Children's Utilization of Toys is Moderated by Age-Appropriateness, Toy Category, and Child Age.

Play during childhood is essential to growth and learning. Little is known about whether categories of toys moderate play behaviors at different ages, or how children interact with toys that are simple, appropriate, or complex for their developmental level. Two hundred and forty-three children between the ages of 1 and 8 years, divided into four age groups, played with toys that were targeted to their age group as well as toys aimed at one age group younger and older. Toys fell into nine different categories. Whether children fully utilized each toy was evaluated. Analyses examined how children's utilization of toys was affected by the age-appropriateness of the toy, the category of toy, and the child's age. Considering all age groups and toys, children were less likely to fully utilize toys targeted toward older children than age-appropriate toys, but this effect was moderated by the toy category and the child's age.

Toy Buying Today: Considerations, Information Seeking, and Thoughts about Manufacturer Suggested Age.

Children frequently play with toys purchased by their parents, yet little is known about the how these toys are selected by parents. Mothers with children from 0-12 years of age filled out a survey about their toy purchasing habits. Results revealed that the most common place mothers sought out information about toys was online, and mothers most frequently found the manufacturers' suggested age only somewhat accurate. Further analyses demonstrated that mothers who bought their toys from different sources (i.e., online, stores, secondhand) considered dissimilar items before purchasing a toy for their child and obtained information in distinct ways. In contrast, regardless of where mothers purchased toys, they regarded the manufacturers' suggested age in the same way. This study is one of the few to examine parental toy selection patterns, providing useful knowledge for understanding how to reach parents and provide them information they desire when selecting toys for their children.

ADAPT identifies an ESCRT complex composition that discriminates VCaP from LNCaP prostate cancer cell exosomes.

Libraries of single-stranded oligodeoxynucleotides (ssODNs) can be enriched for sequences that specifically bind molecules on naïve complex biological samples like cells or tissues. Depending on the enrichment strategy, the ssODNs can identify molecules specifically associated with a defined biological condition, for example a pathological phenotype, and thus are potentially useful for biomarker discovery. We performed ADAPT, a variant of SELEX, on exosomes secreted by VCaP prostate cancer cells. A library of ∼1011 ssODNs was enriched for those that bind to VCaP exosomes and discriminate them from exosomes derived from LNCaP prostate cancer cells. Next-generation sequencing (NGS) identified the best discriminating ssODNs, nine of which were resynthesized and their discriminatory ability confirmed by qPCR. Affinity purification with one of the sequences (Sequence 7) combined with LC-MS/MS identified its molecular target complex, whereof most proteins are part of or associated with the multiprotein ESCRT complex participating in exosome biogenesis. Within this complex, YBX1 was identified as the directly-bound target protein. ADAPT thus is able to differentiate exosomes from cancer cell subtypes from the same lineage. The composition of ESCRT complexes in exosomes from VCaP versus LNCaP cells might constitute a discriminatory element between these prostate cancer subtypes.