RESUMO
Olfactory-mediated behaviors in fish are often examined in artificial microcosms that enable well-controlled treatments but fail to replicate environmental and social contexts. However, observing these behaviors in nature poses challenges. Here, we describe a protocol for recording sea lamprey (Petromyzon marinus) behaviors in a natural system. We describe steps for administering and verifying accurate odorant concentrations, surveying sea lamprey abundance, and tracking sea lamprey movements. We also detail procedures to analyze treatment effects on pheromone-mediated spawning in a high-density population. For complete details on the use and execution of this protocol, please refer to Scott et al.1.
Assuntos
Petromyzon , Feromônios , Animais , Feromônios/farmacologia , Petromyzon/fisiologiaRESUMO
The sensory trap model of signal evolution suggests that males manipulate females into mating using traits that mimic cues used in a nonsexual context. Despite much empirical support for sensory traps, little is known about how females evolve in response to these deceptive signals. Female sea lamprey (Petromyzon marinus) evolved to discriminate a male sex pheromone from the larval odor it mimics and orient only toward males during mate search. Larvae and males release the attractant 3-keto petromyzonol sulfate (3kPZS), but spawning females avoid larval odor using the pheromone antagonist, petromyzonol sulfate (PZS), which larvae but not males, release at higher rates than 3kPZS. We tested the hypothesis that migratory females also discriminate between larval odor and the male pheromone and orient only to larval odor during anadromous migration, when they navigate within spawning streams using larval odor before they begin mate search. In-stream behavioral assays revealed that, unlike spawning females, migratory females do not discriminate between mixtures of 3kPZS and PZS applied at ratios typical of larval versus male odorants. Our results indicate females discriminate between the sexual and nonsexual sources of 3kPZS during but not outside of mating and show sensory traps can lead to reliable sexual communication without females shifting their responses in the original context.
RESUMO
Sex pheromones impart maximal attraction when their components are present at optimal ratios that confer balanced olfactory inputs in potential mates. Altering ratios or adding pheromone analogs to optimal mixtures may disrupt balanced olfactory antagonism and result in reduced attraction, however, tests in natural populations are lacking. We tested this hypothesis in sea lamprey (Petromyzon marinus), a fish whose male sex pheromone attracts females when two critical components, 3-keto petromyzonol sulfate (3kPZS) and petromyzonol sulfate (PZS), are present at certain ratios. Here, we report a pheromone analog, petromyzonol tetrasulfate (3sPZS), reduced female attraction to 3kPZS but not to PZS. 3sPZS mixed with additional PZS synergistically disrupted female attraction to the male pheromone and reduced spawning by 97% in a high-density population. Our results provide evidence of balanced olfactory antagonism in a vertebrate and establish a tactic to disrupt spawning of sea lamprey, a destructive invader of the Laurentian Great Lakes.
RESUMO
The detection of predation risk without direct engagement with a predator is an important driver of prey movement strategies. Consequently, the application of alarm cues may prove an effective tool in guiding the movements of fishes targeted for control or conservation. However, failure to contemplate the sensory, physiological and cognitive outcomes of repeated or persistent exposure to the cue will likely lead to poor performance of management practices. Using a series of behavioural tests and physiological recordings from the olfactory organ, we examined the timing of onset and recovery of the alarm response in sea lamprey (Petromyzon marinus L.) when exposed continuously or sporadically to its alarm cue. In the laboratory, sea lamprey exhibited short-term, reversible attenuation of the alarm response over 2-4 h with continuous exposure. The alarm response spontaneously recovered after 30-60 min of removal from the cue. In long-duration free-swimming tests, where the animals were allowed to move into and out of the odour plume volitionally, repeated but sporadic encounter with the alarm cue over 5 h did not alter the alarm response. Electro-olfactogram recordings from the main olfactory epithelium indicated that olfactory sensory neurons quickly adapt to alarm cue and recovered within 15 min. Our findings strongly implicate habituation as the mechanism that induces reduction in the alarm response and provide insight into the design of effective management practices that seek to use fish alarm cues as repellents.
