Topographical and histological analysis of keratinized mucosal grafts removal techniques an ex-vivo study in porcine mandibles.

The aim of this study was to assess the surface and tissue quality of keratinized mucosa grafts (KMG) obtained using the conventional scalpel and mucotome techniques. This was an experimental in vitro/ex vivo study involving six porcine hemi-mandibles. Specimens were harvested using both the mucotome and conventional scalpel techniques, with randomization determining the choice of technique for tissue removal. The specimens were prepared following predefined laboratory protocols and subsequently subjected to optical microscopy for evaluating epithelial and connective tissue and scanning electron microscopy for topographical and 3D profilometry analysis. Tissues harvested using the mucotome exhibited a linear base and uniform thickness, along with the presence of submucosa and fibrous connective tissue, all of which are ideal for graft success. Differences in the surface characteristics of specimens obtained through the two techniques were observed during a comparative analysis of images obtained through both microscopy types. KMG obtained using the mucotome technique displayed greater uniformity and reduced undesirable cell presence compared to the scalpel technique, thereby enhancing the likelihood of success in soft tissue graft surgical procedures. This study provides valuable insights to oral healthcare professionals and may contribute to future research aimed at achieving more successful surgeries, shorter postoperative recovery times, reduced discomfort, and an overall more positive patient experience.

Clinical and thermographic evaluation after lower third molar extractions and the application of different photobiomodulation protocols: double-blind randomised clinical trial.

OBJECTIVES: to compare the parameters of pain, oedema, temperature, and soft tissue closure in dental sockets that received two different photobiomodulation (PBM) protocols following extractions. MATERIALS AND METHODS: Thirty-one participants had their teeth 38 and 48 extracted. Subsequently, one of the dental sockets received PBM at a wavelength (WL) of 808 nanometers (808 group- nm) and the other dental socket received the PBM at WLs of 808 nm and 660 nm, simultaneously (808 + 660 group). The PBM was applied immediately after the surgical procedure and on the 3rd and 7th days. RESULTS: The mean of Visual Analogue Scale (VAS) values for pain were 1.45 for the 3rd day and 0.52 for the 7th day in the 808 + 660 and 808 group, respectively. The mean the pogonion-tragus measurement, used to assess facial oedema on the 3rd day, was 15.38 cm (range 13.5-17.5) in the 808 + 660 group and 15.48 cm (range 14.0-17.5) in the 808 group. The mean facial temperatures in the 808 + 660 group were 34.9 degrees Celsius (ºC) (range 33.5-36.4) on the 3rd day and 35 ºC (range 33.4-36.4) on the 7th day. In the 808 group, the mean temperatures were 34.9 ºC (range 33.9-36.2) on the 3rd day and 34.9 ºC (33.7-36.2) on the 7th day. Regarding the dimensions of the dental socket, the mean were similar for both groups. Significant differences between the groups were only observed in the pain parameter and only on the 7th day, being greater for the 808 + 660 group (p = 0.031). CONCLUSIONS: The association of the 660 nm with 808 nm, and the increase in energy did not showed more benefits in pain reduction oedema, or acceleration of the closure of the soft tissues of the dental sockets of lower third molars, in the protocols used here. CLINICAL RELEVANCE: There is no need to combine lasers at wavelengths of 660 and 808 nm to reduce oedema, pain and repair of soft tissues after extraction of lower third molars. CLINICAL TRIAL REGISTRATION: This trial was registered in the Brazilian Registry of Clinical Trials (ReBEC) with the following code: RBR-66pyrh8, on 29th December, 2022.

Oral Squamous Cell Carcinoma Lipid Evaluation in Gingiva Tissue Stored in TRIzol via Shotgun Lipidomics and MALDI Mass Spectrometry Imaging.

Oral squamous cell carcinoma (OSCC) is the prevalent type of oral cavity cancer, requiring precise, accurate, and affordable diagnosis to identify the disease in early stages, Comprehending the differences in lipid profiles between healthy and cancerous tissues encompasses great relevance in identifying biomarker candidates and enhancing the odds of successful cancer treatment. Therefore, the present study evaluates the analytical performance of simultaneous mRNA and lipid extraction in gingiva tissue from healthy patients and patients diagnosed with OSCC preserved in TRIzol reagent. The data was analyzed by partial least-squares discriminant analysis (PLS-DA) and confirmed via matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). The lipid extraction in TRIzol solution was linear in a range from 330 to 2000 ng mL-1, r2 > 0.99, intra and interday precision and accuracy <15%, and absolute recovery values ranging from 90 to 110%. The most important lipids for tumor classification were evaluated by MALDI-MSI, revealing that the lipids responsible for distinguishing the OSCC group are more prevalent in the cancerous tissue in contrast to the healthy group. The results exhibit the possibilities to do transcriptomic and lipidomic analyses in the same sample and point out important candidates related to the presence of OSCC.

Accuracy of cone-beam computed tomography in determining gingival thickness: a systematic review and meta-analysis.

