Effect of supplemental vitamin D3 on bone mineral density: a systematic review and meta-analysis.

CONTEXT: There is still controversy over the effect of vitamin D3 supplementation on bone health. OBJECTIVE: The effects of vitamin D3 supplementation on bone mineral density (BMD) and markers of bone turnover, as well as the dose-response relationship between vitamin D3 and bone health in adults, were evaluated. DATA SOURCES: The PubMed, Scopus, Cochrane, Web of Science, and AGRIS databases were searched for articles published through April 30, 2022. Thirty-nine of the 6409 records identified met the inclusion criteria. DATA EXTRACTION: Data were extracted from articles by 2 authors, and data extraction was cross-checked independently. A random-effects model was used to estimate the pooled effect size and the associated 95%CI for the effect of vitamin D3 for each outcome. A one-stage random-effects dose-response model was used to estimate the dose-response relationship between vitamin D3 supplementation and BMD. DATA ANALYSIS: Results of meta-analysis showed a beneficial effect of vitamin D3 at the lumbar spine (standardized mean difference [SMD] = 0.06; 95%CI, 0.01-0.12) and femoral neck (SMD = 0.25; 95%CI, 0.09-0.41). Dose-response analysis revealed a linear relationship between vitamin D3 supplementation doses and BMD at the femoral neck, lumbar spine, and total hip sites. No significant effect of vitamin D3 supplementation on whole-body or total hip BMD was observed (P > 0.05). Vitamin D3 supplementation significantly decreased BMD at both proximal and distal forearm (SMD = -0.16; 95%CI, -0.26 to -0.06). The variables of ethnicity, age, baseline 25-hydroxyvitamin D (25[OH]D), menopause status, vitamin D3 dosing frequency, and bone health status (P interaction = 0.02) altered the effect of vitamin D3 supplementation on BMD. Additionally, a nonlinear relationship between vitamin D3 supplement doses and markers of bone turnover was found. CONCLUSION: A protective effect of vitamin D3 supplementation on BMD of the lumbar spine, femoral neck, and total hip is implicated. SYSTEMATIC REVIEW REGISTRATION: PROSPERO registration number CRD42017054132.

Immunity to hepatitis A virus in liver transplant recipients: A population-based study in Iran.

BACKGROUND: Acute hepatitis A is usually a self-limited viral disease but can be severe and even fatal in special groups of patients including those with chronic liver disease and recipients of liver transplantation. To take appropriate preventive measures, it is important to determine the immune status against the hepatitis A virus in patients at risk of grave clinical outcomes following infection. To assess the need for immunization against hepatitis A, we aimed to determine the immune status against hepatitis A in a population of liver transplant recipients. We also investigated the association between hepatitis A immune status and demographic factors such as age and sex, underlying liver disease, source of drinking water, geographical area of residence and socioeconomic status. METHODS: This cross-sectional study was performed on 242 recipients of allogenic liver transplants at Abu Ali Sina Organ Transplant Hospital in Shiraz, Iran, between January 2017 and April 2017. The level of immunity was assessed using hepatitis A antibody detection kits. RESULTS: The rate of immunity against hepatitis A was detected as 88.8% in our study population. In the multivariable logistic regression model, younger age (OR=1.175, P<0.001) and higher education level (OR=2.142, P=0.040) were the main determinants of non-immune status. However, hepatitis A immunity was independent of gender, monthly family income, water supply source, residential area and underlying liver disorder. CONCLUSION: Although a significant proportion of liver transplant recipients in this study showed evidence of natural immunity to hepatitis A, a considerable proportion of younger patients and those with a higher level of education were non-immune. The results of this study signify the importance of screening for hepatitis A immunity in this at-risk population of patients and the need for vaccinating non-immune patients.

Investigation of IL-17A Serum Levels in Patients with Nonmelanoma Skin Cancer.