RESUMO
Biogenic amines activate G-protein-coupled receptors (GPCRs) in the central nervous system in vertebrate animals. Several biogenic amines, when excreted, stimulate trace amine-associated receptors (TAARs), a group of GPCRs in the main olfactory epithelium, and elicit innate behaviors. How TAARs recognize amines with varying numbers of amino groups is largely unknown. We reasoned that a comparison between lamprey and mammalian olfactory TAARs, which are thought to have evolved independently but show convergent responses to polyamines, may reveal structural determinants of amine recognition. Here, we demonstrate that sea lamprey TAAR365 (sTAAR365) responds strongly to biogenic polyamines cadaverine, putrescine, and spermine, and shares a similar response profile as a mammalian TAAR (mTAAR9). Docking and site-directed mutagenesis analyses show that both sTAAR365 and mTAAR9 recognize the two amino groups of cadaverine with the conserved Asp3.32 and Tyr6.51 residues. sTAAR365, which has remarkable sensitivity for cadaverine (EC50 = 4 nM), uses an extra residue, Thr7.42, to stabilize ligand binding. These cadaverine recognition sites also interact with amines with four and three amino groups (spermine and spermidine, respectively). Glu7.36 of sTAAR365 cooperates with Asp3.32 and Thr7.42 to recognize spermine, whereas mTAAR9 recognizes spermidine through an additional aromatic residue, Tyr7.43. These results suggest a conserved mechanism whereby independently evolved TAAR receptors recognize amines with two, three, or four amino groups using the same recognition sites, at which sTAAR365 and mTAAR9 evolved distinct motifs. These motifs interact directly with the amino groups of the polyamines, a class of potent and ecologically important odorants, mediating olfactory signaling.
Assuntos
Poliaminas Biogênicas/química , Proteínas de Peixes/química , Simulação de Acoplamento Molecular , Receptores Odorantes/química , Motivos de Aminoácidos , Animais , Sítios de Ligação , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Células HEK293 , Humanos , Lampreias , Camundongos , Mutagênese Sítio-Dirigida , Receptores Odorantes/genética , Receptores Odorantes/metabolismoRESUMO
Pheromonal bile salts are important for sea lampreys (Petromyzon marinus Linnaeus) to complete their life cycle. The synthesis and release of a releaser/primer pheromone 3-keto petromyzonol sulfate (3kPZS) by spermiating males have been well characterized. 3kPZS evokes sexual behaviors in ovulatory females, induces immediate 3kPZS release in spermiating males, and elicits neuroendocrine responses in prespawning adults. Another primer pheromone released by spermiating males, 3-keto allocholic acid (3kACA), antagonizes the neuroendocrine effects of 3kPZS in prespermiating males. However, the effects of 3kACA and 3kPZS on pheromone production in prespawning adults is unclear. To understand the foundation of pheromone production, we examined sea lamprey bile salt levels at different life stages. To investigate the priming effects of 3kACA and 3kPZS, we exposed prespawning adults with vehicle or synthetic 3kACA or 3kPZS. We hypothesized that endogenous bile salt levels were life-stage and sex-dependent, and differentially affected by 3kACA and 3kPZS in prespawning adults. Using ultra-performance liquid chromatography tandem mass spectrometry, we found that sea lampreys contained distinct mixtures of bile salts in the liver and plasma at different life stages. Males usually contained higher amounts of bile salts than females. Petromyzonamine disulfate was the most abundant C27 bile salt and petromyzonol sulfate was the most abundant C24 bile salt. Waterborne 3kACA and 3kPZS exerted differential effects on bile salt production in the liver and gill, their circulation and clearance in the plasma, and their release into water. We conclude that bile salt levels are life-stage and sex-dependent and differentially affected by primer pheromones.
