Seroepidemiological study on coinfection of toxoplasmosis and active tuberculosis in Northern Iran: a case control study.

Coinfection of tuberculosis (TB) and human parasitic infections is common in developing countries. There is little information about the prevalence of Toxoplasma gondii (T. gondii) infection among TB patients in Iran. In this case-control study, anti-toxoplasma antibodies were measured by ELISA method in 100 patients with active tuberculosis and 100 healthy individuals who were matched in terms of sex, age, and place of residence. Anti-T. gondii IgG antibodies were diagnosed in 62% of TB patients (95% CI 53-71%) and 70% of control subjects (95% CI 62-78%). Anti-T. gondii IgM antibodies were found in 1% of both TB patients and control group. The seroprevalence of T. gondii infection was not significantly different between TB patients and healthy individuals (P > 0.05). None of the assessed sociodemographic and behavioral factors was recognized as a risk factor for toxoplasmosis in TB infected patients. Moreover, the level of anti-T. gondii IgG antibodies concentration in TB patients was significantly higher than in control subjects and revealed skewness towards humoral immune response in TB patients. Coinfection of toxoplasmosis and tuberculosis was prevalent but T. gondii infection was independent of active TB in this co-endemic area.

Seaweed-derived phenolic compounds as diverse bioactive molecules: A review on identification, application, extraction and purification strategies.

Seaweed, a diverse group of marine macroalgae, has emerged as a rich source of bioactive compounds with numerous health-promoting properties. Among these, phenolic compounds have garnered significant attention for their diverse therapeutic applications. This review examines the methodologies employed in the extraction and purification of phenolic compounds from seaweed, emphasizing their importance in unlocking the full potential of these oceanic treasures. The article provides a comprehensive overview of the structural diversity and biological activities of seaweed-derived phenolics, elucidating their antioxidant, anti-inflammatory, and anticancer properties. Furthermore, it explores the impact of extraction techniques, including conventional methods and modern green technologies, on the yield and quality of phenolic extracts. The purification strategies for isolating specific phenolic compounds are also discussed, shedding light on the challenges and advancements in this field. Additionally, the review highlights the potential applications of seaweed-derived phenolics in various industries, such as pharmaceuticals, cosmetics, and functional foods, underscoring the economic value of these compounds. Finally, future perspectives and research directions are proposed to encourage continued exploration of seaweed phenolics, fostering a deeper understanding of their therapeutic potential and promoting sustainable practices in the extraction and purification processes. This comprehensive review serves as a valuable resource for researchers, industry professionals, and policymakers interested in harnessing the untapped potential of phenolic compounds from seaweed for the betterment of human health and environmental sustainability.

Prenatal exposure to crude oil vapor reduces differentiation potential of rat fetal mesenchymal stem cells by regulating ERK1/2 and PI3K signaling pathways: Protective effect of quercetin.

It has been indicated that crude oil vapor (COV) induces tissue damage by several molecular mechanisms. Quercetin (QT) as an important component of food with antioxidant properties has a protective role against cell toxicity caused by many pollutants. However, data related to the adverse effects of crude oil vapor (COV) on stem cell fate and differentiation and the role of quercetin (QT) in protecting stem cells against the toxicity caused by these pollutants is very limited. This study aimed to explore the protective effect of QT against the adverse effects of COV on fetal mesenchymal stem cells (fMSCs) differentiation. Twenty-four pregnant Wistar rats were categorized into 4 groups including the control, COV, COV+QT, and QT. Rats were exposed to COV from gestational day (GD) 0-15 and received QT by gavage. The fMSCs were isolated from fetuses, and cell proliferation, differentiation potential, expression of osteogenesis and adipogenesis-related genes, and phosphorylation of PI3K and ERK1/2 signaling proteins were evaluated. The results showed that COV reduced the proliferation and differentiation of fMSCs through the activation of PI3K and ERK1/2 signaling pathways. Also, COV significantly decreased the expression of osteonectin, ALP, BMP-6, Runx-2, PPARγ, and CREBBP genes in differentiated cells. QT treatment increased the proliferation and differentiation of fMSCs in COV-exposed rats. In conclusion, our findings suggest that prenatal exposure to COV impaired fMSCs differentiation and QT reduced the adverse effects of COV by regulating ERK1/2 and PI3K signaling pathways.

