Noise accelerates embryonic development in a key crab species: Morphological and physiological carryover effects on early life stages.

Anthropogenic noise is considered one important global pollutant. The impact of noise on marine invertebrates has been less assessed. The present study evaluated the chronic effect of the motorboat noise obtained from a lagoon's soundscape, the natural habitat of the key crab Neohelice granulata, on its whole embryonic development, considering morphological and physiological carryover effects on embryos and hatched larvae. Results demonstrated that embryonic development was shortened under noise exposure. The effects on advanced embryos, larvae and adult females were: increased heartbeats and non-viable eggs, and decreased fecundity. Biochemical responses showed lipid peroxidation in embryos while antioxidant enzymes were activated in larvae and adults, indicating a counteracting effect related to the life stage. The negative effects on fitness offspring may imply ecological consequences at the population level. Results are discussed in terms of the ecosystem engineer species studied and the habitat, a MAB UNESCO Reserve lagoon, suggesting the urgent need to develop mitigation plans.

Effect of biological and anthropogenic habitat sounds on oxidative stress biomarkers and behavior in a key crab species.

Soundscapes are characterized by a combination of natural and anthropogenic sounds. This study evaluated the stress effect of biological and anthropogenic sounds characterizing a Man and Biosphere UNESCO wetland, by assessing the protein content, oxidative biomarkers, and behavior of a key crab species (Neohelice granulata), through a tank-laboratory experiment. Biological sounds corresponded to predators of N. granulata (fish and crustacean stimuli), while anthropogenic ones belonged to motorboat passages (boat stimulus). Biochemical results showed differences depending on the sound stimuli used and the crab tissue analyzed. Protein content was higher in hemolymph when crabs were exposed to fish and boat stimuli, and in gills when exposed to boat stimulus. The enzymatic activity in hemolymph showed a decreased GST (fish stimulus) and CAT (fish and boat stimuli) activity, in hepatopancreas a higher GST (crustacean stimulus) and CAT (crustacean and boat stimuli) activity was found, and in gills a higher CAT activity was also observed (crustacean and boat stimuli). Lipid peroxidation was higher only in hemolymph (fish and crustacean stimuli). Protein oxidation was higher in gills (fish stimulus) and hepatopancreas (crustacean stimulus). Behavioral analysis demonstrated that the crab locomotion activity diminished when exposed to diverse sound stimuli. Thus, both sound sources caused physiological and behavioral stress in this species. The results contribute important data to be used in the development of management plans considering the habitat importance in terms of biodiversity, the ecosystem services provided and the role of the studied species.

Are semi-terrestrial crabs threatened by human noise? Assessment of behavioural and biochemical responses of Neohelice granulata (Brachyura, Varunidae) in tank.

This study examined the effects of human lab-generated noise (sweep tone) on the behaviour and biochemistry of a semi-terrestrial crab (Neohelice granulata). The experiment was carried out in tanks equipped with video- and audio-recording systems on a total of seventy-eight specimens. In total, 42 experimental trials with sweep-tone exposure and control conditions were performed using crabs in single and group layouts. After a habituation period of 30 min, the locomotor and acoustic (sound signals emitted by the crabs) behaviours were monitored for 30 min. During this time, the animals in sweep-tone conditions were exposed to ascending sweeps in a bandwidth range of 2.5-25 kHz. Exposure to sweep-tone noise produced significant changes in the number of signals emitted, locomotor behaviours and plasma parameters, such as haemolymph total haemocyte count and glucose, lactate and total protein concentrations, revealing that human noise could represent a disturbance for this crustacean species.