Toxic Metal Element Concentration in 31 Food Fishes from River Ganga: Risk Assessment on Human Consumption.

Consumption of toxic metal contaminated fish is a significant risk to human health. The Ganga river is one of the vital river systems in India, and it nurtures a rich biodiversity of flora and fauna. In the present study, screening of potential toxic metal elements (Cd, Cr, Mn, Cu, Pb, Ni, Zn, and As) was undertaken in 31 food fishes, especially the small indigenous fishes (SIFs) from the lower stretch of river Ganga by inductively coupled plasma mass spectrometry (ICP-MS). The concentration of toxic metal elements varied among different fish species. Among the toxic metal elements studied, Cr, Zn, and As were found to be dominant in Eleotris fusca; Cd, Ni, and Pb were highest in Securicula gora; Cu was highest in Cabdio morar; and Mn was highest in Coilia dussumieri. The average pollution load index values (APLI) for all the fishes analyzed were less than one except for Eleotris fusca, which indicated no serious toxic metal element pollution load. The estimated daily intake values (EDI) for the toxic metal elements were found to be within the permissible maximum tolerable daily intake (MTDI). Among the fishes studied, only a few species showed hazard index (HI) greater than one, indicating non-carcinogenic health risks. Similarly, the target carcinogenic values (TCR) for most of the toxic metal elements were below the permissible limit (10-4) in the fishes that assures minimal cancer risk. This study provides a comprehensive data on the composition of potential toxic metal elements of 31 food fishes from the lower stretch of the river Ganga, the first of its kind, and suggests the necessity of periodic monitoring of these in the aquatic ecosystem.

Prevalence of Extended-Spectrum ß-Lactamases (ESBLs) Producing Aeromonas spp. Isolated from Lamellidens marginalis (Lamark, 1819) of Sewage-Fed Wetland: A Phenotypic and Genotypic Approach.

The global rise of zoonotic bacteria resistant to multiple antimicrobial classes and the growing occurrence of infections caused by Aeromonas spp. resistant to ß-lactam antibiotics pose a severe threat to animal and human health. However, the contribution of natural environments, particularly aquatic ecosystems, as ideal settings for the development and spread of antimicrobial resistance (AMR) is a key concern. Investigating the phenotypic antibiotic resistance and detection of ß-lactamase producing Aeromonas spp. in Lamellidens marginalis, which inhabit all freshwater ecosystems of the Indian subcontinent, is essential for implications in monitoring food safety and drug resistance. In the present investigation, 92 isolates of Aeromonas spp. were recovered from 105 bivalves and screened for their antimicrobial resistance patterns. In vitro antibiotic resistance profiling showed a higher Multiple Antibiotic Resistance (MAR) index of 0.8 with the highest resistance against ampicillin/sulbactam (82%), while 58, 44, 39 and 38% of the isolates were resistant to cephalothin, erythromycin, cefoxitin and imipenem, respectively. PCR results revealed that these isolates carried the blaTEM gene (94%), which was followed by the blaCTX-M gene (51%) and the blaSHV gene (45%). A combination of blaSHV, blaCTX-M, and blaTEM genes was found in 17% of the isolates, indicating the presence of all three resistance genes. This is the first investigation which highlights the importance of multidrug-resistant Aeromonas spp. in L. marginalis. The identification of extended-spectrum-ß-lactamases (ESBLs) genes demand the necessity of continuous surveillance and systematic monitoring, considering its potential health risks for both animals and human beings.

Hit-to-lead optimization of 2-aminoquinazolines as anti-microbial agents against Leishmania donovani.

Visceral leishmaniasis is a potentially fatal disease caused by infection by the intracellular protist pathogens Leishmania donovani or Leishmania infantum. Present therapies are ineffective because of high costs, variable efficacy against different species, the requirement for hospitalization, toxicity and drug resistance. Detailed analysis of previously published hit molecules suggested a crucial role of 'guanidine' linkage for their efficacy against L. donovani. Here we report the design of 2-aminoquinazoline heterocycle as a basic pharmacophore-bearing guanidine linkage. The introduction of various groups and functionality at different positions of the quinazoline scaffold results in enhanced antiparasitic potency with modest host cell cytotoxicity using a physiologically relevant THP-1 transformed macrophage infection model. In terms of the ADME profile, the C7 position of quinazoline was identified as a guiding tool for designing better molecules. The good ADME profile of the compounds suggests that they merit further consideration as lead compounds for treating visceral leishmaniasis.

