuntos
Escherichia coli , Calorimetria/métodos , Temperatura , Contagem de Colônia Microbiana
3.
J Food Prot ; 85(11): 1566-1575, 2022 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-35202475

RESUMO

ABSTRACT: The monitoring of carcass surface contamination along the slaughter line enables verification of slaughter operation hygiene and the use of good manufacturing practices. Pork meat is a common source of human nontyphoidal salmonellosis, one of the most frequently reported foodborne illnesses worldwide. This study was conducted to gather data on microbial loads before and after evisceration on the surfaces of swine carcasses in two slaughterhouses. The presence of Salmonella enterica was evaluated only after evisceration on carcass surfaces and in livers and floor drains (environmental samples) because pigs are common carriers of this pathogen. The contamination of carcass surfaces was evaluated by delimitation of surface area with sterilized templates (100 cm2), and surface samples were collected with gauze swabs. Total aerobic mesophilic bacteria, Enterobacteriaceae, and Escherichia coli were enumerated. Significant differences (P < 0.05) in counts of mesophilic bacteria, Enterobacteriaceae, and E. coli were found on the external carcass surfaces, with higher counts after evisceration. The neck and abdominal areas had higher levels of mesophilic bacteria, Enterobacteriaceae, and E. coli and a high prevalence of Salmonella. Salmonella was detected in only one of the studied slaughterhouses; 19 (7.3%) of 259 analyzed carcass samples were positive for Salmonella, and Salmonella was detected in two livers and two floor drains. A total of 52 Salmonella isolates (44 from carcasses, 5 from livers, and 3 from drains) were recovered. Three Salmonella serovars (Typhimurium 4,5:i- , Wernigerone, and Derby) were identified, and 53.8% of the 52 isolates were multidrug resistant. The results reveal the need for continuous improvement of slaughtering operations and implementation of good manufacturing practices to ensure the safety of pork produced in Portugal.


Assuntos
Matadouros , Escherichia coli , Suínos , Humanos , Animais , Portugal , Carne/microbiologia , Salmonella , Enterobacteriaceae , Higiene , Contaminação de Alimentos/análise , Prevalência
4.
J Bodyw Mov Ther ; 28: 121-125, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34776128

RESUMO

BACKGROUND: Myofascial release (MR) has been widely used in sports and clinical environment. There are studies that have already evaluated the effect of MR on postural control, however, the data found are inconclusive. OBJECTIVES: Investigate the effect of MR on ankle plantar flexor muscles over static postural balance. METHOD: Sixty-five young men were randomized allocated into one of three groups: 1) MR group (n = 25, Myofascial Release); 2) Sham (n = 25, fake intervention); or 3) Control group (n = 15). Both MR and Sham techniques were applied during 5 min in the ankle flexors of each leg. The bipodal static postural balance test was performed before and 2 min post each intervention on a force plate with eyes closed during the test. All subjects performed 3 sets of 30-s with 1 min of rest. Mean values to the center of pressure velocity (COPvel) was used for analysis. RESULTS: The bipodal balance test showed that there was no significant difference between groups (p > 0.05), but there was a significant time effect (p < 0.05). A significant decrease (p < 0.05) in post-test COPvel (cm.s-1) values (sham 4.9 ± 1.2; MR 4.5 ± 0.6; CON 4.22 ± 0.8) was found when compared to values of pre-test (sham 5.3 ± 1.0; MR 5.0 ± 0.8; CON 4.94 ± 1.3) for all groups. CONCLUSIONS: The results demonstrated that MR performed on ankle flexors did not affect static postural balance in young man. This study is a clinical trial (Register Number: RBR-48k5jm).


