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Antonie Van Leeuwenhoek ; 64(3-4): 285-305, 1993.
Artigo em Inglês | MEDLINE | ID: mdl-8085791

RESUMO

Comparative sequence analyses were performed on 14 genes encoding bacterial elongation factors EF-Tu and 7 genes encoding the beta-subunit of bacterial F1F0 type ATP-synthases. The corresponding predicted amino acid sequences were compared with published primary structures of homologous molecules. Phylogenetic trees were reconstructed from both data sets of aligned protein sequences and from an equivalent selection of 16S rRNA sequences by applying distance matrix and maximum parsimony methods. The EF-Tu data were in very good agreement with the rRNA data, although the resolution within the EF-Tu tree was reduced at certain phylogenetic levels. The resolution power of the ATPase beta-subunit sequence data were more reduced than those of the EF-Tu data. In comparison with the 16S rRNA tree there are minor differences in the order of adjacent branchings within the ATPase beta-subunit tree.


Assuntos
Bactérias/genética , Bactérias/metabolismo , Genes Bacterianos , Fator Tu de Elongação de Peptídeos/genética , Filogenia , ATPases Translocadoras de Prótons/genética , Sequência de Aminoácidos , Sequência de Bases , Primers do DNA , Substâncias Macromoleculares , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Mapeamento por Restrição , Homologia de Sequência de Aminoácidos
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