BACKGROUND: Role of interleukin 17A (IL-17A) in carcinogenesis and cancer growth is controversial. Although some researches support its antitumor activity, some others suggest that it promotes the growth and development of different types of cancer including skin cancer by activation of STAT3. Although the function of the cytokines such as IL-17A has been extensively studied in various types of cancer, nonmelanoma skin cancer (NMSC) has not received much attention. Therefore, the present study was aimed to investigate the serum levels of IL-17A in NMSC patients. METHODS: This cross-sectional study was performed on 60 patients with basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) as well as 57 age-sex matched healthy individuals as control group. Measurement of IL-17A serum levels in both case and control groups was performed by a commercially reliable sandwich enzyme-linked immunosorbent assay (ELISA) kit. RESULTS: In this study, we observed that IL-17A serum levels in NMSC patients were significantly higher than the control group (P < 0.001). Also, both BCC and SCC patients had higher levels of IL-17A in their sera in comparison to the controls (P=0.001 and P < 0.001, respectively). However, there was no significant difference between SCC and BCC patients regarding serum levels of IL-17A. CONCLUSION: According to our results, it can be concluded that IL-17A may play a role in inducing the growth and progression of NMSC and it can be used as a therapeutic target in these patients in future.

Gaussian Graphical Models Identified Food Intake Networks among Iranian Women with and without Breast Cancer: A Case-Control Study.

BACKGROUND: Dietary patterns may be an important predictor of breast cancer risk. However, they cannot completely explain the pairwise correlations among foods. The purpose of this study is to compare food intake networks derived by Gaussian Graphical Models (GGMs) for women with and without breast cancer to better understand how foods are consumed in relation to each other according to disease status. METHODS: A total of 134 women with breast cancer and 267 hospital controls were selected from referral hospitals of Tehran, Iran. Dietary intakes were evaluated by using a validated 168 food-items semi-quantitative food frequency questionnaire. GGMs were applied to log-transformed intakes of 28 food groups to construct outcome-specific food networks. RESULTS: Among cases, a main network containing intakes of 12 central food groups (vegetables, fruits, nuts and seeds, olive oil and olive, processed meat, sweets, salt, soft drinks, fried potatoes, pickles, low-fat dairy, pizza) was detected. In controls, a main network including six central food groups (liquid oils, vegetables, fruits, sweets, fried potatoes and soft drinks) was identified. CONCLUSIONS: The findings of this study revealed a difference in GGM-identified networks graphs between cases and controls. Overall, GGM may provide additional understanding of relationships between diet and health.

Dietary Approaches to Stop Hypertension (DASH) diets and breast cancer among women: a case control study.

BACKGROUND: Studying entire dietary patterns is a promising alternative approach to overcome limitations of the single food or nutrient approach. We evaluated the relationship between the scores of 4 established Dietary Approaches to Stop Hypertension (DASH) diet indexes and breast cancer risk among Iranian women. METHODS: This case-control study was carried out on 408 eligible women (136 cases and 272 hospital-based controls). A validated 168 item semi-quantitative food frequency questionnaire was used for assessing usual dietary intakes. DASH index scores were generated based on predefined algorithms for each of the 4 previously described indexes (Dixon's, Mellen's, Fung's and Günther's DASH diet index). Unconditional logistic regression analysis was performed to estimate odds ratio (OR) and 95% confidence intervals (CIs) for score categories or quintiles of DASH diet indexes and breast cancer risk in multivariate adjusted models. RESULTS: Women in the highest categories of the Mellen's and Günther's scores had lower odds of breast cancer than those in the lowest quintiles (Mellen's OR:0.50; 95% CI:0.62-0.97; P-trend:0.02; Günther's OR:0.48; 95% CI:0.25-0.93; P-trend:0.05). However, no significant associations were found between Dixon's and Fung's DASH score and breast cancer risk. Modification by menopausal status revealed that breast cancer risk was only reduced in postmenopausal women with higher scores on Mellen's index (OR:0.24; 95% CI:0.08-0.68; P-trend:0.04). CONCLUSION: A greater adherence to 2 of the 4 DASH indexes (Mellen's and Günther's indexes) was associated with decreased risk of breast cancer.