Assuntos
Traumatismos do Tornozelo , Tornozelo , Articulação do Tornozelo , Humanos , Masculino , Terapia de Liberação Miofascial , Equilíbrio Postural
5.
Rev Gaucha Enferm ; 41: e20190419, 2020.
Artigo em Inglês, Português | MEDLINE | ID: mdl-33237223

RESUMO

OBJECTIVE: To know the perception of multiparous women about their experiences with obstetric violence. METHODS: Qualitative descriptive study carried out from January to May 2019 in basic health units in the city of Rio Grande, Rio Grande do Sul. Twenty multiparous women from the community participated in the study. Data were collected through interviews and submitted to content analysis. RESULTS: Two subcategories were constructed: Obstetric Violence in primiparous women, where women suffered verbal violence to collaborate during fetal expulsion in labor; Obstetric violence in multiparous women, where there was verbal and physical violence related to the fact that the women had many children. FINAL CONSIDERATIONS: Obstetric violence in health institutions is experienced by many women. The trauma suffered will follow them through their lives. The naturalization of violent practices during labor and birth should be avoided, in order to ensure respectful and non-discriminatory care.


Assuntos
Trabalho de Parto , Parto , Criança , Feminino , Humanos , Paridade , Percepção , Gravidez , Violência
6.
J Exerc Sci Fit ; 18(3): 148-154, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32514278

RESUMO

BACKGROUND/OBJECTIVE: Roller massage has become a popular intervention in sports settings in order to treat muscle soreness and stiffness, as well as improving post-exercise recovery, although there is limited evidence for these assumptions. Therefore, the purpose of this study was to evaluate the effects of a single session of roller massage, applied with a controlled force after an exercise-induced muscle damage protocol, on muscle recovery. METHODS: A randomized controlled study was performed using a repeated-measures design. Thirty-six young men completed four sets of six eccentric actions of elbow flexors at 90°/s with a 90s rest interval between sets. Participants were randomly assigned into one of three groups: 1) Roller massage (n = 12), 2) Sham (n = 12), and 3) Control (n = 12). Maximal isometric voluntary contraction (MIVC), delayed-onset muscle soreness (DOMS), range of motion (ROM), and muscle thickness were measured at baseline, and at 24, 48, and 72 h post exercise. RESULTS: There was no significant group by time interaction for MIVC (p = 0.090) and ROM (p = 0.416). Also, although there was a significant group by time interaction for muscle thickness (p = 0.028), post hoc test did not find significant difference between groups (p > 0.05). DOMS was recovered at 72 h for roller massage (p < 0.001) and control (p < 0.001) groups, while the Sham group did not recover from DOMS across 72 h (p < 0.001). There was also no significant difference between groups in DOMS at any time (p > 0.05). CONCLUSIONS: A single session of roller massage applied on elbow flexors had no effect on recovery of MIVC, muscle swelling, ROM and DOMS.

7.
Rev. gaúch. enferm ; 41: e20190419, 2020.
Artigo em Inglês | LILACS, BDENF - Enfermagem | ID: biblio-1139139

RESUMO

ABSTRACT Objective: To know the perception of multiparous women about their experiences with obstetric violence. Methods: Qualitative descriptive study carried out from January to May 2019 in basic health units in the city of Rio Grande, Rio Grande do Sul. Twenty multiparous women from the community participated in the study. Data were collected through interviews and submitted to content analysis. Results: Two subcategories were constructed: Obstetric Violence in primiparous women, where women suffered verbal violence to collaborate during fetal expulsion in labor; Obstetric violence in multiparous women, where there was verbal and physical violence related to the fact that the women had many children. Final considerations: Obstetric violence in health institutions is experienced by many women. The trauma suffered will follow them through their lives. The naturalization of violent practices during labor and birth should be avoided, in order to ensure respectful and non-discriminatory care.


RESUMEN Objetivo: Conocer la percepción de las mujeres multíparas sobre sus experiencias con la violencia obstétrica. Métodos: Estudio descriptivo cualitativo llevado a cabo de enero a mayo de 2019 en unidades básicas de salud en la ciudad de Rio Grande, Rio Grande do Sul. Participaron veinte mujeres multíparas de la comunidad. Los datos fueron recolectados a través de entrevistas y sometidos a análisis de contenido. Resultados: Se construyeron dos subcategorías: violencia obstétrica cuando era primípara, donde las mujeres sufrían violencia verbal para colaborar con el período expulsivo del parto; Violencia obstétrica cuando es multípara, donde se observó violencia verbal y física, guiada por la gran cantidad de niños que tuvieron. Consideraciones finales: La violencia obstétrica en las instituciones de salud es un hecho experimentado por muchas mujeres. El trauma sufrido los acompaña a lo largo de sus vidas. Es necesario evitar la naturalización de las prácticas violentas durante el proceso de nacimiento / parto, garantizando una atención respetuosa y no discriminatoria.


