Mecamylamine inhibits seizure-like activity in CA1-CA3 hippocampus through antagonism to nicotinic receptors.

Cholinergic modulation of hippocampal network function is implicated in multiple behavioral and cognitive states. Activation of nicotinic and muscarinic acetylcholine receptors affects neuronal excitability, synaptic transmission and rhythmic oscillations in the hippocampus. In this work, we studied the ability of the cholinergic system to sustain hippocampal epileptiform activity independently from glutamate and GABA transmission. Simultaneous CA3 and CA1 field potential recordings were obtained during the perfusion of hippocampal slices with the aCSF containing AMPA, NMDA and GABA receptor antagonists. Under these conditions, spontaneous epileptiform discharges synchronous between CA3 and CA1 were recorded. Epileptiform discharges were blocked by addition of the calcium-channel blocker Cd2+ and disappeared in CA1 after a surgical cut between CA3 and CA1. Cholinergic antagonist mecamylamine abolished CA3-CA1 synchronous epileptiform discharges, while antagonists of α7 and α4ß2 nAChRs, MLA and DhßE, had no effect. Our results suggest that activation of nicotinic acetylcholine receptors can sustain CA3-CA1 synchronous epileptiform activity independently from AMPA, NMDA and GABA transmission. In addition, mecamylamine, but not α7 and α4ß2 nAChRs antagonists, reduced bicuculline-induced seizure-like activity. The ability of mecamylamine to decrease hippocampal network synchronization might be associated with its therapeutic effects in a wide variety of CNS disorders including addiction, depression and anxiety.

High frequency response of thick REBCO coated conductors in the framework of the FCC study.

A thorough microwave response study of high temperature superconductors, considered as an alternative beam screen coating, has become integral in the design decisions for CERN's future research infrastructure. Here, we present the surface resistance [Formula: see text] of various [Formula: see text] (RE = rare earth) coated conductors available in large scale as a function of magnetic field in a broad temperature range measured by a Hakki-Coleman type resonator with resonant frequency [Formula: see text]. Analysis of the high frequency dissipation supported by DC transport characterization reveals the vortex dynamics in thick [Formula: see text] films. Determined microscopic vortex parameters span over a wide range of magnitudes and reflect the relevance of the superconducting layer's microstructure. We demonstrate that the depinning frequencies [Formula: see text] surpass [Formula: see text], which confirms the operation in high performing, low dissipation pinning regime at measurement conditions. Surface impedance extrapolation to FCC-hh conditions points towards a significant outperformance of copper by coated conductors in terms of surface resistance. The surface resistance margins would open up potential ways for a more efficient frontier circular collider.