Comparing carbon footprints of sheep farming systems in semi-arid regions of India: A life cycle assessment study.

Carbon foot prints (CFs) studies based on life cycle assessment between sheep farming systems and green house gases (GHG) emissions is one of the best indicators to quantify the amount of GHG emissions per kg of product. Therefore, a life cycle assessment (LCA) study was conducted for three different sheep farming systems i.e. intensive system (stall fed only), semi-intensive (grazing with supplementation) and extensive system (grazing only) under semiarid region of India to assess the carbon cost of sheep rearing. The total CFs were estimated to be 16.9, 15.8 and 17.1 kg CO2-eq in intensive, semi-intensive and extensive system of grazing indicating semi-intensive system to be most carbon (C) efficient. For 1kg mutton production in semi-intensive and intensive system, around 30% and 24% CFs were contributed from enteric fermentation and feed respectively, whereas, in extensive system, the contribution of enteric fermentation increased up to 50%. The carbon foot prints analysis gives an insight of carbon inputs used but the amount of CO2 sequestered in soil making LCA a holistic approach for estimating GHG emissions from livestock.

Supplementation of Moringa oleifera leaf concentrate pellets on nutrient utilization, antioxidant status, and reproductive performance of prolific ewes during extreme summer months in semi-arid tropical conditions.

A feeding trial was conducted for a period of 60 days during extreme summer months to observe the effect of supplementation of Moringa oleifera leaves containing concentrate pellets on nutrient utilization, antioxidant status, and reproductive performance in Avishaan ewes reared under semi-arid condition. Forty adult non-pregnant cyclic ewes (2-3 years, 31.8 ± 0.81 kg body weight) were selected and randomly allocated into 2 groups of 20 animals each, viz., G-I (control) and G-II (treatment). The ewes were grazed on natural pasture for 8 h, offered ad libitum Cenchrus ciliaris hay after grazing and concentrate pellets @ 300 g/animal/day. The ewes in G-I were offered conventional concentrate pellets, whereas G-II ewes were offered concentrate pellets containing 15% Moringa leaves. The mean temperature humidity index during the period of study was 27.5 ± 0.3 and 34.6 ± 0.4 at 0700 h and 1400 h, respectively, indicating severe heat stress. Nutrient intake and utilization were comparable between the two groups. The antioxidant status was higher in G-II ewes as the values of catalase, superoxide dismutase, and total antioxidant capacity were higher (P < 0.05) in G-II ewes compared to G-I. The conception rate was higher (100%) in G-II ewes than G-I ewes (70%). Multiple birth percentage was 77.8% in G-II ewes, and it was comparable with the herd average of Avishaan (74.7%). However, ewes in G-I group exhibited a marked decline in multiple birth percentage (28.6%) than the normal herd average. Hence, it can be concluded that inclusion of Moringa oleifera leaves in feeding of prolific Avishaan ewes improved their antioxidant status resulting in optimum reproductive performance during stressful summer months.

Effect of supplementation of roasted oilseeds and phytochemical-rich herbages on nutrient utilization, methane emission, and growth performance in finisher lambs.