RESUMO Objetivo: Conhecer a percepção das multíparas acerca das suas experiências com a violência obstétrica. Métodos: Estudo qualitativo descritivo realizado de janeiro a maio de 2019 nas unidades básicas de saúde do município de Rio Grande, Rio Grande do Sul. Participaram 20 mulheres multíparas da comunidade. Os dados foram coletados por entrevistas e submetidos à análise de conteúdo. Resultados: Foram construídas duas subcategorias: Violência Obstétrica quando primíparas, onde as mulheres sofriam violência verbal para colaborarem com o período expulsivo do parto; Violência Obstétrica quando multíparas, onde foi observada violência verbal e física, pautadas pelo grande número de filhos que possuíam. Considerações finais: A violência obstétrica nas instituições de saúde é fato vivenciado por muitas mulheres. O trauma sofrido as acompanha ao longo da vida. É preciso evitar a naturalização de práticas violentas durante o processo de parto/nascimento garantindo um cuidado respeitoso e sem discriminação.


Assuntos
Criança , Feminino , Humanos , Gravidez , Trabalho de Parto , Parto , Paridade , Percepção , Violência
8.
Rev. Inst. Adolfo Lutz ; 75: 01-08, 2016. tab, graf
Artigo em Português | LILACS, VETINDEX | ID: biblio-1489537

RESUMO

Nos últimos anos, o estilo de vida tem trazido mudanças na alimentação da população, com o aumento de refeições feitas fora do domicílio, da procura pela praticidade de alimentos prontos e também da busca por uma alimentação mais saudável. O controle da produção e comercialização desses produtos deve ser rigoroso para proteger a saúde da população. Nas praias, observa-se que a estrutura dos quiosques não é adequada para a manipulação de certos alimentos, por isso a venda de salada de frutas não é permitida nas praias de Florianópolis - SC, apesar de ser amplamente comercializada. O objetivo deste trabalho foi de avaliar a qualidade higiênico-sanitária, quanto à presença de Salmonella e à enumeração de coliformes termotolerantes, em 40 amostras de saladas de frutas comercializadas em quiosques de quatro praias de Florianópolis. Os resultados foram comparados com os padrões estabelecidos pela RDC nº 12/01. Somente 5 % das amostras estavam impróprias para o consumo humano por extrapolarem o limite de coliformes termotolerantes. Todas as amostras apresentaram ausência de Salmonella em 25 g. O resultado microbiológico satisfatório da maioria das amostras pode estar associado às condições intrínsecas da salada de frutas, que possui pH baixo o suficiente para inibir o crescimento dos micro-organismos pesquisados.


Recently, changes in lifestyle have modified the eating habits, with an increase in having meals outside of home and demanding for the convenience of ready-to-eat foods, and also rising the search for healthier diet. Controls on this kind of food production and commercialization should be strict for protecting the population health. Beach kiosks do not offer the suitable conditions for handling certain foods due to their lack of structure. Therefore, it is prohibited to sell fruit salads on the beaches of Florianopolis-SC, although they have been amply commercialized. Hygiene and sanitation conditions were evaluated in 40 fruit salad samples sold in kiosks on the four beaches in Florianopolis, by analyzing the occurrence of Salmonella and thermotolerant coliforms counting. These results were compared to the standards established by the local regulation (RDC 12/01). Only 5 % of the samples were considered unsuitable for human consumption, as they exceeded the limits established for thermotolerant coliform. All of the samples showed negative results for Salmonella in 25 g of food. The satisfactory microbiological results found in this study might be associated with the intrinsic characteristics of fruit salads, which possess a low pH value and being enough to inhibit the growth of these two microorganisms.