RESUMO
The evolution of male signals and female preferences remains a central question in the study of animal communication. The sensory trap model suggests males evolve signals that mimic cues used in nonsexual contexts and thus manipulate female behavior to generate mating opportunities. Much evidence supports the sensory trap model, but how females glean reliable information from both mimetic signals and their model cues remains unknown. We discovered a mechanism whereby a manipulative male signal guides reliable communication in sea lamprey (Petromyzon marinus). Migratory sea lamprey follow a larval cue into spawning streams; once sexually mature, males release a pheromone that mimics the larval cue and attracts females. Females conceivably benefit from the mimetic pheromone during mate search but must discriminate against the model cue to avoid orienting toward larvae in nearby nursery habitats. We tested the hypothesis that spawning females respond to petromyzonol sulfate (PZS) as a behavioral antagonist to avoid attraction to the larval cue while tracking the male pheromone despite each containing attractive 3-keto petromyzonol sulfate (3kPZS). We found 1) PZS inhibited electrophysiological responses to 3kPZS and abated preferences for 3kPZS when mixed at the same or greater concentrations, 2) larvae released more PZS than 3kPZS whereas males released more 3kPZS than PZS, and 3) mixtures of 3kPZS and PZS applied at ratios measured in larval and male odorants resulted in the discrimination observed between the natural odors. Our study elucidates how communication systems that arise via deception can facilitate reliable communication.
Assuntos
Lampreias/fisiologia , Feromônios/antagonistas & inibidores , Feromônios/fisiologia , Comunicação Animal , Animais , Mimetismo Biológico/fisiologia , Ácidos Cólicos/química , Ácidos Cólicos/metabolismo , Ecossistema , Feminino , Lampreias/metabolismo , Larva , Masculino , Petromyzon/metabolismo , Petromyzon/fisiologia , Atrativos Sexuais/metabolismo , Atrativos Sexuais/farmacologiaRESUMO
Semen is fundamental for sexual reproduction. The non-sperm part of ejaculated semen, or seminal plasma, facilitates the delivery of sperm to the eggs. The seminal plasma of some species with internal fertilization contains anti-aphrodisiac molecules that deter promiscuity in post-copulatory females, conferring fitness benefits to the ejaculating male. By contrast, in some taxa with external fertilization such as fish, exposure to semen promotes spawning behaviors. However, no specific compounds in semen have been identified as aphrodisiac pheromones. We sought to identify a pheromone from the milt (fish semen) of sea lamprey (Petromyzon marinus), a jawless fish that spawns in lek-like aggregations in which each spermiating male defends a nest, and ovulatory females move from nest to nest to mate. We postulated that milt compounds signal to ovulatory females the presence of spawning spermiating males. We determined that spermine, an odorous polyamine initially identified from human semen, is indeed a milt pheromone. At concentrations as low as 10-14 molar, spermine stimulated the lamprey olfactory system and attracted ovulatory females but did not attract males or pre-ovulatory females. We found spermine activated a trace amine-associated receptor (TAAR)-like receptor in the lamprey olfactory epithelium. A novel antagonist to that receptor nullified the attraction of ovulatory females to spermine. Our results elucidate a mechanism whereby a seminal plasma pheromone attracts ready-to-mate females and implicates a possible conservation of the olfactory detection of semen from jawless vertebrates to humans. Milt pheromones may also have management implications for sea lamprey populations.