Ruminal methanogenesis is a major contributor to global environmental pollution in the agriculture sector. Dietary intervention modestly abates enteric methane emissions in ruminants. Therefore, the present experiment was conducted to evaluate the combined effect of dietary oilseeds and phytochemical-rich herbages on enteric methane emission, growth performance, and nutrient utilization in lambs. Forty-eight finisher Malpura lambs were divided into 4 groups (RSZ, RSP, RSLZ, and RSLP) of 12 each in a factorial design. Lambs were fed ad libitum concentrate formulated with roasted soybean (RS) or roasted soybean plus linseed (RSL) along with Ziziphus nummularia (Z) or Prosopis cineraria (P) leaves as roughage sources. Variation in the source of roughage significantly affected feed intake, and it was higher (P < 0.05) in lambs fed Prosopis cineraria leaves (RSP and RSLP). The average daily gain was improved (P < 0.05) by 28.6 and 25.0% in lambs fed Prosopis cineraria, i.e., RSP and RSLP respectively, than those fed Ziziphus nummularia leaves irrespective of concentrate diets. Overall, microbial nitrogen synthesis (MNS) was higher (P < 0.05) in lambs fed roasted soybean (RS) than roasted soybean plus linseed (RSL); however, combination of Prosopis cineraria with both the concentrate diets increased MNS, than the combination of roasted oilseeds and Ziziphus nummularia. No significant interaction was observed in the concentration and proportion of volatile fatty acids by feeding combination of roasted oilseed and tree leaves; however, the proportion of acetic and propionic acid was higher (P < 0.05) in the RSL group compared to RS. Methane emission per kg dry matter intake was reduced (P < 0.05) by feeding Prosopis cineraria leaves regardless of concentrate mixture. As a result, the loss of metabolizable energy through methane emission was lowered by 0.7 and 4.6% when Prosopis cineraria leaves were combined with roasted soybean (RSP) and roasted soybean plus linseed (RSLP), respectively. From the present findings, it can be concluded that combination of Prosopis cineraria leaves either with roasted soybean or roasted soybean plus linseed reduced enteric methane emissions more effectively than Ziziphus nummularia leaves, and the energy saved was transformed into higher body weight gain and improved feed conversion ratio.

Use of Moringa oleifera leaves (sole or combined with concentrate) in rabbit feeding: Effects on performance, carcass characteristics and meat quality attributes.

Sixty Chinchilla rabbits (28 days old) were divided into three equal groups (n = 20). Rabbits in MOL0 (control) were fed pellets containing 700 g cowpea hay/kg pellet as forage source, whereas rabbits in MOL700 and MOL950 were fed pellets containing 700 and 950 g moringa leaves/kg feed pellets, respectively. Average daily gain and feed conversion ratio was comparable in MOL700 and MOL0, however, it was higher in MOL950. Among the carcass traits, dressing percent was higher while, chilling loss was lower in MOL700 treatment. The Longissimus thoracis muscle of rabbits fed moringa leaves containing pellets (MOL700 and MOL950) had lower saturated fatty acid content, higher C18:3n-3 and total n-3 fatty acids along with lower thrombogenic index value. Hence, incorporating moringa leaves at 70% level is beneficial in terms of improved growth performance and functional attributes of meat than diet- containing sole moringa leaves.

Growth performance, rumen fermentation and economic analysis of Malpura lambs raised on milk replacer at different weaning age under semiarid conditions.

Pre-weaning nutrition has significant influence on post-weaning performance of lambs. Strategic weaning management on milk replacer allowance at this phase can be a promising approach for harvesting desired carcass trait in finisher phase. Therefore, present experiment aimed economizing pre-weaning weight gain in lambs by feeding milk replacer and subsequently minimizing weaning age. A study of 90 days duration was conducted to determine the production performance, nutrient utilization and rumen fermentation of lambs on milk replacer allowance at different weaning age. Sixty-six Malpura lambs (10 days of age) were randomly divided into 3 equal groups. Lambs in control group were allowed conventional free suckling of dam up to 90 day of age, whereas lambs in the two treatment groups, that is W60 and W90 were offered milk replacer and weaned at 60 and 90 days, respectively. Lambs in all groups were offered ad-libitum creep mixture, Ziziphus nummularia leaves, Vigna unguiculata hay and fresh Ailanthus excelsa leaves. Overall weight gain was 12.7 per cent higher (p < 0.05) in W90 than control whereas in W60 group it was statistically comparable with both control and W90 groups. Overall average daily gain was 12.1 per cent higher (p < 0.05) in W90 group as compared to control. In case of nutrient digestibility, there was significant depression of dry matter (DM), organic matter (OM), crude protein (CP) and neutral detergent fibre (NDF) by 16.9, 17.2, 9.3 and 32.0 per cent in W60 than control; however, in W90, CP digestibility was higher (p < 0.05) than control and W60. Considering economic analysis, the net return obtained from W90 (90 days weaning plus milk replacer allowance) was more 20.0 per cent higher than control. Therefore, it can be concluded that lamb rearing on milk replacer and weaning at 90 days have a sustained positive effect on lambs' production performance and is more profitable than the practice of milk replacer allowance and early weaning in semiarid conditions.