Sex differences in cardiac rehabilitation barriers among non-enrollees in the context of lower gender equality: a cross-sectional study.

BACKGROUND: Despite the benefits of cardiac rehabilitation (CR), it remains under-utilized, particularly by women. This study compared CR barriers between non-enrolling men and women in Iran, which has among the lowest gender equality globally. METHODS: In this cross-sectional study, CR barriers were assessed via phone interview in phase II non-attenders from March 2017 to February 2018 with the Persian version of the Cardiac Rehabilitation Barriers Scale (CRBS-P). T-tests were used to compare scores, with each of 18 barriers scored out of 5, between men and women. RESULTS: 357 (33.9%) of the sample of 1053 were women, and they were older, less educated and less often employed than men. Total mean CRBS scores were significantly greater in women (2.37 ± 0.37) than men (2.29 ± 0.35; effect size[ES] = 0.08, confidence interval[CI]: 0.03-0.13; p < 0.001). The top CR barriers among women were cost (3.35; ES = 0.40, CI:0.23-0.56; P < 0.001), transportation problems (3.24; ES = 0.41, CI:0.25-0.58; P < 0.001), distance (3.21; ES = 0.31, CI:0.15-0.48; P < 0.001), comorbidities (2.97; ES = 0.49, CI:0.34-0.64; P < 0.001), low energy (2.41; ES = 0.29, CI:0.18-0.41; P < 0.001), finding exercise as tiring or painful (2.22; ES = 0.11, CI:0.02-0.21; P = 0.018), and older age (2.27; ES = 0.18, CI:0.07-0.28; P = 0.001). Men rated "already exercise at home or in community" (2.69; ES = 0.23, CI:0.1-0.36; P = 0.001), time constraints (2.18; ES = 0.15, CI:0.07-0.23; P < 0.001) and work responsibilities (2.24; ES = 0.16, CI:0.07-0.25; P = 0.001) as greater CR barriers than women. CONCLUSION: Women had greater barriers to CR participation than men. CR programs should be modified to address women's needs. Home-based CR tailored to women's exercise needs and preferences should be considered.

Inhalation exposure to crude oil vapor induces behavioural deficits by increasing oxidative stress and histopathological changes in rat hippocampus: Quercetin therapeutic approach.

OBJECTIVE: Inhalation exposure to crude oil vapor (COV) and petroleum products is considered responsible for neurobehavioral toxicity in human and animal models. The antioxidant activity of quercetin (Que) and its derivatives are promising for protecting the hippocampus. This study aimed to evaluate the neuroprotective role of Que against COV-induced behavioral alterations and hippocampus damage. METHODS: Eighteen adult male Wistar rats were randomly divided into the following three groups (n = 6): the control, the COV, and the COV + Que group. The inhalation method was used to expose the rats to crude oil vapors for 5 h daily, and Que (50 mg/kg) was administered orally. After 30 days of treatment, the spatial working memory and anxiety levels were evaluated using the cross-arm maze and elevated plus maze (EPM), respectively. TUNEL assay and hematoxylin-eosin (H&E) staining were used to identify the necrosis, normal and apoptotic cells in the hippocampus. Moreover, the levels of oxidative stress biomarkers including malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (TAC) were investigated in the hippocampus tissue. RESULTS: The results indicated that exposure to COV was associated with a significant decrease in spatial working memory and activity of CAT, TAC, SOD, and GPx enzymes compared to the control (P < 0.05). Moreover, COV significantly increased the level of anxiety, MDA, and hippocampal apoptosis (P < 0.05). The simultaneous administration of quercetin along with exposure to COV improved the behavioral alterations, activity of antioxidant enzymes, and hippocampal apoptosis. CONCLUSIONS: These findings suggest that quercetin prevents COV-induced hippocampal damage by enhancing the antioxidant system and preventing cell apoptosis.