OBJECTIVE: To assess the accuracy of cone-beam computed tomography (CBCT) for determining gingival thickness. MATERIAL AND METHODS: Searches were undertaken in PubMed/MEDLINE, EMBASE, LILACS, Web of Science, Scopus, LIVIVO, and gray literature (Google Scholar and ProQuest) for studies considered eligible according to the following criteria: cross-sectional observational studies, which compared CBCT accuracy with that of transgingival probing when determining gingival thickness, in adult patients with good periodontal health. No language or time restrictions were applied in this systematic review. The risk of bias was assessed using the Joanna Briggs Institute (JBI) Critical Appraisal Checklist for analytical cross-sectional studies. RESULTS: Six articles were included for qualitative synthesis, involving a pooled sample of 132 patients with a mean age of 29 years (18-51 years). Of these 6 studies, 5 were eligible for quantitative analysis. The meta-analysis showed no statistically significant difference between CBCT and transgingival probing measures of gingival tissue (mean difference of 0.10 (95% CI-0.17-0.38). No significant level of heterogeneity was detected (Tau2-P = 0.0662; I2 = 0%; H2-P = 1.000; Q-P = 1.134). According to the GRADE criterion, confidence in the cumulative evidence was considered low. CONCLUSIONS: CBCT is an accurate method for determining gingival tissue thickness, comparable to the reference standard (transgingival probing). CLINICAL RELEVANCE: CBCT could be considered for gingival thickness measurement when bone thickness is also needed, and thereby aid in the assessment of gingival biotype without the discomfort and anesthesia needed in transgingival probing. TRIAL REGISTRATION: This protocol was registered at the PROSPERO database (International Prospective Register of Systematic Review) under registration number CRD42022326970. Available from: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022326970 .

Infrared Thermography in the Evaluation of Dental Socket Healing After Photobiomodulation Therapy: A Case Report.

Introduction: The aim of this article was to evaluate the efficacy of photobiomodulation therapy (PBMT) in the alveolar tissue healing process post-extraction using infrared thermography (IT). Case Presentation: A 36-year-old male patient had teeth extractions (18 and 28). Four PBMT sessions (660 nm; 2 J per tooth) were performed in the region of tooth 28 and recorded with thermographic images to compare the healing process, bilaterally. In the first two postoperative sessions, the temperature was higher (hyperradiant) on the left side (treated). After the third laser application, the left side was hyporradiant. In later session, the treated side became hyperradiant compared to the control side. The alveolus of tooth 28 showed more rapid healing than tooth 18 over a period of 60 days. Conclusion: IT can be used to detect the favorable effect of PBMT on accelerating the healing process in the alveolus within 60 days after the tooth extraction.

Local effect of simvastatin combined with different osteoconductive biomaterials and collagen sponge on new bone formation in critical defects in rat calvaria.

PURPOSE: To evaluate the local effect of simvastatin (SVT) combined with deproteinized bovine bone (DBB) with hydroxyapatite/ß-tricalcium phosphate biphasic ceramics (HA/TCP) and with collagen sponge (CS) on bone repair in critical size defects (CSDs) in rat calvaria. METHODS: Forty-two 5-mm diameter CSDs were made bilaterally in the calvaria of 18 rats. The animals were allocated according to the type of biomaterial and associations used to fill the CSD. After 8 weeks, the animals were euthanized, and their calvaria were evaluated for repaired tissue composition using histologic and histometric analyses. RESULTS: In the histometric analysis, the use of SVT showed to increase bone formation in the CSDs when combined with all the bone substitutes tested in this study (p<0.05). Greater bone formation was observed in the groups with SVT compared to the groups without SVT. CONCLUSIONS: The use of SVT without the need for a vehicle and combined with a commercially available biomaterial may be a cheaper way to potentiate the formation of bone tissue without the need to produce new biomaterials. Therefore, SVT combined with DBB induced significantly greater new bone formation than did the other treatments.

Local effect of simvastatin combined with different osteoconductive biomaterials and collagen sponge on new bone formation in critical defects in rat calvaria

Abstract Purpose To evaluate the local effect of simvastatin (SVT) combined with deproteinized bovine bone (DBB) with hydroxyapatite/β-tricalcium phosphate biphasic ceramics (HA/TCP) and with collagen sponge (CS) on bone repair in critical size defects (CSDs) in rat calvaria. Methods Forty-two 5-mm diameter CSDs were made bilaterally in the calvaria of 18 rats. The animals were allocated according to the type of biomaterial and associations used to fill the CSD. After 8 weeks, the animals were euthanized, and their calvaria were evaluated for repaired tissue composition using histologic and histometric analyses. Results In the histometric analysis, the use of SVT showed to increase bone formation in the CSDs when combined with all the bone substitutes tested in this study (p<0.05). Greater bone formation was observed in the groups with SVT compared to the groups without SVT. Conclusions The use of SVT without the need for a vehicle and combined with a commercially available biomaterial may be a cheaper way to potentiate the formation of bone tissue without the need to produce new biomaterials. Therefore, SVT combined with DBB induced significantly greater new bone formation than did the other treatments.