Assuntos
Petromyzon/fisiologia , Feromônios/metabolismo , Sêmen/metabolismo , Atrativos Sexuais/metabolismo , Espermatozoides/fisiologia , Espermina/metabolismo , Animais , Feminino , Células HEK293 , Humanos , Masculino , Petromyzon/metabolismo , Reprodução/fisiologia , Espermatozoides/metabolismoRESUMO
Three novel bile acid derivatives, petromylidenes Aâ»C (1â»3), featuring uncommon alkylidene adductive scaffolds, were isolated from water conditioned with sexually mature male sea lampreys (Petromyzon marinus). Their structures were elucidated by mass spectrometry and NMR spectroscopy, and by comparison to spectral data of related structures. The identification of compounds 1â»3, further illustrates the structural diversity of the 5α bile salt family. Compounds 1â»3 exhibited notable biological properties as well, including high olfactory potencies in adult sea lampreys and strong behavioral attraction of ovulated female sea lampreys. Electro-olfactogram recordings indicated that the limit of detection for 1 was 10-9 M, 2 was 10-11 M, and 3 was less than 10-13 M. These results suggested 1â»3 were likely male pheromones, which guide reproductive behaviors in the sea lamprey.
Assuntos
Ácidos e Sais Biliares/farmacologia , Mucosa Olfatória/efeitos dos fármacos , Petromyzon , Atrativos Sexuais/farmacologia , Comportamento Sexual Animal/efeitos dos fármacos , Animais , Ácidos e Sais Biliares/química , Ácidos e Sais Biliares/isolamento & purificação , Feminino , Limite de Detecção , Espectroscopia de Ressonância Magnética , Masculino , Espectrometria de Massas , Estrutura Molecular , Atrativos Sexuais/química , Atrativos Sexuais/isolamento & purificaçãoRESUMO
Bioassay-guided fractionation is an iterative approach that uses the results of physiological and behavioral bioassays to guide the isolation and identification of an active pheromone compound. This method has resulted in the successful characterization of the chemical signals that function as pheromones in a wide range of animal species. Sea lampreys rely on olfaction to detect pheromones that mediate behavioral or physiological responses. We use this knowledge of fish biology to posit functions of putative pheromones and to guide the isolation and identification of active pheromone components. Chromatography is used to extract, concentrate, and separate compounds from the conditioned water. Electro-olfactogram (EOG) recordings are conducted to determine which fractions elicit olfactory responses. Two-choice maze behavioral assays are then used to determine if any of the odorous fractions are also behaviorally active and induce a preference. Spectrometric and spectroscopic methods provide the molecular weight and structural information to assist with the structure elucidation. The bioactivity of the pure compounds is confirmed with EOG and behavioral assays. The behavioral responses observed in the maze should ultimately be validated in a field setting to confirm their function in a natural stream setting. These bioassays play a dual role to 1) guide the fractionation process and 2) confirm and further define the bioactivity of isolated components. Here, we report the representative results of a sea lamprey pheromone identification that exemplify the utility of the bioassay-guided fractionation approach. The identification of sea lamprey pheromones is particularly important because a modulation of its pheromone communication system is among the options considered to control the invasive sea lamprey in the Laurentian Great Lakes. This method can be readily adapted to characterize the chemical communication in a broad array of taxa and shed light on waterborne chemical ecology.
Assuntos
Bioensaio/métodos , Fracionamento Químico/métodos , Petromyzon/crescimento & desenvolvimento , Feromônios/química , AnimaisRESUMO
Olfactory cues provide critical information for spatial orientation of fish, especially in the context of anadromous migrations. Born in freshwater, juveniles of anadromous fish descend to the ocean where they grow into adults before migrating back into freshwater to spawn. The reproductive migrants, therefore, are under selective pressures to locate streams optimal for offspring survival. Many anadromous fish use olfactory cues to orient toward suitable streams. However, no behaviorally active compounds have been identified as migratory cues. Extensive studies have shown that the migratory adult sea lampreys (Petromyzon marinus), a jawless fish, track a pheromone emitted by their stream-dwelling larvae, and, consequently, enter streams with abundant larvae. We fractionated extracts of larval sea lamprey washings with guidance from a bioassay that measures in-stream migratory behaviors of adults and identified four dihydroxylated tetrahydrofuran fatty acids, of which (+)-(2S,3S,5R)-tetrahydro-3-hydroxy-5-[(1R)-1-hydroxyhexyl]-2-furanoctanoic acid was shown as a migratory pheromone. The chemical structure was elucidated by spectroscopies and confirmed by chemical synthesis and X-ray crystallography. The four fatty acids were isomer-specific and enantiomer-specific in their olfactory and behavioral activities. A synthetic copy of the identified pheromone was a potent stimulant of the adult olfactory epithelium, and, at 5 × 10-13 M, replicated the extracts of larval washings in biasing adults into a tributary stream. Our results reveal a pheromone that bridges two distinct life stages and guides orientation over a large space that spans two different habitats. The identified molecule may be useful for control of the sea lamprey.