The anticancer effects of curcumin via targeting the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway.

The mammalian target of rapamycin (mTOR) is a protein kinase that has been considered as a key regulator of a large number of cellular processes, including cell growth, proliferation, differentiation, survival, and motility. Overactivation of mTOR (especially mTORC1) signaling is related to oncogenic cellular processes. Therefore targeting mTORC1 signaling is a new promising strategy in cancer therapy. In this regard, various studies have shown that curcumin, a polyphenol produced from the turmeric rhizome, has anti-inflammatory, antioxidant and anticancer properties. Curcumin may exert its anticancer function, at least in part, by suppressing mTOR-mediated signaling pathway in tumor cells. However, the exact underlying mechanisms by which curcumin blocks the mTORC1 signaling remain unclear. According to literature, curcumin inhibits insulin-like growth factor 1 (IGF-1)/phosphoinositide 3-kinase (PI3K)/Akt/mTORC1 pathway which leads to apoptosis and cell cycle arrest via suppression of erythroblastosis virus transcription factor 2 and murine double minute 2 oncoprotein. In addition, activation of unc-51-like kinase 1 by curcumin, as a downstream target of IGF-1/PI3K/Akt/mTORC1 axis, enhances autophagy. Curcumin induces AMP-activated protein kinase, a negative regulator of mTORC1, via inhibition of F0F1-ATPase. Interestingly, curcumin suppresses IκB kinase ß, the upstream kinase in mTORC1 pathway. Moreover, evidence revealed that curcumin downregulates the E3-ubiquitin ligases NEDD4, neural precursor cell-expressed developmentally downregulated 4. NEDD4 is frequently overexpressed in a wide range of cancers and degrades the phosphatase and tensin homolog, which is a negative regulator of mTORC1. Finally another suggested mechanism is suppression of MAOA/mTORC1/hypoxia-inducible factor 1α signaling pathway by curcumin.

Is the hedonic hunger score associated with obesity in women? A brief communication.

OBJECTIVE: Food intake for its hedonic properties can result in excess caloric intake. It may play a role in increasing trend of obesity in the world. Hedonic hunger may effect on dietary patterns. We assessed the association between dietary patterns and the hedonic score in obese and non-obese women. In this case-control study 140 women aged 17-44 years from an obesity club at district 4 of Tehran participated. Dietary patterns were assessed through food frequency questionnaire by factor analysis method. The hedonic score was determined using a 21-item valid questionnaire. ANOVA and Logistic regression were used to statistical analysis. RESULTS: Factor analysis method showed that there were 2 dietary patterns named healthy and unhealthy, in order to their food items. There was not any significant trend for obesity among tertiles of healthy and unhealthy dietary patterns. The mean hedonic score was significantly higher in obese than non-obese women, (p < 0.05). The second and the third tertiles of hedonic score significantly increase the odds of obesity referring the first tertile (2.8 and 10.8, respectively). Hedonic hunger had a positive association with obesity; but there was no difference in dietary patterns of obese and non-obese women, unexpectedly.

Hypomethylation of NANOG promoter in colonic mucosal cells of obese patients: a possible role of NF-κB.