Assuntos
Coliformes , Frutas , Higiene dos Alimentos , Inspeção de Alimentos , Salmonella , Colimetria , Comercialização de Produtos , Inocuidade dos Alimentos , Padrão de Identidade e Qualidade para Produtos e Serviços
9.
Rev. Inst. Adolfo Lutz (Online) ; 75: 1-8, 2016. tab, graf
Artigo em Português | LILACS, Sec. Est. Saúde SP, SESSP-ACVSES, SESSP-IALPROD, Sec. Est. Saúde SP, SESSP-IALACERVO | ID: biblio-982793

RESUMO

Nos últimos anos, o estilo de vida tem trazido mudanças na alimentação da população, com o aumento de refeições feitas fora do domicílio, da procura pela praticidade de alimentos prontos e também da busca por uma alimentação mais saudável. O controle da produção e comercialização desses produtos deve ser rigoroso para proteger a saúde da população. Nas praias, observa-se que a estrutura dos quiosques não é adequada para a manipulação de certos alimentos, por isso a venda de salada de frutas não é permitida nas praias de Florianópolis - SC, apesar de ser amplamente comercializada. O objetivo deste trabalho foi de avaliar a qualidade higiênico-sanitária, quanto à presença de Salmonella e à enumeração de coliformes termotolerantes, em 40 amostras de saladas de frutas comercializadas em quiosques de quatro praias de Florianópolis. Os resultados foram comparados com os padrões estabelecidos pela RDC nº 12/01. Somente 5 % das amostras estavam impróprias para o consumo humano por extrapolarem o limite de coliformes termotolerantes. Todas as amostras apresentaram ausência de Salmonella em 25 g. O resultado microbiológico satisfatório da maioria das amostras pode estar associado às condições intrínsecas da salada de frutas, que possui pH baixo o suficiente para inibir o crescimento dos micro-organismos pesquisados.


Recently, changes in lifestyle have modified the eating habits, with an increase in having mealsoutside of home and demanding for the convenience of ready-to-eat foods, and also risingthe search for healthier diet. Controls on this kind of food production and commercializationshould be strict for protecting the population health. Beach kiosks do not offer the suitableconditions for handling certain foods due to their lack of structure. Therefore, it is prohibited to sellfruit salads on the beaches of Florianopolis-SC, although they have been amply commercialized.Hygiene and sanitation conditions were evaluated in 40 fruit salad samples sold in kiosks on thefour beaches in Florianopolis, by analyzing the occurrence of Salmonella and thermotolerantcoliforms counting. These results were compared to the standards established by the localregulation (RDC 12/01). Only 5 % of the samples were considered unsuitable for humanconsumption, as they exceeded the limits established for thermotolerant coliform. All of the samplesshowed negative results for Salmonella in 25 g of food. The satisfactory microbiological resultsfound in this study might be associated with the intrinsic characteristics of fruit salads, which possessa low pH value and being enough to inhibit the growth of these two microorganisms.


Assuntos
Humanos , Coliformes , Qualidade dos Alimentos , Abastecimento de Alimentos , Frutas , Salmonella , Alimentos de Rua
10.
Food Chem ; 172: 725-30, 2015 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-25442614

RESUMO

Fast and efficient analytical methods to determine the concentrations of lactic, acetic, propionic and butyric acids in sour cassava starch wastewater using reversed-phase high performance liquid chromatography (HPLC) and capillary electrophoresis (CE), were developed and validated. Good linearity (R(2) > 0.999) and significance with F > 25,000 for all acids was showed. The matrix effect was not detected. The coefficient of variation values indicated good repeatability. The limits of detection (LOD) ranged from 1.0 to 3.7 and 2.0 to 3.0, and the limits of quantification (LOQ) from 3.1 to 12.2, and 8.0 to 12.5mg/L for HPLC and CE, respectively. The quantification of the samples did not reveal significant differences between the methods for all compounds analyzed. However, the benefits of CE in relation to HPLC, such as lower costs and less waste generation, along with shorter analysis times, need to be taken into consideration.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Eletroforese Capilar/métodos , Manihot/química , Compostos Orgânicos/análise , Amido/química , Águas Residuárias/análise , Fermentação
11.
Int J Mol Sci ; 11(4): 1944-55, 2010 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-20480051

RESUMO

The present study evaluated the effect of aflatoxin B(1) (AFB(1)) and fumonisin B(1) (FB(1)) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB(1) and FB(1) used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner.


