Assuntos
Arenavirus do Novo Mundo/isolamento & purificação , Arenavirus do Velho Mundo/isolamento & purificação , Reservatórios de Doenças , Muridae/virologia , Doenças dos Roedores/virologia , Animais , Infecções por Arenaviridae/transmissão , Infecções por Arenaviridae/veterinária , Infecções por Arenaviridae/virologia , Arenavirus do Novo Mundo/crescimento & desenvolvimento , Arenavirus do Velho Mundo/crescimento & desenvolvimento , Humanos , Doenças dos Roedores/transmissãoRESUMO
Hantavirus pulmonary syndrome (HPS), a severe respiratory disease with high mortality caused by rodent-borne hantaviruses, has previously been identified in the United States and Canada as well as central and southern South America. In late 1999 and early 2000, an outbreak of acute illness compatible with HPS was reported in Los Santos, Panama, with the death of 3 of the 12 (25%) suspected cases. Hantavirus-specific antibodies were detected in patient sera, and virus RNA was detected by reverse transcriptase-polymerase chain reaction. Sequence analysis of virus genome N-, G1-, and G2-encoding fragments showed this to be a novel hantavirus, Choclo virus. Serologic and virus genetic analyses of rodents trapped in the area showed Oligoryzomys fulvescens to be the likely reservoir for the HPS-associated Choclo virus. In addition, Zygodontomys brevicauda rodents were shown to harbor another genetically unique hantavirus, Calabazo virus.
Assuntos
Reservatórios de Doenças , Síndrome Pulmonar por Hantavirus/virologia , Orthohantavírus/classificação , Filogenia , Animais , Canadá , Primers do DNA , Genoma Viral , Orthohantavírus/genética , Orthohantavírus/isolamento & purificação , Síndrome Pulmonar por Hantavirus/classificação , Humanos , Nucleocapsídeo/genética , Panamá , Ratos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sorotipagem , América do Sul , Estados UnidosRESUMO
The flow cytometry methods (FCM) was employed to determine cellular DNA content of black-crowned night-heron (Nycticorax nycticorax) embryos and 10-day-old chicks collected at sites differing in types of chemical contamination. The coefficient of variation of DNA content (CV) in blood collected from embryos suggested cytogenetic damage at a side in Louisiana known to be contaminated with petroleum. Blood CV from chicks suggested genetic damage at a site in Texas also known to be contaminated with petroleum. Spleen CVs in chicks were significantly lower than respective means from the reference site. The CVs of chick blood and liver and spleen negatively correlated, suggesting recovery of spleen and liver cells after exposure to a clastogenic compound. Thus, the lower CVs may also have been indicative of genetic damage. Based on the findings of this study, FCM is a potential indicator of certain environmental contaminants in black-crowned night-herons.
Assuntos
Aves/genética , DNA/efeitos dos fármacos , Monitoramento Ambiental/métodos , Poluentes Ambientais/toxicidade , Citometria de Fluxo , Animais , DNA/análise , DNA/sangue , Fígado/química , Baço/químicaRESUMO
Genome size (the amount of DNA per cell) was measured by flow-cytometric analysis in seven species of a chromosomally variable rodent genus: Ctenomys boliviensis, C. conoveri, C. frater, C. leucodon, C. lewisi, C. opimus, and C. steinbachi. The mean genome size of these species was 7.19 pg DNA and little inter- and intra-specific variation was observed. Genome size was not correlated with diploid number, suggesting that chromosomal evolution at this level is independent of total DNA content. A hypothetical taxonomic unit optimization procedure was carried out using genome size change on a Wagner tree derived from allozyme data. Allozyme evolution and genome size change are linked by a weak, but significant, negative correlation suggestive of preferential genic evolution in the absence of genome size evolution.