Comparative Evaluation of Two Esthetic Full Coronal Restorative Materials for Primary Incisors.

Aim: This study was designed to compare the clinical efficacy of two esthetic restorative materials, nanoceramic (Magma NT®) and giomer (Beautifil II®), as full coronal restoration in primary maxillary incisors. Materials and methods: A total of 15 patients aged 3-5 years presenting with mutilated primary maxillary incisors due to caries or trauma were selected for the study using randomized simple sampling. A total of 40 maxillary incisors were randomly divided into two equal groups, with 20 teeth in each group. Teeth in group I (GP I) were restored with nanoceramic (Magma NT®) and group II (GP II) with giomer (Beautifil II®). The full coronal restorations were done using strip crowns (3M ESPE). The restorations were evaluated for gross fracture, marginal integrity, and secondary caries according to modified Ryge's criteria [United States Public Health Service (USPHS)] at baseline (immediate postoperative), 3, 6, and 9 months. Parental satisfaction with each type of restoration was also evaluated using the Likert 5-point scale. Results: The data obtained was statistically analyzed using the Chi-squared test, and the level of significance, that is, the p-value, was determined. The Chi-squared test showed no significant changes to all modified USPHS criteria for each material at baseline and 3-month evaluation period. The changes recorded were after a 3-month follow-up between the two materials; nanoceramic (Magma NT®) restoration demonstrated marginally better than giomer (Beautifil II®) in terms of gross fracture and marginal integrity; however, there was no statically significant difference between them (p > 0.05), while giomer (Beautifil II®) was better than nanoceramic in terms of secondary caries (p < 0.05). Parental satisfaction for both entities was comparable in terms of color and durability; however, they were cost-ineffective. Conclusion: Nanoceramic restoration demonstrated better results in terms of gross fracture and marginal integrity, while giomer was better in terms of secondary caries. Clinical significance: Nanoceramics and giomers can serve as an alternative to conventional restorative materials in primary anterior teeth because of their improved qualities. How to cite this article: Dhaker KK, Tandon S, Rathore AS, et al. Comparative Evaluation of Two Esthetic Full Coronal Restorative Materials for Primary Incisors. Int J Clin Pediatr Dent 2024;17(3):321-327.

Facile synthesis of Ag NPs@MgO nanosheets for quantitative SERS-based detection and removal of hazardous organic pollutants.

Surface-enhanced Raman spectroscopy (SERS) is a highly precise and non-invasive analytical method known for its ability to detect vibrational signatures of minute analytes with exceptional sensitivity. However, the efficacy of SERS is subject to substrate properties, and current methodologies face challenges in attaining consistent, replicable, and stable substrates to regulate plasma hot spots across a wide spectral range. This study introduces a straightforward and economical approach that incorporates monodispersed silver nanoparticles onto 2-D porous magnesium oxide nanosheets (Ag@MgO-NSs) through an in-situ process. The resulting nanocomposite, Ag@MgO-NSs, demonstrates substantial SERS enhancement owing to its distinctive plasmonic resonance. The effectiveness of this nanocomposite is exemplified by depositing diverse environmental pollutants as analytes, such as antibiotic ciprofloxacin (CIP), organic dyes like rhodamine 6G (R6G) and methylene blue (MB), and nitrogen-rich pollutant like melamine (MLN), onto the proposed substrate. The proposed nanocomposite features a 2-D porous structure, resulting in a larger surface area and consequently providing numerous adsorption sites for analytes. Moreover, engineering the active sites of the nanocomposite results in a higher number of hotspots, leading to an enhanced performance. The nanocomposite outperforms, exhibiting superior detection capabilities for R6G, MB, and MLN at concentrations of 10-6 M and CIP at concentration of 10-5 M, with impressive uniformity, reproducibility, stability, and analytical enhancement factors (EF) of 6.3 x 104, 2 x 104, 2.73 x 104 and 1.8 x 104 respectively. This approach provides a direct and cost-effective method for the detection of a broad spectrum of environmental pollutants and food additives, presenting potential applications across diverse domains. The detected environmental pollutants and food additives are removed through both catalytic degradation (R6G and MB) and adsorption (CIP and MLN).

