L-Carnitine Combined with Leucine Supplementation Does Not Improve the Effectiveness of Progressive Resistance Training in Healthy Aged Women.

OBJECTIVES: To evaluate the effect of L-carnitine (LC) in combination with leucine supplementation on muscle strength and muscle hypertrophy in aged women participating in a resistance exercise training (RET) program. DESIGN/SETTING/PARTICIPANTS: Thirty-seven out of sixty (38.3% dropout) healthy women aged 60-75 years (mean 67.6 ± 0.7 years) completed the intervention in one of three groups. One of the supplemented groups received 1 g of L-carnitine-L-tartrate in combination with 3 g of L-leucine per day (LC+L group; n = 12), and the second supplemented group received 4 g of L-leucine per day (L group; n = 13). The control group (CON group; n = 12) received no supplementation. INTERVENTION: All three groups completed the same RET protocol involving exercise sessions twice per week for 24 weeks. MEASUREMENTS: Before and after the experiment, participants performed isometric and isokinetic muscle strength testing on the Biodex dynamometer. The cross-sectional areas of the major knee extensors and total thigh muscles were assessed using magnetic resonance imaging. Fasting serum levels of insulin-like growth factor-1 (IGF-1), myostatin and decorin, and plasma levels of total carnitine (TC) and trimethylamine-N-oxide (TMAO) levels were measured. RESULTS: The 24-week RET significantly increased muscle strength and muscle volume, but the group and time interactions were not significant for the muscle variables analyzed. Plasma total carnitine increased only in the LC+L group (p = 0.009). LC supplementation also caused a significant increase in plasma TMAO, which was higher after the intervention in the LC+L group than in the L (p < 0.001), and CON (p = 0.005) groups. The intervention did not change plasma TMAO concentration in the L (p = 0.959) and CON (p = 0.866) groups. After the intervention serum decorin level was higher than before in both supplemented groups combined (p = 0.012), still not significantly different to post intervention CON (p = 0.231). No changes in serum IGF-1 and myostatin concentrations and no links between the changes in blood markers and muscle function or muscle volume were observed. CONCLUSIONS: LC combined with leucine or leucine alone does not appear to improve the effectiveness of RET.

Tractography-guided surgery of brain tumours: what is the best method to outline the corticospinal tract?

BACKGROUND: Diffusion tensor imaging (DTI) is the imaging technique used in vivo to visualise white matter pathways. The cortico-spinal tract (CST) belongs to one of the most often delineated tracts preoperatively, although the optimal DTI method has not been established yet. Considering that various regions of interests (ROIs) could be selected, the reproducibility of CST tracking among different centres is low. We aimed to select the most reliable tractography method for outlining the CST for neurosurgeons. MATERIALS AND METHODS: Our prospective study consisted of 32 patients (11 males, 21 females) with a brain tumour of various locations. DTI and T1-weighed image series were acquired prior to the surgery. To draw the CST, the posterior limb of the internal capsule (PLIC) and the cerebral peduncle (CP) were defined as two main ROIs. Together with these main ROIs, another four cortical endpoints were selected: the frontal lobe (FL), the supplementary motor area (SMA), the precentral gyrus (PCG) and the postcentral gyrus (POCG). Based on these ROIs, we composed ten virtual CSTs in DSI Studio. The fractional anisotropy, the mean diffusivity, the tracts' volume, the length and the number were compared between all the CSTs. The degree of the CST infiltration, tumour size, the patients' sex and age were examined. RESULTS: Significant differences in the number of tracts and their volume were observed when the PLIC or the CP stood as a single ROI comparing with the two- ROI method (all p < 0.05). The mean CST volume was 40054U (SD ± 12874) and the number of fibres was 259.3 (SD ± 87.3) when the PLIC was a single ROI. When the CP was a single ROI, almost a half of fibres (147.6; SD ± 64.0) and half of the CST volume (26664U; SD ± 10059U) was obtained (all p < 0.05). There were no differences between the various CSTs in terms of fractional anisotropy, mean diffusivity, the apparent diffusion coefficient, radial diffusivity and the tract length (p > 0.05). The CST was infiltrated by a growing tumour or oedema in 17 of 32 patients; in these cases, the mean and apparent diffusion of the infiltrated CST was significantly higher than in uncompromised CSTs (p = 0.04). CST infiltration did not alter the other analysed parameters (all p > 0.05). CONCLUSIONS: A universal method of DTI of the CST was not developed. However, we found that the CP or the PLIC (with or without FL as the second ROI) should be used to outline the CST.