Assuntos
Aflatoxina B1/toxicidade , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Fumonisinas/toxicidade , Hepatócitos/efeitos dos fármacos , Animais , Células Cultivadas , Fragmentação do DNA/efeitos dos fármacos , Hepatócitos/citologia , Masculino , Microscopia Confocal , Ratos , Ratos Wistar
12.
Ciênc. agrotec., (Impr.) ; 32(1): 225-230, jan.-fev. 2008. graf, tab
Artigo em Português | LILACS | ID: lil-479122

RESUMO

As propriedades físicas dos produtos agrícolas são influenciadas diretamente pela secagem. Assim, buscou-se com o presente trabalho avaliar o efeito do teor de água nas propriedades físicas dos grãos de feijão da cultivar Vermelho Coimbra, e ajustar diferentes modelos matemáticos aos valores experimentais. Analisaram-se duas metodologias para a determinação da massa específica aparente e da massa específica unitária. A massa específica aparente e a porosidade da massa foram determinadas utilizando-se uma balança de peso hectolitro e um picnômetro de comparação a ar, respectivamente. A massa específica unitária foi determinada em função da porosidade e da massa específica aparente. Os dados experimentais da massa específica aparente e unitária foram comparados com os obtidos pelo cálculo a partir da composição química média dos grãos de feijão. Concluiu-se que a redução no teor de água pela secagem influencia as propriedades físicas dos grãos, proporcionando a diminuição da porosidade e o aumento das massas específicas aparente e da massa específica unitária. A metodologia de determinação das massas específicas pela composição química média dos grãos de feijão apresenta limitação quanto à precisão dos resultados. As variações das propriedades físicas analisadas em função do teor de água são adequadamente representadas pelo modelo linear.


The physical properties of the agricultural products are influenced directly by drying. Like this, the objective of this work was to evaluate the effect of moisture content variation in the physical properties of edible bean grain Vermelho Coimbra cultivar, and to fit different mathematical models to the experimental values. Two methodologies were analyzed for the determination of the bulk density and true density. The bulk density and the porosity were determined being used a hectoliter balance and a picnometer, respectively. The true density was determined in function of the porosity and the bulk density. The experimental data of the bulk density and true density were compared to the obtained data by the calculation starting from the chemical composition of the edible beans. It was concluded that the moisture content reduction affects the the physical properties of edible bean grain, providing the decrease of the porosity and of the mass of 1000 grains and the increase of bulk density and true density. The methodology of determination of the bulk density and true density using the chemical composition of the bean grains presents limitation with relationship to the precision of the results. The variations of the physical properties analyzed in function of the moisture content are better fitted represented by the lineal model.

13.
Ciênc. agrotec., (Impr.) ; 31(2): 462-467, mar.-abr. 2007. tab, graf
Artigo em Português | LILACS | ID: lil-454372

RESUMO

Objetivou-se com o presente trabalho, avaliar as propriedades térmicas dos grãos de trigo, de uma variedade nacional (Aliança), durante o processo de secagem em estufa e ajustar modelos matemáticos que representem satisfatoriamente as variáveis estudadas em função do teor de água. Foram utilizados grãos de trigo com teor de água inicial de 26 por centob.s. secos até o teor final de 11 por centob.s. em estufa com ventilação forçada a 40 °C e umidade relativa de 45 por cento. As variáveis estudadas foram o calor específico, obtido pelo método das misturas; a condutividade térmica, obtida por meio de um cilindro teoricamente infinito considerando a hipótese da condução unidimensional na direção radial; e a difusividade térmica, determinada indiretamente utilizando-se os valores experimentais da massa específica aparente, calor específico e condutividade térmica do trigo. Com base nos resultados obtidos, pode-se concluir que o calor específico e a condutividade térmica do trigo aumentam, enquanto a difusividade térmica apresenta redução dos seus valores com a elevação do teor de água e que estas propriedades térmicas do trigo variam linearmente com o teor de água, sendo satisfatoriamente representadas pelo modelo linear simples, para a faixa de umidade estudada.