Potential protective effect of quercetin on the male reproductive system against exposure of Wistar rats to crude oil vapor: Genetic, biochemical, and histopathological evidence.

Toxic compounds in crude oil vapor (COV), including polycyclic aromatic hydrocarbons (PAHs), are associated with adverse effects on reproduction in living organisms. Quercetin (QT) is the most plentiful flavonoid in vegetables and fruits, with antioxidant activities. This study aimed to evaluate the protective role of QT on testicular toxicity induced by COV. Twenty-four adult male Wistar rats were randomly divided into four groups (n = 6) including control, quercetin (QT) (50 mg/kg), crude oil vapors (COV), and COV + QT. The inhalation method was used to expose the rats to crude oil vapors for 5 h daily, and QT was administered orally. After 30 days, the rats were euthanized, then, the testes were removed for gonadosomatic index (GSI), sperm parameters, H&E staining, the activity of the antioxidant enzymes, and apoptotic gene expression assessments. The COV statistically significantly (P < 0.05) reduced GSI, sperm count, motility, viability, and sperm normal morphology, histological indexes, and antioxidant enzyme activities than control. Also, COV statistically significantly (P < 0.05) increased the expression of caspase-3, p-53, and Bax genes and decreased Bcl-2 gene expression. Co-administration of QT + COV caused a statistically significant (P < 0.05) decrease in Bax gene expression and increased antioxidant enzyme activities, Bcl-2 gene expression, and reproductive parameters than the COV group. Based on the results of this study, it appears that crude oil vapor causes side effects on male reproduction. Yet, quercetin has the potential to reduce the side effects of crude oil vapor on the male reproductive system.

Early Miocene larger benthic foraminifera from the northwestern Tethyan Seaway (NW Iran): new findings on Shallow Benthic Zone 25.

The lower Miocene marine units of NW Iran (Hamedan-Miandoab area) were studied to establish a high-resolution biostratigraphy framework in the context of European standard biozonation (SBZ zonal scheme). The units are dominated by larger and small benthic foraminifera together with coralline algae and corals. Due to its position in the Tethyan Seaway between the Mediterranean and Indo-Pacific, the investigated area has high significance in palaeobiogeography. Seven species of benthic foraminifera were described, including Miogypsina globulina, Amphistegina bohdanowiczi, Elphidium crispum, Elphidium sp. 1, Borelis curdica, Austrotrillina asmariensis, and Peneroplis thomasi. The occurrence of Miogypsina globulina in the shallow-marine carbonates of the studied sections documents the Burdigalian SBZ 25 Zone. Austrotrillina asmariensis and Peneroplis thomasi occur with M. globulina. Simultaneously, their stratigraphical range is extended to the Burdigalian in the Middle East. Based on the discovery of lower Miocene deposits in the Hamedan-Miandoab area (previously mapped as Oligocene-Miocene units), the stratigraphy of northwest Central Iran is revised. The biostratigraphic results also present a more precise chronology for the marine transgression in the study area, initiating in the late early Miocene. The coralline algal assemblages and the abundance and diversity of larger benthic foraminifera indicate that shallow-marine Qom Formation got deposited in tropical to subtropical warm waters.

Protective effect of vitamin E on sperm quality and in vitro fertilizing potential and testosterone concentration in polyvinyl chloride treated male rats.

Polyvinyl chloride (PVC) has toxic effects through the induction of oxidative stress in the body and testicles. Vitamin E (Vit E) is a dietary compound that functions as an antioxidant scavenging toxic free radicals. The present study aimed to probe the protective effect of Vit E against PVC-induced reprotoxicity in male rats. In this experimental study, 24 male rats were randomly divided into four groups (n=6) including control, Vit E (150 mg kg-1 per day; orally), PVC (1000 mg kg-1 per day; orally) and PVC + Vit E. After 40 days, rats were euthanized and epididymal sperms characteristics, embryo development and malondialdehyde (MDA) and testosterone levels were examined. The PVC decreased sperm count, motility and viability as well as testosterone level and increased sperms with damaged chromatin in comparison with controls. Also, the percentages of fertilization, two-cell embryos and blastocysts as well as MDA levels were decreased in PVC-treated rats. However, Vit E improved PVC-induced alterations in aforesaid parameters. The results indicated that PVC can reduce fertility potential in male rats probably through androgen and sperm quality and quantity reductions, while Vit E can exert protective effects in PVC-related reproductive toxicities.