Numerical simulation for MHD Oldroyd-B fluid flow with melting and slip effect.

This investigation reflects an examination of Oldroyd-B fluid flow over a permeable surface subjected to the effects of melting, slip effect, inclined magnetic field and chemical reactions. The governing equations are resolved using the bvp4c inbuilt MATLAB tool, the arithmetic computation for the momentum, thermal and concentration equations are executed. The results are exhibited graphically. Numerical outcomes are graphically depicted by aid of velocity, concentration, temperature profiles for several model variables. The achieved results exhibit a promising agreement with the previously established findings available in the open literature. The results obtained indicated that Deborah number ß 1 reduces the momentum boundary layer thickness whereas Deborah number ß 2 enhances the adjacent momentum boundary layer. Furthermore, temperature profile declined for melting parameter Me . The application of this study transcends various engineering disciplines, offering practical solutions and optimization opportunities in polymer processing, coating technologies, cooling systems, materials processing, biomedical and environmental engineering.

Localization of RNAs to the mitochondria-mechanisms and functions.

The mammalian mitochondrial proteome comprises over 1000 proteins, with the majority translated from nuclear-encoded messenger RNAs (mRNAs). Mounting evidence suggests many of these mRNAs are localized to the outer mitochondrial membrane (OMM) in a pre- or cotranslational state. Upon reaching the mitochondrial surface, these mRNAs are locally translated to produce proteins that are cotranslationally imported into mitochondria. Here, we summarize various mechanisms cells use to localize RNAs, including transfer RNAs (tRNAs), to the OMM and recent technological advancements in the field to study these processes. While most early studies in the field were carried out in yeast, recent studies reveal RNA localization to the OMM and their regulation in higher organisms. Various factors regulate this localization process, including RNA sequence elements, RNA-binding proteins (RBPs), cytoskeletal motors, and translation machinery. In this review, we also highlight the role of RNA structures and modifications in mitochondrial RNA localization and discuss how these features can alter the binding properties of RNAs. Finally, in addition to RNAs related to mitochondrial function, RNAs involved in other cellular processes can also localize to the OMM, including those implicated in the innate immune response and piRNA biogenesis. As impairment of messenger RNA (mRNA) localization and regulation compromise mitochondrial function, future studies will undoubtedly expand our understanding of how RNAs localize to the OMM and investigate the consequences of their mislocalization in disorders, particularly neurodegenerative diseases, muscular dystrophies, and cancers.

Step by step enhancement of aesthetics for distal phalangeal prosthetic replacement using neoteric stamp technique: A case report.

This case study delineates the proficient creation of a silicone finger prosthesis, tailored for a patient contending with partial digit amputation. The prosthesis was devised with the overarching goal of reinstating not only the physiological dexterity of the hand but also its aesthetic integrity and the patient's psychological equilibrium. The crafting process entailed a meticulous technique to replicate the intricate texture of the skin in order to guarantee a near normal appearance. Post-prosthesis integration, the patient exhibited enhancements in manual functionality and articulated a heightened self-assuredness because of the indiscernible prosthesis. This illustrative case underscores the efficacy of silicone finger prosthetics in conferring both functional and aesthetic restitution to those afflicted with partial digit amputations.

A study on effects of cell phone tower-emitted non-ionizing radiations in an Allium cepa test system.