Frontal aslant tract projections to the inferior frontal gyrus.

BACKGROUND: Frontal aslant tract (FAT) is a white matter bundle connecting the pre-supplementary motor area (pre-SMA) and the supplementary motor area (SMA) with the inferior frontal gyrus (IFG). The purpose of the present study was to evaluate the anatomical variability of FAT. MATERIALS AND METHODS: Total number of fibres and the lateralisation index (LI) were calculated. We attempted to find factors contributing to the diversity of FAT regarding IFG terminations to the pars opercularis (IFG-Op) and to the pars triangularis (IFG-Tr). Magnetic resonance imaging of adult patients with diffusion tensor imaging (DTI) with total number of 98 hemispheres composed a cohort. V-shaped operculum was the most common (60.5%). RESULTS: Total number of FAT fibres had widespread and unimodal distribution (6 to 1765; median: 160). Left lateralisation was noted in 64.3% of cases and was positively correlated with total number of FAT fibres and the bundle projecting to IFG-Op (p < 0.01). LI correlated with total number of FAT fibres (r = 0.43, p < 0.01). FAT projected predominantly to IFG-Op (88.9%; 88 of 99). Only in 3 (3.1%) cases more fibres terminated in IFG-Tr than in IFG-Op. Total number of FAT fibres and number of fibres terminating at IFG-Op did not correlate with the ratio of fibre numbers: FAT/IFG-Op, FAT/IFG-Tr and IFG-Op/IFG-Tr (p > 0.05). The greater total number of fibres to IFG-Tr was, the higher were the ratios of IFG-Tr/ /FAT (r = 0.57, p < 0.01) and IFG-Tr/IFG-Op (r = 0.32, p = 0.04). CONCLUSIONS: Among the IFG, the major termination of FAT is IFG-Op. Whereas the IFG-Tr projection seems to be related to the expansion of the entire FAT bundle regardless of side, domination and handedness. Nevertheless, FAT features a significant anatomical variability which cannot be explained in terms of DTI findings.

Brain resting state functional magnetic resonance imaging in patients with takotsubo cardiomyopathy an inseparable pair of brain and heart.

BACKGROUND: Takotsubo cardiomyopathy (TTC) is often triggered by emotional or physical stress factors. Psychological variables can have an impact on the physical manifestations of heart disease. TTC may reflect stunned myocardium from a neurogenic source. Anatomical connections between different parts of the brain can be visualized by diffusion tensor imaging (DTI) and thus, expressed by diffusion coefficient - fractional anisotropy (FA). A novel tool used to evaluate brain function in the absence of task is resting state functional magnetic resonance imaging (RS-fMRI). METHODS: The study included both psychological tests and RS-fMRI examination, and was performed uniformly, in patients with takotsubo and healthy controls. The final group of patients consisted of 13 women, each who underwent a typical pattern of TTC triggered by emotionally stressful factors. The control group included thirteen healthy, age-matched women. RESULTS: Psychological tests revealed that the Type D personality was not more likely to appear among studied patients with takotsubo cardiomyopathy than amongst the healthy population. However, the level of anxiety seen in patients with TTC was increased. There were no differences in FA values between patients and healthy controls. RS-fMRI showed that TTC patients had increased connectivity areas in the precuneus. The healthy controls, when compared to TTC patients had increased connectivity in the ventromedial prefrontal cortex. CONCLUSIONS: Taking into account the RS-fMRI results, psychological testing may suggest that TTC patients place a greater focus on themselves (increased tendency to experience negative affectivity, greater conscientiousness) and might have problems with emotional control. Our findings lead to the hypothesis that there is a personality profile for TTC patients' reactions to stressful triggers.