Environmental bisphenol A disrupts methylation of steroidogenic genes in the ovary of Paradise threadfin Polynemus paradiseus via abnormal DNA methylation: Implications for human exposure and health risk assessment.

Bisphenol A, endocrine-disrupting chemicals (EDCs) impacting disease development via epigenetic modifications, is crucial in transcriptional regulation. However, ecotoxicology's limited exploration of epigenetics prompted our study's objective: examining the extended exposure of riverine Bisphenol A (BPA), a potent EDC, on DNA methylation during female paradise threadfin (Polynemus paradiseus) reproductive maturation. Assessing BPA contamination in riverine water, we collected fish samples from two locations with distinct contamination levels. In the highly contaminated region (Hc), we observed elevated DNA methylation in aromatase (7.5-fold), 20ß-HSD (3-fold), and FSHR (2-fold) genes. Hormone receptor investigation highlighted an escalating connection between transcriptional hyper-methylation and contamination levels. Additionally, our study revealed a positive correlation between oocyte growth and global DNA methylation, suggesting BPA's potential to modify DNA methylation in female paradise threadfins. This effect likely occurs through changes in hormone receptor expression, persisting throughout oocyte maturation. Notably, our research, the first of its kind in estuarine areas, confirmed BPA contamination in paradise threadfins, raising concerns about potential health risks for humans.

Diel variation of plankton in the highly impacted freshwater zone of Hooghly estuary in relation to ecological alteration.

Plankton are promising ecological monitoring tool that responds quickly to any sort of aquatic ecological alteration, of which many of them are much susceptible to ecological variations. Therefore, monitoring shifts in plankton composition can indicate changes in water quality and aid to identify potential pollution sources. In the present study, the variation in plankton dynamics in relation to ecological variables were monitored in the freshwater zone of the Hooghly estuary from May 2020 to April 2021. The study was conducted in the interval of every six hours. i.e., at 6 A.M., 12 P.M., 6 P.M., and 12 A.M. The present finding revealed the occurrence of 54 phytoplankton and 20 zooplankton taxa/species. Diel variation revealed that among different time intervals, the highest abundance of phytoplankton was recorded 28,307 cells l-1 at 12 P.M, while the lowest was recorded 10,632 cells l-1 at 6 A.M. However, the highest zooplankton abundance was observed 804 ind l-1 at 6 A.M., and the lowest was recorded 156 ind l-1 at 6 P.M. The ANOVA (p < 0.05) analysis indicated significant diel variation for many planktonic genera. The CCA exhibited that most of the phytoplankton were influenced by multiple water quality variables such as temperature, turbidity, calcium, pH, salinity, DO, and nutrients. However, the majority of the zooplankton were affected by turbidity, total phosphorus, sulphate, calcium and available nitrogen. Significant seasonal variation in plankton composition has also been observed. The present study will help to determine the varying diel pattern of planktons in retort to alterations in the water quality parameters and varying ecological niches.

Receptor model-based source apportionment and ecological risk assessment of metals in sediment of river Ganga, India.

Ganga river surface sediment was sampled from 11 locations, which revealed average concentrations (mg/kg) of metals in the order Mn (296.93) > Zn (61.94) > Cr (54.82) > Cu (30.19) > Pb (24.42) > Cd (0.36). Sediment quality guidelines showed metals rarely to occasionally exhibit adverse biological effects. Indices like potential ecological risk, contamination security index, hazard quotients, multiple probable effect concentrations quality, mean probable effects level quotients, mean effects range median quotient suggest nil to a very low level of pollution with low ecological risk. Contamination factor, geo accumulation index, enrichment factor, quantification of contamination revealed that Pb and Cd originated from anthropogenic activities. APCS-MLR model revealed that metals contributed from natural sources (Zn, Mn, Cr; 20.18 %), industrial-agricultural (Cd; 21.35 %); and discharge of paints, Pb batteries, fossil fuel (Pb; 8.49 %). Present findings will serve as an effective guideline for managing and ameliorating pollution in the river system.