Effect of Boron Supplementation on Nutrient Utilization and Productive Performance of Peripartum Murrah Buffaloes.

Boron (B) has been established as a beneficial micronutrient for some farm animals. However, its impact on coping negative energy balance during transition and subsequent production performance needs critical perusal. Therefore, the present study was undertaken to determine the effect of B supplementation on productive performance, apparent nitrogen (N), and mineral utilization in peripartum Murrah buffaloes. Thirty advanced pregnant buffaloes (60 days prior to expected date of calving) were allocated into three groups, i.e. control, B-200, and B-400, and supplemented with pharmacological concentration of B at 0, 200, and 400 ppm, respectively, from 45 days prior to expected date of calving till 120 days post calving. Two metabolism trials were conducted (with animals having similar expected date of calving), one during prepartum (30 days prior to the expected date of calving) and another during postpartum (90 days post calving) phase to evaluate nutrient utilization and balance of N as well as minerals. Results revealed that B supplementation decreased (P < 0.05) overall plasma nonesterified fatty acids (NEFA), whereas plasma level of ß-hydroxybutyric acid, insulin, triglyceride, and glucose did not differ (P > 0.05) among the groups. Dry matter intake, nutrient digestibility, and N balance remained unaffected with B supplementation. Higher (P < 0.05) apparent absorption coefficient for magnesium (Mg) and zinc (Zn) was observed in pregnant animals, while during lactation, absorption coefficient of calcium, Mg, and Zn was increased (P < 0.05) in B-supplemented groups. Hence, it can be concluded that alteration in NEFA concentration and variation in apparent absorption coefficient of minerals with B supplementation suggest its role in energy and mineral metabolism. However, further investigations are required to determine the underlying mechanism of action for the present findings.

Effect of supplementing Leucaena leucocephala leaves alone or in conjunction with malic acid on nutrient utilization, performance traits, and enteric methane emission in crossbred calves under tropical conditions.

Dietary strategies aiming at minimizing enteric methane (CH4) emission in ruminants are of practical interest from nutritional, economical, and environmental point of view. The present study evaluated the effects of supplementing Leucaena leucocephala leaves either alone or in conjunction with malic acid on nutrient utilization, growth performance, and enteric CH4 emission in crossbred cattle fed wheat straw and concentrate-based diet under tropical conditions. Eighteen crossbred (Karan-Fries) calves were randomly allocated into 3 groups: G-I (control)-fed wheat (Triticum aestivum) straw and concentrate mixture in the ratio 50:50; G-II-fed wheat straw, concentrate mixture, and Leucaena leucocephala leaves in the ratio 45:45:10; and (3) G-III-fed similar diet like G-II with an additional supplementation of 1% malic acid on dry matter intake basis. Experimental feeding spanning 90 days included a 7-day metabolism trial and CH4 quantification study by sulfur hexafluoride tracer technique. Results revealed no significant effect of dietary treatments on dry matter intake (DMI) and digestibility of nutrients, except neutral detergent fiber (NDF) digestibility which was 5.5% higher (P < 0.05) in G-III as compared to control. Further, nitrogen (N) metabolism, rumen microbial protein synthesis, and growth performance remained similar among the treatments. No significant effect was also observed for enteric CH4 emission (expressed as g/day and g/kg DMI) in calves supplemented with Leucaena leucocephala leaves and malic acid. Therefore, the present findings depict modest improvement in fiber digestibility with no encouraging effect in mitigating enteric CH4 in growing cattle calves by supplementing Leucaena leucocephala leaves alone or with malic acid within the selected levels.

Isolation and characterization of anaerobic bacteria with fiber degradation potential from faeces of Boselaphus tragocamelus grazing on semi arid Indian conditions.

