RESUMO
Anthracnose fruit rot (AFR), caused by the fungal pathogen Colletotrichum fioriniae, is among the most destructive and widespread fruit disease of blueberry, impacting both yield and overall fruit quality. Blueberry cultivars have highly variable resistance against AFR. To date, this pathogen is largely controlled by applying various fungicides; thus, a more cost-effective and environmentally conscious solution for AFR is needed. Here we report three quantitative trait loci associated with AFR resistance in northern highbush blueberry (Vaccinium corymbosum). Candidate genes within these genomic regions are associated with the biosynthesis of flavonoids (e.g. anthocyanins) and resistance against pathogens. Furthermore, we examined gene expression changes in fruits following inoculation with Colletotrichum in a resistant cultivar, which revealed an enrichment of significantly differentially expressed genes associated with certain specialized metabolic pathways (e.g. flavonol biosynthesis) and pathogen resistance. Using non-targeted metabolite profiling, we identified a flavonol glycoside with properties consistent with a quercetin rhamnoside as a compound exhibiting significant abundance differences among the most resistant and susceptible individuals from the genetic mapping population. Further analysis revealed that this compound exhibits significant abundance differences among the most resistant and susceptible individuals when analyzed as two groups. However, individuals within each group displayed considerable overlapping variation in this compound, suggesting that its abundance may only be partially associated with resistance against C. fioriniae. These findings should serve as a powerful resource that will enable breeding programs to more easily develop new cultivars with superior resistance to AFR and as the basis of future research studies.
RESUMO
Este estudo analisou a aplicação das práticas discursivas e produção de sentidos na descrição e interpretação dos sentidos dados a diferentes fenômenos em saúde mental no Brasil. Trata-se de uma revisão bibliográfica baseada na literatura especializada, a partir da consulta a estudos científicos selecionados utilizando a Biblioteca Virtual em Saúde (BVS) com as palavras-chave 'práticas discursivas' e 'produção de sentidos', no título, resumo ou assunto, resultando na seleção de 21 estudos, dada sua relação com a saúde mental. A aplicação desta teoria se deu na abordagem profissional e usuário, assistência terapêutica, formação profissional, maternidade, paternidade, adoção e percepção pessoal de fenômenos como crise, vulnerabilidade social, doença, cuidado, self, exclusão social e violência. Compreender esses fenômenos possibilita a reflexão sobre a práxis de profissionais de saúde mental e afins, contribuindo com estratégias de acolhimento e formação de vínculo na relação entre profissional e usuário a partir da interpretação dos sentidos.
This study has analyzed the application of discursive practices and production of meanings in the description and interpretation of the meanings given to different phenomena in mental health in Brazil. This is a bibliographical review based on specialized literature from the inquiry to selected scientific studies employing the Virtual Health Library (VHL) with the following keywords: 'discursive practices' and 'production of meanings', in the title, abstract or subject, resulting in the selection of 21 studies, given their relation to the mental health. The application of this theory is on the professional approach and user, therapeutic assistance, professional training, maternity, paternity, adoption and personal perception of phenomena such as crisis, social vulnerability, illness, care, self, social exclusion and violence. Understanding these phenomena enables the reflection about the praxis of mental health professionals and the like, contributing with strategies of integration and formation of links in the relationship between the professional and the user from the interpretation of the meanings.
Este estudio analizó la aplicación de las prácticas discursivas y producción de los sentidos en la descripción e interpretación de los sentidos dados a diferentes fenómenos en salud mental en Brasil. Se trata de una revisión bibliográfica basada en la literatura especializada, a partir de la consulta a estudios científicos utilizando la Biblioteca Virtual en Salud (BVS) con las palabras clave: 'prácticas discursivas' y 'producción de sentidos', en el título, resumen o asunto, resultando en la selección de 21 estudios, dada su relación con la salud mental. La aplicación de esta teoría se dio en el enfoque profesional y usuario, asistencia terapéutica, formación profesional, maternidad, paternidad, adopción, y percepción personal de fenómenos como crisis, vulnerabilidad social, enfermedad, cuidado, self, exclusión social y violencia. Comprender estos fenómenos posibilita la reflexión sobre la praxis de profesionales de salud mental y afines, contribuyendo con estrategias de acogida y formación de vinculo en la relación entre profesional y usuario a partir de los sentidos.