Assuntos
Migração Animal/efeitos dos fármacos , Ácidos Graxos , Lampreias/fisiologia , Feromônios , Migração Animal/fisiologia , Animais , Ácidos Graxos/química , Ácidos Graxos/farmacologia , Feromônios/química , Feromônios/farmacologiaRESUMO
Ligand-based virtual screening has become a standard technique for the efficient discovery of bioactive small molecules. Following assays to determine the activity of compounds selected by virtual screening, or other approaches in which dozens to thousands of molecules have been tested, machine learning techniques make it straightforward to discover the patterns of chemical groups that correlate with the desired biological activity. Defining the chemical features that generate activity can be used to guide the selection of molecules for subsequent rounds of screening and assaying, as well as help design new, more active molecules for organic synthesis.The quantitative structure-activity relationship machine learning protocols we describe here, using decision trees, random forests, and sequential feature selection, take as input the chemical structure of a single, known active small molecule (e.g., an inhibitor, agonist, or substrate) for comparison with the structure of each tested molecule. Knowledge of the atomic structure of the protein target and its interactions with the active compound are not required. These protocols can be modified and applied to any data set that consists of a series of measured structural, chemical, or other features for each tested molecule, along with the experimentally measured value of the response variable you would like to predict or optimize for your project, for instance, inhibitory activity in a biological assay or ΔGbinding. To illustrate the use of different machine learning algorithms, we step through the analysis of a dataset of inhibitor candidates from virtual screening that were tested recently for their ability to inhibit GPCR-mediated signaling in a vertebrate.
Assuntos
Biologia Computacional/métodos , Receptores Acoplados a Proteínas G/química , Receptores Acoplados a Proteínas G/metabolismo , Bibliotecas de Moléculas Pequenas/química , Animais , Avaliação Pré-Clínica de Medicamentos , Ensaios de Triagem em Larga Escala , Humanos , Ligantes , Aprendizado de Máquina , Ligação Proteica , Relação Quantitativa Estrutura-Atividade , Transdução de Sinais/efeitos dos fármacos , Bibliotecas de Moléculas Pequenas/farmacologia , Vertebrados/metabolismoRESUMO
While the advantage of screening vast databases of molecules to cover greater molecular diversity is often mentioned, in reality, only a few studies have been published demonstrating inhibitor discovery by screening more than a million compounds for features that mimic a known three-dimensional (3D) ligand. Two factors contribute: the general difficulty of discovering potent inhibitors, and the lack of free, user-friendly software to incorporate project-specific knowledge and user hypotheses into 3D ligand-based screening. The Screenlamp modular toolkit presented here was developed with these needs in mind. We show Screenlamp's ability to screen more than 12 million commercially available molecules and identify potent in vivo inhibitors of a G protein-coupled bile acid receptor within the first year of a discovery project. This pheromone receptor governs sea lamprey reproductive behavior, and to our knowledge, this project is the first to establish the efficacy of computational screening in discovering lead compounds for aquatic invasive species control. Significant enhancement in activity came from selecting compounds based on one of the hypotheses: that matching two distal oxygen groups in the 3D structure of the pheromone is crucial for activity. Six of the 15 most active compounds met these criteria. A second hypothesis-that presence of an alkyl sulfate side chain results in high activity-identified another 6 compounds in the top 10, demonstrating the significant benefits of hypothesis-driven screening.