Hindgut of wild ruminants harbours diversified anaerobic bacteria with promising fiber degrading ability. Fibrolytic enzyme activity is strongly influenced by diet and host species which till date remains unexplored for harnessing their optimum benefits. The present study was conducted to isolate and characterize fiber degrading anaerobic bacteria from faeces of wild blue bull (Boselaphus tragocamelus) inhabiting in semiarid regions. A total of 167 isolates were obtained from 85 faecal samples using M-10 medium, on the basis of clear zones formed on Congo red plates 20 isolates were selected and designated as NLG1-20 for microscopic and biochemical characterization. Further, molecular confirmation was done by PCR analysis with universal 16S rDNA primers. All isolates were obligatory anaerobes except, NLG4, NLG19 and NLG20. Majority of the sugars tested were utilized by most of the isolates except arabinose. Fibrolytic enzyme activities revealed that NLG1 had highest endoglucanase activity, NLG13 had highest exoglucanase activity while NLG8 showed maximum xylanase activity. In case of FPase assay, highest and lowest values were observed in isolate NLG11 (8.96 U/mL) and NLG8 (5.58 U/mL), respectively. Phylogenetic analysis of the isolates revealed a highly diverse group of microbes mainly belonging to the family Paenibacillaceae which have not been previously characterized in ruminants for fiber degradation. Therefore, results obtained in the present study indicated that the screened isolates showed promising fiber degrading potential in terms of filter paper assay and fibrolytic enzyme activity which can be explored further for improving lignocellulose digestibility in ruminants as an additive.

Utilization of fruit and vegetable waste as an alternative feed resource for sustainable and eco-friendly sheep farming.

Globally, 10-20% of horticultural wastes are disposed in landfills leading to environmental pollution. Recycling these wastes as animal feedstuff will lessen food-feed competition and minimize environmental hazards. The present study was undertaken to determine the nutritional quality of fresh fruit and vegetable waste (F&VW) and their dietary inclusion on nutrient utilization, antioxidant status, greenhouse gases (GHG) emissions and potable water sparing efficacy in sheep. Three dietary combinations were formulated i.e. control (C):70% Cenchrus ciliaris hay +30% concentrate mixture (CM), diet with fruit waste (FWD):70% Cenchrus ciliaris hay +20% CM +10% FW and diet with vegetable waste (VWD):70% Cenchrus ciliaris hay +20% CM +10% VW for in vitro and in vivo evaluation of these wastes as potential livestock feed. Twenty-one adult ewes were allocated into 3 groups C, FWD and VWD and fed on the above three diets. Dry matter and crude protein digestibility were significantly enhanced by 5.5 and 7.2%; 7.3 and 7.6% in F&VW supplemented groups, respectively, without affecting feed intake. Plasma total antioxidant capacity (TAC) was improved by 32.2 and 26.3% in F&VW supplemented groups. Inclusion of F&VW biomass reduced annual methane (CH4) and nitrous oxide (N2O) emissions (kg CO2eq/sheep) by 3.12 and 4.55%; 15.18 and 14.92% and thus contributed to lowering of global warming potential by 4.00 and 5.27%, respectively. Furthermore, there was a net reduction of potable water consumption by 21.78 and 13.92% in F&VW supplemented groups, respectively. Therefore, it can be concluded that F&VW can be a potential feedstuff for ruminants and its efficient reuse would minimize environmental impacts associated with disposal of such waste in the landfills.

Comparative effect of zinc supplementation by hydroxy and inorganic sources on nutrient utilisation, mineral balance, growth performance and growth biomarkers in pre-ruminant calves.

