Molecular and physiological responses to salt stress in salinity-sensitive and tolerant Hibiscus rosa-sinensis cultivars.

Ornamental plants are used to decorate urban and peri-urban areas, and during their cultivation or utilisation, they can be exposed to abiotic stress. Salinity is an abiotic stress factor that limits plant growth and reduces the ornamental value of sensitive species. In this study, transcriptomic analysis was conducted to identify genes associated with tolerance or sensitivity to salinity in two hibiscus (Hibiscus rosa-sinensis L.) cultivars, 'Porto' and 'Sunny wind'. The physiological and biochemical parameters of plants exposed to 50, 100, or 200 mM NaCl and water (control) were monitored. Salinity treatments were applied for six weeks. After four weeks, differences between cultivars were clearly evident and 'Porto' was more tolerant than 'Sunny wind'. The tolerant cultivar showed lower electrolyte leakage and ABA concentrations, and higher proline content in the leaves. Accumulation of Na in different organs was lower in the flower organs of 'Porto'. At the molecular level, several differential expressed genes were observed between the cultivars and flower organs. Among the highly expressed DEGs, coat protein, alcohol dehydrogenase, and AP2/EREBP transcription factor ERF-1. Among the downregulated genes, GH3 and NCED were the most interesting. The differential expression of these genes may explain the salt stress tolerance of 'Porto'.

Increased sensitivity of sea urchin larvae to metal toxicity as a consequence of the past two decades of Climate Change and Ocean Acidification in the Mediterranean Sea.

The Mediterranean Sea represents a natural laboratory to infer the possible impacts of climate change and ocean acidification. In this article, we report the deteriorating ability of sea urchin larvae (Paracentrotus lividus) to cope with toxicity of a reference contaminant (Cu EC50) over the past 20 years and assessed the influence of 5 environmental factors from satellite measurements. This timeframe was divided in before and after January 2016 (46.57 µg/L vs 28.56 µg/L respectively, p < 0.001). In the second subset of data, correlation of the biological variable with CO2 and pH strengthened compared to the first part (rCO2-EC50: -0.21 vs -0.83 and rpH-EC50: 0.25 vs 0.87 respectively), with a causal link starting from one year and ending 4 months prior to EC50 measurements. Considering the continuous increase in CO2 concentrations recorded recently, this study could reveal a rapid deterioration of the health condition of this population of sea urchins in a coastal ecosystem.