Assuntos
Bases de Dados de Compostos Químicos , Descoberta de Drogas , Receptores Acoplados a Proteínas G/antagonistas & inibidores , Animais , Ácidos e Sais Biliares/química , Avaliação Pré-Clínica de Medicamentos/métodos , Espécies Introduzidas , Ligantes , Petromyzon , Ligação Proteica , Bibliotecas de Moléculas Pequenas/química , SoftwareRESUMO
Two novel sulfated bile salt-like dienones, featuring either a unique, rearranged side chain or a rare cis-11,12-diol on the steroidal C-ring, herein named petromyzene A (1) and B (2), respectively, were isolated from water conditioned with spawning male sea lamprey (Petromyzon marinus; a jawless vertebrate animal). The structures of these natural products were elucidated by mass spectrometry and NMR spectroscopy. Petromyzenes A and B exhibited high olfactory potency for adult sea lamprey and strong behavioral attraction for spawning females.
Assuntos
Ácidos e Sais Biliares/química , Animais , Feminino , Masculino , Estrutura Molecular , Petromyzon , EsteroidesRESUMO
Sea lamprey, Petromyzon marinus, rely heavily on chemical cues that mediate their life history events, such as migration and reproduction. Here, we describe petromyzone A-C (1-3), three novel bile alcohols that are highly oxidized and sulfated, isolated from water conditioned with spermiated male sea lamprey. Structures of these compounds were unequivocally established by spectroscopic analyses and by comparison with spectra of known compounds. Electro-olfactogram recordings showed that 1 at 10-11 M was stimulatory to the adult sea lamprey olfactory epithelium, while 2 and 3 were stimulatory at 10-13 M. Behavioral assays indicated that 1 is attractive, 2 is not attractive or repulsive, and 3 is repulsive to ovulated female sea lamprey. The results suggest that 1 and 2 may be putative pheromones that mediate chemical communication in sea lamprey. The identification of these three components enhances our understanding of the structures and functions of sex pheromone components in this species and may provide useful behavioral manipulation tools for the integrated management of sea lamprey, a destructive invader in the Laurentian Great Lakes.
Assuntos
Colestanóis/química , Colestanóis/metabolismo , Petromyzon/fisiologia , Atrativos Sexuais/química , Atrativos Sexuais/fisiologia , Animais , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Sinais (Psicologia) , Feminino , Masculino , Espectrometria de Massas , Estrutura Molecular , Mucosa Olfatória/fisiologia , Ovulação , Esteroides/química , Esteroides/metabolismoRESUMO
A sensitive and reliable method was developed and validated for the determination of unsaturated bile alcohols in sea lamprey tissues using liquid-liquid extraction and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The liver, kidney, and intestine samples were extracted with acetonitrile and defatted by n-hexane. Gradient UHPLC separation was performed using an Acquity BEH C18 column with a mobile phase of water and methanol containing 20 mM triethylamine. Multiple reaction monitoring modes of precursor-product ion transitions for each analyte was used. This method displayed good linearity, with correlation coefficients greater than 0.99, and was validated. Precision and accuracy (RSD %) were in the range of 0.31%-5.28%, while mean recoveries were between 84.3%-96.3%. With this technique, sea lamprey tissue samples were analyzed for unsaturated bile alcohol analytes. This method is practical and particularly suitable for widespread putative pheromone residue analysis.
Assuntos
Colestanóis/química , Colestanóis/metabolismo , Lampreias/metabolismo , Animais , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas , OxirreduçãoRESUMO
Total knee replacement (total knee arthroplasty) is performed to restore function and relieve pain in patients with severely damaged knees. The surgery involves replacement of both the medial and lateral femorotibial joints and the patellofemoral joint. Although total knee replacement is an effective treatment, postoperative complications include blood clots, infection, and loosening or malalignment of the prosthetic component. Medical imaging plays a critical role in preoperative evaluation, surgical planning, and followup.