Zinc (Zn), an important microelement in ruminant diet, plays a critical role in various enzymes, hormones and functional proteins involved in nutrient metabolism. The present study was conducted to assess the effect of zinc hydroxychloride (Zn5(OH)8Cl2·H2O [ZnOHCl] and zinc sulphate (ZnSO4) on nutrient utilisation, mineral metabolism and biomarkers pertaining to growth performance in pre-ruminant crossbred calves. Twenty-four crossbred calves [body weight (BW); 31.03 ± 4.30 kg; age 10 d] were randomly allocated to four treatment groups (n = 6), i.e. no supplementation of Zn (0 mg/kg dry matter [DM]), 80 mg/kg DM Zn as ZnSO4(ZnS-80), 40 mg/kg DM Zn as ZnOHCl (ZnH-40) and 80 mg/kg DM Zn as ZnOHCl (ZnH-80) for 90 d experimental period. Results showed that dietary Zn supplementation improved (p < 0.05) feed intake, BW, average daily gain, heart girth, body length, plasma growth hormone, insulin-like growth factor and thyroxin concentration; however, nutrient digestibility remained unaffected among the groups. Addition of Zn increased (p < 0.05) Zn retention and plasma Zn concentration without affecting  retention and plasma concentration of other minerals. Retention of Zn was the highest in ZnH-80 group followed by ZnH-40, ZnS-80 and lowest in control group. Overall results of the present study indicate that regardless of sources and levels, Zn supplementation increased growth performance, plasma Zn concentration and hormones levels in pre-ruminant crossbred calves. However, supplementation of hydroxy Zn at 40 mg/kg DM had similar effect as produced by ZnSO4 or ZnOHCl at a supplementation level of 80 mg/kg DM. Therefore, from the present study it can be concluded that ZnOHCl can be used as a Zn source for pre-ruminant calves at a lower dose compared to ZnSO4.

Effect of Milk Fermented with Lactobacillus acidophilus NCDC15 on Nutrient Digestibility, Faecal Biomarkers and Immune Response in Murrah calves

Abstract In neonates, rapid change in diet imbalances gut health allowing colonization of opportunistic pathogens that confer harmful effects on animal health causing reduced digestion and malabsorption of nutrients. In this milieu, probiotic feeding can be a promising approach in promoting animal health and stabilization of gastrointestinal microbiota. Hence, the present study was designed to investigate the effect of Lactobacillus acidophilus NCDC15 enriched fermented milk on nutrient digestibility, faecal biomarkers and immune response in Murrah buffalo calves. Twenty-four, neonatal calves (5-7 days) were randomly allocated into four groups for 90 days. The control group (CT) was provided a basal diet of calf starter and green fodder (maize and jowar), without any probiotic fermented milk (PFM) supplementation. Basal diet was supplemented with probiotic fermented milk at 100, 200 and 300 mL/calf/day, in PFM100, PFM200 and PFM300 groups, respectively. Nutrient digestibility remained similar among the groups. Faecal acetate was higher (P<0.05) in PFM200 and PFM300, while, faecal butyrate was increased (P<0.05) with all levels of probiotic supplementation than control. Faecal Lactobacillus and Bifidobacterium count were increased (P<0.05) with a concomitant reduction in coliform population (P<0.05) among all the treatments. Cell-mediated and humoral immune response were higher (P<0.001) in PFM200 and PFM300 than CT. Overall, it can be concluded that inclusion of Lactobacillus acidophilus NCDC15 in the form of fermented milk upto 300 mL/calf/day improved immunity and faecal biomarkers in Murrah buffalo calves without any adverse effect on nutrient utilization which may positively impact growth performance in Murrah buffalo calves.

Boron supplementation in peripartum Murrah buffaloes: The effect on calcium homeostasis, bone metabolism, endocrine and antioxidant status.

