Hollow carbon nanocapsules-based nitrogen-doped carbon nanofibers with rosary-like structure as a high surface substrate for impedimetric detection of Pseudomonas aeruginosa.

The design of hollow mesoporous carbon-based materials has attracted tremendous attention, due to their sizeable intrinsic cavity to load specific chemical and unique physical/chemical properties in various applications. Herein, we have established an effective strategy for the preparation of novel hollow carbon nanocapsules-based nitrogen-doped carbon nanofibers (CNCNF) with rosary-like structure. By embedding ultrafine hollow carbon nanocapsules into electrospun polyacrylonitrile (PAN) skeleton, the as-designed composite CNFs were carbonized into hierarchical porous CNFs, consisted of interconnected nitrogen-doped hollow carbon nanocapsules. Due to its individual structural properties and unique chemical composition, the performance of CNCNF was evaluated in aptasensor application via the detection of Pseudomonas aeruginosa (PA). Under optimized conditions, the aptasensor based on CNCNF has a detection limit of 1 CFU⋅mL-1 and a linear range from 101 CFU ⋅mL-1 to 107 CFU ⋅mL-1 (n = 3). Moreover, the designed aptasensor possesses high sensivity, high selectivity, low detection limit, and high reproducibility. These studies showed that the CNCNF material offers a wide variety of enhanced electrochemical features as an electrode material for aptasensor application.

Impedimetric aptasensor for Pseudomonas aeruginosa by using a glassy carbon electrode modified with silver nanoparticles.

An aptasensor is described for the ultrasensitive detection of Pseudomonas aeruginosa (P. aeruginosa). A glassy carbon electrode (GCE) was first modified by electrodeposition of silver nanoparticles (AgNPs). Immobilization of NH2-aptamer was covalently attached to the AgNP/GCE surface. The morphology, distribution and size of the sensor were characterized by field emission scanning electron microscopy. Cyclic voltammetry and electrical impedance spectroscopy were used to study conductivity of the aptasensor and the electrochemical properties. Detection of P. aeruginosa was carried out by evaluation of the charge transfer resistance after and before the adding of P.aeruginosa and by using the hexacyanoferrate redox system as an electrochemical probe. The impedance increases on going from 102 to 107 CFU·mL-1 concentrations of P. aeruginosa, and the detection limit is 33 CFU·mL-1 (for S/N = 3). The assay was successfully applied for the determination of P. aeruginosa in spiker serum samples. Graphical abstract Schematic representation of an impedimetric assay for Pseudomonas aeruginosa by using a [Fe(CN)6]3-/4- probe based on immobilization of amino-modified aptamer onto a glassy carbon electrode (GCE) modified with silver nanoparticles (AgNPs): Incubation with P. aeruginosa leads to an increase of the charge-transfer resistance.