Assuntos
Artrografia/métodos , Artroplastia do Joelho/métodos , Articulação do Joelho/diagnóstico por imagem , Articulação do Joelho/cirurgia , Osteoartrite do Joelho/diagnóstico por imagem , Osteoartrite do Joelho/cirurgia , Artroplastia do Joelho/instrumentação , Humanos , Seleção de Pacientes , Cuidados Pré-Operatórios/métodos , Cirurgia Assistida por Computador/métodos , Resultado do TratamentoRESUMO
Mature male sea lampreys (Petromyzon marinus) release a sex pheromone, 3-keto-petromyzonol sulfate (3kPZS), that induces sexually dimorphic behavioral responses in conspecifics. However, the neural mechanism of such responses is mostly unknown. We examined the neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) and the expression of 5-HT1A receptors in the forebrain and brainstem of sea lamprey exposed to the vehicle (0.91 ppm methanol) or 10(-10) M 3kPZS for 2 h using high performance liquid chromatography-electrospray ionization tandem mass spectrometry, immunohistochemistry and real-time quantitative polymerase chain reaction. Exposure to 3kPZS for 2 h increased 5-HT concentration in the forebrain of adult females, whereas 5-HT was not detected in the forebrain of adult males. On the contrary, 3kPZS exposure decreased 5-HT concentration in the brainstem of adult females and had no effect in adult males. Pheromone exposure evoked context-dependent sexual dimorphism in brain 5-HT1A receptor immunoreactivity, but had no effect on 5-HT1A mRNA concentrations in the brain with 2 h exposure time. It appears that in sea lamprey pheromone 3kPZS affects the 5-HT system in the brain in a context-dependent, sexually dimorphic manner.
Assuntos
Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Ácidos Cólicos/farmacologia , Petromyzon/fisiologia , Serotonina/metabolismo , Animais , Tronco Encefálico/efeitos dos fármacos , Tronco Encefálico/metabolismo , Feminino , Regulação da Expressão Gênica , Masculino , Feromônios/farmacologia , Reação em Cadeia da Polimerase em Tempo Real , Receptores 5-HT1 de Serotonina , Caracteres Sexuais , Espectrometria de Massas em TandemRESUMO
Biliary atresia is a rare disease of infancy, with an estimated 1 in 15,000 frequency in the southeast United States, but more common in East Asian countries, with a reported frequency of 1 in 5,000 in Taiwan. Although much is known about the management of biliary atresia, its pathogenesis is still elusive. The sea lamprey (Petromyzon marinus) provides a unique opportunity to examine the mechanism and progression of biliary degeneration. Sea lamprey develop through three distinct life stages: larval, parasitic, and adult. During the transition from larvae to parasitic juvenile, sea lamprey undergo metamorphosis with dramatic reorganization and remodeling in external morphology and internal organs. In the liver, the entire biliary system is lost, including the gall bladder and the biliary tree. A newly-developed method called "CLARITY" was modified to clarify the entire liver and the junction with the intestine in metamorphic sea lamprey. The process of biliary degeneration was visualized and discerned during sea lamprey metamorphosis by using laser scanning confocal microscopy. This method provides a powerful tool to study biliary atresia in a unique animal model.
Assuntos
Sistema Biliar/ultraestrutura , Fígado/crescimento & desenvolvimento , Microscopia Confocal/métodos , Petromyzon/crescimento & desenvolvimento , Animais , Atresia Biliar/patologia , Modelos Animais de Doenças , Metamorfose BiológicaRESUMO
Minimizing radiation exposure while optimizing image quality is critical in diagnostic medical imaging. Radiologic technologists face complex challenges when implementing dose monitoring and reporting systems to assist in radiation safety efforts. Challenges include communicating the risks of radiation exposure, handling variations in dose measurement techniques, and working with a lack of standardized nomenclature for examination types. With increasing attention on radiation safety, many solutions have been presented. A team approach to dose optimization with professionals nationwide can help establish a culture of radiation safety in medical imaging.