BACKGROUND: Calcium homeostasis and immuno-endocrine system undergoes drastic changes in peripartum dairy animals and failure to adapt these physiological changes causes major impact on animal health as well as productivity. Boron (B), a newer trace element, influences calcium (Ca), phosphorus (P) and magnesium (Mg) metabolism as well as immune system by manipulating several hormones or enzyme systems. Present study was conducted to determine the effect of dietary B supplementation on Ca homeostasis, bone metabolism, endocrine and antioxidant status in peripartum Murrah buffaloes. METHODS: Thirty advanced pregnant Murrah buffaloes (8th month pregnant) were allocated into three groups based on their most probable producing ability (MPPA) and parity (n = 10 in each group) viz. B0, B200 and B400 and supplemented with 0, 200 and 400 ppm of B in the form of boric acid. Blood samples were collected at periodic intervals (-45, -30, -21, -15, -7, 0, 7, 15, 30, 60, 90 and 120 day relative to expected date of calving) and analysed for minerals concentration, hormonal profile, bone health biomarkers and antioxidant enzymes. RESULTS AND CONCLUSION: Boron supplementation at 200 and 400 ppm increased (p < 0.05) plasma Ca, Mg and osteocalcin (OCN) concentration during postpartum stage. Higher (p < 0.05) levels of plasma 25-hydroxy vitamin D3 were observed in both B supplemented groups as compared to B unsupplemented group irrespective of physiological stages. Plasma parathyroid hormone (PTH) and cortisol levels were lower (p < 0.05) in both B supplemented groups than B unsupplemented group, especially during postpartum stage. Whereas, plasma ferric reducing total antioxidant power (FRAP) activity was found to be higher (p < 0.05) in B supplemented groups as compared to B unsupplemented group. Furthermore, antioxidant enzymes (erythrocytic superoxide dismutase; SOD, catalase, glutathione peroxidase; GPx), plasma level of total immunoglobulins (TIg), bone specific alkaline phosphatase (BALP) and tartrate resistant acid phosphatase (TRAP) remained unaffected by dietary B supplementation. Overall, it can be concluded that supplementation of B at 200 ppm in the diet of peripartum Murrah buffaloes helped to induce metabolic adaptations for improving Ca homeostasis, bone metabolism and antioxidant status without much additional benefits at higher level used in the present study.

Utilization of byproducts of sheep farming as organic fertilizer for improving soil health and productivity of barley forage.

Wool is a biodegradable fiber, rich in nutrients and can be recycled in soil as a fertilizer for maximum benefits. The present study was planned with the hypothesis that waste wool could be used as a nutrient source or manure to forage crops and aim of this study was to identify practicable recycling options of sheep based wastes in agriculture. In our study, we have compared the effect of different sheep based organic wastes on soil health, crop productivity and water use. Application of waste wool in soil significantly improved the fertility status of soil, and considerable improvement was also observed in organic carbon and nitrogen, i.e. around 30.8 and 32.6% higher over control. The activities of soil enzymes were 10-30% and 3-20% higher in waste wool treatment as compared to control and sheep based manures, respectively. Application of waste wool not only improved soil health but produced 50% higher grain and dry fodder yield of barley over control. The improvement in physical properties of soil with waste wool resulted in higher water use efficiency of the system. Our study will help in distinguishing choices for safe use of organic wastes along with up gradation of soil health and crop water utilization, particularly in nutrient poor soils of arid and semi-arid region of India.

Perspectives for rare earth elements as feed additive in livestock - A review.

There is a need for newer feed additives due to legal prohibition on inclusion of growth promoting antibiotics in livestock diets in several countries due to antimicrobial resistance. In this context, rare earth elements (REE) have gained attention among animal nutritionists as potential growth promoters. Currently, several studies have reported better weight gain, milk production, egg laying capacity and feed conversion efficiency among different breeds of farm animals following supplementation with REE, with however largely inconsistent results. Furthermore, REE supplementation has also shown to improve ruminal fibrolytic and proteolytic activities as well as flavor of meat with negligible residues in edible tissue, however the mechanism behind this action is still unclear. According to existing research, due to their poor absorption and similarity with calcium REE might exert their action locally on gut microbial populations within the gastrointestinal tract (GIT). Moreover, REE have also shown anti-inflammatory, anti-oxidative as well as immune stimulating effects. The present review aims to broaden the knowledge about use of REE as feed additives for livestock and sum up efficacy of REE supplementation on performance and health of animals by comparing the findings. Till date, researches with REE have shown properties that make them a promising, new and safe alternative feed additive but further exploration is recommended to optimize effects and clarify discrepancy of various results before practical proposals can be drafted.