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1.
J Water Health ; 16(3): 391-402, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29952328

RESUMO

We evaluate the behavior of a device designed to automatically divert and store the first flush of harvested rainwater in cisterns. The first phase (PI) was conducted with artificial precipitation in an experimental installation seeking to identify how many millimeters of rainwater should be diverted to preserve the rainwater quality. In the second phase (PII), we designed a PVC-pipe device to store the first millimeter of rainwater, and tested it in field (a rural area in Brazil) during two real rainfall events. In the third phase (PIII), the device and a hand pump were assayed for two years using eight cisterns in a rural area where people drink the rainwater. PI results indicated that the most significant pollution of the rainwater is flushed with the first millimeter of rain, and diversion promoted the removal of 98% and 100% of the total coliforms and Escherichia coli, respectively. The bacteriological behavior was maintained in the subsequent phases. The device was able to preserve the quality of the rainwater most of the time, satisfying drinking requirements for the parameters of turbidity and color. The satisfactory performance of the device was confirmed in the field, behaving as a sanitary barrier for rainwater quality protection.


Assuntos
Água Potável , Chuva , Microbiologia da Água , Purificação da Água/instrumentação , Abastecimento de Água , Bactérias , Brasil , Clima , Humanos , Purificação da Água/métodos , Qualidade da Água
2.
J Water Health ; 12(3): 513-25, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25252355

RESUMO

Harvesting rainwater is a common practice worldwide, particularly in areas with no access to a public water supply or insufficient groundwater reserves. More than two million people living in semiarid regions of Brazil consume rainwater stored in cisterns, and little information is available regarding the water quality. Despite the initial good quality of the rainwater, its harvest and storage can introduce contaminants that must be eliminated before consumption. To evaluate the influence of handling, cistern age and precipitation on the quality of harvested rainwater, we monitored seven cisterns in the semiarid Brazilian Northeast over 4 years. Microbial and physicochemical parameters were monitored once a month, and denaturant gradient gel electrophoresis (DGGE) was performed at the end of the monitoring period. Coliform bacteria were detected in 100% of samples, while Escherichia coli were observed in 73.8%. The alkalinity and conductivity were the highest for the recently built cisterns due to the dissolution of construction materials. The DGGE of the 16S r DNA did not reveal the presence of E. coli. Instead, DGGE bands sequencing indicated that species primarily affiliated with Alphaproteobacteria were present in all cisterns, indicating the presence of microbial ecosystems capable of purifying and stabilizing the stored rainwater.


Assuntos
Bactérias/isolamento & purificação , Biodiversidade , Água Potável/microbiologia , Qualidade da Água , Bactérias/classificação , Brasil , DNA Bacteriano/genética , Eletroforese em Gel de Gradiente Desnaturante , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
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