Considering enormous growth in population, technical advancement, and added reliance on electronic devices leading to adverse health effects, in situ simulations were made to evaluate effects of non-ionizing radiations emitted from three cell phone towers (T1, T2, and T3) of frequency bands (800, 1800, 2300 MHz), (900, 1800, 2300 MHz), and (1800 MHz), respectively. Five sites (S1-S5) were selected near cell phone towers exhibiting different power densities. The site with zero power density was considered as control. Effects of radiations were studied on morphology; protein content; antioxidant enzymes like ascorbate peroxidase (APX), superoxide dismutase (SOD), glutathione-S-transferase (GST), guaiacol peroxidase (POD), and glutathione reductase (GR); and genotoxicity using Allium cepa. Mean power density (µW/cm2) was recorded as 1.05, 1.18, 1.6, 2.73, and 12.9 for sites 1, 2, 3, 4, and 5, respectively. A significant change in morphology, root length, fresh weight, and dry weight in Allium cepa was observed under the exposure at different sites. Protein content of roots showed significant difference for samples at all sites while bulbs at sites S4 and S5 when compared to control. Antioxidant activity for root in terms of APX, GST, and POD showed significant changes at S4 and S5 and GR at site S5 and SOD at S1, S2, S3, S4, and S5. Similarly, bulbs showed significant changes at sites S4 and S5 for APX while at sites S3, S4, and S5 for POD and S2, S3, S4, and S5 for SOD and S5 for GR and GST. Genotoxicity study has shown induction of abnormalities at different stages of the cell cycle in Allium cepa root tips. The samples under exposure to radiation with maximum power density have shown maximum induction of oxidative stress and genotoxicity.

Nanotechnological advances in the treatment of epilepsy: a comprehensive review.

Epilepsy is one of the most prevalent chronic neurological disorders characterized by frequent unprovoked epileptic seizures. Epileptic seizures can develop from a broad range of underlying abnormalities such as tumours, strokes, infections, traumatic brain injury, developmental abnormalities, autoimmune diseases, and genetic predispositions. Sometimes epilepsy is not easily diagnosed and treated due to the large diversity of symptoms. Undiagnosed and untreated seizures deteriorate over time, impair cognition, lead to injuries, and can sometimes result in death. This review gives details about epilepsy, its classification on the basis of International League Against Epilepsy, current therapeutics which are presently offered for the treatment of epilepsy. Despite of the fact that more than 30 different anti-epileptic medication and antiseizure drugs are available, large number of epileptic patients fail to attain prolonged seizure independence. Poor onsite bioavailability of drugs due to blood brain barrier poses a major challenge in drug delivery to brain. The present review covers the limitations with the state-of-the-art strategies for managing seizures and emphasizes the role of nanotechnology in overcoming these issues. Various nano-carriers like polymeric nanoparticles, dendrimers, lipidic nanoparticles such as solid lipid nanoparticles, nano-lipid carriers, have been explored for the delivery of anti-epileptic drugs to brain using oral and intranasal routes. Nano-carries protect the encapsulated drugs from degradation and provide a platform to deliver controlled release over prolonged periods, improved permeability and bioavailability at the site of action. The review also emphasises in details about the role of neuropeptides for the treatment of epilepsy.

The cyanobacterial FtsH4 protease controls accumulation of protein factors involved in the biogenesis of photosystem I.

Membrane-bound FtsH proteases are universally present in prokaryotes and in mitochondria and chloroplasts of eukaryotic cells. These metalloproteases are often critical for viability and play both protease and chaperone roles to maintain cellular homeostasis. In contrast to most bacteria bearing a single ftsH gene, cyanobacteria typically possess four FtsH proteases (FtsH1-4) forming heteromeric (FtsH1/3 and FtsH2/3) and homomeric (FtsH4) complexes. The functions and substrate repertoire of each complex are however poorly understood. To identify substrates of the FtsH4 protease complex we established a trapping assay in the cyanobacterium Synechocystis PCC 6803 utilizing a proteolytically inactivated trapFtsH4-His. Around 40 proteins were specifically enriched in trapFtsH4 pulldown when compared with the active FtsH4. As the list of putative FtsH4 substrates contained Ycf4 and Ycf37 assembly factors of Photosystem I (PSI), its core PsaB subunit and the IsiA chlorophyll-binding protein that associates with PSI during iron stress, we focused on these PSI-related proteins. Therefore, we analysed their degradation by FtsH4 in vivo in Synechocystis mutants and in vitro using purified substrates. The data confirmed that FtsH4 degrades Ycf4, Ycf37, IsiA, and also the individual PsaA and PsaB subunits in the unassembled state but not when assembled within the PSI complexes. A possible role of FtsH4 in the PSI life-cycle is discussed.