Health Risk Assessment for Human Exposure to Trace Metals and Arsenic via Consumption of Hen Egg Collected from Largest Poultry Industry in Iran.

The relative contribution of foodstuffs to intake of heavy metal is still equivocal, and thus, available data are rare. Here, the concentration of ten heavy metals and arsenic were measured in the content of hen egg from five strains of commercial laying hens to evaluate contamination level and potential health risk among local and international consumers in Iran. The estimated daily intake (EDI) of all heavy metals was significantly lower than the provisional tolerable daily intake (PTDI) and respective tolerable daily intake (TDI). The non-carcinogenic and carcinogenic risk values for eating eggs were in the safe range for all consumers, indicating that the no health risk for consumers due to the daily intake of either arsenic or heavy metals. Therefore, intake of heavy metal exposure through the consumption of commercial egg product in Iran is completely safe for both national and international consumers.

The impact of strain and feed intake on egg toxic trace elements deposition in laying hens and its health risk assessment.

The impact of strain or feed intake on food trace elements and its health risk assessment is still ambiguous, and therefore, available facts are rare. Therefore, this study aimed to assess the effects of both strains and feed intake on trace elements depositions to egg, toxic heavy metals concentration, and health risk assessment of egg consumption. In the current cross-sectional study, the selected strains, including Shaver White, Hy-Line W36, Bovanse White, Lohman LSL-Lite, and Native laying hens of Khorasan Razavi province, were examined. A total number of 50 samples of eggs and 15 samples of their feed was purchased from poultry farms. Yolk and white were separately analyzed. Sample preparation was performed by wet digestion followed by Inductively Coupled Plasma Mass Spectrometry. Trace metals Pb, As, Cd, Hg, Cr, and Ni were detected. Statistical analysis was performed in Stata11.2 portable software. Although there was a significant difference in strains and feed, no significant difference was observed in trace elements in egg contents (weight of egg white and egg yolk). Mercury concentration in all the samples was below the instrument detection limit. In this study, the target hazardous quotients were below one for all trace elements. Therefore, Iranian does not experience the adverse health effects due to the consumption of egg.

Revised version of the Cenozoic Collision along the Zagros Orogen, Insights from Cr-spinel and Sandstone Modal Analyses.

Geoscientists have always considered the Neyriz region, located along the Zagros Suture Zone, an important area of interest because of the outcrops of Neotethys ophiolitic rocks. We carried out a modal analysis of the Cenozoic sandstones and geochemistry of the detrital Cr-spinels at Neyriz region in order to determine their provenance and tectonic evolution in the proximal part of Zagros Basin. Our data shows a clear change in provenance from the Late Cretaceous onwards. As from the Late Cretaceous to Eocene, lithic grains are mostly chert and serpentinite; and higher Cr# values of the detrital Cr-spinel compositions indicate that they originate from the fore-arc peridotites and deposited in an accretionary prism setting during this period. From the Late Oligocene to the Miocene periods, volcaniclastic and carbonate lithic grains show an increasing trend, and in the Miocene, metasedimentary lithic grains appear in the sediments. Ophiolite obduction caused a narrow trough sub-basin to be formed parallel to the general trend of the Zagros Orogeny between the Arabian and Iranian plates in Oligocene. From the Miocene onwards, the axial metamorphic complex belt was uplifted in the upper plate. Therefore, the collision along the Zagros Suture Zone must have occurred in the Late Oligocene.

Optimization of diazinon biodegradation from aqueous solutions by Saccharomyces cerevisiae using response surface methodology.

