A computational study on the structure-function relationships of plant caleosins.

Plant cells store energy in oil bodies constructed by structural proteins such as oleosins and caleosins. Although oil bodies usually accumulate in the seed and pollen of plants, caleosins are present in various organs and organelles. This issue, coupled with the diverse activities of caleosins, complicates the description of these oleo-proteins. Therefore, the current article proposes a new classification based on the bioinformatics analysis of the transmembrane topology of caleosins. Accordingly, the non-membrane class are the most abundant and diverse caleosins, especially in lower plants. Comparing the results with other reports suggests a stress response capacity for these caleosins. However, other classes play a more specific role in germination and pollination. A phylogenetic study also revealed two main clades that were significantly different in terms of caleosin type, expression profile, molecular weight, and isoelectric point (P < 0.01). In addition to the biochemical significance of the findings, predicting the structure of caleosins is necessary for constructing oil bodies used in the food and pharmaceutical industries.

Economic purification of recombinant uricase by artificial oil bodies.

Rasburicase is an expensive treatment used to control hyperuricemia caused by tumour lysis syndrome (TLS). In this study, a non-chromatographic method was designed based on nano-oil bodies for convenient and economical purification of the recombinant uricase. For this purpose, two chimaeras were synthesized with a different arrangement of the uricase, caleosin and intein fragments. After confirming the protein expression by measuring the uricase activity at 293 nm, purification was conducted through oil-body construction. The results were resolved on the 12% SDS-PAGE gel. Finally, the stability of the oil bodies was examined against different salts, surfactants, temperatures, and pH values. According to our results, the overexpression of uricase-caleosin chimaera under the T7 promoter in Escherichia coli led to the production of soluble protein, which was successfully purified by artificial oil bodies. The active uricase was subsequently released through the self-splicing of intein. Further investigations highlighted the importance of the free C-terminus of caleosin in constructing artificial oil bodies. Moreover, surfactants and low temperature, in contrast to salts, improved the stability of oil bodies. In conclusion, caleosins are an efficient purification tag reducing the cost of purification compared to conventional chromatography methods.

Frequency Dependence of Shear Wave Velocity in Stroke-Affected Muscles During Isometric Contraction- Preliminary Data.

In addition to changes in the central nervous system, many changes can occur in the composition and structure of skeletal muscles after a hemispheric stroke. The mechanical behavior of skeletal muscles is linked to the density and structural arrangement of key constituents. Yet, little is known about changes in post-stroke muscle mechanical properties such as viscoelasticity. The aim of this study was to explore the frequency-dependent changes in shear wave (SW) velocity as a potentially informative feature accompanying changes in muscle viscoelastic properties under passive and active conditions in hemiplegic stroke. We used the ultrasound SuperSonic Imaging technique to induce and measure SW propagation in the biceps brachii muscle for both the paretic and contralateral limbs in three hemiplegic stroke survivors during passive and submaximal voluntary muscle contractions. We found that for all subjects, the muscles on both the paretic and non-paretic sides demonstrated large dispersion (i.e., a change in SW phase velocities as a function of frequency within each contraction level) under both passive and active conditions, although muscles on the paretic side displayed larger dispersion. In addition, for a range of frequencies from 108-756 Hz, the SW phase velocity was higher in active nonparetic muscles compared to those of paretic side with an increase of 42% at 756 Hz. This is in contrast with the muscle response under passive condition where the SW phase velocity exhibited a 97 % increase at 765Hz on the paretic side compared to the non-paretic side. These results suggest the mechanical properties are altered for stroke-affected muscles, which may be a result of changes in the muscle extracellular matrix composition. Further, this study provides evidence that there are changes in tissue mechanical properties and that may consequently influence muscle function.

A review on chimeric xylanases: methods and conditions.

Multi-functional enzymes are one of the nature's solutions to facilitate metabolic pathways, thus several reactions are regulated and performed simultaneously on one polypeptide chain. Inspired by nature, artificial chimeric proteins have been designed to reduce the production costs and improve the performance. One of the interesting applications of this method is in the plant-based industries such as feed additive, waste treatment, biofuel production, and pulp and paper bleaching. In fact, the heterogeneous texture of plants needs using a combination of different enzymes to achieve an optimal quality in the manufacturing process. Given that xylans are the most abundant non-cellulosic polysaccharides in nature, xylanases are widely utilized in the mentioned industries. In this regard, several studies have been conducted to develop the relevant chimeric enzymes. Despite the successes that have been attained in this field, misfolding, functional or structural interference, and linker breakage have been reported in some cases. The present paper reviews the research to introduce the prerequisites to design an appropriate chimeric xylanase.