Avaliação do perfil periodontal dos pacientes atendidos na Faculdade de Odontologia da Unievangélica / Periodontal assessment profile of patients treated in the College of Dentistry UniEVANGÉLICA / Periodontal assessment profile of patients treated in the College of Dentistry UniEVANGÉLICA

INTRODUÇÃO: A Doença Periodontal é caracterizada por uma doença infecciosa resultante da inflamação dos tecidos de suporte e do tecido de revestimento dos dentes. Esta pode ser classificada em: gengivite quando compromete o periodonto de proteção e periodontite que acomete os tecidos de inserção. OBJETIVO: Este estudo teve como objetivo verificar o perfil periodontal dos pacientes atendidos na Faculdade de Odontologia da UniEVANGÉLICA, Anápolis- GO. MATERIAL E MÉTODO: Esta pesquisa documental descritiva retrospectiva avaliou prontuários de pacientes atendidos na clínica da Faculdade de Odontologia da UniEVANGÉLICA no período de 2009 a 2014. Nos prontuários foram analisados principalmente o Registro Periodontal Simplificado (PSR), a classificação da doença periodontal e o periograma dos pacientes que apresentaram periodontite. RESULTADOS: Dentre os 233 prontuários, 68% (n = 158) eram mulheres, 33,05% (n = 77) tinham entre 40 e 50 anos e 21% (n = 51) eram fumantes. Destes, 55 % (n = 129) possuíam gengivite e 45% (n=104) periodontite. Dos pacientes com periodontite, a Crônica Localizada foi constatada em 48% (n = 50), a Crônica Generalizada em 40% (n = 42), enquanto as Agressivas Localizada e Generalizada foram encontradas em 7% (n = 7) e 5% (n = 5) respectivamente. Pelos dados do PSR, pode-se observar que 49% (n = 114) apresentaram o código asterisco. CONCLUSÃO: De acordo com os resultados obtidos nesta pesquisa, pode-se notar uma prevalência de pacientes do gênero feminino e a gengivite foi a doença periodontal mais prevalente seguida pela periodontite crônica localizada.

INTRODUCTION: The Periodontal Disease is characterized by an infectious disease resulted from the inflammation of covering tissues (gingivitis) and/or of the support tissue (periodontitis) of teeth. OBJECTIVE: The objective of this study was to verify the periodontal profile of the patients attended at UniEVANGÉLICA’S Dental School. MATERIAL AND METHOD: This is a descriptive documental and retrospective research that evaluated the records of the patients that were attended at UniEvangélica’s Dental School clinic from 2009 to 2014. The Periodontal Screening and Record (PSR), the periodontal disease’s classification and the periodontal chart of the patients that presented periodontitis were the main analysis performed from the records. RESULTS: Among the 233 records, 68% (n=158) were women, 33,05% (n=77) among 40 to 50 years old and 21% (n=51) were smokers. Among these, 55% (n=129) presented gingivitis, from the patients with periodontitis, 48% (n=50) were diagnosed with the Localized Chronic periodontitis, 40% (n=42) with the Generalized Chronic, while 7% (n=7) and 5% (n=5) had the Aggressive Localized and the Generalized Periodontitis, respectively. Through the PSR data, it was noticed that 49% (n=114) presented the asterisk code. CONCLUSION: According to the results of this research, a female prevalence could be noticed and the gingivitis was the most prevalent periodontal disease, followed by the Localized Chronic Periodontitis

Tratamento interdisciplinar na resolução de perda dental anterior precoce: relato de caso clínico / Interdisciplinary treatment in the resolution of anterior missing tooth: a case report

Este relato de caso clínico demonstra a importância da interdisciplinaridade na prática clínica da Odontologia. Para tanto, foi descrito o caso de uma paciente que sofreu um traumatismo bucal, levando a fratura longitudinal no terço coronal radicular do incisivo central superior direito aos seis anos de idade. Foram descritos os procedimentos de tratamento endodôntico, exodontia do dente atingido, tratamento ortodôntico, colocação de enxerto ósseo autógeno no local, implante osseointegrável e, por fim, a colocação de prótese sobreimplante. O acompanhamento, por um longo período, deste caso clínico permitiu a verificação de que o maior sucesso na resolução de casos complexos é alcançado quando existe boa integração de uma equipe interdisciplinar em Odontologia.

The aim of this case report was to demonstrate the importance of the interdisciplinary in Dentistry clinical practice. It was described an oral trauma leading to a longitudinal root fracture on the right upper central incisor in a six years old patient. The procedures endodontics treatment, tooth extraction, orthodontics treatment, the fixation of autogenous bone graft and an implant, and then the installation of the prosthesis were performed. The patient was accompaniment for a long period (1 7 years) and the treatment showed that success in the resolution o/ complex cases is reached when a dentistry team works together.