Diazinon is an organophosphate compound that inhibits the activity of acetylcholinesterase. Standards of the World Health Organization and Environmental Protection Agency for diazinon concentration in water are 0.1 and 9 × 10-6 mg/L, respectively. The aim of this study was the optimization of diazinon biodegradation from aqueous solutions by Saccharomyces cerevisiae using the response surface methodology (RSM). Harvested cells of S. cerevisiae were locally purchased from the Iranian Research Organization for Science and Technology. To obtain the optimum condition for diazinon biodegradation using RSM, input parameters included the initial concentration of diazinon (0.01-10 mg/L), concentration of S. cerevisiae (0.5-5%), pH (4-10), and retention time (1-30 h). The research study had a central composite design where one of the methods was RSM. According to the results, the observed values of the removal efficiency of diazinon were variable in the range of 23-96. The highest removal rate was obtained as 96% under the initial diazinon concentration of 2.5 mg/L, S. cerevisiae concentration of 3.88%, pH of 5.5, and retention time of 22.75 h. The results displayed that the removal efficiency of diazinon had a direct relationship with the concentration of S. cerevisiae and retention time, and an inverse relationship with pH and the initial concentration of diazinon. We can conclude that S. cerevisiae has the ability to remove diazinon with the lowest cost and a high efficiency.

Cardiovascular, respiratory, and total mortality attributed to PM2.5 in Mashhad, Iran.

Poor air quality is one of the most important environmental problems in many large cities of the world, which can cause a wide range of acute and chronic health effects, including partial physiological disorders and cardiac death due to respiratory and cardiovascular diseases. According to the latest edition of the national standard for air quality, maximum contamination level is 15 µg/m(3) per year and 35 µg/m(3) per day. The aim of this study was to evaluate cardiovascular, respiratory, and total mortality attributed to PM2.5 in the city of Mashhad during 2013. To this end, the Air Q model was used to assess health impacts of PM2.5 and human exposure to it. In this model, the attributable proportion of health outcome, annual number of excess cases of mortality for all causes, and cardiovascular and respiratory diseases were estimated. The results showed that the number of excess cases of mortality for all causes and cardiovascular and respiratory diseases attributable to PM2.5 was 32, 263, and 332 µg/m(3), respectively. Moreover, the annual average of PM2.5 in Mashhad was obtained to be 37.85 µg/m(3). This study demonstrated that a high percentage of mortality resulting from this pollutant could be due to the high average concentration of PM2.5 in the city during 2013. In this case, using the particle control methods, such as optimal use of fuel, management of air quality in urban areas, technical inspection of vehicles, faster development of public transport, and use of industrial technology can be effective in reducing air pollution in cities and turning existing situations into preferred ones.

Regulation of the cardiac L-type Ca2+ channel by the actin-binding proteins alpha-actinin and dystrophin.

The actin-binding proteins dystrophin and alpha-actinin are members of a family of actin-binding proteins that may link the cytoskeleton to membrane proteins such as ion channels. Previous work demonstrated that the activity of Ca2+ channels can be regulated by agents that disrupt or stabilize the cytoskeleton. In the present study, we employed immunohistochemical and electrophysiological techniques to investigate the potential regulation of cardiac L-type Ca2+ channel activity by dystrophin and alpha-actinin in cardiac myocytes and in heterologous cells. Both actin-binding proteins were found to colocalize with the Ca2+ channel in mouse cardiac myocytes and to modulate channel function. Inactivation of the Ca2+ channel in cardiac myocytes from mice lacking dystrophin (mdx mice) was reduced compared with that in wild-type myocytes, voltage dependence of activation was shifted by 5 mV to more positive potentials, and stimulation by the beta-adrenergic pathway and the dihydropyridine agonist BAY K 8644 was increased. Furthermore, heterologous coexpression of the Ca2+ channel with muscle, but not nonmuscle, forms of alpha-actinin was also found to reduce inactivation. As might be predicted from a reduction of Ca2+ channel inactivation, a prolonging of the mouse electrocardiogram QT was observed in mdx mice. These results suggest a combined role for dystrophin and alpha-actinin in regulating the activity of the cardiac L-type Ca2+ channel and a potential mechanism for cardiac dysfunction in Duchenne and Becker muscular dystrophies.