The concentration of stress at the rotator cuff tendon-to-bone attachment site is conserved across species.

The tendon-to-bone attachment site integrates two distinct tissues via a gradual transition in composition, mechanical properties, and structure. Outcomes of surgical repair are poor, in part because surgical repair does not recreate the natural attachment, and in part because the mechanical features that are most critical to mechanical and physiological functions have not been identified. We employed allometric analysis to resolve a paradox about how the architecture of the rotator cuff contributes to load transfer: whereas published data suggest that the mean muscle stresses expected at the tendon-to-bone attachment are conserved across species, data also show that the relative dimensions of key anatomical features vary dramatically, suggesting that the amplification of stresses at the interface between tendon and bone should also vary widely. However, a mechanical model that enabled a sensitivity analysis revealed that the degree of stress concentration was in fact highly conserved across species: the factors that most affected stress amplification were most highly conserved across species, while those that had a lower effect showed broad variation across a range of relative insensitivity. Results highlight how micromechanical factors can influence structure-function relationships and cross-species scaling over several orders of magnitude in animal size, and provide guidance on physiological features to emphasize in surgical and tissue engineered repair of the rotator cuff.

Effective elastic properties of a composite containing multiple types of anisotropic ellipsoidal inclusions, with the application to the attachment of tendon to bone.

Estimates of the effective stiffness of a composite containing multiple types of inclusions are needed for the design and study of functionally graded systems in engineering and physiology. While excellent estimates and tight bounds exist for composite systems containing specific classes and distributions of identical inclusions, these are not easily generalized to complex systems with multiple types of inclusions. For example, three-point parameters are known for only a few inclusion shapes and orientations. The best estimate available for a composite containing multiple classes of inclusions arises from the Kanaun-Jeulin approach. However, this method is analogous to a generalized Benveniste approach, and therefore suffers from the same limitations: while excellent for low volume fractions of inclusions, the Kanaun-Jeullin and Benveniste estimates lie outside of three-point bounds at higher volume fractions. Here, we present an estimate for composites containing multiple classes of aligned ellipsoidal inclusions that lies within known three-point bounds at relatively higher volume fractions of inclusions and that is applicable to many engineering and biological composites.

Micromechanical modeling of elastic properties of cortical bone accounting for anisotropy of dense tissue.

The paper analyzes the connection between microstructure of the osteonal cortical bone and its overall elastic properties. The existing models either neglect anisotropy of the dense tissue or simplify cortical bone microstructure (accounting for Haversian canals only). These simplifications (related mostly to insufficient mathematical apparatus) complicate quantitative analysis of the effect of microstructural changes - produced by age, microgravity, or some diseases - on the overall mechanical performance of cortical bone. The present analysis fills this gap; it accounts for anisotropy of the dense tissue and uses realistic model of the porous microstructure. The approach is based on recent results of Sevostianov et al. (2005) and Saadat et al. (2012) on inhomogeneities in a transversely-isotropic material. Bone's microstructure is modeled according to books of Martin and Burr (1989), Currey (2002), and Fung (1993) and includes four main families of pores. The calculated elastic constants for porous cortical bone are in agreement with available experimental data. The influence of each of the pore types on the overall moduli is examined.

Virtual Screening of Anti-Mycobacterial Plant Compounds.

Deaths from tuberculosis have long gripped people and threatened human health. The need for new drug compounds are critically sensed by medical scientists and practitioners due to the emergence of new strains and the slow rate of discovering novel medicines for this disease. Since plants are a rich source of diverse drug compounds, they are among the best choices to achieve new ones. The study of all plants or their compounds is an almost impossible scenario; hence bio/cheminformatics methodology can be used to reduce time and cost spent in drug discovery. For this purpose, we made several databases of anti-mycobacterial plant compounds and further found filter criteria which were able to describe more predicted bioactive compounds by the established algorithm. Also, we present the survey of the developed resource by using bio/cheminformatics tools. The presence of several anti-mycobacterial compounds in the predicted algorithm and introduction of new active compounds represent the high potential of this method. In addition, the general profile of such bioactive molecules is pinpointed using molecular descriptors and cheminformatics approach.