Cd2+-Specific Fluorescence Response of Methoxy-Substituted N,N-Bis(2-quinolylmethyl)-2-methoxyaniline Derivatives.

The N3O1 tetradentate ligand, TriMeOBQMOA (N,N-bis(5,6,7-trimethoxy-2-quinolylmethyl)-2-methoxyaniline), was developed as a Cd2+-specific fluorescent sensor. The structure of TriMeOBQMOA is half of TriMeOBAPTQ (N,N,N',N'-tetrakis(5,6,7-trimethoxy-2-quinolylmethyl)-1,2-bis(2-aminophenoxy)ethane), which is a tetrakisquinoline derivative of the well-known calcium chelator BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid). The fluorescent Cd2+ selectivity of TriMeOBAPTQ (IZn/ICd = 5.3% in the presence of 3 equiv of metal ions in MeOH-HEPES buffer (9:1)) comes from the formation of fluorescent dinuclear cadmium (M2L) and nonfluorescent OH-bridged dizinc ((µ-OH)M2L) complexes. TriMeOBQMOA also exhibits excellent Cd2+ specificity in fluorescence enhancement (IZn/ICd = 2.3% in the presence of 5 equiv of metal ions in DMF-HEPES buffer (1:1, HEPES 50 mM, KCl 0.1 M, pH = 7.5)) via substantial formation of a highly fluorescent bis(µ-chloro)dinuclear cadmium complex ([Cd2(µ-Cl)2L2]2+), which is in equilibrium with the mononuclear Cd2+ complex ([CdLCl]+), and extremely poor stability of the TriMeOBQMOA-Zn2+ complex. The all-nitrogen derivatives of BQMOA and BAPTQ, namely, N,N-BQDMPHEN (N,N-bis(2-quinolylmethyl)-N',N'-dimethyl-1,2-phenylenediamine) and BPDTQ (N,N,N',N'-tetrakis(2-quinolylmethyl)-2,2'-(N,N'-dimethylethylenediamino)dianiline), respectively, and their methoxy-substituted derivatives were also prepared, and the fluorescent metal ion sensing properties are discussed.

Changes in the Health Condition after Using a Service Dog of a Person with Complete C5 Spinal Cord Injury: A Qualitative Single Case Study.

BACKGROUND: We report the changes in the health condition after using a service dog of a person with a complete C5 spinal cord injury (SCI). CASE: A 48-year-old married man began using a service dog after suffering a complete C5 SCI. We conducted a semi-structured interview based on the International Classification of Functioning, Disability, and Health (ICF) rehabilitation sets to describe the change in the participant's experience after using a service dog. The interview was converted into written form, and information regarding the changes in the participant's health condition was extracted. These data were categorized by similarity and then classified by the ICF conceptual framework. DISCUSSION: The participant experienced multiple changes in his health condition after using a service dog. These included the following factors from the ICF - "Body Function" category: "increased vitality and motivation," "emergence of self-affirmation," and "physical improvement." Health changes form the "Activities and Participation" category included: "emergence of new roles," "increased resilience," "expansion of moving area," "acquisition of activities incorporated with a service dog assist," "emergence of autonomous health management," "expansion of relation with other people," and "expansion of social activity." The participant experienced "reduction of psychological barriers," categorized under "Personal Factor," and "expansion of support environment," categorized under "Environmental Factor."