Obesity and particularly central obesity are the main risk factors of colon cancer. All intestinal cell populations including stem cells, their progenitors and differentiated colonocytes seem to be the origin of colorectal cancer. However, recent data support the role of differentiated cells as cancer origin especially during inflammation. Based on Yamanaka's seminal work, re-expression of few transcription factors in terminally differentiated cells creates stemness properti'es. Although these transcription factors are involved in tumorigenesis, they are epigenetically repressed in adult tissues. We proposed that obesity might regulate methylation of stemness genes in colonocytes via inflammatory signalling. Obesity-associated inflammation was analysed using Western blot analysis of phospho-IκB (inhibitor of NF-κB). Methylation-sensitive high-resolution melting analysis was performed on colonic mucosal samples of twenty obese and twenty normal-weight men to analyse promoter methylation of POU5F1 (OCT4), NANOG, MYC and CDKN2A. TNF-treated HT-29 cells were used to recapitulate the effect of NF-κB activation on stemness genes methylation. Our results showed that colonic phosphorylation of IκB, as a signal of NF-κB activation, was higher in obese subjects compared with their normal-weight counterparts. Moreover, promoter methylation of NANOG was likely to be lower in obese subjects and correlated with central obesity. HT-29 cells incubated by TNF-α showed hypomethylation of POU5F1 and MYC genes in addition to the NANOG. These results suggest that obesity-induced inflammation might be involved in the regulation of DNA methylation of oncogenic genes such as NANOG in differentiated colonocytes and thus predispose them to later oncogenic alterations.

Healthy Eating Index 2010 and Breast Cancer Risk.

BACKGROUND: The role of dietary quality indexes for predicting breast cancer (BrCa) risk remains inconclusive. We aimed to assess the relation between healthy eating index 2010 (HEI-2010) and BrCa risk in a case-control study in Iranian women. METHOD: The 272 hospitalized controls and 136 newly diagnosed BrCa cases were recruited from two major hospitals. Food frequency questionnaire (FFQ) data were then utilized to calculate the HEI-2010 scores. Logistic regression models were used to calculate odds ratios (OR) and 95% confidence intervals (CIs) between tertiles of HEI-2010 and BrCa risk, adjusting for potential confounders. RESULTS: We found that higher HEI-2010 scores were associated with lower risk of BrCa only among premenopausal women (multivariate adjusted OR= 0.27, 95% [CI]: 0.10-0.69; P for trend =0.02). However, this inverse association was not the results of a link with any specific component of the HEI-2010. Among postmenopausal women, lower intake from empty calories was associated with lower risk of BrCa by 13% (OR =0.87, 95% CI: 0.77-0.99). CONCLUSION: Our findings suggest that adherence to HEI-2010 might be associated with lower risk of BrCa only among premenopausal women.

The Effect of Gluten Free Diet on Components of Metabolic Syndrome: A Randomized Clinical Trial

Background: This study aimed to assess the effects of Gluten free diet (GFD) on components of metabolic syndrome (MES). Materials and Methods: In this randomized clinical trial, 50 subjects diagnosed with MES were randomly divided into two groups (n=25). The first group received a GFD and the second group continued their regular diet. Biochemical markers of MES and blood pressure were measured before and after 8-week intervention. Results: Forty five subjects completed the study. A post-hoc comparison of the groups showed no effects of the GFD and control diet on LDL cholesterol, total cholesterol, fasting insulin, HOMA-IR, systolic and diastolic blood pressure levels. The GFD reduced fasting blood glucose, waist circumference (WC) and serum triglyceride concentration significantly compared with the control diet (p<0.05). Conclusion: Short-term GFD reduced WC and improved glycemic control and Triglyceride level in subjects with the metabolic syndrome.

Dietary patterns and breast cancer risk among Iranian women: A case-control study.