The objective of this work was to evaluate the thermal properties of wheat grain, of a national variety, during the drying process in oven and to adjust mathematical models which represent satisfactorily these variables. Wheat grains with initial moisture content of 26 percentd.b. were dried in air forced oven up to 11 percentd.b. as the final moisture content. The specific heat was obtained by the method of the mixtures and the thermal conductivity by the utilization of a theoretically infinite cylinder considering the hypothesis of the conduction unidimensional in the radial direction. For thermal diffusivity was certain indirectly using itself the experimental values of the bulk density, specific heat and thermal conductivity of the wheat. Based on the results, it can be concluded that moisture content reduction promotes the specific heat and the thermal conductivity reduction but thermal diffusivity increase and which these thermal properties of the wheat vary lineally with water content, for the interval of studied humidity.

14.
Can J Microbiol ; 52(8): 798-804, 2006 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16917539

RESUMO

Fusarium verticillioides (Gibberella fujikuroi mating population A) is a producer of fumonisins and one of the main contaminants of corn grains. In Brazil, some studies analyzing strains isolated from corn have demonstrated high levels of fumonisins, whereas the levels for strains isolated from sorghum have been found to be low. In the present study, we investigated the genetic diversity of 22 F. verticillioides strains isolated from corn and 21 strains isolated from sorghum cultivated in the State of São Paulo, Brazil. Differences in the genetic profile were observed between the strains isolated from the two substrates using single primer amplification reaction by polymerase chain reaction (SPAR-PCR). Fumonisins levels were higher in strains isolated from corn than in those isolated from sorghum. The MAT-1 and MAT-2 alleles were identified by PCR, and the isolates were subsequently crossed with Fusarium thapsinum (G. fujikuroi mating population F) reference strains because this species is morphologically similar to F. verticillioides and produces low levels of fumonisins. The SPAR haplotypes of some strains isolated from sorghum were similar to the F. thapsinum reference strain haplotypes, but there was no fertile mating between the strains isolated from the two substrates and the F. thapsinum references strains. The MAT-1:MAT-2 proportion was 5:17 and 14:7 for isolates from corn and sorghum, respectively.


Assuntos
Fumonisinas/metabolismo , Fusarium/classificação , Fusarium/genética , Sorghum/microbiologia , Zea mays/microbiologia , Brasil , Fumonisinas/toxicidade , Fusarium/isolamento & purificação , Fusarium/metabolismo , Genes Fúngicos Tipo Acasalamento , Haplótipos , Repetições de Microssatélites
15.
Braz. j. microbiol ; 36(4): 352-356, Oct.-Dec. 2005. ilus, tab
Artigo em Inglês | LILACS | ID: lil-433473

RESUMO

Um dos fungos mais importantes atualmente em grãos armazenados é o Aspergillus flavus, o qual produz aflatoxinas. Este fungo pode crescer em diversos substratos e representa uma séria preocupação em saúde pública e nutrição animal. Portanto, o estudo de técnicas que possam ser aplicadas no controle das aflatoxinas é de grande importância. Assim sendo, o objetivo do presente trabalho foi estudar os efeitos da radiação gama no crescimento de Aspergillus flavus Link e na degradação das aflatoxinas B1 e B2, (AFB1 e AFB2) em umidade relativa (UR) de 97-99% e atividade de água (Aa) de 0,88-0,94. Amostras de grãos de milho foram irradiadas, utilizando-se uma fonte de Cobalto 60, emissora de raios gama, com as doses de 2; 5 e 10 kGy. A irradiação foi efetiva na redução do número de Unidades Formadoras de Colônias de A. flavus, por grama, nas amostras de milho analisadas. Adicionalmente, o teste de viabilidade fluorescente (solução de diacetato de fluoresceína e brometo de etídio) revelou diminuição no número de células viáveis com o aumento das doses de irradiação e três diferentes padrões de fluorescência. Além disso, a irradiação induziu a uma parcial redução dos níveis de AFB1 e AFB2, nas doses de 2 e 5 kGy, ao passo que uma completa degradação das aflatoxinas foi observada no ensaio empregado com 10 kGy.


Assuntos
Aflatoxinas , Aspergillus flavus , Degradação de Resíduos Químicos , Raios gama , Técnicas In Vitro , Fluorescência , Métodos
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