RESUMO
A retrospective investigation of pig tissue samples from different classical swine fever virus (CSFV) outbreaks was undertaken employing RT-PCR for possible coinfection with other swine viruses. Four samples from three different outbreaks were found to be coinfected with Japanese encephalitis virus (JEV). Phylogenetic analysis was done based on complete E gene sequenced from all four coinfected samples. This revealed a new introduction of a divergent subgroup of JEV genotype I in India. This is the first report of detection of coinfection of JEV and CSFV in pigs and the first incidence of JEV genotype I in pigs in India.
Assuntos
Vírus da Febre Suína Clássica , Peste Suína Clássica , Coinfecção , Vírus da Encefalite Japonesa (Espécie) , Encefalite Japonesa , Animais , Peste Suína Clássica/epidemiologia , Vírus da Febre Suína Clássica/genética , Coinfecção/epidemiologia , Coinfecção/veterinária , Surtos de Doenças/veterinária , Vírus da Encefalite Japonesa (Espécie)/genética , Encefalite Japonesa/epidemiologia , Encefalite Japonesa/veterinária , Genótipo , Índia/epidemiologia , Filogenia , Estudos Retrospectivos , Suínos , Doenças dos Suínos/epidemiologiaRESUMO
Mithun is a strongly built hill animal of Southeast Asia and plays an important role in the socio-economic and cultural life of the tribal population. Setaria digitata isolated from peritoneal cavity of mithun both from Arunachal Pradesh and Nagaland were characterized based on conserved region of 12SrDNA, 28SrDNA and ITS-2 and mitochondrial gene CoxI. Based on sequence analysis, it was found to be 99% similarity with Srilankan isolate of S. digitata.