Chemical analysis, antifungal and antimycotoxigenic activity of tetradenia riparia essential oil and crude extract.

Tetradenia riparia is known for its richness in essential oil which has been widely investigated due to its biological activities such as antimicrobial, insecticidal, trypanocidal, antimalarial and antioxidant. The objective of this work was to chemically analyze and evaluate the antifungal and antimycotoxigenic activity of the essential oil and the crude extract of leaves, flower buds and stems of T. riparia from the northwest region of the state of Paraná. The essential oil was obtained by hydrodistillation using a Clevenger-type apparatus. To obtain the crude extract, the leaves, flower buds and stems were pulverized and subjected to a dynamic maceration process using 70% v v-1 ethyl alcohol. Chemical analysis of the essential oil was performed by GC/MS, and chemical identification of the crude extract by UHPLC-ESI/qTOF. Antifungal activity (Rhizopus oryzae, Aspergillus flavus, Aspergillus ochraceus, Penicillium verrucosum and Fusarium graminearum) was performed by broth microdilution and the antimycotoxigenic assay was performed with A. ochraceus and P. verrucosum. Ochratoxin A was extracted by partition with chloroform and quantified by HPLC-FL. The oil yield was 0.29% for leaves, 0.34% for stems and 0.38% for flower buds, and the major compounds were fenchone, ß-caryophyllene, α-cadinol, 14-hydroxy-9- epi-caryophyllene, 9ß,13ß-epoxy-7-abietene, α-cadinol and 6-7-dehydroroyleanone. The main chemical compounds identified in the crude extract were terpenes, anthocyanins, flavonoids, tannins and phenolic acids. The minimum inhibitory concentration (MIC) of oils from leaves, flower buds and stems for the strains tested ranged from 0.87 mg mL-1 to 33.3 mg mL-1, while the minimum fungicidal concentration (MFC) ranged from 6.94 mg mL-1 and 33.3 mg mL-1. The MIC and MFC for ketoconazole, tebuconazole, sorbate and nitrite ranged from 0.05 to 33.3 mg mL-1. The oil and crude extract of leaves, stems and flower buds showed an inhibition of ochratoxin A production for P. verrucosum of approximately 100%.

Safflower seed supplementation in lamb feed: effects upon fatty acid profile and quality of meat patty formulations.

The aims of this study were to determine the fatty acid profile of meat from lambs fed with different levels of safflower seed (0%, 7.5%, and 15%) and, also, to compare the characteristics of the meat patties prepared from this lamb meat (LMP) with beef meat patties (BMP). The safflower seed-supplemented diet did not change the contents of polyunsaturated and unsaturated fatty acids, except for C22:1. All meat patty formulations were considered safe for consumption. The values of cooking yield, shrinkage, water absorption index, luminosity (L*), and redness (a*) were similar for the LMP and BMP tested. While the safflower seed-supplemented diet did not alter the moisture, ash, and protein levels of LMP, the lipid content was lower than that in BMP. The incorporation of 15% safflower seed into lamb feed contributed to promoting better sensory attributes of the meat patties. Most of the physicochemical properties evaluated were similar among LMP and BMP. However, to improve the sensory properties of the product, dietary supplementation with 15% safflower seed is recommended.

Characterization of lamb burgers with addition of flour from peach palm by-product / Caracterização de hambúrgueres de cordeiro com adição de farinha de subproduto de pupunha

There is an increased interest in the consumption of healthier and innovative meat products. This study aimed to produce lamb burgers by replacing pork fat by peach palm by-product flour ­ PPF. The burgers were produced with lambs that had a safflower seed-supplemented diet. The PPF was added at levels of 0% (H0), 3% (H3), 6% (H6) and 9% (H9) in the substitution of fat. The flour produced with peach palm by-product showed high fiber content (63.9%) and high capacity of water absorption (8.1 g.g-1) and oil absorption (3.8 g.g-1). The PPF addition increased the cooking yield (68.2 - 77.0%), moisture retention (38.5 - 43.4%) and dietary fiber content (3.2 - 7.0%) in relation to H0, while the color parameters were similar among the formulations. The sensorial attributes overall appearance and aroma did not differ (p>0.05) in the lamb burgers produced, but the highest scores for flavor, texture and purchase intention were observed for H0, H3 and H6. PPF addition to lamb burgers increased the hardness values, and it did not affect elasticity and cohesiveness. The microbiological parameters showed that the formulations are safe for consumption. Therefore, PPF can be considered a low-cost alternative to nutritional enrichment of lamb burgers, being the level of 6% the most recommended due to the sensorial acceptance.

Há um interesse crescente pelo consumo de produtos à base de carne mais saudáveis e inovadores. O objetivo deste estudo foi produzir hambúrgueres de cordeiro, substituindo a gordura de porco por farinha de subproduto de palmito pupunha - FPP. Os hambúrgueres foram produzidos com a carne obtida de cordeiros que tiveram dieta suplementada com sementes de cártamo. A FPP foi adicionada nos níveis de 0% (H0), 3% (H3), 6% (H6) e 9% (H9) em substituição à gordura. A farinha produzida com subproduto de palmito pupunha apresentou alto teor de fibras (63,9%) e alta capacidade de absorção de água (8,1 g.g-1) e absorção de óleo (3,8 g.g-1). A adição de FPP aumentou o rendimento após cocção (68,2 - 77,0%), a retenção de umidade (38,5 - 43,4%) e o teor de fibra alimentar (3,2 - 7,0%) em relação a amostra H0, enquanto os parâmetros de cor foram semelhantes entre as formulações. Os atributos sensoriais aparência geral e aroma não diferiram (p > 0,05) entre os hamburgueres produzidos, mas, as maiores notas para sabor, textura e intenção de compra foram observadas para H0, H3 e H6. A adição de FPP aos hambúrgueres de cordeiro aumentou os valores de dureza e não afetou a elasticidade e a coesão. Os parâmetros microbianos mostraram que as formulações são seguras para consumo. Portanto, a FPP pode ser considerada uma alternativa de baixo custo para o enriquecimento nutricional de hambúrgueres de cordeiro, sendo o nível de 6% o mais recomendado devido à aceitação sensorial.