[Influence of malignant growth and chronic neurogenic pain on neurosteroid levels in rat brain]. / Vliianie zlokachestvennogo rosta i khronicheskoi neirogennoi boli na uroven' neirosteroidov v mozge krys.

The aim of the study was to determine the level of sex steroid hormones in white matter of the brain of rats with tumors combined with chronic neurogenic pain (CNP), which was modeled by bilateral sciatic nerve ligation. The study included albino male rats (n=74). In the main group, M1 sarcoma was transplanted subcutaneously (n=11) or into the subclavian vein (n=11) 45 days after CNP modeling. Two comparison groups (n=13 each) included sham operated animals (without CNP) with M1 sarcoma transplanted subcutaneously and intravenously. Control groups included animals with CNP and sham operated animals. Rats were euthanized on day 21 of the carcinogenesis. Levels of total and free testosterone (T), estrone (E1), estradiol (E2), estriol (E3) and progesterone (P4) in the brain white matter were measured using ELISA kits ("Cusabio", China). CNP caused a decrease in the total and free T by 1.5 times (p<0.05), E2 and P4 by 1.9 and 3 times, respectively, E3 by 1.6 times (p<0.05), as well as an increase in E1 by 1.4 times (p<0.05) as compared to the corresponding levels in the brain white matter of rats without CNP. CNP stimulated M1 sarcoma growth in both subcutaneous and intravenous transplantation. Regardless of the tumor site, the dynamics of total T, E2 and E3 in the brain had similar features, but the dynamics of free T, P4 and E1 differed. Thus, changes in the level of neurosteroids in the white matter of rat brain with CNP and tumor growth alone or associated with CNP are a reaction to stress.

[The level of endogenous intoxication iт oncologic gynecologic patients.]

The leading role of syndrome of endogenous intoxication in structure of clinical manifestations of tumor disease conditions actuality of studying components determining its development, in dynamics of malignant process and under anti-tumor therapy. Among all studied groups of oncogynecological patients, the most expressed decreasing of functional activity of albumin binding toxic compounds is observed under ascitic form of ovary cancer. In the group of patients with ovary cancer of stage I-II and also in condition of remission of process in patients with ovary cancer of stage III, the values of total and effective concentration of albumin and its binding capacity, on the contrary, are approaching to donor values. Under cervical carcinoma and vulva cancer the statistically significant decreasing of effective concentration of albumin and its binding capacity was established. However, thisdecreasing is manifested in minor degree than in patients with ascitic form of ovary cancer.

Experimental study of structural, functional, and biochemical changes in immune organs under conditions of antitumor activity of copper nanoparticles.

The effects of copper nanoparticles on the structure and function of the immune system organs (thymus and spleen) and intensity of free radical processes in the spleens of rats with sarcoma 45 were studied. A relationship between morphological and biochemical changes and antitumor efficiency of copper nanoparticles was demonstrated.