miR-888 and E-Cadherin Levels in Ductal Carcinoma Breast Cancer as Possible Cancer-Related Markers.

Background: Cancer occurs due to genetic or epigenetic factors. Breast cancer (BC) is the most common cancer among females and is considered one of the leading causes of cancer death worldwide. Epigenetic biomarkers have proven to be important in cancer management. Methods: E-cadherin gene expression and its associated regulatory microRNA, miR-888, were studied in 60 ductal carcinoma BC patients compared with normal controls using real-time Reverse Transcription Polymerase Chain Reaction. In addition, the association of the gene and microRNA expression with hormone receptors and other clinicopathologic characteristics were assessed. The correlation between plasma and tumor miR-888 expression was studied to evaluate its potential as a marker of noninvasiveness. Results: The results showed a significant increase in expression of miR-888, and a decrease in E-cadherin expression in tumor tissues compared with normal controls. The miR-888 and E-cadherin mRNA levels showed an inverse correlation. The overexpression of miR-888 in both tumor and plasma and downregulation of E-cadherin were particularly prominent when there was nodal involvement and distant metastasis status as we well as being significantly associated with higher disease stages and triple-negative tumor hormone receptor status. The Kaplan-Meier survival curves indicated that overall survival was significantly poor in BC patients with higher miR-888 and lower E-cadherin expression. Conclusions: Our study indicated that elevated eXpression of miR-888 may be a negative prognostic factor in breast cancer.

Integrity and Quantity Evaluation of Plasma Cell-Free DNA in Triple Negative Breast Cancer.

BACKGROUND: Triple-Negative Breast Cancer (TNBC) is a subtype of breast cancer that lacks expression of the estrogen and progesterone receptor and does not overex-press human epidermal growth factor 2 receptor protein. TNBC is associated with special characteristics, including aggressiveness, poor prognosis, and treatment response. Non-invasive blood-based molecular markers such as cell-free DNA (cfDNA) variables have been shown to be putative markers in breast cancer prognosis. METHODS: The cfDNA quantity and integrity were assessed in a case-control study of 96 breast cancer patients including 46 triple negative and 50 non-triple negative compared with 50 unaffected controls. A quantitative real-time PCR approach based on the quantification of two amplicons of the ß-actin gene with different lengths (99 and 394 bp) was used to evaluate the integrity index 394/99. RESULTS: Both cfDNA integrity index and quality were significantly elevated in breast cancer patients but integrity index can be considered as the more reliable diagnostic marker. The statistically significant increase of cfDNA quantity and integrity was observed in TNBC patients, somehow associated with nodal metastasis (p<0.001). CONCLUSION: Elevated cfDNA concentration and integrity index in breast cancer patients compared with normal control and significant difference observed between TNBC and non-TNBC may be considered as a possible effective non-invasive diagnostic and prognostic molecular marker in breast cancer.