AIM: Breast cancer is the most common cancer among women worldwide. Most previous studies focused on individual nutrients or foods rather than overall dietary patterns. We aimed to assess the association between major dietary patterns and breast cancer risk. METHOD: In a hospital-based case-control study, 134 women diagnosed as breast cancer and 267 controls were recruited from referral hospitals in Tehran, Iran. Dietary intakes were evaluated by using a validated 168-item food frequency questionnaire. Dietary pattern were identified by factor analysis. Odds ratios (OR) were estimated using logistic regression. RESULTS: Two dietary patterns were extracted: healthy and unhealthy. The "Healthy" dietary pattern was loaded for high consumptions of fruits, vegetables, seeds, legume, fish and sea foods, whole grains, liquid oils, olive oils and olive and lack of salt intake. "Unhealthy" dietary pattern was characterized by high factor loadings for sweets, soft drinks, mayonnaise, solid oils, processed meat, fried and boiled potato and salt intake. In multivariate logistic regression analyses, the healthy dietary pattern was not associated with breast cancer risk (OR: 0.83; 95%CI: 0.36, 1.89; P-trend = 0.50), while women in the highest quartile of the unhealthy dietary pattern had a significantly increased breast cancer risk (OR: 2.21; 95%CI: 1.04, 4.690; P-trend = 0.009). When stratified by menopausal status, unhealthy dietary pattern showed inverse association with breast cancer risk only among post-menopausal women (OR: 3.56; 95%CI: 1.16, 10.95; P-trend = 0.008). CONCLUSIONS: Our findings showed that an unhealthy dietary pattern might be associated with higher risk of breast among Iranian women.

Nutrient Patterns and Risk of Breast Cancer among Iranian Women: a Case- Control Study

Objective: To explore the role of nutrient patterns in the etiology of breast cancer (BCa) among Iranian women. Methods: The study included 134 newly diagnosed cases of BCa and 267 hospitalized controls. A validated semi-quantitative food frequency questionnaire (FFQ) was used to assess dietary intake. Nutrient patterns were obtained using principal component analysis using Varimax rotation and logistic regression was performed to estimate breast cancer risk. Results: We identified 4 major nutrient patterns. First was high in consumption of vitamins B1, B2, B3, B5, B6, B9, C, magnesium, iron, carbohydrate, fiber, selenium, zinc, protein, potassium, and calcium. The second nutrient pattern included Vitamins B12, A and cholesterol, while the third featured vitamin D, EPA and DHA. The fourth was characterized by vitamin E, MUFA and saturated fatty acids. After adjusting for age, patterns 1 and 3 were associated with a lower risk of BCa (OR=0.51, 95% CI: 0.33- 0.80, P=0.003, OR=0.64, 95% CI: 0.42- 0.98, P= 0.04 respectively). However, after further adjustment for all confounders in multivariate analysis, the association remained significant only for pattern 1 (OR=0.52, 95% CI: 0.32- 0.82, P=0.006). Conclusion: Adherence to a nutrient pattern rich in vitamin B, minerals and fiber is associated with a lower risk of breast cancer.

Dietary Inflammatory Index and Odds of Colorectal Cancer in a Case- Control Study from Iran

Background: In Iran, colorectal cancer (CRC) is the third and fourth leading cause of cancer incidence among men and women, respectively. Diet and inflammation have been suggested as important risk factors for CRC. We examined the association between dietary inflammatory index (DII) scores and CRC in a case-control study conducted in Iran. Methods: This study included 71 CRC cases and 142 controls hospitalized for acute non-neoplastic diseases. DII scores were computed from dietary intake assessed by a previously validated food frequency questionnaire. Logistic regression models were used to estimate odds ratios (ORs) and 95% confidence intervals (CI) adjusted for age, sex, education, energy intake, exercise, body mass index (BMI), smoking, family history of cancer, and history of aspirin, acetaminophen, and multivitamin use. Results: Subjects with higher DII scores (i.e., indicating a more pro-inflammatory diet) had a higher odds of CRC with the DII being used as both a continuous variable (OR continuous = 2.20, 95% CI: 1.22-3.87) and as a categorical variable (OR tertile 3 vs tertile1 = 2.47, 95%CI: 1.10-5.55). Conclusion: These results indicate that a pro-inflammatory diet is associated with increased odds of CRC in this Iranian population.

Adherence to a Mediterranean dietary pattern and overweight and obesity among female adolescents in Iran.

