RESUMO
In livestock production, lactic acid bacteria (LAB) are the most common microorganisms used as probiotics. For such use, these bacteria must be correctly identified and characterized to ensure their safety and efficiency. In the present study, LAB were isolated from broiler excreta, where a fermentation process was used. Nine among sixteen isolates were identified by biochemical and molecular (sequencing of the 16S rRNA gene) methods as Lactobacillus crispatus (n=1), Lactobacillus pentosus (n=1), Weissella cibaria (n=1), Pediococcus pentosaceus (n=2) and Enterococcus hirae (n=4). Subsequently, these bacteria were characterized for their growth capabilities, lactic acid production, acidic pH and bile salts tolerance, cell surface hydrophobicity, antimicrobial susceptibility and antagonistic activity. Lactobacillus pentosus strain LB-31, which showed the best characteristics, was selected for further analysis. This strain was administered to broilers and showed the ability of modulating the immune response and producing beneficial effects on morpho-physiological, productive and health indicators of the animals.
Assuntos
Criação de Animais Domésticos , Galinhas , Lactobacillales/química , Probióticos/química , Animais , Lactobacillales/genética , Lactobacillales/isolamento & purificação , Lactobacillales/metabolismo , Probióticos/isolamento & purificação , RNA Bacteriano/genética , RNA Bacteriano/metabolismo , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/metabolismo , Análise de Sequência de DNA/veterináriaRESUMO
UNLABELLED: The objective of this study was to isolate and identify yeast strains from broilers excreta and to evaluate in vitro their potential for probiotic use in animal production. METHODS AND RESULTS: Nine yeast strains were isolated and presumptively pre-identified by biochemical assays. These isolates were grouped in six different molecular profiles using PCR-fingerprinting technique. Each profile was identified by sequencing of the D1/D2 domains of the large subunit of the 26S rRNA gene. These yeasts were identified as: Trichosporon sp. (LV-2), Wickerhamomyces anomalus (LV-6), Pichia kudriavzevii (LV-8), Kodamaea ohmeri (LV-9) and Trichosporon asahii (LV-10). A pre-screening of the strains for probiotic use was based on their ability to agglutinate pathogenic micro-organisms. These yeast strains were characterized for specific growth rate, duplication time, their cell surface hydrophobicity, medium acidification, resistance to low pH (2.0, 2.5 and 3.0) and concentrations of bile salts (0.3% and 0.6%). The isolate of W. anomalus (LV-6) had the highest agglutinating and adherence capacity, a growth rate of 2.07×10(8) cfu/mL in 24 h at 30 °C, decreasing the medium pH from 6.5 to 5.23, a 25% hydrophobicity and an elevated capacity to grow under stress conditions. CONCLUSIONS: W. anomalus strain LV-6 showed the best characteristics for use as a probiotic candidate. SIGNIFICANCE AND IMPACT OF THE STUDY: The data from this study helped in choosing a probiotic candidate from yeast to use in broiler production.
Assuntos
Galinhas/imunologia , Probióticos/classificação , Probióticos/isolamento & purificação , Leveduras/classificação , Leveduras/isolamento & purificação , Animais , Bactérias , Impressões Digitais de DNA , DNA Fúngico/genética , Concentração de Íons de Hidrogênio , Leveduras/genética , Leveduras/fisiologiaRESUMO
Se presenta el caso de una mujer de 18 años de edad, que fue intervenida con el diagnóstico presuntivo de un tumor de ovario derecho, evidenciandose durante la intervención quirúrgica la presencia de un tumor pediculado unido al sigmoide, que logró ser resecado totalmente y cuyo reporte Anatomo-patológico fue el de una duplicación intestinal. Se trata de un caso de aparición de dicho cuadro en la edad adulta, la cual se comporto en todo momento como un proceso que simulaba una patología ginecológica. Se estudia el caso y se revisa la literatura