Bioaccumulation of polystyrene microplastics and changes in antioxidant and AChE pattern in a freshwater snail (Filopaludina bengalensis) from river Ganga.

Microplastic pollution is a leading global problem affecting terrestrial and marine aquatic ecosystems. Due to the stagnant nature of microplastics, the toxic effect of microplastics is more pronounced to benthic organisms than the surface feeder. Hence, the present study effort was to study the microplastic bioaccumulation pattern and changes in the enzymatic and nonenzymatic antioxidant and AChE pattern of freshwater snail Filopaludina bengalensis, which were subjected to 0.5 ppm to 5 ppm levels of polystyrene microsphere (∼ 30 µm) for 27 days. The study showed that microplastics were easily accumulated in the test organism in a dose and time-dependent manner, amounting to 82 ± 6.02 particles /individuals at a 5 ppm dose on the 27th day. However, no mortality was observed at the test microplastic dosages. The enzymatic antioxidant profile (SOD and catalase) showed limited variability and remained stable with increased duration and microplastic dose. However, the nonenzymatic antioxidant profile showed distinct variability with the complete seizing of the DPPH activity on the 27th day at 5 ppm microplastic dose and a gradual decrease of ABTS and FRAP activity at all the dose ranges. Even the AChE activity decreased with higher exposure concentrations. The present study for the first time shows the direct impact of microplastics on a freshwater snail widely available in the Indian subcontinent, indicating the role of microplastic pollution will create havoc in the Ganga river eco-biosystem in the long run.

Hemato-biochemical alteration in the bronze featherback Notopterus notopterus (Pallas, 1769) as a biomonitoring tool to assess riverine pollution and ecology: a case study from the middle and lower stretch of river Ganga.

Fishes are poikilothermic animals and are rapid responders to any sort of ecological alteration. The responses in the fish can be easily assessed from their hematological and biochemical responses. To study the variation in the hemato-biochemical parameters in retort to ecological alteration and ecological regime, a study was conducted at six different sampling stations of the middle and lower stretches of river Ganga. Various hematological and biochemical responses of fishes were also monitored in response to multiple ecological alterations. For the assessment of ecological alteration, various indices were calculated such as the water pollution index (WPI), National Sanitation Foundation-water quality index (NSF-WQI), and Nemerow's pollution index (NPI) has been calculated based on various water quality parameters such as dissolved oxygen (DO), pH, total dissolved solids (TDS), total alkalinity (TA), total hardness (TH), electrical conductivity (EC), biochemical oxygen demand (BOD), chlorinity (CL), total nitrogen (TN), and total phosphorus (TP). The hematological parameters such as WBC, RBC, platelet, hemoglobin, and hematocrit were monitored. The serum biochemical parameters such as SGPT, SGOT, ALP, amylase, bilirubin, glucose, triglyceride (TRIG), and cholesterol (CHOL) were investigated. The study revealed that NSF-WQI varied from 45.08 at Buxar to 110.63 at Rejinagar and showed a significantly positive correlation with SGPT, SGOT, ALP, TRIG, CHOL, and WBC, whereas a significantly negative correlation was observed between TRIG and RBC. WPI varied from 19 to 23 and showed a significant positive correlation with SGOT and a negative correlation was observed with total nitrogen. The PCA analysis illustrated the significance of both natural as well as anthropogenic factors on riverine ecology. Strong positive loading was observed with SGPT, SGOT, ALP, and platelet. The study signified the need for monitoring the hemato-biochemical responses of fishes in response to alterations in the ecological regime.

Encoding the Photoreceptors of the Human Eye.

This review aims to assess the anatomy of the human eye with a focus on exploring opportunities to mimic certain functionalities of photoreceptors in the optical system. This can help restore vision issues in people who had normal vision earlier, but their vision was impaired due to reasons that damaged parts of the eye; however, the functionality of the optic nerve remained intact. It is a conceptual article where the methodology to simulate artificial photoreceptors is discussed.