An ultrasensitive platform for PCB77 detection: New strategy for liquid crystal-based aptasensor fabrication.

Polychlorinated biphenyls (PCBs) are considered persistent bio-accumulative toxicants which threats global food safety and environmental health. Traditional analytical techniques for detection of PCBs are time-consuming and they do not satisfy urgent need for rapid and accurate monitoring of these persistent pollutants. Biosensor technology may be promising in this respect. Here we demonstrate a novel liquid crystal (LC)-based aptasensing platform as a promising label-free and rapid biosensor for PCB77 detection. This novel molecular strategy utilize triple-helix molecular conformational switch which is mediated formation of duplex on sensing platform in presence of target. Duplex forming leads to optical change from dark to bright in a liquid crystal based aptasensor. The limit of quantification of the LC-aptasensor to PCB77 is 1.5 × 10-5 µg/L with comparable selectivity. Besides, we also demonstrated that this system is able to detect PCB77 in tap water, environmental water and milk. This strategy has potential for label-free and portable detection of different targets without any aptamer sequence length restrictions.

Design of a liquid crystal-based aptasensing platform for ultrasensitive detection of tetracycline.

We develop a novel label-free liquid crystal (LC) aptasensor based on intrinsic properties of nematic LCs for ultra-sensitive detection of tetracycline. The aptasensor is assembled by immobilizing aptamers onto the glass slide modified with both homeotropic alignment and silane coupling agents. Designed aptasensor makes use of the target-induced aptamer conformational switching and disruption of the orientation of LCs which lead to an obvious change of the optical appearance from a dark to a bright response. We describe the optimized condition for maintaining the homeotropic orientation of LCs, which are suitable for the tetracycline detection. The average gray-scale intensities of polarizing optical microscopy images were calculated to quantitatively detect tetracycline concentrations. The aptasensor works especially at trace level of tetracycline as low as 0.5 pM. Moreover, the LC aptasensor was successfully used to detect tetracycline in the real milk sample. According to the results, the proposed LC aptasensor for tetracycline detection is simple, ultra-sensitive, label free and ease of preparation.

Recent progress in the development of recognition bioelements for polychlorinated biphenyls detection: Antibodies and aptamers.

Polychlorinated biphenyls (PCBs) are persistent pollutants, which have expanded in foods and the environment. Detection of PCBs is considered essential due to recognized side-effects of PCBs on health and the public concerns in this regard. On the other hand, due to the trace levels of these organic chlorine compounds, reliable and sensitive assays must be developed. Recognition elements are essential parts of analytical detection assays and sensors of PCBs since these elements are involved in the selective identification of the analytes of interest. Understanding the fundamentals of the recognition elements of PCBs and the benefits of the sensor strategies result in the development of next-generation recognition devices. This review aimed to highlight the recent progress in the recognition elements as key parts of biosensors. We initially, focused on the developed antibody-based biosensors for the detection of PCBs, followed by discussing the aptamers as novel recognition elements. Furthermore, the recent advancement in the development of aptamer-based solid phase extractions has been evaluated. These findings could contribute to improving the design of commercial PCB-kits in the future.

Aptasensors as the future of antibiotics test kits-a case study of the aptamer application in the chloramphenicol detection.

Antibiotics are a type of antimicrobial drug with the ubiquitous presence in foodstuff that effectively applied to treat the diseases and promote the animal growth worldwide. Chloramphenicol as one of the antibiotics with the broad action spectrum against Gram-positive and Gram-negative bacteria is widely applied for the effective treatment of infectious diseases in humans and animals. Unfortunately, the serious side effects of chloramphenicol, such as aplastic anemia, kidney damage, nausea, and diarrhea restrict its application in foodstuff and biomedical fields. Development of the sufficiently sensitive methods to detect chloramphenicol residues in food and clinical diagnosis seems to be an essential demand. Biosensors have been introduced as the promising tools to overcome the requirement. As one of the newest types of the biosensors, aptamer-based biosensors (aptasensors) are the efficient sensing platforms for the chloramphenicol monitoring. In the present review, we summarize the recent achievements of the accessible aptasensors for qualitative detection and quantitative determination of chloramphenicol as a candidate of the antibiotics. The present chloramphenicol aptasensors can be classified in two main optical and electrochemical categories. Also, the other formats of the aptasensing assays like the high performance liquid chromatography (HPLC) and microchip electrophoresis (MCE) have been reviewed. The enormous interest in utilizing the diverse nanomaterials is also highlighted in the fabrication of the chloramphenicol aptasensors. Finally, some results are presented based on the advantages and disadvantages of the studied aptasensors to achieve a promising perspective for designing the novel antibiotics test kits.

Self-Assembly of a Structurally Defined Chiro-Optical Peptide-Oligothiophene Hybrid Material.

Conducting polymers are routinely used in optoelectronic biomaterials, but large polymer polydispersity and poor aqueous compatibility complicate integration with biomolecular templates and development of discrete and defined supramolecular complexes. Herein, we report on a chiro-optical hybrid material generated by the self-assembly of an anionic peptide and a chemically defined cationic pentameric thiophene in aqueous environment. The peptide acts as a stereochemical template for the thiophene and adopts an α-helical conformation upon association, inducing optical activity in the thiophene π-π* transition region. Theoretical calculations confirm the experimentally observed induced structural changes and indicate the importance of electrostatic interactions in the complex. The association process is also probed at the substrate-solvent interface using peptide-functionalized gold nanoparticles, indicating that the peptide can also act as a scaffold when immobilized, resulting in structurally well-defined supramolecular complexes. The hybrid complex could rapidly be assembled, and the kinetics of the formation could be monitored by utilizing the local surface plasmon resonance originating from the gold nanoparticles. We foresee that these findings will aid in designing novel hybrid materials and provide a possible route for the development of functional optoelectronic interfaces for both biomaterials and energy harvesting applications.