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J Proteome Res ; 20(2): 1444-1450, 2021 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-33078610

RESUMO

Method development is one of the objectives of the astrophysical community for characterizing the organic matter in objects of the solar system. In this context, we report on the development of an enzyme-catalyzed stereoselective hydrolysis, inspired by the proteomics discipline, which has enabled the indirect detection of peptide sequences in extraterrestrial samples. A proof of concept has been performed on a Murchison extract. We show that our approach can successfully highlight l- and d-amino acids involved in peptide bonds. While we show that some d-amino acids must have been involved in peptide bonds, we cannot at this stage conclude on the indigenous or exogenous nature of these biopolymers. However, our strategy constitutes the first step toward direct UPLC-MS evidence of peptide sequences in extraterrestrial samples. It should thus contribute to deepening knowledge on the molecules available in the solar system, hence providing new clues about their chemical history, especially on Earth.


Assuntos
Meteoroides , Cromatografia Líquida , Meio Ambiente Extraterreno , Peptídeos , Proteômica , Espectrometria de Massas em Tandem
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