Background Given the high prevalence of overweight and obesity in Iran, identification of a healthy diet is essential. Objective This study aimed to assess the association between adherence to a Mediterranean diet and overweight and obesity in female adolescents. Methods Two hundred and eighty female adolescents from high schools in Tehran were recruited. General information, physical activity and food frequency questionnaires were completed. Adherence to the Mediterranean diet was evaluated using the Mediterranean Style Dietary Pattern Score (MSDPS). Results Under and over reporters of energy intake were excluded from the analysis (n = 37). Results indicated that prevalence of overweight and obesity was 13.3% and 12.5%, respectively. The mean of MSDPS was low in this population (15.99 ± 5.64). The MSDPS was positively associated with mother's education level and energy intake (p < 0.05). No significant relation was observed between MSDPS and overweight and obesity among adolescents. However, there was a negative significant association between MSDPS and WHR just among students who had educated mothers [high: second median v. low: first median, odds ratio (OR) = 0.36, 95% confidence interval (CI) = 0.12-1.03]. Conclusion Adherence to a Mediterranean dietary pattern is low among adolescent females in Tehran, with no association with overweight and obesity.

Bioconversion of shrimp waste Penaeus merguiensis using lactic acid fermentation: An alternative procedure for chemical extraction of chitin and chitosan.

Chitin extraction from shrimp wastes by biological treatment, using the Pseudomonas aeruginosa was a positive and simple method. In order to look for the optimal conditions, the wastes were incubated at 30°C and 100rpm in different glucose (0%, 10%, 15% and 20%) and inoculation (10%, 15% and 20%) concentrations for 4 and 6days. At the end of fermentation, Protease activity was investigated at different temperatures and temperature 50°C was considered as the optimum. The results obtained also showed a direct relationship between the concentration of different parameters and deproteinization and demineralization rates, so that the optimal conditions were 20% glucose, 20% inoculation and 6days fermentation. These conditions led to 82% demineralization, 92% deproteinization and chitin yield of 47%. Then, chitin was converted to chitosan using microwave, autoclave and traditional methods. The highest yield (87%) was obtained with autoclave method. At the end, the chitin and chitosan were characterized by elemental analysis and FTIR.

Nutrient patterns and risk of cataract: a case-control study.

AIM: To assess the relation between nutrient patterns and cataract risk. METHODS: This is a hospital-based case-control study with 97 cataract patients and 198 matched controls. Dietary consumption was collected through a valid food frequency questionnaire (FFQ). Nutrient patterns were detected by applying factor analysis. Unconditional logistic regression models were used to estimate odds ratio (ORs) and 95%CIs. RESULTS: We extracted 5 main nutrient patterns. Factor 1 included niacin, thiamin, carbohydrates, protein, zinc, vitamin B6 and sodium (sodium pattern). Factor 2 was characterized by oleic acid, monounsaturated fats, polyunsaturated fats, linoleic acid, trans fatty acid, linolenic acid, vitamin E and saturated fats (fatty acid pattern). The third factor represented high intake of vitamin B12, vitamin D, cholesterol and calcium (mixed pattern). The 4th pattern was high in intake of beta and alpha carotene, vitamin A and vitamin C (antioxidant pattern). Finally, the 5th pattern loaded heavily on docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) (omega-3 pattern). In crude and multivariate analysis, the sodium pattern was associated with increased risk of cataract (OR=1.97, 95%CI: 1.09-3.96). The fatty acid pattern elevated the risk of cataract (OR=1.94, 95%CI: 1.1-3.86). Antioxidant pattern was associated with a significant 79% reduced risk (2nd category compared with the 1st). Omega-3 pattern was significantly negatively associated with risk of cataract (P=0.04). CONCLUSION: These findings imply that nutrient patterns reflecting a combined consumption of nutrients might be important in the etiology of cataract. Additional studies with more efficient designs are warranted to confirm our findings.

Maternal Dietary Patterns and Gestational Diabetes Risk: A Case-Control Study.

