RESUMO
INTRODUCTION: HPA polymorphism has been associated with HCV presence and fibrosis progression in chronic hepatitis C. However, it is unknown if there is an association between HPA-1 polymorphism and hepatocellular carcinoma (HCC). Therefore, this study aimed to evaluate HPA-1 polymorphism in the presence of HCC. METHODS: PCR-SSP was used to perform HPA genotyping on 76 HCV-infected patients. RESULTS: There was no association between patients with and without HCC. There was significant difference in HPA-1 genotypic frequency distribution between HCC and F1/F2 fibrosis degree. CONCLUSIONS: The HPA-1a/1b polymorphism appears to be more associated with liver damage progression than with HCC presence.
Assuntos
Antígenos de Plaquetas Humanas/genética , Carcinoma Hepatocelular/virologia , Hepatite C Crônica/genética , Neoplasias Hepáticas/virologia , Carcinoma Hepatocelular/genética , Progressão da Doença , Feminino , Marcadores Genéticos , Genótipo , Hepatite C Crônica/virologia , Humanos , Integrina beta3 , Neoplasias Hepáticas/genética , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Prognóstico , Fatores de RiscoRESUMO
With the ever-progressing urbanization of human society comes lingering public issues, primarily those related to human health and the need to provide environments where the population can be routinely frequent. Also, the effects of climatic changes tend to aggravate such public health issues, mainly respiratory and cardiac symptoms. As such, analyzing and studying those environments can be crucial for public administrations in the decision-making processes. One such method of analysis involves using an air enthalpy index to profile and provide a picture of stress situations, helping expedite preventive measures and hospital logistics. With that, this study used the enthalpy index as a tool to create a picture of the effect of seasonality on chronic health issues, such as respiratory and cardiac symptoms, for the city of Botucatu, a city with a characteristic tropical altitude climate. It was possible to determine that the air enthalpy profile is consistent throughout the year near the estimated thermal comfort boundaries, with peaks outside in hotter months. The hospitalizations tend to be more numerous during the transition from hot to cold seasons. Simple correlation analysis, along with a t test, provided further insight into the issue, showing strong correlation for pneumonia with the maximum enthalpy variation, which can relate to the heat amount variation in a given location. Also, an agreement was observed between enthalpy and an established thermal sensation index, the heat index, meaning that air enthalpy can be a reliable and simple index for environmental thermal comfort analysis. That being said, further studies are necessary to confirm these findings, considering a longer time frame, more diverse hospitalization data, and other meteorological parameters, like wind speed and precipitation.
Assuntos
Sensação Térmica , Vento , Cidades , Conservação dos Recursos Naturais , Temperatura Alta , HumanosRESUMO
Abstract INTRODUCTION: HPA polymorphism has been associated with HCV presence and fibrosis progression in chronic hepatitis C. However, it is unknown if there is an association between HPA-1 polymorphism and hepatocellular carcinoma (HCC). Therefore, this study aimed to evaluate HPA-1 polymorphism in the presence of HCC. METHODS: PCR-SSP was used to perform HPA genotyping on 76 HCV-infected patients. RESULTS: There was no association between patients with and without HCC. There was significant difference in HPA-1 genotypic frequency distribution between HCC and F1/F2 fibrosis degree. CONCLUSIONS: The HPA-1a/1b polymorphism appears to be more associated with liver damage progression than with HCC presence.
Assuntos
Humanos , Masculino , Feminino , Antígenos de Plaquetas Humanas/genética , Carcinoma Hepatocelular/virologia , Hepatite C Crônica/genética , Neoplasias Hepáticas/virologia , Prognóstico , Marcadores Genéticos , Reação em Cadeia da Polimerase , Fatores de Risco , Carcinoma Hepatocelular/genética , Progressão da Doença , Hepatite C Crônica/virologia , Genótipo , Neoplasias Hepáticas/genética , Pessoa de Meia-IdadeRESUMO
INTRODUCTION:: Transforming growth factor beta 1 (TGFB1) and platelet-derived growth factor (PDGF) are the main cytokines related to hepatic fibrogenesis. METHODS:: RNA isolated from the platelets and hepatic tissue of 43 HCV carriers was used for quantitative polymerase chain reaction to determine TGFB1, PDGFA, and PDGFB RNA expression. RESULTS:: The mRNA expression of PDGFA in platelets was significantly lower in the group with advanced fibrosis than in the group with early-stage fibrosis. TGFB1 was more frequently expressed in platelets than in hepatic tissue, which was different from PDGFB. CONCLUSIONS:: A pathway mediated by overexpression of TGFB1 via PDGFA in megakaryocytes could be involved in the development of fibrosis.
Assuntos
Hepatite C Crônica/sangue , Cirrose Hepática/sangue , Fator de Crescimento Derivado de Plaquetas/análise , Proteínas Proto-Oncogênicas c-sis/sangue , Fator de Crescimento Transformador beta1/sangue , Adulto , Plaquetas/química , Feminino , Hepatite C Crônica/complicações , Humanos , Cirrose Hepática/virologia , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , RNA Mensageiro/análise , Índice de Gravidade de DoençaRESUMO
Abstract: INTRODUCTION: Transforming growth factor beta 1 (TGFB1) and platelet-derived growth factor (PDGF) are the main cytokines related to hepatic fibrogenesis. METHODS: RNA isolated from the platelets and hepatic tissue of 43 HCV carriers was used for quantitative polymerase chain reaction to determine TGFB1, PDGFA, and PDGFB RNA expression. RESULTS: The mRNA expression of PDGFA in platelets was significantly lower in the group with advanced fibrosis than in the group with early-stage fibrosis. TGFB1 was more frequently expressed in platelets than in hepatic tissue, which was different from PDGFB. CONCLUSIONS: A pathway mediated by overexpression of TGFB1 via PDGFA in megakaryocytes could be involved in the development of fibrosis.