BACKGROUND: Maternal dietary patterns play an important role in the progress of gestational diabetes mellitus (GDM). The aim of the present study was to explore this association. METHOD: A total of 388 pregnant women (122 case and 266 control) were included. Dietary intake were collected using a food frequency questionnaire (FFQ). GDM was diagnosed using a 100-gram, 3-hour oral glucose tolerance test. Dietary pattern was identified by factor analysis. To investigate the relation between each of the independent variables with gestational diabetes, the odds ratio (OR) was calculated. RESULTS: Western dietary pattern was high in sweets, jams, mayonnaise, soft drinks, salty snacks, solid fat, high-fat dairy products, potatoes, organ meat, eggs, red meat, processed foods, tea, and coffee. The prudent dietary pattern was characterized by higher intake of liquid oils, legumes, nuts and seeds, fruits and dried fruits, fish and poultry whole, and refined grains. Western dietary pattern was associated with increased risk of gestational diabetes mellitus before and after adjustment for confounders (OR = 1.97, 95% CI: 1.27-3.04, OR = 1.68, 95% CI: 1.04-2.27). However, no significant association was found for a prudent pattern. CONCLUSION: These findings suggest that the Western dietary pattern was associated with an increased risk of GDM.

Mediterranean diet adherence and risk of multiple sclerosis: a case-control study.

BACKGROUND AND OBJECTIVES: We conducted a hospital-based, case-control study to examine the association between Mediterranean diet (MD) and the risk of multiple sclerosis (MS) in Iran. METHODS AND STUDY DESIGN: A total of 70 patients with MS and 142 controls underwent face-to-face interviews in the major neurological clinics of Tehran, Iran. Adherence to a MD was assessed using the 9-unit dietary score, to evaluate the level of conformity of the individual's diet to the Mediterranean dietary pattern. Multivariate logistic regression was used to estimate odds ratios (OR) and 95% confidence intervals (CI). RESULTS: Higher consumption of fruits (OR=0.28, 95% CI: 0.12-0.63, p-value: 0.002) and vegetables (OR=0.23, 95% CI: 0.10-0.53, p-value: 0.001) were significantly associated with reduced MS risk. In both age adjusted and multivariate adjusted model, the OR of MS decreased significantly in the third as compared to the first tertile of MD score (age adjusted OR: 0.21, 95% CI: 0.06-0.67; p-trend: 0.01, Multivariate adjusted OR: 0.23, 95% CI: 0.06-0.89, p-trend: 0.04). CONCLUSIONS: Our study suggests that a high quality diet assessed by MD may decrease the risk of MS.

Chitin from Penaeus merguiensis via microbial fermentation processing and antioxidant activity.

The ability of three proteases producing bacteria such as Pseudomonas aeruginosa, Serratia marcescens, and Bacillus pumilus on the demineralization and deproteinzation efficiency of shrimp waste, for chitin extraction, was investigated. Statistical analysis of data was showed a significant difference between the percentage of demineralization and deproteinization in different bacteria species (p<0.05). The highest deproteinization (74.76%) and demineralization rate (78.46%) was obtained with P. aeruginosa and the lowest was observed in the treatment of S. marcescens. Then, chitin was converted to chitosan by deacetylation in the presence of NaOH 50%. The antioxidant activity of chitosan solution was determined using different tests. The highest activity (DO 700 nm=0.74, DO 695 nm=0.31) was observed for chitosan sample at concentration of 1,000 µg/ml. The antioxidant potential of the hydrolysates was also evaluated. The highest reducing power in a volume of 400 µl hydrolysate of S. marcescens and the highest total antioxidant capacity in a volume 100 µl hydrolysate of B. pumilus were observed. These results indicated that the P. aeruginosa bacterium in comparison with other bacterial strains, higher ability to remove proteins and mineral from shrimp waste. Therefore, the use of this bacterium is recommended for protein and mineral removal from marine crustaceans.